Zhenyu Wu,Zhiwei Ding,Yue Li,Shakeel Zeb,Weibai Bian,Yinhua Jiang,Yan Liu
The efficient capture of myricetin is often challenged by its instability in alkaline environments, which contradicts the alkaline conditions required for traditional boronate affinity materials. To address this, a dual-recognition molecularly imprinted polymer (DMIP) supported on HOF-101 was developed using a deep eutectic solvent (DES)-driven strategy. A dual DES system was employed to achieve synergistic recognition: ternary DES 1 acts as a functional monomer combining boronate affinity and hydrogen bonding, while binary DES 2 serves as a green solvent and porogen. Crucially, the ethylene glycol in DES 2 facilitates “teamed boronate affinity” via B–O coordination, significantly lowering the p K a of boronic acid groups. This mechanism enables efficient specific recognition at pH 6.0, effectively overcoming the limitation of decreased affinity under weakly acidic conditions. The optimized BA-DES/DMIPs exhibited rapid equilibrium (20 min), a high Langmuir maximum adsorption capacity (45.016 mg/g), and excellent stability over five cycles. Furthermore, the material demonstrated superior selectivity against structural analogs and achieved satisfactory recovery rates (99.80%–101.67%) in grape juice, confirming its potential for green and efficient separation in complex matrices.
Liying Huang,Shiwei Xu,Yueyue Feng,Guofeng Wang,Qian Shi,Min Zhang,Ying Liu,Nannan Liu
The fluctuation of copper ion (Cu 2+ ) content is closely related to the physiological state of the human body. Developing a facile and highly selective micropipette sensor to precisely monitor Cu 2+ content has become crucial for the diagnosis, treatment, and prognosis of diseases. Herein, we propose a hydrogen-bonded organic framework (HOF-101)-functionalized micropipette sensor, which enables highly sensitive and real-time detection of copper ions based on an ionic current-voltage response. This micropipette sensor is capable of detecting Cu 2+ in human serum with a remarkably low limit of detection (LOD) of 0.11 μM, while offering a broad dynamic detection range of 10 –1 –10 4 μM. The adsorption mechanism was elucidated through density functional theory (DFT) calculations, revealing the coordination effect and electrostatic interactions between Cu 2+ and the -COOH groups of HOF-101. This study not only fulfills the requirements of simple and sensitive detection of Cu 2+ , but also provides a new method for HOFs to detect ions in vivo.
Escherichia coli O157:H7 ( E. coli O157:H7 ) emerges as a foodborne pathogen, emphasizing the imperative for creating precise detection tools. An ultrasensitive electrochemiluminescence/fluorescence (ECL/FL) dual-signal mode aptasensor was constructed for the detection of E. coli O157:H7 . Ultrafine silver nanoparticles were loaded on the surface of hydrogen-bonded organic skeleton materials by in-situ photoreduction method, and then combined with aptamers that can identify specific targets to prepare HOF-101@AgNPs@Apt, greatly simplifying the ECL/FL dual-signal mode probe fabrication process and improving sensing reliability. HOF-101@AgNPs@Apt had both strong ECL luminescence and fluorescence, resulting in a high detection sensitivity. The low limit of detection (LOD) for ECL and FL were 0.48 CFU mL −1 and 2.39 CFU mL −1 , respectively. Moreover, the proposed dual-signal mode aptasensor has been successfully applied to determine E. coli O157:H7 levels in tap water and milk with superior accuracy and high antiinterference capability, providing a promising method for food safety monitoring.
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