Journal of Biomaterials and Tissue Engineering
·
2023
·Vol. 13
·期 6
·753-764
·
IF 0.1
Cheng Xu-Mei,Ma Rui,Zhou Yan-Bing,Li Tao,Lei Sheng-Nan,Zheng Xin,Zhang Hui-Xiao,Liu Mei-Tian,Zhou Jian-Ye,Li Zhi-Qiang
Bone defect repair is a hot topic in tissue engineering, and the development of economical and effective bone scaffold materials to achieve the goal of repairing bone defects is an important task for researchers. In this experiment, fresh bovine cancellous bone was used as a xenogeneic
bone material. After two high-temperature calcinations, β-tricalcium phosphate (β-TCP) material was obtained. An in-situ co-precipitation method was employed to prepare hydroxyapatite+graphene oxide (HA+GO) composite material. Then, the β-TCP material
was immersed in the HA+GO composite material solution to prepare β-TCP+HA+GO composite material. The surface modification of β-TCP material with HA and GO improved the elastic modulus of the raw material. In vitro cell showed that the composite material was non-toxic,
biocompatible, and promoted the proliferation of MC3T3-E1 cells on the β-TCP+HA+GO material. Finally, it was found that β-TCP+HA+GO composite material effectively stimulated the actin and mineralization behavior of MC3T3-E1 cells, and promoted the expression of bone-related
factors such as Alkaline phosphatase (ALP), Collagen I (COLI), Osteocalcin (OCN), Bone Morphogenetic Protein-2 (BMP-2), and Bone Morphogenetic Protein-7 (BMP-7) in MC3T3-E1 cells In conclusion, β-TCP+HA+GO composite material can be applied in the biomedical field as a bone substitute
or repair material.
研究领域:
组织工程学
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生物医学材料
·
细胞生物学
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DOI
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2023
·
IF 10.8
Chang Ni,Zijun Zhang,Yuling Wang,Zhenhai Zhang,Xueping Guo,Huixia Lv
Hyaluronic acid (HA) has been widely used in cosmetics and topical preparations owing to its favorable moisturizing property and potential in enhancing drugs' skin permeability. Here, the influencing factors and underlying mechanism of HA on skin penetration were carefully investigated, and HA-modified Undecylenoyl-Phenylalanine (UP) liposomes (HA-UP-LPs) were designed as a proof of principle for efficacious transdermal drug delivery strategy to enhance the skin penetration and retention. An in vitro penetration test (IVPT) of HA with different molecular weights showed that low molecular weight HA (LMW-HA, 5 kDa and 8 kDa) could pass through the stratum corneum (SC) barrier and enter into the epidermis and dermis layers, whereas its high molecular counterparts (HMW-HA) were trapped on the SC surface. Mechanistic studies revealed that LMW-HA could interact with keratin and lipid in the SC meanwhile exerted a substantial skin hydration effect, which may partially contribute to the SC penetration benefit. In addition, the surface decoration of HA drove an energy-dependent caveolae/lipid raft-mediated endocytosis of the liposomes through direct binding to the CD44 receptors widely expressed on skin cell membranes . Notably, IVPT showed a 1.36-fold and 4.86-fold increase in skin retention of UP and a 1.62-fold and 5.41-fold increase in skin penetration of UP with HA-UP-LPs over UP-LPs and free UP at 24 h, respectively. As a result, the anionic HA-UP-LPs (−30.0 mV) showed enhanced drug skin penetration and retention compared with conventional cationic bared UP-LPs (+21.3 mV) on both in vitro mini-pig skin as well as in vivo mouse skin. Overall, the usage of LMW-HA might offer opportunities in developing novel topical preparations and skin care products with improved transdermal penetration and retention.
研究领域:
药剂学
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化妆品科学
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药物递送系统
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生物药剂学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
·
2024
·Vol. 99
·期 7
·1637-1645
·
IF 2.8
Wei Wu,Xiaoqian Ma,Rui Wang,Dayong Zhang
BACKGROUND Demulsification using chitosan (CS)-coated magnetic nanomaterials has garnered significant attention. However, traditional methods for synthesizing chitosan (CS)-coated magnetic nanomaterials are often contaminated, laborious, and time-consuming. Moreover, they showed poor demulsification efficiency due to the occupation of free amino groups in CS by crosslinking agents. RESULTS CS-coated Fe 3 O 4 magnetic nanoparticles (MNPs) were synthesized using humic acid (HA) as a bridge. The Fe 3 O 4 /HA/CS MNPs are recyclable demulsifiers for hexadecane-water microemulsions. The impacts of MNP dosage, separation time, and pH on demulsification efficiency were studied comprehensively. Compared with Fe 3 O 4 /HA and Fe 3 O 4 /CS MNPs, the demulsification efficiency of the Fe 3 O 4 /HA/CS MNPs was superior. The pseudo-second-order kinetic model and the Sips isotherm best describe the demulsification process. Combined with the influence factors of demulsification efficiency, the demulsification mechanism involves electrostatic interactions between MNPs and oil microparticles. The maximum adsorption capacity for oil by the Fe 3 O 4 /HA/CS MNPs was 91.73 mg g −1 . CONCLUSION This research presents a new method for connecting CS to MNPs with a high surface positive charge. As synthesized Fe 3 O 4 /HA/CS MNPs is expected to serve as highly efficient and recyclable demulsifiers for emulsified oil wastewater. © 2024 Society of Chemical Industry (SCI).
研究领域:
材料科学
·
环境科学
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产品关联
DOI
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2023
·
IF 5.2
Pai Peng,Yaning Yue,Mengxi Han,Jun Shi,Jing Yang,Junwen Chen,Qiang Chen
Design of heterogeneous catalyst to achieve efficient conversion of biomass to high added-value chemicals is still very attractive. Herein, taking industrially important selective hydrogenation of furfural to furfuryl alcohol as an example, the designed amorphized HA zeolite encapsulation of platinum clusters (denoted as Pt@HA) exhibited excellent catalytic selectivity at totally conversion of the reactant furfural, along with good reusability and poison resistance ability. The selected amorphized HA zeolite with very small pore size constrained the reactant furfural to be reacted through hydrogen spillover process at zeolite surface, instead of to be adsorbed and activated onto Pt surface. Resulting from the stronger interaction between nucleophilic oxygen atom of aldehyde group and acid site at HA zeolite surface than that of furan ring group, furfural preferred to adsorb onto Pt@HA surface with the end of aldehyde group rather than the end of furan ring group, thus facilitating the spilled H atom to react with aldehyde group to give desired furfuryl alcohol product exclusively.
研究领域:
催化化学
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生物质转化
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2023
·
IF 5.8
Ziwei Liang,Yuhui Wang,Jiapu Wang,Tao Xu,Shilong Ma,Qi Liu,Liqin Zhao,Yan Wei,Xiaojie Lian,Di Huang
Ferroptosis is a novel form of regulated cell death induced by iron-dependent lipid peroxidation imbalance. It has emerged as a promising antitumor therapeutic strategy in recent years. In this work, we successfully synthesized a complex magnetic nanocube Fe 3 O 4 modified with PEI and HA by the thermal decomposition method. While loading a ferroptosis inducer RSL3 inhibited cancer cells through the ferroptosis signal transduction pathway. The drug delivery system could actively target tumor cells through an external magnetic field and HA-CD44 binding. Zeta potential analysis showed that Fe 3 O 4 [email protected] nanoparticles were more stable and uniformly dispersed in tumor acidic environment. Moreover, cellular experiments demonstrated that Fe 3 O 4 [email protected] nanoparticles could significantly inhibit the proliferation of hepatoma cells without a cytotoxic effect on normal hepatic cells. In addition, Fe 3 O 4 [email protected] played a vital role in ferroptosis by accelerating ROS production. The expression of ferroptosis-related genes Lactoferrin, FACL 4, GPX 4 and Ferritin was significantly suppressed with increasing treatment of Fe 3 O 4 [email protected] nanocubes. Therefore, this ferroptosis nanomaterial has great potential in Hepatocellular carcinoma (HCC) therapy.
研究领域:
纳米技术
·
癌症生物学
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药学
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分子生物学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 3.8
Sisi Li,Shuang Li,Xiang Li,Xiaodan Zeng,Jie Chen,Zhigang Liu,Shihua Yu,Junjing Hao
Humic acid (HA) is a naturally biomass, that is considered as an ideal adsorbent material due to the abundance of functional groups on its surface. For this purpose, the highly dispersible magnetic humic acid (Fe 3 O 4 @HA) has been prepared by one-step solvothermal method. The developed Fe 3 O 4 @HA not only has a strong separation, but also can simultaneously adsorb tetracycline antibiotics (tetracycline, chlortetracycline hydrochloride and doxycycline hydrochloride). Batch sorption experiments convinced that the maximum tetracycline antibiotics adsorption capacity of Fe 3 O 4 @HA is 45.58, 86.87, and 88.46 mg/g and the largest removal of 94.5 %, 96.3 %, and 97.4 %, fast equilibrium time of 60 min. Meanwhile, the adsorption process is agreement with pseudo-second-order adsorption kinetics and Langmuir adsorption isotherm model. Thermodynamic data indicate that this adsorption is endothermic and spontaneous. Moreover, the designed Fe 3 O 4 @HA with the characteristic of anti-interference capabilities, a variety of water-matrix suitability and maintaining at least 80 % tetracycline antibiotics removal after five adsorption cycles. Finally, the adsorption mechanisms are explored by XPS and FT-IR analysis, which show that mainly involve electrostatic adsorption; hydrogen bonding, π–π, oxygen-containing functional group interactions; and physical adsorption on the surface. In conclusion, Fe 3 O 4 @HA demonstrates considerable promise as an adsorbent for treating antibiotic-contaminated water.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2022
·
IF 6.064
Song Xianji,Liu Xilin,Ma Yihang,Zhu Qingsan,Bi Mingchao
It is important for future clinical applications to design and synthesize multipurpose scaffolding materials for bone tissue engineering with high osteogenic induction and MRI capability. In the present study, we synthesized Ce/Gd@HA by co-doping Ce3+ and Gd3+ into hydroxyapatite (HA) using a hydrothermal synthesis method, and then Ce/Gd@HA composites were synthesized by combining Ce/Gd@HA nanoparticles with polylactic-co-glycolic acid (PLGA) to investigate whether implanted Ce/Gd@HA/PLGA composites could promote osteoblast viability, leading to tibia repair of the rats and enhance MRI. The measurement results contain X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, and environmental scanning electron microscopy (ESEM) showing that HA doped with Ce3+ and Gd3+ was still a hexagonal crystal with high crystallinity. The synthesized Ce/Gd@HA/PLGA composites have a structure and obvious magnetic resonance imaging (MRI) capability. The in vitro experimental results indicated that Ce/Gd@HA/PLGA composites significantly promoted the performance of MC3T3-E1 cells, containing proliferation, adhesion, and osteogenic differentiation capacities. These include the improvement of alkaline phosphatase activity, enhancement of mineral deposition, and upregulation of OCN and COL-1 gene expression. The in vivo experimental results demonstrated that the Ce/Gd@HA/PLGA composites significantly improved the healing rate of rat bone defects. The MRI images indicated that the Ga-doped composites were observed in the MRI T1 sequence in rats. The aforementioned results suggested that Ce/Gd@HA/PLGA composites not only effectively promoted bone formation but also enhanced MRI capability. The composites synthesized in this study have great potential in bone regeneration with an extensive application in bone tissue engineering.
研究领域:
生物材料学
·
组织工程学
·
医学影像学
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DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2024
·
IF 5.1
Xin Zhao,Jimin Chen,Yong Zeng
BaTiO 3 /Ca 10 (PO 4 ) 6 (OH) 2 composite ceramic is an outstanding representative of piezoelectric biomaterials, with excellent biocompatibility and piezoelectric effect, and has potential applications in the field of bone tissue repair. In this work, vat photopolymerization 3D printing technology was used to fabricate triply periodic minimal surface structure BaTiO 3 /Ca 10 (PO 4 ) 6 (OH) 2 composite ceramic bone tissue scaffolds with different pore sizes and porosity, and their mechanical and electrical properties were studied. First, the ceramic slurry configuration process was optimized to obtain a ceramic slurry with high solid content (45 vol%) and excellent rheological properties. Then the effect of sintering temperature on microstructure, relative density, mechanical properties, and electrical properties is discussed. The results show that when sintering at 1300 °C, the BaTiO 3 /Ca 10 (PO 4 ) 6 (OH) 2 composite ceramic has the highest relative density (99.18 %), the highest compressive strength (44 MPa), large relative dielectric constant (379–389), and low dielectric loss. The polarization electric field strength of the BaTiO 3 /Ca 10 (PO 4 ) 6 (OH) 2 composite ceramic was set to 15 kV/cm through the test of the hysteresis loop. Finally, based on multi-physics coupled finite element simulation, the effects of different porosity and different pore sizes on stress distribution and piezoelectric potential were analyzed, and the relationship between them was explored through experiments. The results show that as the porosity increases and the pore size decreases, the mechanical properties of the scaffold decrease significantly, and its compressive strength ranges between 1.67 and 4.26 MPa; as the porosity increases and the pore size increases, the piezoelectric coefficient ( d 33 ) of the scaffold showed a decreasing trend, and its d 33 ranged between 2 and 9 pC/N. The mechanical and electrical properties of the scaffold meet the performance requirements of cancellous bone. In summary, this work provides a strategy for the application of customized BaTiO 3 /Ca 10 (PO 4 ) 6 (OH) 2 composite ceramic scaffolds in new-generation orthopedic implants.
研究领域:
生物材料学
·
骨科医学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2020
·
IF 2.69
Mengjie Xu,Miao Qin,Xiumei Zhang,Xiaoyu Zhang,Jingxuan Li,Yinchun Hu,Weiyi Chen,Di Huang
In the present work, a new kind of porous polyvinyl alcohol/sodium alginate/hydroxyapatite (PVA/SA/HA) composite hydrogels with tunable structure and mechanical properties have been fabricated by dual-crosslinking method. The morphologies, moisture content, porosity and mechanical properties of the composite hydrogels have been investigated in detail. The PVA/SA/HA hydrogels present uniform, interpenetrating porous structure. The FTIR and XRD results indicate that PVA, SA and HA could be uniformly compounded. The mechanical properties, moisture content and porosity of the samples could be controlled by changing the mass ratio of PVA/SA/HA. The optimized compression modulus (41.74 ± 7.86 kPa), moisture content (86.99 ± 0.72%) and porosity (79.98 ± 1.61%) of the composite hydrogels could be achieved via fixing the weight ratio of PVA/SA/HA at 42:18:40. In vitro biodegradation and mineralization of the composite hydrogels show that the hydrogels could gradually be degraded in PBS solution and sheet-like HA nanocrystals are easily formed on the surface. Moreover, cell culture results indicate that the PVA/SA/HA hydrogels have no negative effects on MC3T3-E1 cell growth and proliferation. Alkaline phosphatase (ALP) activity expressions demonstrate that the nano-HA crystals incorporated composite hydrogels obviously improve the ALP activity of the cells. It confirms that the prepared PVA/SA/HA hydrogels could be a promising candidate for bone repair and bone tissue engineering.
研究领域:
生物材料学
·
组织工程学
✓ 已验证
DOI
PubMed
高影响因子
Molecular Catalysis
·
2023
·
IF 4.6
Yaozong Tan,Mengxi Han,Pai Peng,Zongyu Sun,Jun Shi,Yong Huang,Junwen Chen,Licheng Bai,Jing Yang,Qiang Chen
Hydrogen spillover has been widely used in heterogeneous catalysis, yet how to explore the potential of catalyst support to further promote catalytic function of hydrogen spillover has rarely been investigated. Herein, platinum encapsulated into amorphized HA zeolite (denoted as Pt@HA) with generated hydroxyl groups was used as model catalyst to study the role of HA zeolite support on the promotion of catalytic performance in hydrogenation of quinolines to tetrahydroquinolines. Benefiting from small pore size of amorphized HA zeolite to prevent direct interaction of quinoline with encapsulated platinum, Pt@HA showed good catalytic selectivity in this transformation through hydrogen spillover. This remarkable catalytic selectivity was resulted from the interaction of hydroxyl groups at surface of HA zeolite with pyridine moiety through the formation N H bond to facilitate adsorption to make much lower apparent activation energy of Pt@HA for hydrogenation of pyridine moiety than that of benzene moiety, thus exhibiting enhanced catalytic selectivity.
研究领域:
催化
·
化学工程
·
材料科学
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产品关联
DOI
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2019
·Vol. 35
·期 3
·514-520
·
IF 1.071
Liu Guomin,Li Yalong,Yang Shuting,Zhao Yi’an,Lu Tiancheng,Jia Wenyuan,Ji Xuan,Luo Yungang
To explore the ability of dihydroxyphenylalanine-insulin-like growth factor-1(DOPA-IGF-1) coated hydroxyapatite/poly(lactic-co-glycolic acid)(HA/PLGA) microspheres to promote the proliferation and osteoclastic differentiation of rabbit bone mesenchymal stem cells(rBMSCs), HA/PLGA microspheres with different HA content (10%, 30%, 50%, mass fraction) were prepared by electrospinning method and HA/PLGA microspheres with 50% HA were coated with IGF-1 and DOPA-IGF-1, respectively. They were co-cultured with rBMSCs, respctively. Cell counting kit-8(CCK-8) detection, confocal laser scanning microscopy(CLSM), alkaline phosphatase(ALP) detection and osteogenesis related genes COL1A1 , Runx2 and bone morphogenetic protein-2( BMP-2 ) detection were conducted to detect the proliferation activity, cell morphology, differentiation ability and the expression level of osteogenesis-related genes of cells cultured on all microspheres groups. The results showed that rBMSCs proliferation increased in an HA content dependent manner, and cells proliferated more in the IGF-1 coated and DOPA-IGF-1 coated groups, in particular in DOPA-IGF-1 coated group, and the differences were more remarkable over time ( P <0.05). HA/PLGA microspheres promoted the proliferation and osteogenic differentiation of rBMSCs, and DOPA-IGF-1 coating enhanced the proliferation and osteogenic differentiation of rBMSCs.
研究领域:
生物材料学
·
细胞生物学
·
再生医学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2016
·
IF 2.76
Ting Yang,Xuejun Cui,Yanbing Kao,Hongyan Wang,Jihong Wen
Hydroxyapatite (HA) nanoparticles were modified by oleic acid (OA) to improve their lipophilicity and the dispersibility in polymer. XRD and FTIR demonstrated that the surface modification did not change the chemical properties of HA. OA modified HA (OA-HA) nanoparticles were introduced into polytrimethylene carbonate/gelatin (PTMC/Gt) composite fiber scaffolds (CFSs) by electrospinning. Scanning electron microscope (SEM) showed that the distribution of OA-HA nanoparticles was more uniform than that of HA nanoparticles in the CFSs. Comparing PTMC/Gt CFSs, PTMC/Gt/HA CFSs and PTMC/Gt/OA-HA CFSs, PTMC/Gt/OA-HA CFSs showed the best mechanical property and more hydrophilic. Cell adhesion and proliferation assay (MTT assay) and confocal laser scanning microscopy (CLSM) demonstrated that the PTMC/Gt/OA-HA CFSs could provide a higher cell adhesion and proliferation environment for mandibular condylar chondrocytes(MCCs). Overall the PTMC/Gt/OA-HA CFSs have nontoxicity and good biocompatibility with MCCs and would be potential to be used in the field of bone and cartilage tissue engineering scaffolds in the future.
研究领域:
生物材料学
·
组织工程学
·
医学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）