ULTRASONICS SONOCHEMISTRY
·
2024
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IF 8.7
Yunxiang Que,Yao Zhang,Fengxiang Liang,Liping Wang,Yiting Yang,Jingbo Zhang,Wanting Wang,Ying Sun,Changjiao Zhong,Haipeng Zhang,Chengguang He,Lili Guan,Hongxia Ma
Adhere to the concept of low-carbon environmental protection and turning waste into treasure, polysaccharides from Flammulina velutipes residue polysaccharide (FVRP) has been developed and possesses diverse bioactivities, comprising antioxidant, hypoglycemic, and relieving heavy metal damage, which still has the disadvantages of high molecular weight and low bioavailability. The current work is the first to prepare a degraded polysaccharide (FVRPV) from FVRP by ultrasonic assisted H 2 O 2 -Vc technique in order to reduce its molecular weight, thereby improving its activity and bioavailability. Our results found that the molecular weight and average particle size were declined, but the monosaccharide composition and characteristic functional group types of FVRPV had no impact. The structural changes of polysaccharides analyzed by XRD, Congo Red test, I 2 -KI, SEM, and methylation analysis indicated that the surface morphology and glycosidic bond composition of FVRPV possessed longer side chains and a greater number of branches with an amorphous crystal structure devoid of a triple helix configuration, and had experienced notable alterations after ultrasonic assisted H 2 O 2 -Vc treatment. Meanwhile, the in vitro antioxidant capacity of FVRPV had significantly increased compared to FVRP, implying ultrasonic assisted H 2 O 2 -Vc technique maybe a effective method to enhance the bioactivity of polysaccharides. In addition, the content of polysaccharide, reducing sugar, and uronic acid in FVRPV was significantly decreased, but antioxidant capacity of fermentation broth was stronger by in vitro human fecal fermentation. The 16S rDNA sequencing data displayed that FVRPV can enrich probiotics and reduce the abundance of pathogenic bacteria through different metabolic pathways mediated by gut microbiota, thereby exerting its potential probiotic effects. The interesting work provides a novel degraded polysaccharide by ultrasonic assisted H 2 O 2 -Vc technique, laying a foundation for developing FVRPV as a new antioxidant and prebiotic.
研究领域:
食品科学
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生物技术
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生物化学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Wudan Cai,Shuai Chen,Xiao Zhang,Dan Wu,Xinyi Zhu,Honglin Zhu,Yangchao Luo,Qilin Huang
The current study aimed to investigate the effect of ultrasound assisted H 2 O 2 /Vc degradation on the digestive characteristics of Lignosus rhinocerotis polysaccharide (LRP) and gut microbiota regulation. Results showed that during in vitro digestion, no changes were observed in molecular weight ( M w ) of LRP and its antitumor activity remains stable. However, M w of degraded LRP (DLRP) slightly decreased from 45.66 × 10 4 Da to 36.61 × 10 4 Da with no free monosaccharides released, enhancing its antitumor activity. The supplementation with LRP or DLRP significantly elevated mice fecal water content and modulated microbiota composition. The total short chain fatty acids concentration of control, LRP, and DLRP groups were 20.07, 24.13, and 28.08 mmol/L, respectively. Compared with LRP, DLRP significantly increased the abundance of beneficial bacteria such as Alloprevotella and Mucispirillum , exhibiting protective effects on intestinal health. This study provides a theoretical foundation for targeted regulation of intestinal flora by LRP and DLRP.
研究领域:
食品科学
·
微生物学
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药理学
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Huadan Zhang,Congcong Sun,Kailin Li,Xianliang Luo,Zebin Guo,Song Miao,Baodong Zheng
Fucoidan, a marine-derived sulfated polysaccharide, possesses a diverse range of bioactive properties. This study aimed to evaluate the physicochemical characteristics and anti-inflammatory activity of Laminaria japonica fucoidan (LJF) and degradation fractions (DLJF-Ts) obtained through ultrasonic-assisted H 2 O 2 /Vitamin C (VC) treatment (1, 3, and 6 h). The molecular weights of LJF, DLJF-1, DLJF-3, and DLJF-6 were 225, 101, 45, and 31 kDa, respectively. The fucose was the major monosaccharide, and structural analysis demonstrated that the main functional groups were unchanged, while their sheets changed from larger to smaller. Meanwhile, the DLJF-3 showed the superior effects of in vitro antioxidant and anti-inflammatory. Furthermore, after 48 h of colitis mice fecal fermentation, the DLJF-3 was metabolized by gut microbiota, leading to a marked elevation in short-chain fatty acids. The content of Bacteroides increased and the Desulfovibrio reduced significantly. Overall, the obtained results revealed that DLJF-3, as the anti-inflammatory agent, could be used for the development of functional foods and healthcare products.
研究领域:
海洋生物技术
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食品科学
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药理学
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免疫学
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
Food Chemistry-X
·
2022
·
IF 6.443
Xiaoshan Long,Xiao Hu,Huan Xiang,Shengjun Chen,Laihao Li,Bo Qi,Chunsheng Li,Shucheng Liu,Xianqing Yang
This research aimed to analyze structural characterization and hypolipidemic activity in vitro of G. lemaneiformis polysaccharide (GLP) and its degradation products. The results presented that the content of galacturonic acid declined and glucuronic acid level enhanced, average particle size decreased from 99.9 μm to 25.7 μm, and color brightness of polysaccharide strengthened after degraded by H 2 O 2 -Vc. There was no significant change in thermal stability of polysaccharide before and after degradation. It was observed in AFM analysis, polysaccharide changed to smaller, delicacy and dispersion after degradation. As seen in FT-IR, H 2 O 2 -Vc degradation never change the structure of polysaccharide. Polysaccharide and its degradation products showed a significant inhibition effect on pancreatic lipase and cholesterol esterase in a dose-dependent manner, which presented the mixed type of competitive and non-competitive for pancreatic lipase, and non-competitive for cholesterol esterase, respectively. The fluorescence quenching type was static on pancreatic lipase and dynamic on cholesterol esterase.
研究领域:
海洋生物学
·
生物化学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）