Journal of Biomedical Nanotechnology
·
2018
·Vol. 14
·期 6
·1169-1177
·
IF 5.068
Ma Yuchan,Lu Mingyue,Deng Yan,Bai Ruyan,Zhang Xi,Li Delei,Zhang Kunlei,Hu Rong,Yang Yunhui
In this work, a nano-mimetic enzyme Co3O4 nanoparticles with catalytic effect on the reduction of H2O2, was synthesized and used to label C-reactive protein antibody. Au nanoparticles (Au NPs) were loaded on COF-TpPa-1 to form Au NPs@COF-TpPa-1
with good biocompatibility and adsorption ability. A sandwich C-reactive protein immunosensor was prepared using Au NPs @COF-TpPa-1 as the immobilization matrix. The catalytic current of Co3O4 nanoparticles to the reduction of H2O2 was measured by
the chronoamperometry which is in proportion to the concentration of CRP. The linear range of this immunosensor was 0.05–80 ng/mL with the linear correlation coefficient of 0.9955. The method demonstrated a good sensitivity with a detection limit of 0.017 ng/mL (S/N = 3) for CRP.
When the C-reactive protein immunosensor was applied to real serum samples analysis, the satisfied results were obtained, indicating that the immunosensor is of prospect of application.
研究领域:
生物医学工程
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2025
·
IF 5.6
Juan Li,Jinggao Wu,Chang Ming Li
Dual hetero paired single atomic catalyst (DHAC, CoCu@CN) was one-pot synthesized by a facile green method and applied as a nanozyme for the detection of H 2 O 2 . Both experimental results and theoretical calculations discover that the hetero-paired DAC realizes stronger adsorption energy and a bridge adsorption of H 2 O 2 for simultaneous 2-electron transfer, thereby offering much higher electrocatalytic activity and larger catalytic current than that of single Co atom and single Cu catalyst alone toward H 2 O 2 reduction. The CoCu@CN H 2 O 2 sensor demonstrates a wide linear detection range (0.654–7379.454 μM) and low limit of detection (LOD) of H 2 O 2 (0.047 μM), and is used successfully to in-site detect intracellular H 2 O 2 released from living cells, ensuring its practical applications in life science and medical diagnosis. This work not only discloses a scientific insight for the enhancement mechanism of the dual single heteroatomic catalyst, but also demonstrates an avenue to synthesize more efficient dual single heteroatomic catalyst for potential broad applications in energy conversion and/or sensing applications.
研究领域:
生物化学
·
材料科学
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2019
·Vol. 144
·期 23
·6968-6974
·
IF 4.019
Jianqiao Liu,Li Quan,Xuelian Yu,Lin Wang
In this work, a sandwich-type electrochemical immunosensor for the detection of procalcitonin (PCT) is constructed layer-by-layer with a novel label based on MoO3/Au@rGO nanocomposites. The high surface area and electroconductivity of graphene contributes to the transfer of electrons. Besides, the introduction of Au modulates the morphology of the MoO3@rGO nanocomposites from hexagonal prisms to agglomerated particles. Compared to rGO, Au@rGO and MoO3@rGO, the synergistic effect of MoO3/Au@rGO nanocomposites leads to the highest electrocatalytic activity towards H2O2 reduction. The electrochemical immunosensor exhibits a wide working range from 0.01 pg mL−1 to 10 ng mL−1 and a detection limit of 0.002 pg mL−1 at a signal-to-noise ratio of 3. Additionally, we also obtain excellent sensing performance for the immunosensor, including excellent selectivity, good reproducibility and stability. This work will give insight into the application of the nanocomposites based on noble metals combined with transition metal oxides in the fields of electroanalytical chemistry and biosensors.
研究领域:
分析化学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2023
·
IF 6.7
Chengcheng Qi,Yuhua Luo,Yongping Dong
The study of single atoms in the field of electrochemical sensing is at the early stage. How to boost the catalytic performance of single atoms is still a big challenge. Herein, we prepare Fe-Se dual single-atom sites on N-doped ultrathin carbon carrier (Fe 1 Se 1 /NC) for realizing highly sensitive nonenzymatic detection of H 2 O 2 . Fe 1 Se 1 /NC was synthesized through the pyrolysis strategy, and then was characterized by transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS) mapping, high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM), X-ray diffraction (XRD), X-ray photoelectron spectrometer (XPS), and electrochemical techniques, respectively. The results displayed that Fe and Se both existed in an atomically dispersed status. Compared to the single-atom Se or Fe catalyst (Se 1 /NC or Fe 1 /NC), the Fe 1 Se 1 /NC significantly boosted the electrocatalytic activity for H 2 O 2 reduction. The Fe 1 Se 1 /NC modified electrode could be used to detect H 2 O 2 in a wide linear range of 0.02 mM to 13 mM with a high sensitivity of 1508.6 µA·mM −1 ·cm −2 and a low detection limit of 11.5 µM. Moreover, the sensor was successfully employed for detecting H 2 O 2 in disinfectant and urine samples. This work provides a novel design of dual-atom catalysts for excellent electrochemical sensing applications.
研究领域:
电化学
·
催化
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2020
·
IF 3.99
Qiaoxia Zhang,Weikang Li,Fengyang Zhao,Chang Xu,Gaochao Fan,Qingyun Liu,Xianxi Zhang,Xiao Zhang
An ultrasensitive enzyme-free electrochemical sandwich-type thrombin aptasensor is proposed in this work, with the amplifier of Pt loaded hollow zinc ferrite nanospheres . Silver nanowires& particles (AgNWs&PCs) are used as the substrate materials to decorate the working electrode . Silver particles (AgPCs) are introduced for welding of AgNWs to further improve the electrical performance of the working electrode. The conductive electrode decorated with AgNWs&PCs can provide a substrate for the immobilization of thrombin aptamer 1 (Apt1). Moreover, AgNWs&PCs possess the catalytic activity for H 2 O 2 reduction, contributing to the signal amplification. The prepared Pt loaded hollow zinc ferrite nanospheres with an excellent electrocatalytic activity for H 2 O 2 reduction can also be used to immobilize aptamer 2 (Apt2). Under the optimized condition, the performance of the biosensor is evaluated by amperometry . The proposed thrombin aptasensor has a wide linear detection range from 0.05 pM to 35 nM and a low detection limit of 0.016 pM (S/N = 3), due to the synergistic effect of AgNWs&PCs electrode and the Pt loaded hollow zinc ferrite nanospheres amplifier. This aptasensor displays high sensitivity, good reproducibility and stability, providing a promising platform for the analysis of thrombin.
研究领域:
分析化学
·
生物传感器
·
纳米技术
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）