SEPARATION AND PURIFICATION TECHNOLOGY
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2024
·
IF 8.1
Jiachang Shen,Maohua Yang,Jianmin Xing
Utilization of an in vitro multi-enzyme catalytic system for the synthesis of fructose from carbon dioxide is a promising approach towards achieving carbon neutrality. However, the lack of effective separation methods for the buffer HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and the product fructose within the system has hindered the industrialization of in vitro multi-enzyme catalytic synthesis systems. This study aimed to separate and recover HEPES and fructose using nanofiltration technology. We investigated the influence of various parameters, including total solution concentration, solute concentration ratio, pH, and temperature, on the nanofiltration separation performance. Experimental results revealed that increasing the pH to 9.0 significantly decreased the fructose retention rate while maintaining a nearly complete HEPES retention rate. The HEPES-fructose mixed solution was further subjected to diafiltration using a spiral-wound membrane, ultimately yielding fructose and HEPES solutions with purities of 90.22 % and 92.93 %, respectively. Combining separation test results with models such as DSPM-DE, and using characterization methods such as ATR-FTIR and pore size distribution analysis, we observed pore swelling during nanofiltration of the HEPES-fructose solution at pH 9.0. A mechanism for this pore swelling, induced by the dissociation of HEPES under the influence of pH, was proposed: Dissociated HEPES in the solution interacts with the membrane surface at the liquid-membrane interface, causing reversible swelling of the membrane pores at the membrane surface, thereby reducing the retention rate of fructose. The nanofiltration process developed in this study effectively separates HEPES and fructose, enabling resource recycling and cost reduction. Furthermore, this study provides theoretical foundations and practical guidance for the design of nanofiltration separation strategies for similar systems using HEPES as a buffer, holding significant scientific and practical value.
研究领域:
生物工程
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环境科学
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产品关联
DOI
高影响因子
ADVANCED POWDER TECHNOLOGY
·
2017
·
IF 2.659
Shilei Wang,Qin Li,Fengxi Chen,Jun Ke,Rong Chen
Owing to the strongly morphology and size-dependent properties of nanomaterials, developing a simple and eco-friendly approach for controlling synthesis and assembly of hierarchical CuO nanostructures plays a critical role. In this work, we successfully developed an eco-friendly and facile hydrothermal route for controllable synthesis of various morphologies CuO nanostructures, where traditional and toxic additives were replaced by biocompatible 4-(2-hydroxyethyl)-1-piperazineethane-sulfonic acid (HEPES). The growth rate and orientation of CuO nanoparticles were tuned through adjusting the concentration and pH value of HEPES. The XRD pattern, SEM, UV-DRS, and BET indicated CuO nanostructures maintained high purity and excellent crystalline nature and showed more regular morphology and size with enhanced visible light absorption. The analogous photo-Fenton assay demonstrated that the as-prepared CuO nanostructures present enhanced dye removal capability when compared with CuO synthesized in pure water. Furthermore, the antibacterial assay on S. aureus was also investigated, and CuO nanoparticles with smallest size displayed the best antibacterial performance. These results reveal that HEPES shows a remarkable influence on the possible application of as-prepared CuO nanostructures which having great potential in pollutant removal.
研究领域:
材料科学
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环境科学
·
生物学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Lin Fan,Pengfei Wang,Mingyue Wang,Wanhong Lü,Fengyou Wang,Huilian Liu,Jinghai Yang,Lili Yang
In the development of high-efficiency n-i-p planar heterojunction perovskite solar cells (PSCs), the development of dense electron-transporting layers (ETLs) with excellent electrical properties and hetero-interface quality is very important for promoting perovskite growth and carrier transport dynamics. Herein, we propose a simple and effective strategy to simultaneously improve the electronic properties and interface quality of a tin oxide (SnO 2 ) ETL by introducing 2-hydroxyethyl modified gold nanostars (HEPES-Au NSs) into a commercial SnO 2 colloidal dispersion in order to synthesize mixed ETLs at low temperatures (≤150 ℃). Based on the unique plasmonic structure of the HEPES-Au NSs, we demonstrate that the modified ETLs exhibit higher conductivity and greater efficiency in the extraction, transfer, and collection of photogenerated electrons than conventional SnO 2 ETLs. Moreover, the chemical bond interaction between HEPES-Au NSs and the perovskite enhances the affinity of the SnO 2 /perovskite hetero-interface, which effectively improves the nucleation and crystallization kinetics of the perovskite, thus producing a high-quality absorber with high crystallinity and superior absorbance. Meanwhile, the 2-hydroxyethyl (HEPES) adsorbed onto the SnO 2 surface effectively passivates perovskite-related trap states, suppressing the non-radiative recombination and leakage current, and ultimately improving the relative electronic properties and photovoltaic performance output of the modified device. The results show that the power conversion efficiency (PCE) of the modified PSCs is significantly better than that of the original SnO 2 -based planar devices, and the optimal PCE reached 21.13%, with negligible hysteresis. Additionally, owing to the multifunctional effects of SnO 2 modified by HEPES-Au NSs, the functionalized devices without encapsulation also demonstrate reliable reproducibility and good stability, which shows great advantages in the development of high-efficiency flexible PSCs and monolithic tandem devices.
研究领域:
材料科学
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能源科学
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光电转换
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产品关联
DOI
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
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2011
·Vol. 11
·期 9
·7794-7801
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IF 1.352
Zhang Wei,Wang Qiao,Qin Fan,Zhou Huamin,Lu Zhong,Chen Rong
Palladium nanoparticles (NPs) were successfully synthesized via a rapid and facile microwave route in HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid) buffer solution. The shape- and size-controlled Pd nanoparticles could be obtained by one-step method without dependence
of seed-mediated growth. The capping agent plays a key role in the formation of Pd NPs with different shape and size, which could be tuned by varying capping agents such as polyvinylpyrrolidone (PVP), cetyltrimethylammonium bromide (CTAB), sodium citrate (Na3(cit)) and potassium
bromide (KBr). The size-dependent catalytic activities of the obtained Pd NPs for Suzuki coupling reaction were also investigated. It demonstrated that the catalytic activity of Pd NPs was enhanced regularly with the decrease of particle size. Pd NPs less than 10 nm exhibited better catalytic
activities for Suzuki reaction than the commercial Pd/C catalyst. Pd/MWCNTs and Pd/SBA-15 nanocomposites were also prepared by a facile method and afforded good catalytic activity and reusability. This "green" synthetic protocol could be used as a general method for the rapid synthesis of
transition metal nanoparticles.
研究领域:
化学
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材料科学
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催化反应
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DOI
PubMed
高影响因子
ChemistrySelect
·
2023
·Vol. 8
·期 5
·e202204363
·
IF 2.1
Tingting Xu,Panpan Xu,Guilin Xu,Mengyao Liu,Yinggui Zhu
Graphical In the study, Ru(bpy) 3 2+ was used as the luminophore, HEPES buffer was served as the co-reactant, and ATP was added to the system to amplify the luminous signal. It was speculated that ATP acts as an co-reaction accelerator to promote the oxidation of HEPES to HEPES + ⋅, more HEPES⋅ can be generated, which then reacts with oxidized Ru(bpy) 3 3+ to emit stronger ECL signals. Moreover, I - consumes ATP, Ru(bpy) 3 2+ , and HEPES, leading to a decrease in ECL signal. Adenosine triphosphate is an important biological molecule that provides energy and substrate for various cellular biochemical processes,it's ubiquitous in our lives. In this work, a novel electrochemiluminescence (ECL) sensor composed of Ru(bpy) 3 2+ , 2-[4-(2-hydroxyethyl)piperazin-1-yl] ethanesulfonic acid (HEPES) and adenosine 5’-triphosphate disodium salt (ATP) was developed for the first time. In this ECL system, Ru(bpy) 3 2+ was used as the luminophore, HEPES buffer was served as the co-reactant, and ATP was added to the system to amplify the luminous signal to investigate the ECL behavior. Through fluorescence spectroscopy, ECL spectroscopy, and cyclic voltammetry (CV), it can be hypothesized that the possible ECL mechanism is that ATP acts as an advanced co-reaction promoter to accelerate the oxidation of HEPES to HEPES + ⋅, more HEPES⋅ can be generated, which then reacts with oxidized Ru(bpy) 3 3+ to emit stronger ECL signals. Subsequently, the influence of the ATP concentrations and the CV scanning speed was investaged. Moreover, it was observed that I − could quench the ECL intensity, the linear relationship range of I − concentration is from 10 −3 to 10 −6 m , the limit of detection is 3.7×10 −8 m (signal/noise=3), therefore, a new, highly sensitive, and selective ECL approach for I − detection was created.
研究领域:
分析化学
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生物化学
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产品关联
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
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2013
·
IF 2.39
Qin Li,Hui Li,Runming Wang,Guangfang Li,Hao Yang,Rong Chen
Size- and morphology-controlled zinc oxide (ZnO) micro-/nanostructures have been successfully synthesized via a facile and rapid microwave and ultrasonic wave combined method in HEPES solution (HEPES = 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid). The as-prepared ZnO products are characterized by powder X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED) and UV–vis diffuse reflection spectroscopy (DRS). Various morphologies of ZnO products, including grenade-like, column-like, spindle-like, rod-like, shuttle-like and flower-like micro-/nanostructures are obtained, which are strongly dependent on Zn/HEPES moral ratio, pH value and Zn precursor. It is found that HEPES plays a crucial role in the formation of ZnO micro-/nanostructures with controllable size and morphology. The photocatalytic activities of the prepared ZnO micro-/nanostructures are evaluated by degradation of methylene blue (MB) under UV light irradiation, among which spindle-like ZnO microstructures exhibit superior photocatalytic activity compared with other ZnO products.
研究领域:
材料科学
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化学工程
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Yaqi Qu,Hualiang An,Xinqiang Zhao,Yanji Wang
High dispersion of active metal is an inevitable requirement to improve the catalytic performance of supported metal catalysts and achieving this goal always requires the support with a large specific surface area. Here we innovatively prepared a ZrO 2 @Pt catalyst using ZrO 2 with a small specific surface area as the core which is coated with highly dispersed Pt nanoclusters (<2 nm). In this process, the surface of ZrO 2 was firstly functionalized to increase the number of hydroxyl group, Pt nanoclusters were formed simultaneously and uniformly coated on the ZrO 2 surface. In this work, much attention was paid to the role of 2-[4-(2-hydroxyethyl)-1-piperazinyl] ethanesulfonic acid (HEPES) and ethanol in the one-pot preparation process. The results showed that HEPES acted as a reductant, while treatment with ethanol promoted both the generation of hydroxyl groups on ZrO 2 surface and the reduction of Pt precursor under mild conditions. As-prepared ZrO 2 @Pt catalyst showed excellent catalytic performance in the selective hydrogenation of nitrobenzene; nitrobenzene was completely converted to aniline with 100 % selectivity at room temperature and 8 bar H 2 pressure within 30 min, and the TOF reached as high as 3304 h −1 . Moreover, ZrO 2 @Pt also exhibited a good reusability and a broad substrate applicability. The results of both DRIFTS analysis and DFT calculation demonstrated that the hydrogenation of nitrobenzene on ZrO 2 @Pt catalyst followed the condensation reaction pathway. Our findings can provide a new approach to obtaining highly dispersed metal nanoparticles on the support with a small specific surface area.
研究领域:
催化
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材料科学
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化学工程
·
物理化学
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产品关联
DOI
高影响因子
GREEN CHEMISTRY
·
2025
·
IF 9.2
Ying Liu,Xiangjin Liang,Jun Lu,Yapeng Chen,Jun Liu,Baoying Wang,Ruixue Ma,Junchen Xu,Jun Cheng
How to utilize low-cost easily-acidified ammonium sulfate, a byproduct of coking industry, to farm microalgae for CO2 fixation is a difficult problem. Ammonium sulfate was mixed with sodium nitrate to cultivate Nannochloropsis oceanica at stable pH using HEPES-NaOH for solving acidification problem, and downregulate assimilatory nitrate reduction to save energy for improving carbon sequestration. Transcriptomic sequencing showed that nitrate assimilation consumed much ATP and reductivity derived from photosystem I, and competed with Calvin cycle for energy to limit CO2 fixation rate of microalgae in conventional nitrate medium. However, pure ammonium culture attenuated GS/GOGAT cycle (a main pathway for ammonium assimilation and amino acids synthesis) to limit nitrogen utilization rate of microalgal cells. In the mixed ammonium and nitrate supply modes, nitrogen metabolic pathway was down-regulated, while the Calvin cycle pathway was up-regulated to improve CO2 fixation. Nitrate reduced the extent of up-regulation of the ammonium transporter to prolong effective utilization time of ammonium. Asparagine synthetase activity in accessorial pathway of nitrogen assimilation substantially increased, while asparaginase was down-regulated to avoid harmful overaccumulation of ammonium in the cell. Down-regulation of tricarboxylic acid cycle attenuated intermediate 2-oxoglutarate shift to ammonium assimilation and improved efficiency of nitrogen utilization. The optimum condition for microalgal growth was at total nitrogen concentration of 5.28 mM and a nitrate to ammonium molar ratio of 2:3. Compared with the control (2.64 mM nitrate), the maximum photosynthetic electron transfer efficiency rETR increased by 34.9%, chlorophyll a concentration increased by 110% and average biomass growth rate increased by 33.7%.
研究领域:
微藻生物技术
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环境生物技术
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植物生理学
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生物信息学
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海洋生物技术
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2020
·Vol. 12
·期 41
·4996-5003
·
IF 2.596
Jingzhou Hou,Zhengfan Shui,Jiawei Li,Danqun Huo,Xiaohui Tang,Chenhui Yu,Qiang He,Cailin Qiao
Different valence states of chromium ions possess huge differences in toxicity. Hence, it is an innovative idea to design a reasonable probe to detect Cr according to the toxicity characteristics of different valence states. We report a novel, rapid, simple and accurate probe for the detection of Cr3+ and Cr6+ ions. As a probe, gold nanoparticles (Au NPs) are successfully modified using tartaric acid (TA) and 2-[4-(2-hydroxyethyl)-1-piperazinyl] ethanesulfonic acid (HEPES) via a two-step modification; the probe shows an increase in the sensitivity towards Cr6+ and decreases towards Cr3+, which is consistent with their toxicity characteristics, benefiting the assessment of total Cr toxicity. The proposed probe achieves considerable two-channel (ultraviolet absorption spectrum and naked eye vision) detection of Cr3+ and Cr6+ providing wide linearity regions and low detection limits. Meanwhile, the results of the interference experiments and analysis of the real samples showed high selectivity and accuracy of the proposed method. With popularization, this method possesses great potential in environmental monitoring and control.
研究领域:
环境科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2021
·
IF 4.089
Weiming Ji,Shunni Dong,Wei Fan,Chao Lv,Jingjing Nie,Binyang Du
A functional monomer, namely, 1-hexene-4-(benzal-2,2′:6′,2″-terpyridines)pyridinium bromide (HTP) was synthesized and incorporated into the cross-linking network of microgels. The HTP-functionalized microgels (HTP-MGs) were synthesized by surfactant-free emulsion copolymerization with N-isopropylacrylamide as the main monomer, 1-vinylimidazole as a quaternization cross-linkable comonomer, HTP as the functional comonomer, and 1,6-dibromhexane as the quaternization agent. The obtained HTP-MGs were spherical with a hydrodynamic radius Rh of 205 ± 2 nm and uniform size distribution in HEPES buffer aqueous solutions at 25 °C. The HTP-MGs exhibited a characteristic UV–vis absorption peak at 315 nm and a characteristic fluorescent emission peak at 430 nm, respectively. The HTP-MGs can selectively, sensitively, and optically respond to Fe2+ ions in aqueous solution with a fast response time of 3 s in wide pH (2–10) and temperature (25–50 °C) ranges, resulting in a color change in HTP-MG HEPES buffer aqueous solutions from colorless to purplish red, which can be easily distinguished by naked eyes. A linear concentration range of 0–14 μM and a detection limit (DL) of 82 nM were achieved for detecting Fe2+ ions in HEPES buffer aqueous solutions (pH = 7) at 25 °C. The molar binding ratio of the HTP moiety within HTP-MGs to Fe2+ ions was 2:1, as revealed by Job’s plot. It was found that the coexistence of 15 species of other metal ions except of Cu2+ ions studied here did not affect the detection of Fe2+ ions. Furthermore, a fluorescent emission peak at 371 nm with strongly enhanced fluorescent intensity was observed for HTP-MG HEPES buffer aqueous solutions with the addition of Zn2+ or Cd2+ ions. By combining the optical response to Fe2+ ions and fluorescent response to Zn2+ ions, it was demonstrated that HTP-MGs could also have potential applications in the fields of encryption and anti-counterfeiting.
研究领域:
材料科学
·
分析化学
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DOI
高影响因子
RSC Advances
·
2013
·Vol. 3
·期 26
·10355-10362
·
IF 2.562
Ai-Jun Wang,Fang-Fang Li,Jie-Ning Zheng,Huan-Xiang Xi,Zi-Yan Meng,Jiu-Ju Feng
Using a Good's buffers, N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid (HEPES) both as reducing and shape-directing agent, porous Pd nanoflowers were facilely prepared with high-yield by one-pot chemical reduction method, which are preferentially growing along the (111) directions, without any seed or surfactant. It is found that the amount of the precursors, temperature and reaction time play important roles in this synthetic procedure. And the flower-like Pd nanostructures exhibit excellent electrocatalytic activity towards methanol oxidation in alkaline media with good stability, which show potential applications as an effective catalyst in direct methanol fuel cell.
研究领域:
材料科学
·
电化学
·
纳米技术
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2017
·
IF 2.536
Xu Tang,Juan Han,Yun Wang,Xu Bao,Liang Ni,Lei Wang,Longhua Li
A fluorescence probe has been designed and synthesized, and applied with a combined theoretical and experimental study. Research suggests that the probe can be used to sense Zn 2 + and Hg 2 + through selective turn-on fluorescence responses in the aqueous HEPES buffer (0.05M, pH = 7.4). The limit of detection (LOD) were determined as 1.46 × 10 − 7 M (Zn 2 + ) and 2.50 × 10 − 7 M (Hg 2 + ). Moreover, based on DFT, the geometry optimizations of probe 1, [1-Hg 2 + ] complex and [1-Zn 2 + ] complex were carried out using the Gaussian 09 program, in which the B3LYP function was used. The electronic properties of free probe 1 and the metal complexes were studied based on the Natural Bond Orbital (NBO) analyses. The probe 1 has also been successfully applied to detection of Zn 2 + and Hg 2 + in living cells.
研究领域:
化学
·
生物化学
·
材料科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）