NEW JOURNAL OF CHEMISTRY
·
2024
·
IF 2.7
Xiaojing Cui,Yaping Zhang,Hanmeng Zhang,Huaixia Yang,Yanju Liu,Fuchun Si
The sensitive detection of down-regulator of transcription 1 (DR1) plays an important role in the early diagnosis and treatment of Hashimoto's thyroiditis (HT). Herein, we report a novel electrochemical sensor based on the nanocomposite of tungsten oxide–gold nanoparticles (WO3@AuNPs) and hyperbranched polymer–2-bromo-isobutyryl bromide (HBP@BIBB) initiated atom transfer radical polymerization (ATRP) for the ultrasensitive assay of DR1. Monolayer WO3 nanosheets, which have high conductivity, were first compounded with AuNPs to obtain the WO3@AuNP nanocomposite. WO3@AuNPs can not only increase the conductivity of the electrode but also provide more active sites for biological molecules, thereby effectively amplifying the output signal. By introducing macroinitiator HBP@BIBB into the ATRP reaction, more electroactive molecules were introduced into the sensor, further amplifying the output signal and making the sensor ultra-sensitive. The optimized sensor demonstrated a wide linear range of 1 × 10−4 –1 × 102 ng mL−1 with a low detection limit of 2.91 fg mL−1. Moreover, the electrochemical biosensor has high selectivity, good stability, and strong anti-interference ability, and is suitable for the determination of DR1 in complex biological matrices. In conclusion, this electrochemical biosensor shows great potential in biomarker detection.
研究领域:
生物化学
·
分析化学
·
纳米技术
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
·
2024
·Vol. 28
·期 23
·e70271
·
IF 4.3
Zongrui Yang,Yuanyuan Feng,Mingcai Zhang,Yongming Liu,Yizhe Xiong,Xiang Wang,Ying Shi,Bo Chen,Zhengming Wang,Haiya Ge,Hongsheng Zhan,Zhibi Shen,Guoqing Du
HBP-A is the main active component of a traditional Chinese medicine Huaizhen Yanggan Capsule, for the remarkable treatment of knee osteoarthritis (KOA). This study aimed to elucidate the ameliorative effect of HBP-A on meniscus hypertrophy and mineralisation in KOA and the molecular mechanism of its action. An Hartley guinea pig model of KOA that underwent anterior cruciate ligament transection (ACLT) and a model of rat primary meniscus fibrochondrocytes (PMFs) were used to investigate the ameliorative effect of HBP-A on meniscal hypertrophy and calcification and its signal transduction mechanism of action. The results show that Guinea pig's meniscus width, as well as the area of meniscus calcification and meniscus and articular cartilage injury score, were significantly reduced in the HBP-A intervention group compared to the ACLT group. The expression levels of mtrix metalloproteinase 13 (MMP13), runt-related transcription factor 2 (Runx2), Indian hedgehog (Ihh), alkaline phosphatase (ALP), and ankylosis homologue (ANKH) at the protein and gene level significantly decreased in the HBP-A intervention group compared to the ACLT group. In vitro study, apoptosis, hypertrophy, and calcification of rat PMFs after 10% stretch force were significantly improved with HBP-A intervention. Western blot and RT-qPCR showed that hypertrophy, calcification, and p38 MAPK signalling pathway-related markers of PMFs were incredibly depressed in the HBP-A intervention group compared to the 10% stretch force group. In conclusion, HBP-A can slow down meniscus hypertrophy and mineralisation induced by abnormal mechanical loading, and its mechanism of action may be through the p38-MAPK signalling pathway.
研究领域:
药理学
·
骨科
·
分子生物学
·
传统中药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Yan Gao,Junpeng Shi,Chen Wu,Longlong Cao,Lin Liu,Jinyuan Wang,Xiaofang Luo,Xiao Zhang,Yun Zhang
Immunochromatography is a commonly used immediate detection technique, using signal labels to generate detection signals for rapid medical diagnosis. However, its detection sensitivity is affected by background fluorescence caused by the excitation light source. We have developed an immunochromatographic test strip using Zn2GeO4:Mn2+ (ZGM) persistent luminescent nanoparticles (PLNPs) for immediate fluorescence detection and highly sensitive persistent luminescence (PersL) detection without background fluorescence interference. ZGM emits a strong green light when exposed to ultraviolet (UV) excitation, and its green PersL can persist for over 30 min after the excitation light is turned off. We modified the surface of ZGM with heparin-binding protein (HBP) antibodies to create immunochromatographic test strips for the detection of HBP as the target analyte. Under UV excitation, the chromatography test paper can be visually observed at concentrations as low as 25 ng/mL. After the excitation light source is switched off, PersL can achieve a detection limit of 4.7 ng/mL without background interference. This dual-mode immunochromatographic detection, based on ZGM, shows great potential for in vitro diagnostic applications.
研究领域:
生物医学工程
·
诊断学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2023
·
IF 4.1
Libin Huang,Hao Teng,Meiling Wang,Jing Fang,Yu Yuan,Ming Ma,Ziwei Luo,Bo Chen,Bin Guo
Aldehyde-containing metabolites are reactive electrophiles that have attracted extensive attention due to their widespread occurrence in organisms and natural foods. Herein we described a newly-designed Girard's reagent, 1-(4-hydrazinyl-4-oxobutyl)pyridin-1-ium bromide (HBP), as charged tandem mass (MS/MS) tags to facilitate selective capture, sensitive detection and semi-targeted discovery of aldehyde metabolites via hydrazone formation. After HBP labeling, the detection signals of the test aldehydes were increased by 21–2856 times, with the limits of detection were 2.5–7 nM. Upon isotope-coded derivatization with a pair of labeling reagents, HBP- d 0 and its deuterium-labeled counterpart HBP- d 5 , the aldehyde analytes were converted to hydrazone derivatives, which generated characteristic neutral fragments of 79 Da and 84 Da, respectively. The isobaric HBP- d 0 /HBP- d 5 labeling based LC-MS/MS method was validated by relative quantification of human urinary aldehydes (slope=0.999, R 2 > 0.99, RSD s ≤ 8.5%) and discrimination analysis between diabetic and control samples. The unique isotopic doubles (Δ m/z = 5 Da) by dual neutral loss scanning (dNLS) provided a generic reactivity-based screening strategy that allowed non-targeted profiling and identification of endogenous aldehydes even amidst noisy data. The LC-dNLS-MS/MS screening of cinnamon extracts led to finding 61 possible natural aldehydes and guided discovery of 10 previously undetected congeners in this medicinal plant.
研究领域:
分析化学
·
质谱学
·
代谢组学
·
天然产物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2022
·Vol. 40
·期 12
·1447-1456
·
IF 5.56
Jianlin Lv,Yajun Xu,Yue Huang,Haiyang Yu,Zhaohui Tang
Comprehensive Summary Plinabulin, a potential vascular disrupting agent (VDA), has entered the phase III clinical trial. However, the antitumor efficacy of Plinabulin is only moderate, which is probably because of the short action time of acting on tubulin. To maintain a constant VDAs concentration around the tumor vascular endothelial cells, in this study, we developed a novel Plinabulin derivate named HBP and further prepared noncovalent HBP nanoparticles (HBP NPs). We examined and compared the antitumor efficiency of HBP and HBP NPs in 4T1 tumor-bearing mice. HBP NPs significantly increased therapeutic efficiency than HBP, even with a quarter dose. These findings exhibited in this paper further confirmed the opinion that VDAs nanomedicine could markedly enhance tumor therapeutic effect more than small molecular VDAs.
研究领域:
药理学
·
肿瘤学
·
纳米医学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 62
·38982-38989
·
IF 3.361
Zang Chuanfeng,Han Xiangye,Dong Erying,Shen Feiyu,Yan Tingting,Wang Runyue,Zhang GuangyuYu
A new type of nanofiber membrane was prepared by mixed electrospinning of cellulose diacetate and amino-terminated hyperbranched polymer (HBP-NH2), and glutaraldehyde (GA) crosslinking was used to improve its water resistance and shape retention. pH, adsorption time, and initial solution concentration on Cu(II) and Cr(VI) ions were studied. Results showed that the prepared GA-CDA/HBP-NH2 nanofiber had good spinning uniformity, hydrophilicity, and adsorption effect on Cu(II) and Cr(VI) ions compared with the pure CDA nanofiber. The maximum adsorption capacity of GA-CDA/HBP-NH2 for Cu(II) and Cr(VI) was 50.2 and 176.6 mg g−1, respectively.
研究领域:
材料科学
·
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTRONIC MATERIALS
·
2021
·Vol. 50
·期 6
·3222-3227
·
IF 1.938
Han Wensong,Guo Qin,Su Tianhui
Cu@HBP-SH@Ag particles have been prepared by a facile synthesis strategy. Initially, thiol-terminated hyperbranched polymer (HBP-SH) was synthesized by stepwise polymerization. Subsequently, Cu particles covered with HBP-SH were prepared by reduction technology using HBP-SH as dispersant and template. Finally, Cu@HBP-SH@Ag particles were obtained by reduction reaction. Fourier-transform infrared (FT-IR) and ultraviolet–visible (UV–Vis) spectroscopy confirmed presence of HBP-SH on the surface of the Cu particles. The crystal structure was examined by x-ray diffraction (XRD) analysis. Scanning electron microscopy (SEM) and energy-dispersive spectrometry (EDS) analysis confirmed formation of Cu@HBP-SH@Ag particles. Thermogravimetric analysis (TGA) showed that the Cu@HBP-SH@Ag particles had better antioxidation properties than the Cu particles.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2020
·
IF 3.99
Yan Li,Shuai Wang,Fang Xiang Song,Li Zhang,Wei Yang,Hong Xia Wang,Qian Lin Chen
Mesoporous silica nanoparticles with folic acid (MSN−COOH-Tet-HBP-FA) are able to mediate targeting and have a pH stimulation response character, and therefore have been successfully synthesized as vectors for antitumor drug delivery. The model drug is tetrandrine (Tet). The chemical structure and properties of these NPs were characterized through systematic characterization analyses. Their drug loading capacity was significantly improved through carboxy modification (26.86 % in max), and they exhibited pH-dependent drug release profiles("zero pre-release" within 20 h in a normal physiological environment). In vitro cytotoxicity and cell uptake of the as-synthesized NPs in Hela and A549 cells were also evaluated, and exhibited high cytotoxicity and cell-targeted uptake capacity. It was concluded that MSN−COOH-Tet-HBP-FA could be used as a promising drug delivery system for cancer therapy.
研究领域:
药剂学
·
肿瘤学
·
纳米医学
✓ 已验证
产品关联
DOI
高影响因子
PROCESS BIOCHEMISTRY
·
2023
·
IF 4.4
Dongxu Feng,Jun Wang,Pingfa Gao,Dianbao Gu,Wenbing Li,Lijuan Shi,Dawei Chen,Gangfeng Hu,Wei Wu
As vectors for anticancer drug delivery, mesoporous silica nanocomposites (MSN) incorporated bicalutamide (BLT) featuring folic acid (FA) (termed as COOH-MSNs-HBP@BLT/FA) have been effectively achieved due to their ability to pH stimulation response and targeting. Systematic characterization investigations were conducted to determine the chemical composition and physical characteristics of these NPs. Through carboxy alteration, their drug loading capacity was dramatically increased (268.60 %), and they displayed pH-responsive drug release patterns. Additionally, the cytotoxicity of the as-synthesized NPs was tested in MDA-MB-231 and MCF-7 cells in vitro. After 24-h of treatment with BLT, COOH-MSNs, COOH-MSNs@BLT, COOH-MSNs-HBP@BLT/FA, MCF-7, and MDA-MB-231 cells exhibited distinct morphological alterations, and reddish orange apoptotic bodies were detected using acridine orange and ethidium bromide (AO and EB) double staining. Nuclear alterations such as chromatin condensation/fragmentation were detected by Hoechst 33342 staining techniques. The Annexin V-FITC/PI was used to examine the apoptosis mechanism of the cells. These results prove that apoptosis induction is responsible for the antiproliferative effects of BLT, COOH-MSNs, COOH-MSNs@BLT, and COOH-MSNs-HBP@BLT/FA in MCF-7 and MDA-MB-231cells. We conclude that COOH-MSNs-HBP@BLT/FA has the potential as a drug delivery method for breast cancer treatment.
研究领域:
药学
·
肿瘤学
·
纳米医学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Jiajun Yang,Siyu Hou,Yuhui Zhao,Zhaoyang Sun,Lilin Zhang,Yan Deng,Xiaoli Shang,Hanjie Yu,Zheng Li,Hongmei Li
The antidiabetic activity of the novel Buckwheat protein-derived peptide (Ala-Phe-Tyr-Arg-Trp, AFYRW) and its associated protein glycosylation have been verified. Our preliminary study demonstrates the potential of AFYRW as a therapeutic agent for diabetes, but the mechanism needs further investigation. Given the vital role of O-linked N -acetylglucosamine transferase (OGT) in diabetes mellitus and insulin resistance (IR), we focused on the underlying molecular mechanisms of them in ameliorating IR. We found AFYRW protects against hyperglycemia in diabetic mice and improves glucose metabolism in an IR cell model. Mechanistically, we demonstrated that AFYRW decreases glutamine-fructose-6-phosphate amidotransferase (GFAT) expression via X-box binding protein 1 (XBP1) in the hexosamine biosynthesis pathway (HBP), consequently decreasing OGT and stimulating the SIRT1/PGC1α pathway. Of note, the overlapping roles of increased SIRT1 and decreased OGT caused by AFYRW ameliorated IR. The data presented here show that AFYRW contributes to metabolism by directly controlling glucose homeostasis. Taken together, our study unveils that AFYRW protects against both insulin resistance and diabetes mellitus-induced hyperglycemia through OGT to regulate the SIRT1/PGC1α pathway, which provides a mechanistic basis for novel AFYRW to be a potential therapeutic agent.
研究领域:
内分泌学
·
分子生物学
·
糖尿病研究
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）