Ziyu Song,Jiajun Wang,Shaojie Tan,Jing Gao,Lu Wang
The treatment of severely damaged wounds is a serious challenge as these wounds have poor self-healing capacity, take longer to heal, and are prone to becoming chronic wounds. To solve this problem, PCL/gelatin electrospinning nanofiber membranes were combined with rGO-loaded chitosan non-woven fabrics to prepare biomimetic bilayer skin scaffolds. Among them, the PCL/gelatin nanofiber membrane was used to simulate the epidermis layer with a dense structure, and the chitosan non-woven fabric was used to simulate the dermis layer with a loose structure. When the concentration of the spinning solution was 7 %, and the average fiber diameter was 241 nm, HaCaTs had the best cell proliferation and adhesion, and the re-epithelialization process was fast. To enhance the induction effect of scaffolds on dermal cells, the electroactive substance rGO was used to give the scaffold electrical conductivity . Considering the biocompatibility, the concentration of GO dispersion was controlled at 1 mg/mL, and the obtained [email protected] 1rGO scaffolds had high conductivity, as well as the improved mitochondrial membrane potential of HFF-1 s which increased cell viability . So the induction of HFF-1 s migration was obvious. After cross-linking composite scaffolds with glutaraldehyde , the scaffolds still showed good cytocompatibility, at the same time, the peel strength was increased from 7.21 cN to 350 cN, and the structural stability of the nanofiber membrane layer in the liquid environment was also enhanced. Both the moisture absorption and moisture permeability of composite scaffolds were improved, which could maintain the moist environment of the wound to a certain extent. Altogether, results showed that electroactive biomimetic bilayer fibrous scaffolds have great application potential in promoting skin tissue regeneration.
Jingyuan Ji,Chenjia Zhao,Chen Hua,Lu Lu,Yuan Pang,Wei Sun
As attempting personalized medicine, 3D-printed tissue engineering scaffolds are employed to combine with therapeutic proteins/peptides especially in the clinical treatment of infectious diseases, genetic diseases, and cancers. However, current drug-loading methods, such as immersion and encapsulation, usually lead to the burst release of the drugs. To address these issues, we proposed an integrated strategy toward the long-term controlled release of protein. In this study, patient-customized 3D scaffolds incorporated with drug-loaded microspheres were printed to realize the effective delivery of the anti-human papillomavirus (anti-HPV) protein after cervical conization in the treatment of cervical cancer. The 3D-printed scaffold could provide mechanical support to the defect site and ensure local release of the drug to avoid systemic administration. Meanwhile, microspheres serve as functional components to prevent the inactivation of proteins, as well as regulate their release period to meet the time requirement of different treatment courses. The research also utilized bovine serum albumin as a model protein to validate the feasibility of these scaffolds as a generic technology platform. The bioactivity of the released anti-HPV protein was validated using a pseudovirus model, which demonstrated that the microsphere encapsulation would not cause protein denaturation during the scaffold fabrication process. Besides, 3D-printed scaffolds incorporated with carboxylated chitosan microspheres were biocompatible and beneficial for cell attachment, which have been demonstrated by favorable cell viability and better coverage results for HeLa and HFF-1. This study highlights the great potential of scaffolds incorporated with microspheres to serve as tissue engineering candidate products with the function of effective protein delivery in a long-term controlled manner and personalized shapes for clinical trials.
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