JOURNAL OF THE CHINESE CHEMICAL SOCIETY
·
2023
·
IF 1.8
Guohao Chen,Xiao Liu,Rui Zhang,Miaomiao Han,Shengxiang Ji
Bacterial colonization and biofilm formation on the surface of materials can lead to serious bacterial infections and are highly detrimental to our health. Here, we prepared a water-insoluble HPL-DOSS complex by compounding hyperbranched polylysine (HPL) with docusate sodium salt (DOSS) and coated HPL-DOSS onto the surfaces of the commercial polypropylene (PP) and thermoplastic polyurethane (TPU) plastics to afford antibacterial PP-HD and TPU-HD plastics. PP-HD and TPU-HD showed excellent antibacterial activities against both Escherichia coli and MRSA . In addition, the bacterial residues on the PP-HD and TPU-HD surfaces were significantly reduced compared with the pristine PP and TPU surfaces, indicating that the HPL-DOSS surface coating had good anti-adhesive property against bacteria and helped to reduce the formation of biofilms. Meanwhile, both PP-HD and TPU-HD exhibited excellent biocompatibility, indicating that the HPL-DOSS complex could be used as a safe antimicrobial coating material to reduce bacterial infections on plastic surfaces.
研究领域:
材料科学
·
生物医学工程
·
微生物学
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产品关联
DOI
高影响因子
Food Chemistry-X
·
2024
·
IF 6.5
Yue Tang,Chenguang Zhou,Zhiyang Yu,Meng Jiang,Yan Chen,Haiyan Wang,Zhen Yang
The aim of this study was to explore the formation of volatile lipid oxidation products by the lipoxygenase (LOX)-hydroperoxide lyase (HPL)-mediated pathway in oat, barley and soy bean. LOX activity was found only in barley and soy bean samples, but the lipase and HPL activity was detected in all samples. HPL showed particularly high activity with 13-hydroperoxides, while the activity was quite low when using 9-hydroperoxides, especially in the oat and barley. The optimum pH for HPL in different samples was similar, i.e., pH 6–7. In this condition, the volatile compounds formed dramatically with aldehydes and furans as the dominant products. Furthermore, a remarkable enzymatic degradation of lipids occurred during the preparation of food models with highly refined rapeseed oil (RO) and rapeseed oil fatty acid (ROFA) emulsions, where the ROFAs were more prone to oxidation than RO. This study shows the significance of lipid-degrading enzymes in plant-food flavour formation.
研究领域:
植物学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2022
·Vol. 41
·期 3
·345-355
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IF 3.815
Liu Hui,Liu Xiao,Cao Yuan-Qiao,Liu Ming,Han Miao-Miao,Ji Sheng-Xiang
Antimicrobial peptides (AMPs) have been considered as an alternative to small molecule antibiotics since they are difficult to develop antimicrobial resistance. Hyperbranched polylysine (HPL), an AMP mimics, has gained attention due to its broad-spectrum antibacterial activities, but it also suffers from high toxicity. Here we report a facile strategy to engineer the toxicity of HPL by copolymerizing lysine (K) with a hydrophobic amino acid, e.g. , alanine (A), tryptophan (W) or phenylalanine (F), to afford hyperbranched random copolymers. These copolymers have comparable antibacterial activities to HPL while their cytotoxicities and in vivo toxicities are lowered when the type and content of hydrophobic amino acid and the size of copolymers are optimized. The G. mellonella infection model demonstrates that the copolymers are effective against the S. aureus infection in vivo . The copolymers kill the bacteria through the disruption of cell membranes and the bacteria do not develop resistance to the copolymers.
研究领域:
材料科学
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生物医学工程
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微生物学
·
药学
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DOI
高影响因子
PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2018
·
IF 2.718
Qiong Wu,Xiaoya Tao,Xinzi Ai,Zisheng Luo,Linchun Mao,Tiejin Ying,Li Li
Fruit aroma development depends on ripening. Abscisic acid (ABA) has been reported to be involved in the regulation of tomato fruit ripening . In the present study, the effects of exogenous ABA on aromatic volatiles in tomato fruit during postharvest ripening were studied. The results showed that exogenous ABA accelerated color development and ethylene production as well as the accumulation of carotenoids , total phenolics and linoleic acid in tomato fruit during ripening. Moreover, exogenous ABA increased the accumulation of volatile compounds such as 1-peten-3-one (2.06-fold), β-damascenone (1.64-fold), benzaldehyde (3.29-fold) and benzyl cyanide (4.15-fold); induced the expression of key genes implicated in the biosynthesis pathways of aromatic volatiles, including TomloxC , HPL , ADH2 , LeCCD1B and SlBCAT1 (the values of the log 2 fold changes ranged from −3.02 to 2.97); and promoted the activities of lipoxygenase (LOX), hydroperoxide lyase (HPL) and alcohol dehydrogenase (ADH). In addition, the results of promoter analyses revealed that cis -acting elements involved in ABA responsiveness (ABREs) exist in 8 of the 12 key genes involved in volatile biosynthesis, suggesting that ABA potentially affects aromatic volatile emissions via the regulation of gene expression profiles.
研究领域:
植物生理学
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采后科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
PLANTA
·
2022
·Vol. 255
·期 4
·1-13
·
IF 4.54
Han Xueyuan,Wang Xiaoyu,Shen Chi,Mo Yiwei,Tian Rungang,Mao Linchun,Luo Zisheng,Yang Huanyi
Main conclusion
Exogenous ABA played a positive role in the accumulation and biosynthesis of aroma components of postharvest kiwifruit after low-temperature storage, especially the esters production during ripening.
Abstract Low-temperature storage (LTS) generally affects the aroma formation associated with the decrease in aroma quality in kiwifruit. In this work, abscisic acid (ABA) treatment after LTS increased the production of aroma components in postharvest kiwifruit and enhanced the related enzyme activity, especially alcohol acyltransferase (AAT), branched amino acid transaminase (BCAT) and hydroperoxide lyase (HPL). Corresponding to the enzyme activity, the gene expression of AchnAAT , AchnADH , AchnBCAT and AchnHPL was significantly up-regulated by ABA. The principal component analysis further illustrated the differences in aroma components between ABA and the control. The positive correlation of aroma accumulation with the expression levels of AchnPDC and AchnLOX and the enzyme activities of BCAT and pyruvate decarboxylase (PDC) was also revealed by correlation analysis. In addition, the promoter sequences of the key genes involved in aroma biosynthesis contained multiple cis-elements (ABRE and G-box) of ABA-responsive proteins. Combining the transcriptome sequencing data, the promoting role of ABA signaling in the regulation of aroma biosynthesis of postharvest kiwifruit after LTS was discussed. This study would provide a reference for improving aroma quality of postharvest kiwifruit after LTS, as well the molecular mechanism of kiwifruit aroma fading after LTS.
研究领域:
植物生理学
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采后生物学
·
分子生物学
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生物化学
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产品关联
DOI
PubMed
高影响因子
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