An electrochemical sensing interface is limited by poor reproducibility and inevitable interferences present in practical applications due to the weak electrochemical signals of nanotags. This motivates the need for effective strategies to enhance the electroactivity performances of nanotags. In this contribution, a plasmon-enhanced electroactivity mechanism is proposed for AuRu-based nanostructures under illumination and applied for accurate detection of human epidermal growth factor receptor-2 (HER2). AuRu nanoparticles (NPs) harvested light energy through plasmon excitation and generated holes to participate in the electrooxidation process. The production of holes resulted in the electrooxidation signal enhancement of AuRu NPs. AuRu NPs were assembled with Au NPs using HER2 aptamers as linkers, and the plasmonic coupling between AuRu NPs and Au NPs produced an intense electromagnetic field, which further enhanced the electrooxidation signals of AuRu NPs. An AuRu–Au NP assembly-dependent electrochemical aptasensor was established for the accurate detection of HER2, and the limit of detection (LOD) was as low as 1.7 pg/mL. The plasmon-enhanced electroactivity mechanism endowed AuRu-based nanostructures with strong and noninterfering electrochemical signals for sensitive and accurate detection. This insight opens new horizons for the construction of desired electroactive nanostructures for electroanalysis applications.
Yawen Wu,Xiaolong Chen,Changjun Hou,Peng Lu,Lan Peng,Jiawei Li,Mei Yang,Danqun Huo
Human epidermal growth factor receptor 2 (HER2) is one of the specific markers of breast cancer, which is of great significance to the early diagnosis and prognosis of breast cancer. Here, a fluorescence biosensor was established to detect HER2 based on the fluorescence resonance energy transfer (FRET) and photoinduced electron transfer (PET) occurring between the bimetal-polydopamine organic framework with core–shell structure Au@PDA@UiO-66 and the Cy5 fluorophore in HER2-Cy5-Apt. Au@PDA@UiO-66 owns high-efficiency fluorescence quenching ability due to its large specific surface area and strong adsorption of single-stranded DNA. When the target appears, the fluorescence recovery space mediated by the target is large, so the proposed biosensor has better sensitivity in theory. Under optimized conditions, the proposed fluorescent biosensor can detect HER2 in a range of 0.005 ng mL−1 to 15 ng mL−1, with an actual detection limit as low as 0.005 ng mL−1. Corresponding selective experiments, reproducible experiments, and spiked experiments performed well, showing its great potential in HER2 detection.
危险品化学品经营许可证（带存储）