Bone defect repair is a hot topic in tissue engineering, and the development of economical and effective bone scaffold materials to achieve the goal of repairing bone defects is an important task for researchers. In this experiment, fresh bovine cancellous bone was used as a xenogeneic
bone material. After two high-temperature calcinations, β-tricalcium phosphate (β-TCP) material was obtained. An in-situ co-precipitation method was employed to prepare hydroxyapatite+graphene oxide (HA+GO) composite material. Then, the β-TCP material
was immersed in the HA+GO composite material solution to prepare β-TCP+HA+GO composite material. The surface modification of β-TCP material with HA and GO improved the elastic modulus of the raw material. In vitro cell showed that the composite material was non-toxic,
biocompatible, and promoted the proliferation of MC3T3-E1 cells on the β-TCP+HA+GO material. Finally, it was found that β-TCP+HA+GO composite material effectively stimulated the actin and mineralization behavior of MC3T3-E1 cells, and promoted the expression of bone-related
factors such as Alkaline phosphatase (ALP), Collagen I (COLI), Osteocalcin (OCN), Bone Morphogenetic Protein-2 (BMP-2), and Bone Morphogenetic Protein-7 (BMP-7) in MC3T3-E1 cells In conclusion, β-TCP+HA+GO composite material can be applied in the biomedical field as a bone substitute
or repair material.
Li Congcong,Zhao Li,Chen Xiaotao,Aierken Aikepaer,Yu Min,Kong Chuifan,Baheti Wufanbieke
Background Pure titanium and titanium alloy implants have been widely utilized in dental implantology due to their excellent biocompatibility and favorable elastic modulus. However, the inherent bioinertness of titanium-based implant surfaces often results in suboptimal osseointegration with surrounding bone tissue. Consequently, surface modification is essential to enhance the osseointegrative capacity of titanium implants. Methods We fabricated hydroxyapatite-graphene oxide (HA-GO) coatings via electrochemical deposition and systematically compared four distinct protein loading methods. Ultimately, we developed a hydroxyapatite-graphene oxide/interleukin-4 (Ti@HA-GO@IL-4) coating on pure titanium substrates to investigate its physicochemical properties and biocompatibility. Results The composite coating was successfully fabricated on pure titanium substrates, inducing significant surface modifications including the incorporation of bioactive functional groups, enhanced hydrophilicity, and altered surface morphology. Comparative characterization revealed that the composite coating exhibited superior hydrophilic properties, improved mechanical compatibility with bone tissue, stable Interleukin-4 (IL-4) adsorption and sustained-release behavior, as well as excellent biosafety confirmed by proliferation and adhesion assays using mouse embryo osteoblast precursor (MC3T3-E1) cells, when compared to unmodified titanium controls. Conclusions Ti@HA-GO@IL-4 represents a composite coating material with exceptional physicochemical properties and biological performance. This material demonstrates efficient IL-4 loading capacity and sustained release characteristics, thereby effectively promoting osteogenesis. These findings provide valuable insights for developing bioactive functional materials to enhance implant osseointegration.
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