Zhonghua Xue,Xiaofen Wang,Honghong Rao,Xiuhui Liu,Xiaoquan Lu
Many strategies have been explored for selectively and sensitively detecting cysteine in different samples. Here, a novel colorimetric sensor based on self-assembly nanostructures of Fe 3+ -H 2 O 2 /Tetramethylbenzidine system with dual-level logic gate function and colorimetric determination of cysteine were firstly explored. The proposed Fe 3+ -H 2 O 2 -TMB system provides a sensitive optical signal due to the selectively reductive ability of cysteine to the oxidized TMB and thus could be successfully applied to the construction of instant on-site visual detection method with a paper based test strip for cysteine determination in a sample solution as well as for a dual-level logic gate fabrication.
A colorimetric assay based on an enzyme-inhibition strategy is promising for the on-site detection of pesticide residues . However, very few works of pesticide detection were reported based on the inhibition toward nanozymes although nanozymes have demonstrated many advantages in sensing various targets. Herein, a facile colorimetric detection for Glyp was developed based on β [email protected] enzyme mimics. The β [email protected] with high peroxidase-like activity was synthesized using random DNA double strands as template and β -CD as surface ligand. β [email protected] could catalyze the H 2 O 2 -3,3′,5,5′-tetramethylbenzidine (TMB) system. The oxidation product OxTMB with a blue color and presented a large absorption signal at 652 nm. However, Glyp could destroy the synergic effect between redox doublet (Cu 2+ /Cu + ) on the β [email protected] A-CuNCs surface, resulting in the inhibition of the peroxidase-like activity. Colorimetric detection for Glyp could be established by detecting the changes of absorption signal at 652 nm. The linear range was 0.02–2 μ g/mL and the detection limit was 0.85 ng/mL (3δ/s). The method was applied in measuring Glyp spiked in lake water and various food samples. This method had rapidness, high sensitivity, and selectivity advantages, indicating the high application potential in monitoring Glyp residue in food.
Lei Wang,Ai Xu,Jing Yuan,Fan Jiang,Meixuan Li,Wuzhen Qi,Yanbin Li,Jianhan Lin
An hourglass-mimicking biosensor was developed to detect target bacteria in 15 mL centrifugal tube using immune magnetic nanobeads to isolate the target from large-volume sample, [email protected] nanozymes to label the target for amplification of biological signals, and a microplate reader/colorimetric card for determination of the target. First, a centrifugal tube with an iron ball framework was first coaxially assembled in the center of a Halbach ring magnet. After immune magnetic nanobeads, [email protected] nanozymes and bacterial sample were mixed by repeated bottom-up of the tube using a stepper motor , nanobead-bacteria-nanozyme complexes were formed. Then, colorless H 2 O 2 -TMB was catalyzed by the nanozymes to produce blue TMBox. The color change was finally analyzed using the microplate reader or colorimetric card to determine bacterial concentration . This hourglass-mimicking biosensor could separate ∼95% targets from 10 mL bacteria sample and detect targets from 1.6 × 10 1 to 1.6 × 10 6 CFU/mL in 1.0 h with low detection limit of 16 CFU/mL.