INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
·
2018
·
IF 2.317
Dan-Ting Yin,Yu Fu,Xin-Huai Zhao
Inulin was fermented by adult faecal microbiota and 10 exogenous strains for 24 or 48 h. The contents of acetate, propionate, butyrate and lactate were quantified in the fermented products, and the growth-inhibitory and apoptosis-inducing effects on a human colon cell line (HCT-116 cells) were assessed. Most of these strains increased contents of acetate, propionate and butyrate, and promoted lactate conversion. Correlation analysis suggested that butyrate and lactate in the fermentation products were positively and negatively correlated with the measured inhibition ratios (p < .05). The results were mostly consistent with the verification trial results using standard acid solutions. The fermentation products could cause apoptosis via inducing DNA fragmentation and increasing total apoptotic populations in the treated cells. Moreover, the fermentation products with higher growth-inhibitory activities demonstrated the increased apoptosis-inducing properties. In conclusion, these strains could cooperate with adult faecal microbiota to confer inulin fermentation products with higher anti-colon cancer activity.
研究领域:
微生物学
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癌症研究
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胃肠病学
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产品关联
DOI
PubMed
高影响因子
Current Research in Food Science
·
2022
·
IF 6.269
Ya-Hui Yu,Li Wang,Qiang Zhang,Xiao-Nan Zhang,Xin-Huai Zhao
The soluble and non-digestible longan ( Dimocarpus longan Lour . ) polysaccharides (LP) with Se content less than 0.01 g/kg were extracted and selenylated chemically with the HNO 3 –Na 2 SeO 3 system, to prepare two selenylated products namely SeLP1 and SeLP2 with enhanced Se contents of 1.46 and 4.79 g/kg, respectively. LP, SeLP1, and SeLP2 were then measured and compared for their saccharide features and bioactivity in human colon carcinoma HCT-116 cells. Compared with LP, both SeLP1 and SeLP2 contained more neutral saccharides, but showed reduced uronic acid content and undetectable sulfate. Moreover, SeLP1 and especially SeLP2 in the cells showed higher activities than LP, reflected by their enhanced capacity to inhibit cell growth, alter cell morphology, and suppress cell colony formation. Compared with LP, SeLP1 and especially SeLP2 were also more capable of promoting intracellular reactive oxygen species and Ca 2+ levels, causing mitochondrial membrane potential loss, or inducing cell apoptosis via up- and down-regulating the eight apoptosis-related genes and proteins. Overall, the performed chemical selenylation of LP resulted in obvious changes in these saccharide features and simultaneously enhanced the anti-cancer activity of the selenylated products against the cells clearly, while a higher selenylation extent of the selenylated products consistently caused higher activity towards the cells. The results of this study thus highlighted that this chemical selenylation is applicable when aiming to enhance the bioactivities of natural polysaccharides.
研究领域:
药理学
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癌症研究
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生物化学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 23
·3748
·
IF 5.561
Ling-Yu Li,Qing-Yun Guan,Ya-Ru Lin,Jun-Ren Zhao,Li Wang,Qiang Zhang,Hong-Fang Liu,Xin-Huai Zhao
The anti-cancer effects of selenylated plant polysaccharides are a focus of research. As a natural plant with extensive biological effects, there have been few studies related to edible purslane (Portulaca oleraceaL.). Thus, in this study, solubleP. oleraceapolysaccharides (PPS) were extracted from the driedP. oleraceaand then selenylated chemically using the HNO3-Na2SeO3method to obtain two selenylated products, namely, SePPS1 and SePPS2. Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides—arabinose, fucose, and ribose—and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition. Moreover, SePPS1 and SePPS2 shared similar features with respect to monosaccharide composition and possessed higher bioactivity than PPS in human colon cancer HCT-116 cells. Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway. SePPS2 consistently showed the highest capacity to exert these observed effects on the targeted cells, suggesting that the performed chemical selenylation of PPS (in particular when higher degrees of selenylation are reached) resulted in an increase in activity in the cells. It can thus be concluded that the performed selenylation of PPS was able to incorporate inorganic Se into the final PPS products, changing their monosaccharide composition and endowing them with enhanced nutraceutical and anti-cancer effects in the colon.Keywords:purslane;polysaccharide;selenylation;monosaccharide composition;HCT-116 cells;nutraceutical effect
研究领域:
药理学
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癌症研究
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生物化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2023
·Vol. 21
·期 1
·1-14
·
IF 10.2
Liu Guoyong,Wang Shuxian,Wang Shumin,Wu Rongrong,Li Hui,Zha Menglei,Song Jibin,Yin Yuxin,Li Kai,Mu Jing,Shi Yu
Background Photothermal therapy (PTT) in the second near-infrared (NIR-II) window has attracted extensive attention due to the benefits in high maximum permissible exposure and penetration depth. Current photothermal agents generally show a broadband absorption accompanied by a gradual attenuation of absorption in the NIR-II window, leading to poor effect of PTT. It remains a great challenge to gain photothermal agents with strong and characteristic absorption in NIR-II regions. To overcome this problem, based on carbon dots (CDs)-mediated growth strategy, we proposed a simple and feasible approach to prepare plasmonic gold nanodendrites (AuNDs) with NIR-II absorption to enhance the therapeutic effect of PTT. Results By rationally regulating the size and branch length of AuNDs, the AuNDs exhibited a broadband absorption from 300 to 1350 nm, with two characteristic absorption peaks located at 1077 and 1265 nm. The AuNDs demonstrated desired optical photothermal conversion efficiency (38.0%), which was further applied in NIR-II photoacoustic imaging (PAI) and PTT in human colon cancer cells (HCT 116)-tumor-bearing mice model. The tumor cells could be effectively eliminated in vivo under 1064 nm laser irradiation by the guidance of PAI. Conclusions We reported a simple but powerful synthetic method to obtain the unique AuNDs with strong and characteristic absorption peaks in the NIR-II window. This study provides a promising solution to tuning the growth of nanoparticles for bioimaging and phototherapy in the NIR-II window. Graphical Abstract
研究领域:
纳米技术
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生物医学工程
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材料科学
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癌症治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 7
·6854
·
IF 5.6
Mengwen Guo,Junhong Ling,Xinyi Xu,Xiaokun Ouyang
In clinical cancer research, photothermal therapy is one of the most effective ways to increase sensitivity to chemotherapy. Here, we present a simple and effective method for developing a nanotherapeutic agent for chemotherapy combined with photothermal therapy. The nanotherapeutic agent mesoporous polydopamine-Fe(III)-doxorubicin-hyaluronic acid (MPDA-Fe(III)-DOX-HA) was composed of mesoporous polydopamine modified by ferric ions and loaded with the anticancer drug doxorubicin (DOX), as well as an outer layer coating of hyaluronic acid. The pore size of the mesoporous polydopamine was larger than that of the common polydopamine nanoparticles, and the particle size of MPDA-Fe(III)-DOX-HA nanoparticles was 179 ± 19 nm. With the presence of ferric ions, the heat generation effect of the MPDA-Fe(III)-DOX-HA nanoparticles in the near-infrared light at 808 nm was enhanced. In addition, the experimental findings revealed that the active targeting of hyaluronic acid to tumor cells mitigated the toxicity of DOX on normal cells. Furthermore, under 808 nm illumination, the MPDA-Fe(III)-DOX-HA nanoparticles demonstrated potent cytotoxicity to HCT-116 cells, indicating a good anti-tumor effect in vitro. Therefore, the system developed in this work merits further investigation as a potential nanotherapeutic platform for photothermal treatment of cancer.Keywords:mesoporous polydopamine;ferric ions;doxorubicin (DOX);hyaluronic acid target modification
研究领域:
生物医学工程
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2025
·
IF 2.5
Shao-Jun Zheng,Xiao-Han Diao,Xiao-Yan Gao,Nai-Dong Chen
Dendrobium officinale polysaccharides (DOP) exhibit anticancer potential against colorectal cancer (CRC), yet their mechanisms remain unclear. This study investigated DOP's effects on HCT-116 cells. DOP dose- and time-dependently inhibited proliferation and induced apoptosis, evidenced by elevated cleaved caspase-3/-9, poly ADP-ribose polymerase (PARP), Bax, and reduced Bcl-2. Concurrently, DOP activated autophagy (increased LC3-II/I, Beclin-1; decreased p62) via phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/Akt/mTOR) suppression. Combining DOP with the autophagy inhibitor chloroquine enhanced apoptosis (higher cleaved PARP, Bax; lower Bcl-2) and reactivated PI3K/Akt/mTOR signaling. These findings demonstrate DOP inhibits CRC by dual induction of apoptosis and autophagy blockade through PI3K/Akt/mTOR inhibition, with autophagy suppression synergistically augmenting apoptosis. The study highlights DOP's translational potential as a functional food or adjuvant therapy, proposing a novel combinatorial strategy for CRC treatment. Graphical Dendrobium officinale polysaccharides (DOP) inhibit HCT-116 colorectal cancer cell proliferation and induce apoptosis by upregulating cleaved caspase-3/-9, PARP, and Bax while downregulating Bcl-2. DOP activates autophagy via PI3K/Akt/mTOR inhibition. Combining DOP with chloroquine enhances apoptosis, revealing autophagy blockade as a synergistic anticancer mechanism.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Ya-Hui Yu,Kouadio Jean Eric-Parfait Kouame,Xiaozhen Liu,Xiangying Yu,Ming-Yu Jin,Long-Qing Li,Fengyuan Liu,Yuting Li,Jing-Kun Yan,Bing Li
The combination of selenium nanoparticles (SeNPs) with natural polysaccharides represents an effective strategy to address the limitations of SeNPs and providing a stable, highly bioactive form of Se. In this study, we explored the potential of longan polysaccharides (LP) in the preparation and stabilization of SeNPs. The morphology, stability, stabilization mechanism, and cytotoxicity of LP-SeNPs were investigated. The results showed that the LP-SeNPs were uniform spherical nanoparticles with a mean particle size of 100.4 ± 1.1 nm, and demonstrated excellent stability over a 14-day storage period, which was attributed to the interaction between the SeNPs and the hydroxyl groups present on the surface of LP. Meanwhile, LP-SeNPs have no toxic effect on rat intestinal epithelial (IEC-6) cells, while they are cytotoxic to human colon cancer (HCT-116 and HT-29) cells, including growth inhibition, morphological alteration, and the promotion of reactive oxygen species and mitochondrial membrane potential loss in a dose-dependent manner. LP-SeNPs induced apoptosis in HCT-116 and HT-29 cells by regulation of the apoptosis-related proteins including Bax, Bcl-2, caspase-3, and cytochrome c . Therefore, our results provide a theoretical basis for the development and application of LP and indicate the potential promise of LP-SeNPs as dietary Se supplements and health-promoting food ingredients.
研究领域:
食品科学
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生物医学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
Heliyon
·
2022
·
IF 3.776
Onyinyechi Lydia Ugorji,Ogochukwu Ngozi Chidimma Umeh,Chukwuma Obumneme Agubata,Dickson Adah,Nicholas Chinedu Obitte,Amarauche Chukwu
The formulation of niosomes is influenced by a number of variables, and these variables may eventually affect the formulation’s outcome. One of the elements that can influence the physico-chemical properties of niosomes is the method used in preparation of the formulation. In this study, we established if various methods of preparation have any impact on the prepared vesicles when loaded with 5-fluorouracil. Thereafter, a real-time cell assay (an in vitro cytotoxicity test) against HCT-116 colon cancer cell lines was done on an optimised batch. 5-fluorouracil loaded niosomes were prepared with either Tween 60 or Span 60 by four different methods - namely thin film hydration (TFH), reverse phase evaporation (RPE), evaporation/sonication (EVP/SON), and the ethanol injection method (EIM). In vitro evaluations were done on the formulations, and these included particle size analysis, entrapment efficiency, scanning electron microscopy (SEM), photomicrography, drug release, polydispersity index, and Fourier transform infrared spectroscopy (FTIR). The effects of the preparation method and type of non-ionic surfactants on encapsulation efficiency, particle size, and in vitro drug release of the niosomes at pH 7.4 were evaluated. An in vitro cytotoxicity test (real time cell assay (RTCA)) against HCT-116 cells was carried out using the optimised formulation. Results showed physically stable formulations. The TFH method produced the smallest particle sizes (187 nm and 482 nm), while the EVP/SON method produced the largest particle sizes (4476 nm and 9111 nm). The Tween-based niosomes prepared by TFH or RPE had higher drug entrapment. The FTIR studies of niosomal formulations showed broad peaks at wavenumbers above 3000 cm −1 , indicating strong hydrogen bonds. The RTCA showed 5-fluorouracil-loaded niosomes caused more sustained cell death compared to the pure drug and blank niosomes. The methods of preparation affected the particle size, polydispersity index, entrapment efficiency, and the physical stability of the vesicles. The thin film hydration method was more robust in the entrapped 5-fluorouracil and showed lower particle sizes when compared to all the other methods. RTCA showed sustained cell death in real time.
研究领域:
药剂学
·
肿瘤学
·
药物递送系统
·
细胞生物学
·
药物化学
✓ 已验证
DOI
PubMed
高影响因子
STARCH-STARKE
·
2017
·
IF 1.837
Dan-Ting Yin,Xin-Huai Zhao
The aim of this study was to assess the potential impact of 10 exogenous strains on acid production of maize resistant starch (RS) and xylo-oligosaccharides (XOS) during in vitro fermentation and on anti-colon cancer activities of fermentation products. Maize RS and XOS were fermented in vitro by the fecal extract from healthy adults in the absence and presence of one exogenous strain culture. Fermentation products were measured for their acetate, propionate, butyrate, and lactate contents, and growth inhibition on human colon carcinoma (HCT-116) cells. These strains in general conferred fermentation products with increased acetate, propionate, and butyrate contents, enhanced lactate conversion, and different growth inhibition on HCT-116 cells. Correlation analysis results demonstrated that butyrate and lactate contents of fermentation products made respective positive and negative contribution to the measured growth inhibition ( p < 0.05); however, acetate and propionate contents made an insignificant contribution to the measured growth inhibition ( p > 0.05). These results were mostly consistent with those from confirmation trials using standard acid solutions for the cells. It is highlighted that these strains had beneficial intervention in colonic fermentation of maize RS and XOS and anti-cancer activities of fermentation products, rendering lower lactate and higher butyrate contents and especially higher anti-colon cancer activities.
研究领域:
营养学
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微生物学
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癌症研究
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Shu-Fang Li,Teng-Gen Hu,Hong Wu
A colon-targeted delivery system that can efficiently deliver and release quercetin is essential to improve its bioavailability. We previously found that hydrophobic ethyl cellulose (EC) nanofibers could efficiently deliver quercetin to colon, but the release of quercetin was limited. To address this problem, hydrophilic gelatin (GN) was used as a regulator, and quercetin-loaded nanofibers with different mass ratios of EC to GN (3:1, 1:1, 1:2, 1:3) were fabricated by electrospinning. All nanofibers had a cylindrical morphology and high encapsulation efficiency (over 94 %), and there existed molecular interactions among quercetin, EC, and GN. The high GN content reduced the thermal stability of nanofibers but increased their surface wettability. Besides, these nanofibers had good stability in acidic and aqueous foods. Importantly, the release of quercetin in the simulated gastrointestinal fluid was <3 %. The addition of GN was beneficial to the release of quercetin in colon, and nanofibers with EC to GN being 1:3 had a more preferable release performance. The anticancer activity of nanofibers against HCT-116 cells was proved by inhibiting cell viability through the induction of apoptosis. Therefore, these nanofibers are potential carriers for efficient colon-targeted delivery of bioactive compounds in the food industry.
研究领域:
药学
·
生物医学工程
·
材料科学
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Heliyon
·
2024
·
IF 3.4
Onyinyechi Lydia Ugorji,Ikechukwu Virgilius Onyishi,Amarauche Chukwu,Anthony Amaechi Attama
5-flourouracil (5-FU) is typically modulated with leucovorin (LEU) in clinical practice to improve clinical efficacy and patient survival rates. However, this combination has undesirable side effects and makes 5-FU more toxic. Hence, integrating a vesicular system (proniosomes) with another delivery vehicle may improve drug targeting, while resolving the aforementioned drawbacks. This study aimed to engineer 5-FU/LEU proniosomes for possible delivery to the colon. The modified slurry approach was used to create drug-loaded proniosomes (150 mg/9 g of carrier) using both water-soluble (dextrin (DEX) and lactose (LAC)) and insoluble (Neusilin FH 2 (NEU)) carriers. The powdered formulations were filled into Eudragit S100 (10 %)-coated capsules or Eudragit FS 30D capsules for enteric- or colon-specific delivery. In vitro evaluations (flow properties, powder X-ray diffractometry (XRD) analysis, particle size analysis, entrapment efficiency, drug release, scanning electron microscopy (SEM), polydispersity index, Fourier transform infrared spectroscopy (FTIR), and stability studies) were performed on the formulations. An in vitro cytotoxicity test [real-time cell assay (RTCA)] against HCT-116 colon cancer cell lines was performed using the optimized formulation. In vitro evaluations showed that the nanoparticles had good physicochemical properties. RTCA studies showed sustained cell death with the formulations compared to the pure drug and placebo. The sequential drug release of the colon-targeted capsules containing 5-FU and LEU- loaded proniosomes showed negligible drug release in SGF (pH 1.2) and phosphate buffer solution (pH 6.8) (approximately 11 %) but profound drug release (>80 %) at pH 7.4. Drug-loaded proniosomes engineered for colon targeting (Eudragit S100 (10 %) capsules or Eudragit FS 30D capsules) showed good colon-specific targeting and favorable in vitro cytotoxicity profiles.
研究领域:
药剂学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Yuchen Feng,Siyi Lu,Qi Zhao,Tianyang Sun,Yuxi Shi,Guanyue Gao,Hao Wang,Jinfang Zhi
Carbon-based nanomaterials are viewed as preferred candidates as photothermal agents in tumor photothermal therapy due to their tailored structure and excellent biocompatibility. Herein, onion-like carbon (OLC) with controlled sp2/sp3-hybridized carbon ratios was explored as an effective photothermal reagent. The OLC was synthesized through thermal annealing of nanodiamonds, with an increase of the sp2 carbon content as the annealing temperature increased. The obtained OLC demonstrated high photothermal efficiency under 808 nm laser irradiation with a maximum value of 74.36% at an annealing temperature of 1400 °C. We suggest that a well-regulated amount of sp2 carbon in the OLC provided an abundance of conjugated π bonds, leading to enhanced light absorption and electron–phonon coupling. In addition, the OLC was conjugated with polyethylene glycol (PEG) and fluorescent molecules (FITC) to visualize the cellular uptake in HCT 116. In vivo tests demonstrated that the OLC-PEG-FITC exhibited good biocompatibility and achieved photothermal killing of cancer cells upon near-infrared laser irradiation. After treatment with the OLC-based photothermal agent, tumor tissues in tumor-bearing mice were effectively ablated. Taken together, these findings confirm the potential of the developed OLC as a photothermal agent for tumor therapy.
研究领域:
纳米技术
·
生物医学工程
·
材料科学
·
肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）