查找使用阿拉丁产品的科研文献

Ultrasensitive ELISA for the detection of hCG based on assembled gold nanoparticles induced by functional polyamidoamine dendrimers

Weiguo Wang,Junmin Li,Changzhi Dong,Yafang Li,Qiuye Kou,Jinwu Yan,Lei Zhang

Amplifying the signal of ELISA is important in the early disease diagnosis. Herein we report an ultrasensitive ELISA applying for the detection of hCG based on the assemble of AuNPs induced by functional PAMAM . The AuNP-PAMAM probe shows a competitive advantage sensitivity of 0.03 IU L −1 compared to traditional ELISA and mAb 1 -AuNP-HRP probe. The line range is ranged from 0.1 to 6.4 IU L −1 . Moreover, the precision and reproducibility and specificity of AuNP-PAMAM probe are also eligible for the detection of hCG. The assembled AuNPs was firstly used in the signal enhancement in immunoassay .

HCG supplement did not accelerate tunica albuginea remodeling to facilitate penile growth

Li Tao,Tian Yuan,Zhong Quliang,Chen Peng,Zhang Junhao,Du Guangshi,Li Lei,Jiang Yiting,Jiang Kehua

Penile size is closely concerned and short penis contributes serious sexual dysfunction and tremendous psychological problems to couples. Androgen is essential for penile development and testosterone replacement is recommended to patients with micropenis. We previously proved that inhibiting activity of lysyl oxidase (Anti-lysyl oxidase, Anti-LOX) combined with vacuum erectile device (VED) lengthened penis by remodeling tunica albuginea. We thus explored whether HCG supplement could accelerate tunica albuginea remodeling (induced by Anti-LOX + VED) to promote penile growth. Forty-two SD male rats (4 weeks old) were purchased and divided into 7 groups: control, Anti-LOX, HCG, VED (with a negative aspirated pressure of − 300 mmHg), Anti-LOX + VED, HCG + VED, and Anti-LOX + HCG + VED. After an intervention for 4 weeks, all rats’ penile length, exposed penile length, and erectile function were measured. Serum samples were collected to detect hormone levels and penile corpus cavernosum were harvested for histo-pathological analysis. All intervention groups showed significantly longer penis than controlled rats. Anti-LOX sharply increased penile length and exposed length by 15% and 9% respectively, this lengthening effect was more obvious in Anti-LOX + VED group (26% and 19%, respectively). Although HCG promoted penile length by 8%, this effect was slight for exposed length (3%). Moreover, Anti-LOX + HCG + VED dramatically increased penile length and exposed length by 22% and 18%, respectively, which was similar with that in Anti-LOX + VED (26% and 19%, respectively). HCG dramatically stimulated testosterone and dihydrotestosterone secretions than control group, whether with or without Anti-LOX and VED; while it induced more AR expression than other groups. Finally, all procedures did not improve or deteriorate normal erectile function. Although we verified that Anti-LOX + VED lengthened penis by inducing tunica albuginea remodeling, however, HCG supplement did not synergize with Anti-LOX + VED to accelerate albuginea remodeling to facilitate penile growth.

A Sensitive Immunochromatographic Test Strip Based on Hydrophobic Quantum Dots Incorporated into Mg/Fe Nanoflowers for HCG Detection

Hao Liu,Qing Zhang,Ning Bao,Shou-Nian Ding

As the most widely used disposable paper-based diagnostic tool in the world, immunochromatographic test strips (ICTS) have occupied more and more positions in the field of rapid diagnosis due to their ease of operation and affordability. Therefore, the development of an easily prepared, sensitive, and accurate signal reporter is of great significance for the detection of some low-abundance biomarkers in clinical diagnosis. Herein, Mg/Fe layered double hydroxide nanoflowers (MF NFs) were selected as adsorption templates and sulfhydryl-functionalized, followed by one-step loading of hydrophobic CdSe/ZnS quantum dots in the organic phase via a metal-thiol covalent bond. After coating the reporter with branched polyethyleneimine (PEI), a novel ICTS fluorescent reporter was prepared. The modification of PEI not only improved the hydrophilicity of MF@CdSe/ZnS NFs but also introduced amino functional groups on the surface of the reporter for subsequent conjugation with antibodies. X-ray photoelectron spectroscopy, UV-vis absorption, X-ray diffraction, fluorescence spectroscopy, and infrared spectroscopy were used to characterize the composition of MF@CdSe/ZnS NFs. Under the optimal experimental conditions, the detection range of MF@CdSe/ZnS@PEI-ICTS for the model analyte HCG was 0.1–500 mIU/mL, and the limit of detection (LOD) reached was 0.1 mIU/mL. The potential for practical application was validated by the detection of HCG in spiked healthy human serum, showing overall recoveries between 90.48 and 116.1% with coefficients of variation that ranged from 3.66 to 12.91%.

Detection of botulinum neurotoxin A (BoNT/A) enzymatic activity by pregnancy test strips based on hCG-modified magnetic nanoparticles

Zhou Xinxin,Qi Min,Yang Minghui

The detection of botulinum neurotoxin A (BoNT/A) endopeptidase activity by pregnancy test paper based on human chorionic gonadotropin (hCG)-functionalized peptide-modified magnetic nanoparticles (MNs) is described for the first time. HCG-functionalized SNAP-25 peptide substrate with hydrolysis recognition sites was optimally designed. HCG can be recognized by pregnancy test strips. BoNT/A light chain (BoNT-LcA) is the central part of the endopeptidase function in holotoxin, which can specifically hydrolyze SNAP-25 peptide to release the hCG-peptide probe, and the hCG-peptide probe released can be quantitatively detected by pregnancy test strips, achieving indirect determination of BoNT/A. By quantifying the T-line color intensity of test strips, the visual detection limit for BoNT-LcA is 12.5 pg/mL, and the linear range of detection for BoNT-LcA and BoNT/A holotoxin was 100 pg/mL to 1 ng/mL and 25 to 250 ng/mL. The ability of the method to quantify BoNT/A was validated in human serum samples. This method shows the potential for sensitive detecting BoNT/A and has prospects for the diagnosis and prognosis of clinical botulism.

A fluorescent immunochromatographic strip based on CsPbBr3/CsPb2Br5@SiO2 facile encapsulation in pure water for the detection of human chorionic gonadotropin (HCG) antigen

Jingcheng Zheng,Jie Chen,Ruifen Sun,Weiwei Zhang,Yun Huang,Jiwei Shao,Yuwu Chi

Fluorescent immunochromatographic test (FICT) strips have received extensive attention due to their virtue of simple operation, low cost, and a point-of-care (POC) assay. In recent years, people are constantly seeking new labels to improve the sensitivity of immunosensors. Perovskite nanocrystals (PNCs) possess strong fluorescence and a narrow emission peak, making them an excellent choice as a new fluorescent signal label. Herein, we have developed a simple, fast, and environmentally friendly method to synthesize CsPbBr 3 /CsPb 2 Br 5 @SiO 2 with the good water stability and high biocompatibility. These CsPbBr 3 /CsPb 2 Br 5 @SiO 2 have been applied to construct FICT strips. To achieve the quantitative determination of human chorionic gonadotropin (HCG), a lab-made FICT strips detection system was established. The FICT strips had a good linear relationship to HCG in the range of 5–500 ng/mL, and the detection limit was 2.09 ng/mL. In addition, the recovery results of HCG detection in human urine samples also proved the feasibility of this FICT strips. CsPbBr 3 /CsPb 2 Br 5 @SiO 2 can also be modified with different biomarkers for functionalization, which exhibits a promising application in the development of sensing platform of FICT strips.

Label free hCG concentration biosensor based on suspension core fiber microfluidic technology

Xuegang Li,Xindi Zhang,Xue Zhou,Yong Zhao,Linh Viet Nguyen,Yanan Zhang,Stephen C. Warren-Smith

Low-toxicity, water-dispersible perovskite@COF ECL biosensor: Peptide-regulated AgNP quenching for sensitive hCG detection

Yuanxun Gong,Shunjin Wen,Pengyuan He,Jiayi Zhang,Qianli Tang,Kai Zhang,Xianjiu Liao

s Human chorionic gonadotropin (hCG) is a key clinical marker for early pregnancy and trophoblastic disease. We present a turn-on electrochemiluminescence (ECL) biosensor emphasizing materials safety and aqueous compatibility by integrating a CsPbBr₃@COF-LZU1@Au hybrid with a peptide recognition layer. Confining perovskite nanocrystals inside COF-LZU1 yields a porous, mechanically robust shell that suppresses lead ion release, improves water compatibility/dispersion, and mitigates photochemical and hydrolytic degradation, enabling reliable ECL in PBS. A conformal Au nanodomain rim enhances interfacial charge transport and provides mild, covalent (Au S) anchoring sites for the hCG-binding peptide (PPLRINRHILTR-GGGGC), avoiding bulky antibodies and harsh coupling reagents. Signal regulation is achieved with citrate-stabilized Ag nanoparticles that assemble near the peptide in the intact state to quench ECL; specific hCG binding sterically prevents AgNP tethering, restoring emission. Operating under benign aqueous conditions (no organic solvents during measurement, small reagent volumes, room-temperature assembly), the sensor delivers a wide working range (0.05–5000 mIU/mL), a low detection limit (0.047 mIU/mL), high selectivity over common interferents (FSH, LH, TSH, IgG, BSA), excellent reproducibility (RSD ≈ 2.2 %), and strong intraday/long-term/operational stability. Serum tests give ~96–103 % recoveries with low RSDs, indicating minimal matrix effects. By encapsulating lead-based emitters to reduce toxicity risk, enhancing aqueous dispersibility, and using compact peptide ligands for precise molecular recognition, this modular perovskite@COF/Au/peptide design offers a practical and safer route to high-performance ECL diagnostics and can be readily adapted to additional biomarkers by swapping the peptide sequence.

Effects of dodder total flavone on polycystic ovary syndrome rat models induced by DHEA combined HCG

Mingsan Miao,Mengfan Peng,Zhengwang Zhu,Xiaoli Yan,Zhenzhen Wei,Mengyan Li

Aims Explore the effects of dodder total flavone on polycystic ovary syndrome (PCOS) rat models induced by dehydroepiandrosterone (DHEA) combined human chorionic gonadotropin (HCG). Methods Except the blank group, the rest of the rats were injected with DHEA 6 mg/100 g on the back of the neck and 1.5 IU HCG each day, for 21 consecutive days. On the 16th day of modeling, vaginal smear was performed to select the model rats, which were randomly divided into model group, dacin-35 group, large, middle and small dose dodder total flavonoids groups, and given the medicine for three weeks. At the end of the last administration, take samples, so as to calculate the ovaries and uterus indexes, measure serum LH/FSH ratio, P, PRL and INS levels, fixed the uterus and pancreas in 10% formalin solution and stained with HE to observe the morphological changes of the organs. And measure the expression of TNF-α and IGF-l proteins in ovaries by immunohistochemistry. Results Compared with the blank group, ovarian and uterine indexes, serum LH/FSH ratio, serum PRL and INS levels, ovary TNF-α and IGF-l protein expression were significantly increased, and significant pathological changes were observed in the uterine and pancreatic tissues in model group ( P  < 0.01). While the serum P level decreased significantly ( P  < 0.01), Compared with the model group, the ovarian and uterine indexes, serum LH/FSH ratio, serum P, PRL and INS levels, ovary TNF-α protein expression were significantly decreased in large, middle and small dose dodder total flavonoids groups ( P  < 0.01); The expression of IGF-1 protein was decreased and uterus pathological changes were improved in different extents ( P  < 0.01 or P  < 0.05), pancreas pathological changes were improved significantly ( P  < 0.01). Conclusion PCOS rat models was successfully replicated. Dodder total flavone can protect PCOS rats induced by DHEA combined HCG by different action pathways.

A simple synthesis of VSe2/B4C@HCG composite as high-performance anodes for sodium-ion batteries

Hongwei Zhang,Deping Xiong,Yandong Xie,Hongli Zhang,Zuyong Feng,Kunhua Wen,Zhaoying Li,Miao He

Sodium-ion batteries (SIBs) arouse researchers’ interest gradually because of the scarcity of lithium sources. VSe 2 is one of the transition metal dichalcogenides (TMDs) equipped with a huge interlayer distance to accommodate more Na + ions, but the problems such as agglomeration, volume deformation, dissolution loss and so on hinder its further development. Therefore, a VSe 2 /B 4 C@HCG composite was fabricated by the simple high-energy ball milling method. The B 4 C and VSe 2 nanoparticles are mixed and restrict each other, then anchor on the graphene, which alleviates agglomeration and volume deformation of VSe 2 during cycling processes effectively, and improves the overall environmental stability and conductivity. As a result, the VSe 2 /B 4 C@HCG composite delivers a reversible capacity of 407.5 mAh g −1 with the coulombic efficiency of 98.5% after 450 cycles, superior rate performance of 524.2, 476.3, 413.7, 353.7, and 295.3 mAh g −1 at the different current density of 100, 200, 500, 1000, and 2000 mA g −1 , and ultrastable long-term cycling property of 251.6 mAh g −1 at the current density of 1 A g −1 after 1000 cycles, indicating the VSe 2 /B 4 C@HCG composite shows utilization potentiality to be used as an excellent anode material for SIBs.

Ultrasensitive colorimetric immunoassay for hCG detection based on dual catalysis of Au@Pt core–shell nanoparticle functionalized by horseradish peroxidase

Weiguo Wang,Yake Zou,Jinwu Yan,Jing Liu,Huixiong Chen,Shan Li,Lei Zhang

In this paper, an ultrasensitive colorimetric biosensor for human chorionic gonadotrophin (hCG) detection was designed from bottom-up method based on the dual catalysis of the horseradish peroxidase (HRP) and Au@Pt nanoparticles (NPs) relative to H 2 O 2 -TEM system. HRP and monoclonal mouse anti-hCG antibody (β-submit, mAb 1 ) were co-immobilized onto the Au@Pt NP surface to improve catalytic efficiency and specificity, which formed a dual functionalized Au@Pt-HRP probe with the mean size of 42.8   nm (D 50 ). The colorimetric immunoassay was developed for the hCG detection, and the Au@Pt-HRP probe featured a higher sensitivity in the concentration range of 0.4–12.8   IU   L −   1 with a low limit of detection (LOD) of 0.1   IU   L −   1 compared with the LODs of 0.8   IU   L −   1 for BA-ELISA and of 2.0   IU   L −   1 for Au@Pt, which indicated that the Au@Pt-HRP probe possessed higher catalytic efficiency with 2.8-fold increase over Au@Pt and 33.8-fold increase over HRP. Also, the Au@Pt-HRP probe exhibited good precision and reproducibility, high specificity and acceptable accuracy with CV being less than 15%. The dual functionalized Au@Pt-HRP probe as a type of signal amplified method was firstly applied in the colorimetric immunoassay for the hCG detection.

Ginkgo biloba extract 761 reduces vascular permeability of the ovary and improves the symptom of ovarian hyperstimulation syndrome in a rat model

Jie Zhang,Jie Huang,Xinhuan He,Ning Li,Yu Miao,Beiqing Li,Xiaoguang Shao,Ningning Wang

Aims Ginkgo biloba extract (EGb) has been widely applied in the treatment of cerebrovascular and neurological diseases. However, the effect of EGb761 on ovarian hyperstimulation syndrome (OHSS), a vascular disorder and life-threatening complication of in vitro fertilization and intracytoplasmic sperm injection therapy (IVF/ICSI), has not been evaluated.Materials and methods Forty female Wistar rats aged 22-days old (D22) were divided into eight groups: Control rats received intraperitoneal injection of saline for five consecutive days (D22–D26); OHSS model group received 10 IU equine chorionic gonadotropin (eCG) for four consecutive days (D22–D25) and 30 IU of human chorionic gonadotropin (hCG) on the 5th day (D26); Prophylactic treatment group received three doses of EGb761 (50, 100, and 200 mg/kg/day) 1 h before injection of eCG (hCG) for seven consecutive days; Therapeutic treatment group received three doses of EGb761 (50, 100, and 200 mg/kg/day) 48 h after injection of eCG (hCG) for seven consecutive days.Results All three doses of EGb761 therapeutic medication significantly reduced ovarian mass index of OHSS model rats (p ≤ .01). Furthermore, therapeutic treatment group exhibited improved vascular permeability, decreased estradiol and progesterone levels, lower corpus luteum, and higher follicle numbers compared with the OHSS model. Elevated protein expression of vascular endothelial growth factor (VEGF) and VEGF receptor (VEGFR) in both ovary and kidney of the OHSS model was restrained by EGb761 therapeutic treatment.Conclusions EGb761 therapeutic medication decreases vascular permeability in OHSS rat model by inhibiting VEGF and VEGFR expression, which may contribute to the treatment of OHSS.

One-step detection of glutathione based on MnO2 nanosheet and pregnancy test strip

Huai Yang,Xiao-Shuai Zhang,Kai Cheng,Bo Liu,Wei Chen,Jin-Xuan Fan,Yuan-Di Zhao

Low cost and rapid detection of glutathione (GSH) has important clinical reference value for the diagnosis and treatment of related diseases. In this study, a one-step method for rapid detection of GSH is developed based on the characteristics of low cost, simple operation and rapid color development of pregnancy test strip. In this method, magnetic Fe 3 O 4 -doped MnO 2 nanosheet is first synthesized, and colloidal gold probe modified with hCG and ssDNA is absorbed on the surface of the nanosheet. Based on the oxidase-like properties of MnO 2 nanosheet, in the presence of GSH, a redox reaction between GSH with MnO 2 occurs to release hCG signal probe into the solution. The supernatant solution is loaded on pregnancy test strip after magnetic separation, and the test line develops color. Then the smartphone App developed in our lab is used to quantitatively detect GSH. The results show that under the optimal conditions, the GSH in the range of 40~800   μM has a good linear relationship with the color intensity of the test line on pregnancy test strip and the detection limit for GSH is 40   μM. So, this method can realize the detection of GSH with low cost, simple, rapid and sensitive, and it can meet the requirements of GSH detection in actual samples.