FOOD CHEMISTRY
·
2019
·
IF 5.399
Yun-Jiao Ma,An-Qi Bi,Xiao-Yuan Wang,Lei Qin,Ming Du,Liang Dong,Xian-Bing Xu
The dispersion solid-phase microextraction (DSPE) combined with dispersion liquid–liquid microextraction (DLLME) was developed as a rapid, simple and effective pretreatment method for the determination of flavor enhancers (maltol, ethyl maltol, vanillin, methyl vanillin, ethyl vanillin) of ready-to-eat seafood products (dried squid, baked squid, fried fish, steamed abalone). Under the optimized DSPE–DLLME method, the developed method exhibited low limits of quantitation (0.20–0.50 μg g −1 ) and excellent linearity (R 2 ≥ 0.995). The recoveries of flavor enhancers in the actual samples were 83.7%–114.9%. Compared with traditional pretreatment methods, the developed DSPE–DLLME method was rapid (17 min) and easy to determine the flavor enhancers by high performance liquid chromatography with photodiode array detector (HPLC-PDA). In the actual samples, creamy compounds such as vanillin, methyl vanillin and ethyl vanillin were hardly found. However, the flavor compounds produced by thermal reactions such as maltol (except for dried squid 3) and ethyl maltol were present in all samples.
研究领域:
食品科学
·
分析化学
·
色谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 68
·38715-38720
·
IF 2.936
Yin Qian,Xi Chen,Jin Qi,Xuming Liu
Protein tyrosine nitration plays a key role in many inflammatory and cardiovascular diseases and diabetes. Many natural products are used to treat these diseases through their ability to potentially interfere this reaction. Here, we describe a novel method to provide active fingerprinting of inhibition of tyrosine nitration by natural products based on post-column tyrosine nitration reaction analysis using high-performance liquid chromatography coupled to a photometric diode array. Results indicated that lotus leaf extracts exhibited obvious inhibitory activity against tyrosine nitration by peroxynitrite, and that chemical and active fingerprints were simultaneously established, with the active fingerprints indicating the active compounds of the lotus leaves. Additionally, flavonoids were screened as the principal active compounds involved in inhibiting tyrosine nitration in the lotus leaf extracts, with quercetin-3-O-glucuronide and quercetin-3-O-glucoside exhibiting the greatest contributions. Moreover, our results suggested that lotus leaves from three regions (Nanjing, Suzhou, and Hangzhou) exhibited the best inhibitory activity. These findings indicated the usefulness of this method for screening active compounds involved in inhibiting protein tyrosine nitration, and that similar strategies can likely be applied to evaluate the inhibitory activity against tyrosine nitration of other natural products.
研究领域:
分析化学
·
药理学
·
天然产物
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 18
·7518-7524
·
IF 1.938
Nengsheng Ye,Ting Gao,Jian Li
Molecularly imprinted polymers (MIPs) of dopamine (DA) were constructed on the surface of graphene oxide (GO), and attached inside the pores of hollow fibers (HFs) for the solid-phase microextraction (SPME) of DA. Scanning electron microscopy, thermogravimetric analysis and Raman spectroscopy indicated that GO–MIP composites were successfully synthesized and modified in the pores of HFs via ultrasonication. Compared with common HFs and HFs modified with non-imprinted polymers (GO–NIP/HF) using the same SPME procedures, the GO–MIP/HF composites showed the best efficiency for the extraction of DA. The selectivity of GO–MIP/HF was investigated based on the selectivity factor (F) using epinephrine and norepinephrine as the structural analogues of DA. The linear range of dopamine was 1.05 × 10−3 to 5.27 × 10−3 μmol mL−1, using this process with a detection limit of 2.64 × 10−4 μmol mL−1. The extraction procedure based on GO–MIP/HF was successfully used for the determination of DA in human serum and its hydrochloride injection, showing average recoveries of 83–96%. GO–MIP/HF was a good carrier for the selective adsorption of DA, and showed promise for the preconcentration of DA in real samples.
研究领域:
分析化学
·
药物分析
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
CHROMATOGRAPHIA
·
2019
·Vol. 83
·期 2
·205-217
·
IF 1.552
Li Li,Chen Naijiang,Gu Puzhong,Li Honglei,Li Mengqing,Zhang Ziyue,Chen Ruiling,Zhang Ming
Abstract Compound amino acid injection-18 AA (CAAI-18 AA) is widely applied in medical care as a part of total parenteral nutrition systems. Thus, suitable quality control of CAAI-18 AA is of crucial importance for safety of the patients. Tryptophan (TRP) is one of the main components in CAAI-18 AA and may be degraded to 5-hydroxy-tryptophan (5-HTP), 5-hydroxytryptamine (5-HT), kynurenine (KYN) and indole (IN) under certain conditions, such as oxidation environments, ultraviolet light radiation and nonsterile conditions. The existence of these harmful impurities seriously affects the quality of CAAI-18 AA. Here, a simple HPLC approach with a PDA detector was developed and validated for simultaneous determination of the concentrations of 5-HTP, 5-HT, KYN and IN impurities in CAAI-18 AA. Separation of these four impurities in CAAI-18 AA was achieved under a gradient condition using acetonitrile and 10 mM phosphate buffer (containing 10 mM sodium 1-octanesulfonate, pH adjusted to 1.8 with phosphoric acid). To detect the four analytes with high sensitivity, multi-wavelength detection at 257, 270 and 276 nm was carried out during the separation. The method was validated in accordance with ICH guidelines and the results were compared to that obtained by UHPLC–MS/MS, showing that the two approaches did not provide significantly different results. Our results reveal that the simple and less expensive HPLC method holds great potential for quantitation of the four impurities in CAAI-18 AA. Graphic Abstract
研究领域:
药学
·
分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2026
·
IF 4.6
Zhihao Yang,Tianle Shen,Jinhu Tian,Yanbin Wang,Xingqian Ye,Jianchang Jin,Dan Wu
The determination of phenolic compounds serves as a critical approach for food quality control and screening of bioactive phytochemicals. This study developed a high-performance liquid chromatography-photodiode array detection (HPLC-PDA)-based analytical technique for plant phenolics, enabling simultaneous accurate quantification of 37 representative phenolic compounds in samples. Method validation demonstrated excellent linearity, precision, and accuracy, with intra-day and inter-day precision below 3 %, and spiked recoveries ranging from 96.88 % to 103.75 %. The methodology was successfully applied to analyze 19 distinct plant specimens, confirming its exceptional versatility. This method can be used as an efficient assay for plant polyphenols in multiple fields including food analysis, phytochemical research, and bioactive component development.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2018
·
IF 2.831
Weilin Wu,Wenqi Qian,Haitian Hao,Yun kang,Yaqin Wang,Yuanrong Deng,Chengjie Ni,Jianming Huang,Weiyu Weng
Five main components of Azolla imbricata were isolated and identified as caffeoylquinic acid derivatives, four of which were isolated from this species for the first time. The five compounds exhibited strong antioxidant activities and were used as chemical markers for quantitative analysis. A chitosan-based matrix solid-phase dispersion (MSPD) extraction coupled with high-performance liquid chromatography (HPLC) was developed for the determination of the five analytes in A. imbricata. The optimal conditions for the chitosan-based MSPD extraction were as follows: low viscosity chitosan HL-1 as the dispersant, sample-to-dispersant mass ratio of 1:1, 10 mL of a methanol-sulfuric acid aqueous solution (0.2 M) (7:3, v/v) as the elution solvent, and a grinding time of 1 min. Compared with ultrasonic assisted extraction (UAE), the chitosan-based MSPD extraction exhibited higher extraction efficiency, consumed less solvent and time, and provided a cleaner extract. Compared with C 18 -based MSPD, the developed method was less expensive and more environmentally-friendly. The validated MSPD–HPLC method was efficient, reliable, and applicable to the quantification of caffeoylquinic acids in A. imbricata .
研究领域:
植物化学
·
分析化学
·
药用植物研究
✓ 已验证
DOI
高影响因子
Journal of Functional Foods
·
2020
·
IF 3.701
Xiao Guo,Kaikai Li,Ailing Guo,Erhu Li
In this study, an in vivo animal experiment was performed, to investigate the intestinal absorption and distribution of naringin, hesperidin, and their metabolites by using UPLC-ESI-MS/MS and HPLC-PDA analysis. The bioavailability of naringin or hesperidin in duodenum, jejunum, ileum, cecum, and colon was calculated. A total of 7 and 13 metabolites were found and tentatively identified in intestinal tissue after orally administered naringin and hesperidin in mice, respectively. Sulfates of naringin could be absorbed by ileum and cecum, de-glycosylation metabolites could be absorbed by duodenum, and phenolic acids could be absorbed by the cecum and colon. Sulfates of hesperidin could be absorbed by duodenum and ileum, de-glycosylation metabolites could be absorbed by cecum, and phenolic acids could be absorbed by ileum, cecum and colon. Jejunum did not absorb metabolites of naringin or hesperidin. Results could be beneficial for understanding the intestinal bioactivities of naringin and hesperidin.
研究领域:
药理学
·
生物化学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Zhaoyuan He,Xinrui Shi,Yawen Guo,Fanxun Guan,Pengfei Gao,Yayun Tang,Shuyu Liu,Yali Zhu,Kaizhou Xie,Hailan Chen
Antimicrobial drugs are frequently used in a combination or shuttle way to cope with coinfection of bacteria or parasites and prevent drug resistance, thus the accurate quantification of multiple drug residues in animal-derived foods is crucial to ensure food safety. Here, a simple and efficient high-performance liquid chromatography-photodiode array (HPLC-PDA) method was established for the simultaneous quantitative screening of six common residues of antiparasitic drugs, including abamectin (ABM), ivermectin (IVM), albendazole (ABZ) and the three metabolites of ABZ in beef and chicken. The LODs and LOQs for six target compounds in beef and chicken are determined to be 3.2 to 12.5 µg/kg and 9.0 to 30.0 µg/kg, respectively. The calibration curves show good linearity (R 2 ≥ 0.9990) between the peak area and concentration. The recoveries from the fortified blank samples are all above 85.10%. Finally, the applicability of the HPLC-PDA method is successfully demonstrated by the real sample analysis.
研究领域:
分析化学
·
食品科学
·
兽医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2021
·
IF 3.205
Dandan Wang,Rui Liu,Jinxiang Zeng,Chunhu Li,Wei Xiang,Guoyue Zhong,Zhining Xia
An in vitro intestinal absorption model combined with high-performance liquid chromatography-photo diode array-tandem mass spectrometry (HPLC-PDA-MS) was used for preliminary screening of potential active ingredients from complex multi-component traditional Chinese medicine (TCM) system. Oral administration is one of the main administration methods for TCMs. Only the ingredients that could be absorbed have the opportunity to play a role. Thus, these were defined as potential active ingredients. Studying of intestinal absorption can provide a theoretical basis for the mechanism of TCMs. The Caco-2 cell model, the everted rat gut sac model, and the Ussing chamber model were established for TCMs. The degree of anastomosis between the in vitro intestinal model and the actual intestinal absorption of TCMs were evaluated by the gavage method in rats. The Ussing chamber model was best fit for oral experiments in rats and was selected as the research means to preliminarily screen potential active ingredients from eight TCMs, including Salvia miltiorrhiza Bunge, Astragalus propinquus Schischkin, Plantago asiatica L , Fallopia multiflora (Thunb.) Harald, Epimedium brevicornu Maxim, Moutan Cortex , Citrus reticulata Blanco , and Panax notoginseng (Burkill) F. H. Chen ex C. H. Chow. A total of 44 components were absorbed and screened as the potential active ingredients from the 80 components identified in eight TCMs by HPLC-PDA-MS.
研究领域:
药理学
·
分析化学
·
传统医学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Zhejiang University-SCIENCE B
·
2013
·Vol. 14
·期 7
·604-614
·
IF 1.108
Wang Shu-fang,Leng Jing,Xu Yi-min,Feng Mei-ling
Xiongdankaiming tablet (XDKMT), a well-known compound in traditional Chinese medicine, is widely used for the treatment of acute iridocyclitis and primary open-angle glaucoma. In this paper, accurate and reliable methods were developed for the identification of 20 constituents using high-performance liquid chromatography with photodiode array and electron spray ionization-mass spectrometry (HPLC-PDA/ESI-MS n ), and determination of nine of the constituents (chlorogenic acid, gentiopicroside, isochlorogenic acid B, diosmetin-7- O -β- d -glucopyranoside, apigenin, diosmetin, tauroursodeoxycholic acid, acacetin, and taurochenodeoxycholic acid) was developed using HPLC with ultraviolet absorption detector and evaporative light scattering detector (HPLC-UV/ELSD) for the first time. The best results were obtained on a Zorbax SB-C18 column with gradient elution using water (0.1% formic acid) (A) and methanol (0.1% formic acid) (B) at a flow rate of 0.7 ml/min. Tauroursodeoxycholic acid and taurochenodeoxycholic acid, owing to their low UV absorption, were detected by ELSD. The other seven compounds were analyzed by HPLC-UV with variable wavelengths. The calibration curves of all nine constituents showed good linear regression ( R
2 >0.9996) within the linearity ranges. The limits of detection and quantification were in the ranges of 0.0460–9.90 μg/ml and 0.115–24.8 μg/ml, respectively. The accuracy, in terms of recovery, varied from 95.3% to 104.9% with relative standard deviations (RSDs) less than 4.4%. Precision (with the intra- and inter-day variations less than 4.4%) was also suitable for its intended use. The developed method was successfully applied for the analysis of major components in XDKMT, which provides an appropriate method for the quality control of XDKMT.
研究领域:
中药学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 6
·932
·
IF 4.7
Yan Gou,Donglin Gu,Jing Fan,Jianbo Yang,Ke Zan,Jingjing Liu,Hongyu Jin,Ying Wang,Feng Wei,Shuangcheng Ma
Polygonum multiflorumThunb. (PM) and derived products are broadly utilized in Chinese traditional medicine. According to our previous research, PM mostly contains polysaccharides, which display a wide range of biological activities. Two water-soluble polysaccharides (PMPs-1 and PMPs-2) were obtained from PM by DEAE-Cellulose and Sephadex G-100 column chromatography. Colorimetry, HPGPC-MALLS-RID, HPLC-PDA, methylation, FT-IR, NMR, and SEM were used to characterize these polysaccharides. PMPs-1 and PMPs-2 had average molecular weights of 255.5 and 55.7 kDa, respectively. PMPs-1 consisted of Man, Glc, Gal, and Ara at 0.9:78.6:1.0:1.6 and was a glucan with → 4)-Glcp-(1 → as a backbone. Meanwhile, PMPs-2, an acidic polysaccharide, comprised Rha, GalA, Glc, Gal, and Ara at 3.2:20.3:2.7:1.0:8.3. PMPs-1 and PMPs-2 significantly improved the proliferation of RAW 264.7 cells and induced NO, TNF-α, and IL-6 release. This study reveals that these two polysaccharides can be explored as novel immunomodulators and provide a basis for further development of PM in food and pharmaceutical industries.
研究领域:
药理学
·
免疫学
·
生物化学
·
中医
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2024
·
IF 1.6
Jingjing Gu,Chunyang Chen,Tingting Liu,Yanyan Li,Xuedong Wang
Here is reported effervescent reaction-enhanced switchable hydrophilic-hydrophobic solvent-based salting-out microextraction (ERSSM) with high-performance liquid chromatography with photodiode array detection (HPLC-PDA) for the preconcentration, extraction, and trace-level determination of polybrominated diphenyl ethers (PBDEs) in milk and water. The important variables were rigorously optimized to be 150 µL of hexanoic acid as the extraction solvent, 400 µL of Na2CO3 as the alkaline source, 350 µL of H2SO4 solution as the acid source, and NaCl as the salting-out agent. The optimized conditions provided a linear range from 0.2 to 50 µg L−1 with limits of detection and quantitation from 0.026 to 0.085 µg L−1 and 0.087 to 0.28 µg L−1 for six PBDEs. The intra-day and inter-day precision values were from 2.48% to 6.75%, and the fortified recoveries for PBDEs were between 78.06% and 103.07% in water and milk samples. The developed procedure avoids the use of a traditional organic disperser and mechanical mixing allowing the determination of PBDEs outdoors by synchronous extraction method and high efficiency for the analytes. These advantages suggest a high potential for the routine monitoring of PBDEs in liquid food and environmental samples.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）