Shanshan Wei,Haolin Xiao,Miao Gu,Zhencheng Chen,Liangli Cao
In this study, a sensitive and quantitative label-free electrochemical immunosensor based on bimetallic [email protected] nanoparticles , Prussian blue (PB), and reduced graphene oxide-tetraethylene pentamine nanocomposite (rGO-TEPA) was constructed to achieve sensitive detection of HBsAg. A one-pot green method was used to synthesize interlocked rGO-TEPA/PB hybrid composites as high-performance materials with good conductivity . PB was the electron transfer mediator used to provide electrochemical signals. By utilizing rGO-TEPA as supporting material , the stability and intensity of PB were greatly improved. The incorporation of highly conductive [email protected] nanoparticles could further amplify the electrochemical signal, and the high adsorption capacity could provide an efficient substrate for hepatitis B surface antibody (HBsAb) immobilization. Under optimal conditions, the immunoassay had a wide linear range from 0.25 pg mL −1 to 400 ng mL −1 and a low detection limit of 0.08 pg mL −1 (S/N = 3). This method was also applied to the analysis of human serum samples, and the obtained results were on par with those of electrochemiluminescence immunoassay (ECLIA), suggesting significant potential for this new method in clinical research.
In this work, a sandwich-type electrochemical immunosensor was fabricated to the effective detection of hepatitis B surface antigen (HBsAg). The designed electrochemical immunosensor was based on Au core and Pd shell nanodendrites loaded on amino functionalized molybdenum dioxide nanosheets (Au@Pd NDS/NH 2 –MoO 2 NSs) as the secondary antibody (Ab 2 ) label and silver nanoparticles were loaded by electrodeposited (D-Ag NPs) on the surface of electrode as the platform. Because of the synergistic effect and abundant catalytic activity sites provided by surface dendrite structure, Au@Pd NDs were more effective than single gold and palladium nanoparticles in catalytic reduction of hydrogen peroxide (H 2 O 2 ). MoO 2 had the good catalytic capacity for reduction of H 2 O 2 and favourable electrical conductivity . Hence, the obtained Au@Pd NDS/NH 2 –MoO 2 NSs were more effective than Au@Pd NDs and NH 2 –MoO 2 NSs in catalytic reduction of hydrogen peroxide attribute to a synergistic effect. Also, Ag NPs with admirable electrical conductivity and biocompatibility were used as sensing platforms and primary antibodies (Ab 1 ) carriers, which can accelerate the electron transfer and improve the sensitivity of the immunosensor. Here, the proposed electrochemical immunosensor offered a wide linear interval from 10 fg mL −1 to 100 ng mL −1 and the lower limit of detection of 3.3 fg mL −1 (S/N = 3) for detection of HBsAg under optimal experimental conditions. Furthermore, the accuracy of the actual serum sample analysis was satisfactory, which showed that the electrochemical immunosensor possessed a good application prospect in clinical detection.
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