JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
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IF 5.895
Yuan Shi,Xiaokang An,Binbin Zhang,Xinglu Pan,Xiaohu Wu,Jun Xu,Wensheng Xiang,Fengshou Dong,Xiangjing Wang,Yongquan Zheng
Guvermectin is a biopesticide isolated from the secondary metabolites of Streptomyces sp. NEAU6, an endogenous actinomyces of a Chinese medicine named Paris polyphylla. However, the environmental degradation behavior and biotoxicity of guvermectin are still unclear, which may affect its rational application. Therefore, the degradation of guvermectin in water at different pH values (pH 4, pH 6, pH 7, and pH 9) and with or without light was investigated in the laboratory. The results showed that guvermectin could be degraded in pH 4 solution, and the presence of light irradiation enhanced the degradation process with a DT50 of 2.95 and 12 days for photolysis and hydrolysis, respectively. However, guvermectin was fairly stable in other conditions. Three products transformed from guvermectin degradation were identified by UPLC-QTOF/MS. Biotoxicity assessment was performed on Danio rerio and Daphnia magna Straus by ECOSAR prediction and in vivo biological tests. The test data showed that guvermectin and its transformation products exhibited low toxicities to D. rerio and D. magna Straus (LC50/EC50 > 100 mg a.i./L), and the transformation products had lower toxicity than their parent substance. The results provided a reference for elucidating the potential risk of guvermectin to nontarget organisms and promoting its rational use.
研究领域:
环境科学
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毒理学
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农药学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Peng Sun,Wenhui Yin,Chengcheng Jiang,Wentian Lu,Tianhe Liu,Yiyi Shao,Chongxi Liu,Jie Chen,Wensheng Xiang,Xiangjing Wang
Guvermectin (GV), a novel plant growth regulator from rhizosphere microorganism, has been officially registered as a new biopesticide in China. It plays important roles in regulating plant root growth and development, while the underlying mechanisms remain largely unknown. In this study, we demonstrate that GV induces auxin accumulation in Arabidopsis root tips and regulates root architecture through an auxin signaling-dependent pathway. Mechanistic investigation revealed that GV activates the indole-3-pyruvic acid biosynthetic pathway of auxin biosynthesis by upregulating the expression of YUC family genes, thereby increasing endogenous indole-3-acetic acid levels in the root tip. Concurrently, GV modulates polar auxin transport by downregulating PIN2 protein abundance, thereby reducing basipetal auxin efflux and promoting localized auxin retention. Collectively, these findings elucidate a dual regulatory mechanism whereby GV coordinates auxin biosynthesis and transport to optimize root growth. Our work bridges the gap between its agronomic application and molecular mechanism, offering a theoretical foundation for its integration into precision agriculture.
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
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IF 5.7
Yuan Shi,Xinglu Pan,Xiaohu Wu,Jun Xu,Wensheng Xiang,Yongquan Zheng,Fengshou Dong,Xiangjing Wang
Guvermectin, as a novel nucleoside-like biopesticide, could increase the rice yield excellently, but the potential environmental behaviors remain unclear, which pose potential health risks. Therefore, the uptake and biotransformation of guvermectin in three types of crops (rice, lettuce, and carrot) were first evaluated with a hydroponic system. Guvermectin could be rapidly absorbed and reached equilibrium in roots (12–36 h) and shoots (24–60 h) in three plants, and guvermectin was also vulnerable to dissipation in roots (t1/2 1.02–3.65 h) and shoots (t1/2 9.30–17.91 h). In addition, 8 phase I and 2 phase II metabolites, transformed from guvermectin degradation in vivo and in vitro exposure, were identified, and one was confirmed as psicofuranine, which had antibacterial and antitumor properties; other metabolites were nucleoside-like chemicals. Molecular simulation and quantitative polymerase chain reaction further demonstrated that guvermectin was metabolized by the catabolism pathway of an endogenous nucleotide. Guvermectin had similar metabolites in three plants, but the biotransformation ability had a strong species dependence. In addition, all the metabolites exhibit neglectable toxicities (bioconcentration factor <2000 L/kg b.w., LC50,rat > 5000 mg/kg b.w.) by prediction. The study provided valuable evidence for the application of guvermectin and a better understanding of the biological behavior of nucleoside-like pesticides.
研究领域:
农业科学
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环境科学
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药理学
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毒理学
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植物科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）