Baojie Zhu,Wenxia Zhang,Jing Zhao,Bilian Chen,Fei Liu,Shaoping Li
Grifola frondosapolysaccharides (GFPs) from different regions in China were characterized and compared using HPSEC-MALLS-RID and saccharide mapping based on HPAEC-PAD analysis for achieving and improving its quality control. The results showed that HPSEC chromatograms and molecular weight distributions of GFPs were similar. The average contents of each polysaccharide fraction (Peaks 1, 2, and 3) showed that Peak 3 was the main component and much higher than the other two polysaccharide fractions, which also contained protein. The result of saccharide mapping showed thatα-1,4-glycosidic,β-1,4-glycosidic and fewβ-1,3-glycosidic linkages were existed in GFPs. The similarity result showed that HPAEC-PAD fingerprints of the oligosaccharide fragments after hydrolysis by endoglycosidase were certainly different, especiallyα-amylase with a mean similar index of only 0.781 ± 0.207. The result of hierarchical cluster analysis (HCA) showed that different batches of GFPs from China can be divided into different clusters. Furthermore, immune-enhancing activity based on RAW 264.7 cells showed significant differences among different GFPs. Based on grey relational analysis (GRA), the fractions of Peak 3 were regarded as the major contributors to its immuno-enhancing activity in GFPs. Overall, the implications from these results were found to be stable, comprehensive, and valid for improving the quality control of GFPs.Keywords:Grifola frondosapolysaccharides;saccharide mapping;HPSEC-MALLS-RID;HPAEC-PAD;immunomodulatory
The polysaccharides from L. barbarum L. fruits (LBPs) are a kind of complex polysaccharides, which mainly contribute to the bioactivity of L. barbarum L. fruits and riches in acidic polysaccharides fractions. And the pharmacological research showed that the immunomodulatory effect of LBPs was closely related to α -1,4-D-galactosiduronic linkages. Therefore, as one of the main active polysaccharide fractions in LBPs, acidic polysaccharides fraction should also be used for the quality control of L. barbarum L. fruits. In this study, a novel quantitative evaluation strategy of polysaccharides from L. barbarum L. fruits based on simultaneous quantification of total polysaccharides and polysaccharides with active structure characteristics was proposed to improve their performance in the medicinal and functional food area more comprehensively and effectively, and beneficial to improve the quality control of L. barbarum L. fruits. The content of total polysaccharides in L. barbarum L. fruits was determined by HPSEC-RID method combined with universal dn / dc (0.15 mL·g −1 ). Additionally, HPAEC-PAD method combined with pectinase hydrolysis and m-hydroxybiphenyl colorimetric method were developed for the determination of acidic polysaccharide components related to active structural characteristics in L. barbarum fruits. The results revealed that the trends of total polysaccharides and acidic polysaccharides in L. barbarum L. fruits did not entirely coincide, further emphasizing the necessity of simultaneously quantifying both fractions for quality control. Furthermore, the m-hydroxybiphenyl method can be used as an alternative method to determine the content of acidic polysaccharides by HPAEC-PAD combined with pectinase hydrolysis.
危险品化学品经营许可证（带存储）