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STAR Protocols · 2024 · IF 1.3

Protocol for the cryopreservation of Cryptosporidium isolates using enteroids

Miner Deng,Yanting Wei,Na Li,Lihua Xiao,Yaoyu Feng,Yaqiong Guo

Summary A major bottleneck in the progress of Cryptosporidium research is the lack of accessible cryopreservation of Cryptosporidium oocysts. Here, we present a protocol for the cryopreservation of Cryptosporidium isolates using enteroids. We describe the steps for the establishment of enteroid cultures and cryopreservation of C. parvum -infected HCT-8 cultures. We then detail procedures for the recovery and propagation of frozen parasites using enteroids. For complete details on the use and execution of this protocol, please refer to Deng et al. 1

CAS / MDL: 145-42-6

研究领域: 微生物学 · 寄生虫学 · 细胞生物学

✓ 已验证产品关联 DOI PubMed 高影响因子

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JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH · 2021 ·Vol. 40 ·期 1 ·1-16 · IF 11.161

Dendrimer-modified gold nanorods as a platform for combinational gene therapy and photothermal therapy of tumors

Ye Lili,Chen Yaoming,Mao Jizong,Lei Xiaotian,Yang Qian,Cui Chunhui

Background The exploitation of novel nanomaterials combining diagnostic and therapeutic functionalities within one single nanoplatform is challenging for tumor theranostics. Methods We synthesized dendrimer-modified gold nanorods for combinational gene therapy and photothermal therapy (PTT) of colon cancer. Poly(amidoamine) dendrimers (PAMAM, G3) grafted gold nanorods were modified with GX1 peptide (a cyclic 7-mer peptide, CGNSNPKSC). The obtained Au NR@PAMAM-GX1 are proposed as a gene delivery vector to gene (FAM172A, regulates the proliferation and apoptosis of colon cancer cells) for the combination of photothermal therapy (PTT) and gene therapy of Colon cancer cells (HCT-8 cells). In addition, the CT imaging function of Au NR can provide imaging evidence for the diagnosis of colon cancer. Results The results display that Au NR@PAMAM-GX1 can specifically deliver FAM172A to cancer cells with excellent transfection efficiency. The HCT-8 cells treated with the Au NR@PAMAM-GX1/FAM172A under laser irradiation have a viability of 20.45%, which is much lower than the survival rate of other single-mode PTT treatment or single-mode gene therapy. Furthermore, animal experiment results confirm that Au NR@PAMAM-GX1/FAM172A complexes can achieve tumor thermal imaging, targeted CT imaging, PTT and gene therapy after tail vein injection. Conclusion Our findings demonstrate that the synthesized Au NR@PAMAM-GX1 offer a facile platform to exert antitumor and improve the diagnostic level of tumor.

CAS / MDL: 50-81-7 107-15-3 16903-35-8 57-09-0 96-33-3 2365-48-2 16940-66-2 7761-88-8

研究领域: 纳米技术 · 癌症治疗 · 生物医学工程 · 分子生物学

✓ 已验证产品关联 DOI PubMed 高影响因子

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Protocol for the cryopreservation of Cryptosporidium isolates using enteroids

Dendrimer-modified gold nanorods as a platform for combinational gene therapy and photothermal therapy of tumors