CHEMICAL ENGINEERING JOURNAL
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2025
·
IF 13.2
Shumin Zhang,Kexin Zou,Qingmin Chen,Jie Wei,Tianhui Jiao,Min Chen,Yi Xu,Quansheng Chen,Xiaomei Chen
Developing a fast, accurate, and simple detection method for food allergens is imperative to prevent food allergy. In this study, an on–off-on photoelectrochemical (PEC) aptasensor with dual-signal amplification was constructed based on a covalent organic frame (COF) film to detect β-lactoglobulin (BLG) and Ara h1. Specifically, a uniform and stable TFTA COF film was in situ grown as a PEC signifier on the surface of an indium tin oxide (ITO) electrode. BLG recognition triggered enzyme-assisted circular signal amplification to introduce non-conductive SiO 2 -modified DNA (S0) to the platform, effectively quenching the PEC signal for ultra-sensitive detection of BLG. Upon adding Ara h1, SiO 2 -DNA was released from the sensing platform to restore the PEC signal, and hemin was added to form a G-quadruplex/hemin compound for secondary signal amplification. Thus, sensitive detections of BLG and Ara h1 were achieved with low detection limits (18 ng/mL for BLG and 2.6 ng/mL for Ara h1) and wide linear ranges (0.05–5 µg/mL for BLG and 0.01–0.2 µg/mL for Ara h1). The dual-amplification and dual-target-responsive PEC aptasensor provides a new idea to develop PEC analysis methods for multi-targets in food and environmental monitoring.
研究领域:
食品科学
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生物医学工程
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分析化学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2022
·
IF 9.221
Shuo Qi,Anqi Sun,Xiaoze Dong,Yuhan Sun,Minwei Qin,Ning Ding,Yin Zhang,Zhouping Wang
Food allergy is a serious immune allergy reaction, that has attracted public attention all over the world due to the increasing incidence. Ara h1, the most abundant allergenic protein in peanuts, has been identified as a biomarker of peanut allergen. Therefore, a sensitive, efficient method for Ara h1 detection is urgently needed to protect allergy sufferers. In this study, a simple and efficient fluorescent aptasensor was proposed for ultrasensitive and rapid detection of peanut allergen Ara h1. The aptamer against Ara h1 was conjugated onto the surface of high-stable perovskite nanocrystal (PNCs) to serve as the fluorescence-labeled recognition probe as well as the signal probe , while the magnetic nanoparticles Fe 3 O 4 (MNPs) were functionalized with the aptamer complementary DNA (cDNA) as the separation probe. The high-stable PNCs-labeled aptasensor [email protected] gives the combined merits of the extraordinary optical properties of PNCs, the efficient magnetic separation capability of MNPs as well as the high specificity of aptamers. The developed high-stable PNCs-labeled aptasensor exhibited excellent linearity (0.1–100 ng/mL), and low limit of detection (0.04 ng/mL). The aptasensor was successfully applied for the determination of Ara h1 in food samples, implying great potential in the field of allergen assay.
研究领域:
食品科学
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分析化学
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生物技术
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DOI
高影响因子
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
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2016
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IF 1
Alei Zhang,Cong Gao,Kequan Chen,Ce Wei,Pingkai Ouyang
The pH of a microbiological culture is important for both cell growth and chitinase accumulation, but the optimal pH is not normally the same for both. The objective of this study was to investigate the effect of pH on chitinase production by Chitinolyticbacter meiyuanensis strain SYBC-H1 (ATCC BAA-2140) in a mineral medium. The results of batch culture at different pH values showed that the optimum pH for cell growth and chitinase production varied with time, although KOH produced the best results for cell growth and chitinase production, NaOH was chosen because of cost considerations. We designed a three-stage pH control strategy using NaOH as the neutralizing agent. Maximum cell growth (1.07 g dry cell weight/l) and maximum chitinase activity (13.6 U/ml) were observed after culture at 26°C for 72 h in a mineral medium. These values were greater by 129% and 162%, respectively, and the length of time to attain maximum chitinase activity was decreased by 12 h, compared with results from an earlier study (Hao et al., 2011b).
研究领域:
微生物学
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生物技术
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DOI
PubMed
高影响因子
Cell Reports
·
2026
·Vol. 45
·期 2
·
IF 6.9
Yao Wang,Wenxue Zhao,Jiahao Niu,Cuifang Liu,Xiaotian Wang,Weihong Yuan,Shanshan Ai,Wolfgang Baumeister,Guohong Li,Aibin He,Peng Xu,Cheng Li,Yujie Sun
Heterochromatin exerts pivotal functions of silencing specific genes and maintenance of genome stability. However, its formation and maintenance mechanisms remain unclear. Here, we discover that the mitotic regulator NuMA, as a nucleoskeleton protein, is required for constitutive heterochromatin organization at the nucleosome level in interphase. NuMA depletion results in shortened nucleosome repeat length, dispersed nucleosome clutches, increased chromatin accessibility, and disrupted transcription repression of long terminal repeats in heterochromatin regions. Such functions of NuMA rely on its interaction with linker histone H1, which stabilizes H1’s binding to chromatin and facilitates nucleosome stacking, as directly visualized by in situ cryo-ET. Notably, NuMA oligomerizes into quasi-meshwork in the nucleoplasm, providing its organization basis as a nucleoskeleton protein. Collectively, our findings illuminate the concerted effect of nucleoskeleton and linker histone on chromatin compaction at the nucleosome level, unveiling a previously unexplored mechanism by which nucleoskeleton regulates heterochromatin formation and maintenance.
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产品关联
DOI
PubMed
高影响因子
Balkan Medical Journal
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2026
·Vol. 43
·期 4
·183
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IF 4.5
Guangnan Huang,Lei Gao,Shu Fang,Likun Xu,Xiaowei Zhao
Background Epithelial–mesenchymal transition and cancer cell stemness (CSC) are critical processes that driving the invasion and lethality of lung adenocarcinoma (LUAD). The chromatin remodeler ARID4B has been implicated in other cancers; however, its specific role and underlying mechanism in LUAD progression remain unclear. Aims To elucidate the function of ARID4B in LUAD. Study Design This study integrated bioinformatics analysis, in vitro cellular functional assays, and in vivo orthotopic lung cancer models to investigate the oncogenic role of ARID4B . Methods ARID4B expression and its correlation with patient prognosis were analyzed using public databases. ARID4B was silenced using two independent short hairpin RNAs in A549 and H1650 LUAD cell lines, and subsequent changes in invasion, migration, and stemness markers were assessed. Tumor metastasis was evaluated using orthotopic and tail-vein injection mouse models. The regulatory mechanism by which ARID4B controls GPRC5C expression was investigated using dual-luciferase reporter and chromatin immunoprecipitation assays. Results ARID4B was significantly upregulated in LUAD and was associated with poor patient prognosis. Silencing ARID4B in LUAD cells significantly reduced their invasion and migration capabilities (adj. p < 0.01). Furthermore, ARID4B knockdown resulted in downregulation of SNAIL, upregulation of E-cadherin, and impairment of stem-like characteristics, as demonstrated by decreased CSC marker expression and reduced sphere-forming ability (adj. p < 0.05). In the orthotopic model, ARID4B deficiency markedly inhibited tumor metastasis (adj. p < 0.001). Mechanistically, ARID4B transcriptionally activated GPRC5C (adj. p < 0.01). Furthermore, ARID4B knockdown increased histone H1 occupancy at the GPRC5C promoter (adj. p < 0.01), indicating that ARID4B facilitates an open chromatin state. Rescue experiments further confirmed that GPRC5C is required for ARID4B -mediated maintenance of tumor invasiveness and cancer stemness. Conclusion ARID4B promotes LUAD malignancy by transcriptionally activating GPRC5C , thereby driving EMT and cancer stemness.
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DOI
PubMed
高影响因子
BIOTECHNOLOGY LETTERS
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2022
·Vol. 44
·期 4
·623-633
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IF 2.716
Hao Zhi-kui,Li Jian-song,Wang Dan-hua,He Fei,Xue Jing-shi,Yin Liang-hong,Zheng Hua-bao
Objectives Shellfish waste is a primary source for making N -acetyl- d -glucosamine. Thus, establishing a high-efficiency and low-cost bioconversion method to produce N -acetyl- d -glucosamine directly from shellfish waste was promising.
Results A mutant C81 was obtained from Chitinolyticbacter meiyuanensis SYBC-H1 via 60 Co-γ irradiation. This mutant C81 showed the highest chitinase activity of 9.8 U/mL that was 85% higher than the parent strain. The mutant C81 exhibted improved antioxidant activities, including total antioxidant capacity, superoxide radical ability, and hydroxyl radical scavenging ability, compared to that of the parent strain. Four out of nine organic solvents increased the chitinase activity by 1.9%, 6.8%, 11.7%, and 15.8%, corresponding to methylbenzene, n-heptane, petroleum ether, and n-hexane, respectively. The biphase system composed of aqueous and hexane presented a five-fold reduction of cell viability compared to the control. Using a continuous fermentation bioconversion process, 4.2 g/L GlcNAc was produced from crayfish shell powder with a yield of 80% of the chitin content. Conclusions This study demonstrated that the mutant C81 is suitable for converting crayfish shell powder into GlcNAc in an aqueous-organic system.
研究领域:
微生物学
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生物化学
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生物工程
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DOI
PubMed
高影响因子
Frontiers in Physiology
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2017
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IF 4.134
Jiang Jianru,Xu Yili,Lin Xinda
Ovarian development plays an important role in the life history of insects and is crucial for control of the insect population. The metamorphosis of an insect is precisely regulated by the interaction of the juvenile hormone and ecdysone. To understand the role of NlBr and NlKr-h1 in ovary development, we used RNA interference (RNAi) to down-regulate the expression of Broad-Complex (Br) and Krüppel homolog 1 (Kr-h1), two important down-stream transcription factors of juvenile hormone and ecdysone signaling. We further investigated their effects on metamorphosis and ovary development. The results showed that both NlBr and NlKr-h1 are induced by ecdysone. The down-regulation of NlBr and NlKr-h1 alone or together by RNAi is more effective than the topical application of ecdysone on the number of ovarioles, suggesting the necessity of NlBr and NlKr-h1 in determining the number of ovarioles. The ovarian grade was significantly increased/decreased by the topical application of ecdysone and down-regulation of NlBr and NlKr-h1. The pre-oviposition period was also increased. When NlBr and NlKr-h1 were down-regulated together, the ovary grade was not significantly different compared to the control (dsGFP), indicating that the development of the ovary is under the control of both NlBr and NlKr-h1. The interaction between the NlBr and NlKr-h1 on the number of ovarioles and the development of the ovary indicates cross-talk between both juvenile hormone and ecdysone signaling at the transcription level in the brown planthopper. Both genes are nuclear transcription factors and may regulate signaling via down-stream genes. These results would help to both enhance the current understanding of the regulatory mechanism of the interaction between juvenile hormone and ecdysone signaling pathways during ovarian development and to design chemicals to control pests.
研究领域:
昆虫学
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分子生物学
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DOI
PubMed
高影响因子
STAR Protocols
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2022
·
IF 0
Jiwen Yang,Minjie Hu,Yongyu Wang
Summary In human pluripotent stem cells (hPSCs), traditional approaches for gene overexpression have low efficiency and are often laborious. Here, we provide a relatively simple protocol for gene overexpression with the Dox-inducible PiggyBac transposon system. We detail the steps for overexpression of FLI1 and/or YAP in H1 embryonic stem cells (H1 ESCs) as an example. Our protocol can be applied to any gene of interest in a variety of hPSCs. For complete details on the use and execution of this protocol, please refer to Quan et al. (2021) .
研究领域:
干细胞生物学
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分子生物学
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基因工程
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DOI
PubMed
SENSORS AND ACTUATORS B-CHEMICAL
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2023
·
IF 8.4
Wenjing Huang,Li Wang,Hao Zhang,Zhihao Han,Yueqing Gu
The incidence of prostate cancer has increased rapidly in recent years. And the detection of tumor markers is an effective way for early diagnosis and poor prognosis. Prostate cancer is associated with growth factor receptors. Epidermal growth factor receptor (EGFR) expression is increased in a majority of prostate cancer patients. Peptide probes have the advantages of convenient modification and selective and rapid clearance in the molecular imaging of tumors. H1 is a short peptide designed and modified based on the CDR3 region of a nanobody targeting the epidermal growth factor receptor (EGFR) with high affinity for malignant tumors that overexpress EGFR. The CDR3 sequence of nanobodies has a long finger-like structure that allows deep interaction with the cryptic epitope of the antigen. Here we synthesized a sensitive fluorescent peptide probe of H1 conjugated to imaging moieties MPA. Experimental data showed that H1-MPA targets EGFR with high affinity. Meanwhile, H1-MPA has high tumor uptake and a strong signal-to-noise ratio. In addition, the near-infrared fluorescent probe H1-MPA can determine tumor boundaries and can be used to monitor and prevent tumor metastasis, including hepatic metastasis, for accurate tumor diagnosis. H1-MPA provides a promising tool for cancer diagnosis.
研究领域:
肿瘤学
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分子生物学
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生物医学工程
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癌症诊断
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荧光成像
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产品关联
DOI
高影响因子
DYES AND PIGMENTS
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2018
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IF 3.767
Bin Jia,Hong Lian,Tijian Sun,Hongen Guo,Xiaozhe Cheng,Jian Wu,Yi Chen,Qingchen Dong,Jinhai Huang
Two host materials H1 and H2 by introducing dibenzothiophene and dibezofuran moieties into the carbazole and pyridine derivative, respectively, are reported. Their structural and physical properties were fully characterized. The thermal analysis demonstrated that both H1 and H2 are thermally stable. The triplet energy levels of H1 and H2 were determined to be 2.66 and 2.51 eV, respectively. Green phosphorescent OLEDs with H1 and H2 acting as host materials were fabricated to investigate their performances in PHOLEDs . Green PHOLED based on H1 shows the best performance with the maximum power efficiency of 50.4 lm/W and the lowest driving voltage of 2.5 eV. The characteristic measurements of OLED devices reveal that both H1 and H2 are promising host materials for practical application. By comparing with the commercial host material of 4,4′-bis(carbazol-9-yl)biphenyl (CBP), the power efficiency of green PHOLED was enhanced by 31.6%.
研究领域:
材料科学
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有机电子学
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光电材料
✓ 已验证
DOI
高影响因子
Cell Reports
·
2024
·
IF 7.5
Sijia Ren,Feng Bai,Viviane Schnell,Clara Stanko,Muriel Ritsch,Tino Schenk,Emanuel Barth,Manja Marz,Bin Wang,Xin-Hai Pei,Holger Bierhoff
Extensive remodeling of the female mammary epithelium during development and pregnancy has been linked to cancer susceptibility. The faithful response of mammary epithelial cells (MECs) to hormone signaling is key to avoiding breast cancer development. Here, we show that lactogenic differentiation of murine MECs requires silencing of genes encoding ribosomal RNA (rRNA) by the antisense transcript PAPAS. Accordingly, knockdown of PAPAS derepresses rRNA genes, attenuates the response to lactogenic hormones, and induces malignant transformation. Restoring PAPAS levels in breast cancer cells reduces tumorigenicity and lung invasion and activates many interferon-regulated genes previously linked to metastasis suppression. Mechanistically, PAPAS transcription depends on R-loop formation at the 3' end of rRNA genes, which is repressed by RNase H1 and replication protein A (RPA) overexpression in breast cancer cells. Depletion of PAPAS and upregulation of RNase H1 and RPA in human breast cancer underpin the clinical relevance of our findings.
研究领域:
癌症生物学
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分子生物学
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细胞生物学
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DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 16
·6063
·
IF 4.6
Yali Guo,Bing Wang,Lifei Gu,Guo Yin,Shuhong Wang,Meifang Li,Lijun Wang,Xie-An Yu,Tiejie Wang
The fraud phenomenon is currently widespread in the traditional Chinese medicine Radix Astragali (RA) market, especially where high-quality RA is substituted with low-quality RA. In this case, focused on polysaccharides from RA, the classification models were established for discrimination of RA from different growth patterns, origins, species, and growth years. 1H Nuclear Magnetic Resonance (H1-NMR) was used to establish the spectroscopy of polysaccharides from RA, which were used to distinguish RA via chemical pattern recognition methods. Specifically, orthogonal partial least squares discriminant analysis (OPLS-DA) and linear discriminant analysis (LDA) were used to successfully establish the classification models for RA from different growth patterns, origins, species, and growth years. The satisfactory parameters and high accuracy of internal and external verification of each model exhibited the reliable and good prediction ability of the developed models. In addition, the polysaccharide content and immunological activity were also tested, which was evaluated by the phagocytic activity of RAW 264.7. And the result showed that growth patterns and origins significantly affected the quality of RA. However, there was no significant difference in the aspects of origins and growth years. Accordingly, the developed strategy combined with chemical information, biological activity, and multivariate statistical method can provide new insight for the quality evaluation of traditional Chinese medicine.Keywords:Radix Astragali;immunomodulatory activity;polysaccharide content;chemical pattern recognition;quality evaluation
研究领域:
中药学
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生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）