SENSORS
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2020
·Vol. 20
·期 5
·1501
·
IF 3.275
Jingzhou Hou,Huixiang Wu,Xin Shen,Chao Zhang,Changjun Hou,Qiang He,Danqun Huo
A facile and effective colorimetric-sensing platform based on the diazotization of phenosafranin for the detection ofNO2−under acidic conditions using the Griess assay is presented. Diazotization of commercial phenosafranin produces a color change from purplish to blue, which enables colorimetric quantitative detection ofNO2−. Optimal detection conditions were obtained at a phenosafranin concentration of 0.25 mM, HCl concentration of 0.4 M, and reaction time of 20 min. Under the optimized detection conditions, an excellent linearity range from 0 to 20 μM was obtained with a detection limit of 0.22 μM. Favorable reproducibility and selectivity of the colorimetric sensing platform towardNO2−were also verified. In addition, testing spiked ham sausage, bacon, and sprouts samples demonstrated its excellent practicability. The presented colorimetric sensing platform is a promising candidate for the detection ofNO2−in real applications.Keywords:Griess assay;colorimetric;nitrite;phenosafranin
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Jove-Journal of Visualized Experiments
·
2019
·
IF 1.108
Yalan Wu,Shengjun Wu,Xin-an Huang,Qingping Zeng,Tao Deng,Fang Liu
Primaquine (PMQ), an important anti-malarial drug, has been recommended by the World Health Organization (WHO) for the treatment of life-threatening infections caused by P. vivax and ovale. However, PMQ has unwanted adverse effects that lead to acute hemolysis in patients with glucose-6-phosphate dehydrogenase (G6PD) deficiency. There is a need to develop simple and reliable methods for PMQ determination with the purpose of dosage monitoring. In early 2019, we have reported an UV-Vis and naked-eye based approach for PMQ colorimetric quantification. The detection was based on a Griess-like reaction between PMQ and anilines, which can generate colored azo products. The detection limit for direct measurement of PMQ in synthetic urine is in the nanomolar range. Moreover, this method has shown great potential for PMQ quantification from human serum samples at clinically relevant concentrations. In this protocol, we will describe the technical details regarding the syntheses and characterization of colored azo products, the reagent preparation, and the procedures for PMQ determination.
研究领域:
药物分析
·
医学诊断
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 19
·4751-4761
·
IF 4.157
Cai Rongyuan,Lu Dechan,She Qiutian,You Ruiyun,Feng Shangyuan,Lin Xueliang,Lu Yudong
When nitrite is ingested and absorbed by the body, it can be converted into highly toxic nitrosamines (carcinogens, teratogens, and mutagens), posing health risks to the general population. Therefore, it calls for establishing a method for determination of nitrite. In this paper, the glass-SiO 2 -Ag surface-enhanced Raman scattering (SERS) substrate with a large number of “hot spots” were prepared by two kinds of silane coupling agents. The SERS substrate had high sensitivity and repeatability. Silicon dioxide supported the silver nanoparticles (Ag NPs), which increased surface roughness of the substrate, generated a great quantity of hot spots and enhanced the SERS signal. In the SERS spectrum, the intensity ratio of the two characteristic peaks (1287 cm −1 and 1076 cm −1 ) had a good linear correlation with the logarithm of the concentration of nitrite, R 2 = 0.9652. The recoveries of 50 μM and 100 μM nitrite in three kinds of foods, three kinds of cosmetics and tap water were 90.9–105.3%. Graphical abstract
研究领域:
分析化学
·
环境科学
·
食品安全
✓ 已验证
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Huixiang Wu,Xin Shen,Danqun Huo,Yi Ma,Minghong Bian,Caihong Shen,Changjun Hou
In this work, we presented a sensitive and selective colorimetric and fluorescent dual-readout sensor based on Griess assay for nitrite ( NO 2 − ) detection under acidic condition. The sensor system was constituted of acid-resistant carbon quantum dots (CQDs) and 3-aminophenol (3-Aph) with acidic condition regulated using HCl. During the sensing procedure, reaction of 3-Aph and NO 2 − under acidic condition can yield a yellow-colored azoic compound (AZO), which gives the colorimetric readout; meanwhile, the fluorescence of CQDs (fluorescence reporter) quenched due to the strong absorption of AZO, leading to fluorescent readout. Wherein, CQDs were synthesized via hydrothermal method through using polyacrylamide as precursor and characterized by AFM, XRD and XPS . Under the optimized condition, the sensor exhibit broad linear relationships towards NO 2 − in the range of 10 to 100 nM and 2.5 to 100 μM, with practical detection limits of 10 nM and 2.5 μM for the fluorescent and colorimetric readout, respectively. And the sensor displayed excellent capability of selectivity according to interferences study. Furthermore, testing of sprouts, bacon and ham sausage real samples demonstrated good recoveries and reproducibility of the sensor system. All these results suggested the presented colorimetric and fluorescent dual-readout sensor can be a promising candidate for the NO 2 − detection in real applications.
研究领域:
分析化学
·
生物传感
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）