A Novel H2O2 Biosensor Based On the composite of MP-11 encapasulated in PCN-333(Al)-Graphene Oxide
In this work, a novel H 2 O 2 biosensor was developed based on the composites of microperoxidase-11 (MP-11) encapsulated in graphene oxide (GO)-PCN-333 (Al) (PCN stands for porous coordination network, one of the metal-organic frameworks). The PCN-333(Al) firmly and uniformly grew on the surface of GO sheet with large specific surface and excellent electrical conductivity, which provided a lots of pores for the encapsulation of MP-11 in the composites and selectively accumulated analytes into its mesopores to improve the selectivity of enzymatic reaction. The MP-11 molecules might freely move through mesopores of PCN-333 (Al) to maintain bioactivity, which remarkably enhanced the mass transfer rate and quickened the electron transfer for electrochemical biosensors. Scanning electron microscopy was used to characterize the GO and PCN-333(Al)-GO composites. Cyclic voltammetry and differential pulse voltammetry were used to characterize the electrochemical behaviors and performance of the H 2 O 2 biosensor. The target electrode showed excellent performance with a wide linear range from 10 μ M to 800 μ M, a low detection limit of 3 μ M, good stability and high selectivity, which was superior to some other H 2 O 2 biosensors.
危险品化学品经营许可证（带存储）