SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Dong Sun,Qi Chen,Bo Zhu,Hui Zhao,Shunshan Duan
Nonylphenol (NP) is a toxic xenobiotic compound, which is persistent in the aquatic environment and is extremely toxic to aquatic organisms. Although the exact molecular mechanisms of its toxic effect are well understood, the multigenerational reproduction and multigenerational - gene expression changes caused by NP still remain unclear. The following work investigated the effect of NP on four consecutive generations of zebrafish by examining their growth and several reproductive parameters, the degree of gonad damage, and the expression of related reproduction related genes. The results showed that high concentrations (20 and 200 μg·L −1 ) of NP could decrease growth and induce gonad damage in zebrafish. In addition, gnrh2 and gnrh3 genes were up-regulated, and fshβ and lhβ genes were downregulated in the hypothalamus in male zebrafish; while in female fish, the fshβ and lhβ were upregulated in P and F1 generations, and then down-regulated in the F2 generation. Meanwhile, the cyp19a1a gene was downregulated in the gonad of male fish, while the genes of fshr , lhr and esr showed a downward trend in females. Compared to P generation, F2 generation was more tolerant to higher NP concentrations (20 and 200 μg·L −1 ), as was also more sensitive to lower concentrations of NP (2 μg·L −1 ). Consequently, stress and damage caused by environmentally-relevant concentrations of aquatic pollutants in a vertebrate model were measured and predicted. Prevention and control measures can be actively and effectively proposed, which might be transversal to other exposed organisms, including humans. After several generations, typical transgenerational genetic phenomena might occur, which should be addressed by further studies.
研究领域:
环境科学
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毒理学
·
分子生物学
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DOI
PubMed
高影响因子
Advanced Therapeutics
·
2025
·
IF 2.6
Qi Tan,Yuwei Hu,Bo Wang,Songwei Tan,Yang Guan,Li Zhou,Qing Tang
Ulcerative colitis (UC) is a chronic inflammatory bowel disease with a need for more effective and less invasive treatment options. This study aimed to develop a targeted drug delivery system using hyperbranched polyglycidylglycerol-poly(lactic acid)-hydroxyacetic acid (HPG-PLGA)nanoparticles (NPs) loaded with baicalin (BN), a flavonoid with potent anti-inflammatory properties, to enhance therapeutic efficacy in UC. HPG-PLGA NPs are synthesized, characterized, and loaded with BN. The particle size, polydispersity index (PDI), encapsulation efficiency, and in vitro release profile of the NPs are evaluated. The in vivo biodistribution and therapeutic efficacy of the NPs are assessed in a dextran sulfate sodium-induced UC mouse model. The synthesized HPG-PLGA NPs demonstrated good stability and a controlled release of BN. In vivo studies showed significant accumulation of NPs in the inflamed colon, with a subsequent reduction in disease activity and inflammation markers. The treatment group exhibited lower levels of tumor necrosis factor-alpha, interleukin-1 beta, and interleukin-6 compared to the model group, indicating effective alleviation of inflammation. Furthermore, the NPs showed no significant toxicity to major organs. This study provides a promising approach for the development of targeted UC treatments, offering a potential clinical application by enhancing the bioavailability and specificity of BN to inflammatory sites.
研究领域:
药剂学
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纳米技术
·
胃肠病学
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炎症性肠病
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药物递送系统
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产品关联
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Bo-Cai Liu,Wei-Yan Xia,Shang-Qing Wang,Chang-Wei Xu,Zi-Li Liu
Spinel oxide NiCo 2 O 4 firstly has been explored to promote activity of Au for glycerol electrooxidation, which is supported on a three-dimensional hierarchical porous graphene-like carbon (3D HPG) material. Addition NiCo 2 O 4 into the Au/HPG catalyst can significantly improve the catalytic performance for glycerol oxidation. When NiCo 2 O 4 is added to the Au/HPG, onset potential is more 108 mV negatively than that on the Au/HPG. Peak current density on the Au/NiCo 2 O 4 (wt 6:1)/HPG is 5.1 times as high as that on the Au(20wt%)/HPG. The Au/NiCo 2 O 4 in this work shows a great potential as electrocatalyst for glycerol electrooxidation in alkaline medium.
研究领域:
电化学
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材料科学
·
催化反应
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2025
·
IF 7.3
Yufei Xie,Qiuyu Liu,Wenting Lin,Yuan Ren,Lingtian Xie
Various anabolic androgenic steroids (AAS) are frequently detected in aquatic environments, yet the potential ecotoxicological impacts of their increased load have not received adequate attention. This study investigates the effects of exposure to the androgen nandrolone on sex differentiation, gonadal development, and the expression of hormones, enzymes, and proteins related to the hypothalamic-pituitary-gonadal (HPG) axis in zebrafish. The results indicate that exposure to nandrolone at 60 days post-fertilization promotes the maturation of spermatogonia, inhibits the development of oocytes, and induces their apoptosis. Additionally, there were dose-dependent changes in male differentiation and hormone levels within the HPG axis: in the high-dose group (5 μg/L), males constituted 73 % of the population, and levels of E2 hormones in gonadal tissues decreased by 33 %, while T hormone levels increased by 55 %. Furthermore, the expression of genes essential for ovarian differentiation were downregulated, whereas the expression of testicular differentiation-related genes were upregulated. The molecular docking results show that the drug can form hydrogen bonds with key proteins, which have high affinity, and are more likely to cause conformational changes in the proteins, thereby altering their activity. KEGG analysis suggests that the MAPK signaling pathway is likely the target pathway through which nandrolone exposure induces reproductive toxicity.
研究领域:
生态毒理学
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分子生物学
·
内分泌学
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产品关联
DOI
PubMed
高影响因子
Toxins
·
2024
·Vol. 16
·期 3
·121
·
IF 3.9
Hira Sayed,Qiongqiong Zhang,Yu Tang,Yanan Wang,Yongpeng Guo,Jianyun Zhang,Cheng Ji,Qiugang Ma,Lihong Zhao
Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin found in many agricultural products and can cause reproductive disorders, mainly affecting spermatogenesis in male animals. Rutin (RUT) is a natural flavonoid compound recognized for its significant antioxidant, anti-inflammatory and estrogenic properties. The present study aimed to determine the protective role of RUT against ZEN-induced reproductive toxicity in male mice. Twenty-four adult Kunming male mice were divided into four groups: control, RUT (500 mg/kg RUT), ZEN (10 mg/kg ZEN), ZEN + RUT (500 mg/kg RUT + 10 mg/kg ZEN), with six replicates per treatment. The results indicated that RUT mitigated ZEN-induced disruption in spermatogenic cell arrangement, decreased spermatozoa count, and increased sperm mortality in the testes. RUT significantly restored ZEN-induced reduction in T, FSH, LH, and E2 serum levels. Moreover, RUT mitigated ZEN-induced apoptosis by increasing the mRNA expression level ofbcl-2, decreasing the mRNA expression level ofkiss1-r, and decreasing the protein expression level of caspase 8 in reproductive tissues. These findings indicate the protective role of RUT against ZEN-induced reproductive toxicity in male mice by regulating gonadotropin and testosterone secretions to maintain normal spermatogenesis via the HPG axis, which may provide a new application direction for RUT as a therapeutic agent to mitigate ZEN-induced reproductive toxicity.
研究领域:
毒理学
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生殖生物学
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分子生物学
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2025
·
IF 11.3
Yufan Nie,Simin Yu,Luyao Zhang,Zikang Wang,Rui Liu,Yuping Liu,Wentao Zhu,Zhiqiang Zhou,Jinling Diao
Declines in reptile populations due to climate warming and environmental pollution have been documented. Although recent ecotoxicological studies of reptiles have increased, little is known about how these two stressors interact to affect reptile reproductive processes. This study investigated the single and combined effects of atrazine and warming on reproduction in female lizards (Eremias argus) following 10 weeks of exposure to environmentally relevant concentrations of atrazine (0–10 mg·kg–1) at two temperature treatments (control or warming). Reproductive traits, clutch characteristics, and endpoints related to endocrine disruption (HPG axis gene expression) and energy metabolism (enzyme activity, hepatic metabolomics) were assessed. Atrazine inhibits female reproduction by disrupting HPG axis-related gene expression and energy metabolism, resulting in delayed spawning time and reduced fecundity. In contrast, warming promoted female reproduction and partly alleviated the inhibitory effects of atrazine, which is related to the upregulation of HPG axis-related gene expression and an additional energy metabolism compensatory response. Additionally, atrazine and/or warming altered the direction and intensity of the trade-off between egg number and size and affected maternal nutritional investment in eggs. These findings highlight the complex interplay of environmental stressors on lizard reproduction and add to a better understanding of reptile reproductive strategies and ecological consequences under environmental stress.
研究领域:
生态学
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毒理学
·
内分泌学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL SCIENCES
·
2018
·Vol. 34
·期 4
·433-438
·
IF 1.355
Li Hengye,Zhang Xuemeng,Zhang Lin,Cang Hui,Kong Fenying,Fan Dahe,Wang Wei
Surface-initiated anionic-ring-opening multibranching polymerization was employed to prepare a hyperbranched polyglycerol (HPG) functionalized silica stationary phase for hydrophilic interaction liquid chromatography (HILIC). The obtained stationary phase was characterized by Fourier-transform infrared spectrometry (FT-IR) and thermogravimetric analysis (TGA). The chromatographic properties of the prepared stationary phase were systematically investigated. The abundance and multitude distribution of hydroxyl groups in HPG endowed the stationary phase with improved hydrophilicity and enhanced separation performance compared with the stationary phase functionalized with monolayer of hydroxyl groups. The stationary phase showed excellent retention of various polar compounds, such as nucleosides, necleobases, phenols and sulfanilamides, indicating great potential in the separation of complex biosamples.
研究领域:
分析化学
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材料科学
·
色谱技术
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 74
·60596-60607
·
IF 3.84
Lulu Pan,Jianfeng Ban,Shaorong Lu,Guoxin Chen,Jin Yang,Qiyun Luo,Linyan Wu,Jinhong Yu
Perylene tetracarboxylic anhydride (PTCDA) was reacted with 6-aminocaproic acid to form the corresponding perylene bisimide (PBI). PBI was used as the foundation for oligomerisation of glycidol in a ring-opening reaction of glycidol leading to a hyper branched, water-soluble glycidol derivative of perylene (PBI-HPG). PBI-HPG was bound to the reduced graphene oxide via π–π stacking resulting in a compound termed PBI-HPG/RGO. The structure and morphology of PBI-HPG/RGO were investigated by infrared spectroscopy (FT-IR), wide angle X-ray diffractometry (WAXD), transmission electron microscopy (TEM), atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS). PBI-HPG/RGO was blended at different loadings in order to improve the thermal and mechanical properties of epoxy composites. The maximum Tg of the epoxy composites was about 20 °C and the decomposition temperature (Td) was 26 °C higher than that of neat epoxy. The incorporation of PBI-HPG/RGO yields a material with an impact strength of 39.6 kJ m−2 and a tensile strength at 0.7 wt%. It increased by 50.8% and 62.3%, respectively, compared to the neat epoxy.
研究领域:
材料科学
·
化学工程
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产品关联
DOI
高影响因子
QUIMICA NOVA
·
2023
·Vol. 46
·期 1
·20-28
·
IF 0.8
Zhou Meng,Sun Chunyu,Zhao Hong
Reducing non-specific adsorption of proteins on the surface of magnetic nanoparticles (MNPs) is becoming increasingly important. In this paper, we proposed a novel surface modification procedure by grafting hyperbranched polyglycerol (HPG) onto the surface of MNPs (Fe3O4@SiO2@MAA), in which lots of hydroxyl groups from HPG not only provide the hydrates sheath to prevent non-specific adsorption of proteins, but also react with succinic anhydride to generate carboxyl groups that serve as active sites to specifically bind proteins. The protein adsorption experiments showed that the non-specific adsorption (0.07 μg mg-1) was reduced to 4.58% of that before modification. It also showed that the antigen binding capacity was 9.7 times higher than the original when detecting cardiac troponin I (cTnI) in human plasma samples, which indicated that the final synthesized MNPs had great application prospects in bio-separation and bioanalysis.
研究领域:
材料科学
·
纳米技术
·
生物化学
·
生物医学工程
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产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2024
·
IF 8.4
Jianxiao Wang,Shuang Hao,Pengfei Qi,Wenliang Wang,Yunxia Hu
Polyamide reverse osmosis (RO) membranes with high separation performance and excellent chlorine-resistant properties are highly desirable. Many strategies have been developed to fabricate chlorine-resistant RO membranes but often at the expense of a trade-off between separation performance, chemical stability and chlorine-resistance. Herein, in this study, we grafted unique three-dimensional hyperbranched polyglycerol (hPG) onto the polyamide RO membrane to simultaneously improve the chlorine-resistance of the polyamide RO membranes without compromising separation performance. The effects of hPG grafting on the surface morphology , surface properties, as well as the perm-selectivity and the chlorine-resistance of the modified RO membrane were systematically investigated. Results show that the hPG grafted polyamide RO membranes reached a high water permeance of 3.27 ± 0.17 L m −2 h −1 bar −1 while maintaining an excellent NaCl rejection (99.30 ± 0.15%). Importantly, the hPG grafted PA RO membranes exhibit the impressive chlorine-resistance with the maximum exposure of 20,000 ppm·h where the NaCl rejection of the membrane was still 98.69 ± 0.39%, surpassing most of the reported chlorine-resistant polyamide RO membrane with the maximum exposure of 2000–16,000 ppm·h. Both simulation and XPS results suggest that the chlorine-resistant mechanism of the hPG grafted RO membrane is because of the reactivity inertness and steric hindrance of hPG, repelling chlorine away from the membrane surface and thus preventing the PA from chlorine attacking with the suppressed Orton rearrangement of the PA. Our findings demonstrate that the hPG grafted RO membrane possesses exceptional chlorine-resistance and high separation performance, which has a great potential for the practical applications.
研究领域:
材料科学
·
环境工程
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产品关联
DOI
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2025
·Vol. 32
·期 4
·1-13
·
IF 2.8
Lei Hengbo,Cao Xiang,Tang Yuguo
Monodisperse superparamagnetic poly (glycidyl methacrylate) microspheres (PGMA-HPG-COOH) with carboxyl group were synthesized and applied to treatment of cationic dyes wastewater. The composite adsorbent not only has good adsorption selectivity for cationic dyes (e.g., methylene blue (MB) and rhodamine 6G (R6G)), but also has convenient magnetic separation characteristics. The optimal pH for the adsorption of methylene blue was determined as pH 7, which was associated with the surface charge of adsorbent. Due to its ultra-high hydrophilicity, PGMA-HPG-COOH demonstrated a remarkably rapid adsorption rate for MB and R6G, and the adsorption saturation level of 95% can be achieved within just 10 min. The adsorption data showed nice correlation with pseudo-second-order kinetic model and Langmuir isotherm. The theoretical maximum adsorption capacity of PGMA-HPG-COOH for MB and R6G can reach 238.1 mg/g and 227.27 mg/g, respectively. Futhermore, PGMA-HPG-COOH can be effectively regenerated using a binary HCl-ethanol solution, maintaining the adsorption efficiency of 91.7% after five cycles of recycling. These findings substantiate the promising potential of the PGMA-HPG-COOH adsorbent for application in water treatment processes.
研究领域:
材料科学
·
环境工程
·
化学工程
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产品关联
DOI
高影响因子
GENERAL AND COMPARATIVE ENDOCRINOLOGY
·
2025
·
IF 1.7
Xuanhan Zhang,Chenpeng Zuo,Jiaqi Liu,Xiao Jing,Ziyi Zhao,Jing Yang,Zhijun Wang,Xin Qi
Gonadal development is closely linked to cortisol levels, a major stress response indicator, which is mediated by the glucocorticoid receptor (GR) in teleost fish. Stress activates the HPI axis (hypothalamic-pituitary-adrenocortical axis), triggering cortisol release, which subsequently affects the reproductive system. Therefore, GR is considered a mediator in elucidating the relationship between stress and gonadal development. In this study, we cloned and analyzed the gr1 and gr2 genes of the Japanese eel ( Anguilla japonica ). The phylogenetic tree of GR revealed that gr1 and gr2 exhibit a unique evolutionary distribution in teleost fish. Quantitative real-time PCR results indicated that gr1 and gr2 were primarily expressed in the ovary and brain. To investigate whether glucocorticoids mediate their inhibitory effects through GR, we performed in vivo DXMS injection experiments. The results indicated that DXMS stimulation significantly altered the expression of key genes in the HPG axis (hypothalamic-pituitary–gonadal axis) of the Japanese eel, particularly those related to ovarian development. Additionally, in vitro experiments with isolated brain, pituitary, and gonadal cells revealed that DXMS treatment significantly suppressed the expression of several reproduction-related genes, and the GR antagonist RU486 partially reversed this suppression. This study reveals the mechanisms through which glucocorticoids regulate ovarian development in the Japanese eel via GR and HPI-HPG axis interactions. These findings offer new insights into the role of stress in fish reproduction and provide a theoretical basis for optimizing artificial reproduction techniques in the Japanese eel.
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产品关联
DOI
PubMed
高影响因子
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