Emerging Microbes & Infections
·
2024
·
IF 8.4
Yaqin Peng,Yue Liu,Xuegao Yu,Jingchun Fang,Zhaowang Guo,Kang Liao,Peisong Chen,Penghao Guo
Candida auris is an emerging multidrug-resistant fungal pathogen worldwide. To date, it has not been reported in Guangdong, China. For the first time, we reported 7 cases of C. auris candidemia from two hospitals in Guangdong. The clinical and microbiological characteristics of these cases were investigated carefully. Two geographic clades, i.e. III and I, were found popular in different hospitals by whole genome sequencing analyses. All C. auris isolates from bloodstream were resistant to fluconazole, 5 of which belonged to Clade III harbouring VF125AL mutation in the ERG11 gene. The isolates with Clade I presented Y132F mutation in the ERG11 gene as well as resistance to amphotericin B. All isolates exhibited strong biofilm-forming capacity and non-aggregative phenotype. The mean time from admission to onset of C. auris candidemia was 39.4 days (range: 12 - 80 days). Despite performing appropriate therapeutic regimen, 42.9% (3/7) of patients experienced occurrences of C. auris candidemia and colonization after the first positive bloodstream. C. auris colonization was still observed after the first C. auris candidemia for 81 days in some patient. Microbiologic eradication from bloodstream was achieved in 85.7% (6/7) of patients at discharge. In conclusion, this study offers a crucial insight into unravelling the multiple origins of C. auris in Guangdong, highlighting great challenges in clinical prevention and control.
研究领域:
医学真菌学
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感染病学
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抗真菌药物研究
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DOI
PubMed
高影响因子
Water
·
2025
·Vol. 17
·期 4
·574
·
IF 3
Yanjing Zhu,Sijia Yang,Mathias Gustavsson,Wenli Huang,Si Gao,Rui Wang
Wastewater treatment plants (WWTPs) play a crucial role in mitigating microplastic (MP) release to the environment. In this paper, a WWTP of a textile manufacturing plant in Guangdong, China, was investigated to identify MP characteristics and the effectiveness of wastewater treatment within the plant. Laser Direct Infrared (LDIR) and Liquid Chromatography with Mass Spectrometry (LC-MS/MS) were applied to quantify both the number and the mass of the microplastics in the effluent of the textile manufacturing plant where most of the wastewater were from three printing and dyeing lines. The study further investigated the MP removal efficiency of each wastewater treatment process of the industry-owned WWTP and analysed the removal mechanism of each step, highlighting limitations in detecting and eliminating MPs. It is observed that (1) the results from LDIR and LC-MS/MS can be complementary to each other; (2) the MP concentration in the influent was 1730 n/L by number and 13.52 µg/L by mass; (3) the total removal efficiency of the WWTP were 99% by the number of MPs and 67.7% by the mass of MPs; (4) nine types of polymers have been identified in the influent, of which Polyamide (PA) was dominating; (5) hydrolysis acidification removed PA most; (6) aerobic tank, sand filter, and biological aerated filter (BAF) showed low removal efficiency; (7) coagulation and sedimentation tank had the highest removal efficiency to PET than any other processes.
研究领域:
环境科学
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废水处理
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DOI
高影响因子
Food Analytical Methods
·
2025
·Vol. 19
·期 1
·42
·
IF 3
Bi Siyuan,Zhang Ji,Jiang Hongmeng,Chen Ziqian,Jiang Hao,Dong Hao,Qian Min,Bai Weidong
Volatile flavor compounds in rice wine were analyzed using gas chromatography-ion mobility chromatography (GC-IMS). The optimal analytical conditions were determined by systematically optimizing the incubation parameters of Huangjiu. The results revealed that the optimal incubation conditions were 60 °C, 15 min, and 0.6 g NaCl. Furthermore, 31 volatile compounds were identified, including esters, alcohols, aldehydes, ketones, and miscellaneous monomers. Subsequently, semiquantitative analysis showed esters were the predominant group, followed by alcohols. Comparative analysis of different Huangjiu varieties revealed distinct differences and underlying similarities in their characteristic volatile flavor compounds, providing valuable theoretical insights into the differentiation of Guangdong Huangjiu varieties. Graphical
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高影响因子
Food & Function
·
2025
·
IF 5.4
Shuqia Ding,Linfen He,Wenjing Pan,Qingqing Ye,Hanzhong Ye,Zhonghua Liu,Shuxian Cai
‘Hongyin’ (HY) is a purple-budded Camellia sinensis cultivar from eastern Guangdong whose chemistry and bioactivities are not fully defined. We integrated sensory and compositional assays (catechins, caffeine, anthocyanins) with UPLC–MS/MS broad-targeted metabolomics, KEGG enrichment and network pharmacology, and evaluated bioactivity in high-fat-diet (HFD) mice with gut-microbiota (16S rRNA) profiling and microbiota–metabolite correlations. HY showed a distinctive chemical phenotype—low caffeine (most samples <1%), elevated gallocatechin gallate (GCG), anthocyanin enrichment, and predominance of linoleic/α-linolenic-acid (LA/ALA)–related unsaturated lipids; storage coincided with reduced astringency and orange-red liquor. Metabolomics annotated 2,105 metabolites, of which 1,311 differed from a green-tea control (GT), enriched in LA/ALA metabolism and unsaturated-fatty-acid biosynthesis. In HFD mice, HY from week 5 mitigated weight gain and fasting glycaemia, reduced hepatic lipid droplets and inflammatory infiltration, and improved intestinal morphology (higher villus/crypt ratio) and colonic histology. Microbiota analyses revealed recovery of α-diversity, β-diversity convergence toward normal diet, a lower Firmicutes/Bacteroidetes ratio, depletion of Proteobacteria/Enterobacteriaceae and Helicobacter, and enrichment of Akkermansia, Bacteroides, Faecalibacterium, Blautia, and Lactobacillus. Correlation heatmaps linked tea polyphenols (GCG, anthocyanins) and LA/ALA-axis lipids positively with these beneficial taxa and negatively with putative pro-inflammatory groups. Collectively, HY combines a low-caffeine, high-GCG/anthocyanin, UFA-enriched profile with multi-dimensional bioactivities—anti-obesity, hepatoprotective, anti-inflammatory and microbiota-modulating—supporting its quality evaluation and functional development as a regional tea resource.
研究领域:
植物科学
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食品化学
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代谢组学
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营养生物化学
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生药学
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微生物组研究
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功能食品
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天然产物
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2017
·
IF 4.529
Li Ma,Lin Wang,Jie Tang,Zhaoguang Yang
Arsenic speciation and heavy metal distributions have been investigated in locally grown rice grains from Guangdong Province, Southern China. A total of 41 polished rice grain samples were collected throughout Guangdong Province. Arsenite (As(III)), as the predominant form found in the rice, was positively correlated ( p < 0.01) with total As (tAs) concentration. However, the percentage of As(III) reduced while tAs concentration increased (r = −0.361, p < 0.05), due to restricted accumulation and translocation of As(III) in rice grains at high level of tAs. Statistical and geostatistical analyses were applied to investigate potential origins of heavy metals in rice. Only Cd, Cu and Ni were identified as influenced by anthropogenic sources such as industrial and commercial activities. As and Pb were primarily controlled by natural occurrence. The results of health risk assessment implied that continuous intake of rice grown in Guangdong Province could cause considerably non-carcinogenic and carcinogenic risk to local inhabitants.
研究领域:
环境科学
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食品科学
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毒理学
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PubMed
高影响因子
Horticulturae
·
2026
·Vol. 12
·期 9
·1165
·
IF 3.4
Genlin Mao,Diyang Qiu,Wanbing Chen,Jiwu Zeng
Citrus medica‘Guangdong Xiangshui’ is a commercially valuable specialty citrus cultivar whose leaf volatile organic compounds (VOCs) hold promise for applications in food flavor development. Although rootstock genotype is known to influence secondary metabolism in scions, its specific regulatory effects on leaf VOCs in this cultivar remain poorly understood. In this study, headspace gas chromatography–ion mobility spectrometry (HS-GC-IMS) combined with multivariate statistical analysis was employed to profile leaf VOCs from plants grafted onto four different rootstocks, alongside own-rooted controls. For comparative purposes, leaf and pericarp VOC profiles were also analyzed under identical conditions. A total of 43 valid ion signals were detected, of which 27 neutral parent volatile compounds—predominantly aldehydes, alcohols, ketones, esters, and sulfur-containing compounds—were tentatively identified via database matching (match score > 80%). Distinct volatile fingerprints were observed among rootstock genotypes and propagation modes, and nine candidate differential compounds with variable importance in projection (VIP) > 1 were screened, demonstrating that HS-GC-IMS coupled with exploratory chemometric analysis enables preliminary discrimination of leaf samples across rootstock treatments. Comparative tissue analysis revealed pronounced metabolic divergence: leaves accumulated abundant C6 aldehydes and alcohols, indicative of lipoxygenase (LOX) pathway activity, whereas pericarp exhibited a higher proportion of terpenoids, consistent with terpene synthase (TPS) pathway dominance. However, these metabolic profiling results alone do not conclusively establish pathway-mediated regulatory mechanisms, necessitating further molecular and biochemical investigations. Collectively, this study provides foundational data for rootstock selection and the utilization of leaf aroma resources inC. medica‘Guangdong Xiangshui’.
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高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2019
·
IF 5.589
Pengfei Wu,Yuanyuan Tang,Miao Dang,Siqing Wang,Hangbiao Jin,Yunsong Liu,Hao Jing,Chunmiao Zheng,Shuping Yi,Zongwei Cai
Concerns over the negative impacts of microplastics on human health have led to growing attention on the occurrence of microplastics in aquatic environment. Recent studies have extended their focus from marine to inland waters, especially on the spatial-temporal distribution of the microplastics in urban rivers. In this study, Maozhou River, the largest river in Shenzhen, a tributary of the Pearl River, was selected as a representative inland waterway of Guangdong-Hong Kong-Macao Greater Bay Area. The spatial-temporal investigation was performed on microplastics in the surface water and sediments of 17 sites along the mainstream of the Maozhou River. Results show that microplastics were widely and unevenly distributed along the river and reached the high abundances on the site most intensively surrounded by industries as well as the sites downstream. The abundances in dry season ranged from 4.0 ± 1.0 to 25.5 ± 3.5 items·L −1 in water and 35 ± 15 to 560 ± 70 item·kg −1 in sediments, which were relatively higher than those observed in the wet season (water: 3.5 ± 1.0 to 10.5 ± 2.5 items·L −1 ; sediments: 25 ± 5 to 360 ± 90 item·kg −1 ; p value < 0.05). The dominant types of the microplastics were identified as: PE Polyethylene (PE, water: 45.0%, sediments: 42.0%), polypropylene (PP, water and sediments: 12.5%), polystyrene (PS, water: 34.5%; sediments 14.5%) and polyvinyl chloride (PVC, water: 2.0%; sediments: 15%). Moreover, metals like Al, Si, Ca were discovered on the rough surface of the microplastics, indicating the interactions between the microplastics and the aquatic environment. Through a comprehensive comparison with other major inland waters in China, this work provides valuable data on the microplastics pollution of a representative inland water in the Greater Bay Area, and will further contribute to a better understanding on the land-based input of microplastics from the intensively affected inland waters.
研究领域:
环境科学
·
生态学
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高影响因子
Sustainability
·
2021
·Vol. 13
·期 22
·12505
·
IF 3.251
Sung-Lin Hsueh,Yuan Feng,Yue Sun,Ruqi Jia,Min-Ren Yan
Rural areas in southern China receive ample rainfall annually as well as over 1600 h of annual sunshine. Despite a generally severe urban–rural development imbalance, these rural areas feature well-developed basic infrastructure and diverse economic activities. Rural revitalization policies in these areas have emphasized the development of cultural and ecological tourism, which has spurred economic development and given rise to a trend of villa construction. Residential buildings sit on large areas where natural resources are abundant. These advantages are conducive to the development and use of sustainable resources. This study proposes an incentive policy encouraging rural residents to renovate their buildings to include rainwater conservation and solar power generation. The Delphi method, an analytic hierarchy process, and fuzzy logic theory were combined to establish an AI-MCDM model, with applications of artificial intelligence and multiple-criteria decision making. Using Conghua District, Guangdong Province as an example, the study suggested that the model is beneficial to increasing the willingness of rural residents to reconstruct and renovate their residences, promoting the development of a low-carbon ecological region, Wenquan Township. We conducted the Delphi process twice to assess and validate incentives for installing natural resource conservation structures in agricultural areas. Nine criteria were identified, which can be divided into three main dimensions of participation situation, generating capacity, and storage facilities. The proposed AI-MCDM model developed using the Delphi–Fuzzy Analytic Hierarchy Process Model has high objectivity and can support rural areas in developing low-carbon, sustainable characteristics. The findings can serve as a reference for governments formulating incentives to encourage the installation of rainwater conservation and solar energy generation structures by rural households.
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高影响因子
ACS Sensors
·
2026
·
IF 10.9
Tianchi Zhuang,Yan Long,Yongtong Xu,Qiumei Li,Zhiming Liu,Xueshan Zhong,Dawei Wu,Yu Ma,Wenzhe Su,Xiaokang Li,Zunfu Ke,Cheng Guo
Early and accurate diagnosis of Chikungunya virus (CHIKV) infection is critical for controlling its outbreaks. CRISPR/Cas-based detection offers promise for pathogen identification, yet one-step CRISPR/Cas systems are limited by suboptimal sensitivity, field-deployability, and adaptability to complex clinical samples, hindering their use in rapid outbreak response. Here, we developed a CRISPR/Cas13a-based One-Step System for Rapid Detection of Emerging Viruses (CRISPR-CORE) and applied it during the CHIKV outbreak in Guangdong Province, China. Multidimensional optimizations enabled the CRISPR-CORE system to achieve a limit of detection of 5 copies/μL within 40 min. An extraction-free RNA release protocol for CHIKV in blood samples and a premixed reagent approach were implemented. Furthermore, a portable fluorescence detector was used to enhance user-friendliness in point-of-care (POC) settings. The clinical CHIKV genomic information was identified through hybrid capture sequencing, informing the design of highly specific CRISPR RNA (crRNA). Clinical validation across three regions yielded 92.6% sensitivity and 100% specificity, underscoring the applicability and reliability of the CRISPR-CORE system. Our system demonstrates its suitability for CHIKV outbreak detection. It facilitates rapid and POC testing for emerging viruses in resource-limited settings. Furthermore, it provides a universal strategy for the prevention and control of infectious diseases.
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DOI
PubMed
高影响因子
FOOD CONTROL
·
2018
·
IF 3.667
Hui Liang,Wei-Liang Wu,Yong-Hui Zhang,Shao-Jun Zhou,Chao-Yang Long,Jian Wen,Bo-Yuan Wang,Zhi-Ting Liu,Chao-Zheng Zhang,Pan-Pan Huang,Ning Liu,Xiao-Ling Deng,Fei Zou
To systematically investigate the presence of toxic heavy metals and evaluate the potential health risks of heavy metals (HMs), the levels of cadmium (Cd), lead (Pb), arsenic (As), mercury (Hg), and chromium (Cr) in a total of 4401 representative samples of highly consumed fresh vegetables sampled from 21 prefecture-level cities of Guangdong Province, China, were determined by inductively coupled plasma mass spectrometry (ICP-MS). In the analyzed vegetables, the heavy metal concentrations exceeded the maximum allowable concentrations (MACs) set by Chinese legislation were of 2.41% for Cd, 1.61% for Pb, 0.55% for As, and 0.48% for Cr. The concentrations of HMs indicated significant differences ( p < 0.05) in vegetable groups and regions. Cd, and Pb were the major contaminants in vegetables according to the results of principal component analysis. During the four-year survey, a temporary rising trend was observed in As and Cr. The results from hierarchical cluster analysis also demonstrated that the status of HMs contaminations in vegetables from various regions displayed certain changes over time. The health risks associated with the intake of these HMs via vegetable consumption were evaluated in term of estimated daily intake (EDI), and noncarcinogenic and carcinogenic risks by target hazard quotient (THQ) and carcinogenic risk (CR). EDI values of each HM at both common and high consumption levels were observed to be less than the corresponding provisional tolerable daily intakes. The THQs of individual HMs and total target hazard quotients (TTHQs) were less than 1, indicating low health hazards for local residents. However, high consumption of stalk and stem vegetables, leafy vegetables , and root and tuber vegetables, and high consumption of vegetables marketed in the northern region (NR) of Guangdong Province resulted in TTHQs that approach 1 and may cause health risks to increase. The carcinogenic risks (CRs) of Cd, Pb and As were below the threshold level (10 −6 ) or residual level (10 −4 ), suggesting low potential CR from these HM exposures via vegetables consumption. However, highly consumed vegetable categories with high Cd concentrations and vegetables marketed in NR resulted in the CRs of Cd and TCRs exceeding the acceptable range, indicating that serious potential carcinogenic risks to human health may exist. In summary, the findings of this study reveal safe exposures of HMs via vegetable consumption, however, great concern should be focused on the noncarcinogenic and carcinogenic risks associated with high exposures of HMs, especially Cd, Pb and As, via high vegetable intake.
研究领域:
环境科学
·
食品安全
·
公共卫生
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DOI
高影响因子
危险品化学品经营许可证（带存储）