BIOCONJUGATE CHEMISTRY
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2020
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IF 4.031
Yingshu Guo,Fei Liu,Yinhua Hu,Xiaofei Zheng,Xiuping Cao,Yanxi Zhu,Xiaoru Zhang,Dongjiao Li,Zhenhua Zhang,Si-kai Chen
Dark-field microscopy (DFM) based on localized surface plasmon resonance (LSPR) was used for observation of experimental phenomena, which is a hopeful nondamaging and non-photobleaching biological imaging technique. In this strategy, plasma nanoaggregates with stronger scattering efficiency were formed in the presence of the target, causing a “turn-on” phenomenon, when asymmetry modified AuNPs were introduced as probes with zero LSPR background. First, Au1–N3 probe and Au2–C≡C probe were designed for the cycloaddition between azide and alkyne to form AuNP dimers under catalytic action by Cu+, which was obtained from the reduction of Cu2+ by sodium ascorbate. The two kinds of probes were successfully used for the detection of Cu2+ in rat serum. Then, to apply this concept to protein on cells, DNA and antibody were modified on the probes. DNA1/Au1–N3 probe and anti-HER2/Au2–C≡C probe were proposed for HER2 protein DFM on cells. By designing an aptamer sequence in primer, the rolling circle amplification (RCA) was introduced in HER2 DFM on cells, and the image signal was much brighter than that from no-RCA. The unique design made it easier to discriminate the target signal from background noise in cell DFM. This method might be used in the fields of molecular diagnostics and cell imaging.
研究领域:
生物化学
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分子生物学
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医学影像学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2018
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IF 5.123
Hong Xing,Tianxiang Wei,Xin Lin,Zhihui Dai
An enhanced near-infrared (NIR) FRET biosensing system based on the novel donor-acceptor pair (MnCuInS/ [email protected] and urchin-like Au NPs) was developed for the sensitive detection of HER2 protein. In this strategy, MnCuInS/ZnS quantum dots (QDs) were chosen due to their NIR emission, high quantum efficiency , and low toxicity. Amounts of MnCuInS/ZnS QDs were encapsulated in BSA to form MnCuInS/ [email protected] nanoparticles, which can easily transfer MnCuInS/ZnS QDs from organic phase into aqueous solution, and the formed nanoparticles were applied as the energy donor which significantly enhanced the fluorescence intensity . As for the energy acceptor, urchin-like Au nanoparticles (Au NPs) with novel structure, good stability and excellent quenching ability towards NIR fluorescence were selected. Thus, the NIR MnCuInS/ [email protected] and urchin-like Au NPs were designed as a novel donor-acceptor pair for FRET assay. By combining the optical advantages of NIR QDs encapsulated BSA nanoparticles with the excellent fluorescence quenching ability of urchin-like Au NPs, the proposed FRET-based biosensor realized enhanced FRET effect for highly sensitive detection of HER2 in human serum samples. A wide detection range (2–100 ng mL −1 ) and a low detection limit (1 ng mL −1 ) were obtained. This sensing system can decrease the interference of other biomolecules in NIR region, can be applied to other biomarker determination in vitro, and also shows great potential for in vivo diagnosis.
研究领域:
生物传感
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医学检测
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纳米材料科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2024
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IF 6.7
Qianxi Zhou,Yuying Pan,Qizhi Liang,Jiahua Yang,Sha Zhu,Hai Shi,Genxi Li
Peptide-engineered nanoparticles have great potential for biomedical research and application. In this work, we have designed and fabricated an electrochemical biosensor based on peptide-guided assembly of silver nanoparticles (AgNPs), in which a peptide is endowed with dual functions to recognize target and guide assembly of AgNPs. As a proof of concept, the performance of this biosensor is validated by quantifying human epidermal growth factor receptor 2 (HER2) protein. In detail, the end of the HER2-specific binding peptide is grafted with a positively charged peptide, which can guide the orderly assembly of AgNPs, while electrochemical signals can be obtained through phosphine-silver coordination. Using this electrochemical biosensor, HER2 protein can be quantified with high sensitivity and specificity, and the limit of detection can be as low as 0.05 pg/mL. Moreover, the antifouling electrode surface prepared by the modification of a layer of antifouling zwitterionic peptide allows this biosensor to be used for the detection of serum HER2 protein from breast cancer patients, which provides the clear evidence for the distinction of HER2+ breast cancer patients and HER2- breast cancer patients.
研究领域:
生物医学工程
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纳米技术
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癌症诊断
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2023
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IF 6.2
Xiaoxian Liu,Yifan Zhu,Caoxin Li,Yanyun Fang,Jinna Chen,Fei Xu,Yanqing Lu,Perry Ping Shum,Ying Liu,Guanghui Wang
Accurate and ultrasensitive evaluation of human epidermal growth factor receptor 2 (HER2) protein is key to early diagnosis and subtype differentiation of breast cancer. Single-cell analyses to reduce ineffective targeted therapies due to breast cancer heterogeneity and improve patient survival remain challenging. Herein, we reported a novel droplet microfluidic combined with an instant cation exchange signal amplification strategy for quantitative analysis of HER2 protein expression on single cells. In the 160 μm droplets produced by a tapered capillary bundle, abundant Immuno-CdS labeled on HER2-positive cells were replaced by Ag + to obtain Cd 2+ that stimulated Rhod-5N fluorescence. This uniformly distributed and instantaneous fluorescence amplification strategy in droplets improves sensitivity and reduces signal fluctuation. Using HER2 modified PS microsphere to simulate single cells, we obtained a linear fitting of HER2-modified concentration and fluorescence intensity in microdroplets with the limit detection of 11.372 pg mL −1 . Moreover, the relative standard deviation (RSD) was 4.2-fold lower than the traditional immunofluorescence technique (2.89% vs 12.21%). The HER2 protein on SK-BR-3 cells encapsulated in droplets was subsequently quantified, ranging from 9862.954 pg mL −1 and 205.26 pg mL −1 , equivalent to 9.795 × 10 6 and 2.038 × 10 5 protein molecules . This detection system provides a universal platform for single-cell sensitive quantitative analysis and contributes to the evaluation of HER2-positive tumors.
研究领域:
生物技术
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医学诊断
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癌症研究
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微流控
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）