SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2017
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IF 2.536
Xu Tang,Juan Han,Yun Wang,Xu Bao,Liang Ni,Lei Wang,Longhua Li
A fluorescence probe has been designed and synthesized, and applied with a combined theoretical and experimental study. Research suggests that the probe can be used to sense Zn 2 + and Hg 2 + through selective turn-on fluorescence responses in the aqueous HEPES buffer (0.05M, pH = 7.4). The limit of detection (LOD) were determined as 1.46 × 10 − 7 M (Zn 2 + ) and 2.50 × 10 − 7 M (Hg 2 + ). Moreover, based on DFT, the geometry optimizations of probe 1, [1-Hg 2 + ] complex and [1-Zn 2 + ] complex were carried out using the Gaussian 09 program, in which the B3LYP function was used. The electronic properties of free probe 1 and the metal complexes were studied based on the Natural Bond Orbital (NBO) analyses. The probe 1 has also been successfully applied to detection of Zn 2 + and Hg 2 + in living cells.
研究领域:
化学
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生物化学
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材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2022
·
IF 4.601
Yuting Li,Luhan Wang,Minhua Qian,Shengda Qi,Lei Zhou,Qiaosheng Pu
ABSTRACT γ-Hydroxybutyric acid (GHB), a neurotransmitter or neuromodulator in the human central nervous system, is often abused in drug-facilitated sexual assaults due to its euphoric and sedative effects. While the analysis of GHB has received continuous attention, its inherent characteristics pose challenges. In the current study, capillary electrophoresis (CE) with capacitively coupled contactless conductivity detection (C 4 D) was built, and Good's buffers were evaluated as the background electrolytes for CE separation and C 4 D detection. On this basis, a simple and efficient CE-C 4 D method was developed for GHB analysis. Through theoretical discussion and experimental optimization, the separation of GHB and related positional isomers α-hydroxybutyric acid (AHB) and β-hydroxybutyric acid (BHB) was achieved within 4 min using 150 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) as the running buffer. Under the optimized condition, the relative standard deviations of migration time and peak area were less than 1.1% and 4.5%, indicating good precision. The C 4 D signal of GHB showed a good linear relationship with GHB concentration in the range of 3-300 µM with a determination coefficient of 0.9997, and the detection limit was calculated to be 0.37 µM based on the signal-to-noise ratio of three. Furthermore, liquid-liquid extraction (LLE) and solid-phase extraction (SPE) were comparatively studied for sample matrix purification. Combined with the optimized SPE procedure, the developed CE-C 4 D method has been successfully applied for the determination of exogenous GHB in spiked beverages and endogenous GHB in human urine.
研究领域:
分析化学
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法医学
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神经科学
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产品关联
DOI
PubMed
高影响因子
TALANTA
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2018
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IF 4.244
Li Yang,Yonghai Song,Mulan Zeng,Yue Du,Bingxian Peng,Zhenzhong Huang,Li Wang
It is of great significance for superoxide anion (O 2 •− ) detection due to its important role in regulation of biological function. The nanoscale coordination polymers (NCPs) core-shell nanostructures have attracted increasing interests for their nanosized-dependent properties. In this study, SiO 2 @Tb/guanosine 5′-monophosphate (GMP) NCPs with core-shell nanostructures was designed for O 2 •− detection. The SiO 2 @Tb/GMP NCPs core-shell nanostructures were produced by using SiO 2 nanoparticles (NPs) as template to control their nano-size. First, Tb 3+ was adsorbed on the surface of SiO 2 NPs by coordination with -COOH of SiO 2 NPs. Then the Tb/GMP NCPs shells were grown along the surface of SiO 2 NPs. The SiO 2 @Tb/GMP NCPs core-shell nanostructures were characterized with scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy and Zeta potential. The resulted SiO 2 @Tb/GMP NCPs core-shell nanostructures displayed strong Tb/GMP emission which decreased linearly with the increased O 2 •− concentration in the range from 5.3 nM to 6.0 μM with a limit detection of 2.18 nM. And the SiO 2 @Tb/GMP NCPs core-shell nanostructures also exhibited good selectivity and high sensitivity in HEPES buffer at pH 7.4.
研究领域:
材料科学
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分析化学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2019
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IF 3.206
Fuchu Gong,Wu Zou,Qinge Wang,Renxing Deng,Zhong Cao,Tingting Gu
Excited-state intramolecular proton transfer (ESIPT)-exhibiting polymer nanoparticles (e-PNPs) were prepared using 2-hydroxy-5-methylisophthalaldehyde and melamine as reactants. These e-PNPs possess abundant aromatic o -OH, C N and NH 2 groups, which are responsible for ESIPT modules and molecular recognition . Compared with small ESIPT compounds, e-PNPs displayed ultrabright luminescence and high fluorescence quantum yield owing to their rich ESIPT-chromophores and appropriate hydrophobic environment for ESIPT reaction. The e-PNPs can emit strong green fluorescence and exhibit typical dual emission in a HEPES buffer (20 mM, pH 7.5). Upon the addition of VB 1, the ESIPT reaction of e-PNPs was inhibited, eliciting obvious decrease in ESIPT fluorescence. By making use of this signaling mechanism, a tautomeric fluorescence quenching-based method for detecting VB 1 was established. A good linear range of 0.1–25 μM.L −1 was observed, bringing about an excellent detection limit of 2.6 nM.L −1 . Moreover, this assay shows good selectivity over other water-soluble vitamins and some biologically related species. The employment of e-PNPs for the determination of VB 1 in kiwi and grape juice samples showed remarkable abilities in terms of the simplicity, stability, reproducibility and sensitivity, which conforms its great potential for real sample analysis. Therefore, e-PNPs can offer a simple, highly sensitive and selective sensing model for fluorescence detection of VB 1 .
研究领域:
材料科学
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分析化学
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纳米技术
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生物技术
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Lianshun Zhang,Mengqing Jia,Xi Wang,Lei Gao,Bo Zhang,Lei Wang,Jinming Kong,Lianzhi Li
It is of great significance to sensitively and selectively detect uranyl ion (UO 2 2+ ) in environmental and biological samples due to the high risks of UO 2 2+ to human health. However, such suitable sensors are still scarce. A novel fluorescence sensor based on a dansyl-modified peptide, Dansyl-Glu-Glu-Pro-Glu-Trp-COOH (D-P5), was efficiently synthesized by Fmoc solid phase peptide synthesis. As the first linear peptide-based fluorescence sensor for UO 2 2+ , D-P5 exhibited high selectivity and sensitivity to UO 2 2+ over 27 metal ions (UO 2 2+ , Cr 3+ , Cu 2+ , Ba 2+ , Hg 2+ , Pb 2+ , Co 2+ , Ag + , Fe 3+ , Ca 2+ , K + , Mg 2+ , Mn 2+ , Na + , Ni 2+ , Cd 2+ , Zn 2+ , Al 3+ , Dy 3+ , Er 3+ , Gd 2+ , Ho 3+ , La 3+ , Lu 3+ , Pr 3+ , Sm 3+ , Tm 3+ ) by a turn-off fluorescence response in 10 mM HEPES buffer (pH 6.3). The effects of anions such as S 2− , NO 3 – , SO 4 2− CO 3 2– , HCOO – , antioxidant ascorbic acid and 4-nitrophenyl acetate on the selectivity for UO 2 2+ detection were also studies. D-P5 sensor could be used for detecting UO 2 2+ in a good linear relationship with concentration in the range of 0–8.0 μM with a low limit of detection of 83.2 nM. Furthermore, the interaction of the sensor with UO 2 2+ was characterized by ESI-MS, IR, XPS and ITC measurements. The 1:1 binding stoichiometry between the sensor and UO 2 2+ was measured by the job’s plot and further verified by ESI-MS. The binding constant of the sensor with UO 2 2+ was calculated to be 9.8 × 10 4 M −1 by modified Benesi-Hildebrand equation. ITC results showed that the ΔH θ and ΔS θ for the interaction of D-P5 with UO 2 2+ were −(7.167 ± 1.25) kJ·mol −1 and 66.5 J·mol −1 ·K −1 , respectively. Time-resolved fluorescence spectroscopy indicated that the mechanism of fluorescence quenching of D-P5 by UO 2 2+ ion was static quenching process. In addition, this sensor displayed a good practicality for UO 2 2+ detection in lake water sample without tedious sample pretreatment.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2020
·Vol. 12
·期 7
·943-950
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IF 2.596
Dandan Li,Lihua Gong,Zhenyang Qiao,Congcong Zhu,Qiaojuan Jia,Huajie Kong,Shuyan Jiao
A novel highly selective colorimetric fluorescence probe, POPH, has been successfully synthesized and it shows remarkable selective and sensitive response to Cu2+ in aqueous media (HEPES buffer, pH = 7.4). The fluorescence of probe POPH immediately decreased when 1.1 equiv. of Cu2+ was added to a solution of probe POPH, accompanied by color change from wathet blue to colorless under 365 nm UV lamp illumination. The possible bonding model of probe POPH with Cu2+ was examined through HRMS, FESEM, TEM, FTIR spectroscopy and DFT calculations. Moreover, the probe POPH could be used for monitoring Cu2+ in living cells using laser scanning confocal microscopy.
研究领域:
分析化学
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材料科学
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生物化学
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理论化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）