Food Chemistry-X
·
2024
·
IF 6.5
Qian Han,Min Yang,Zexin Zhang,Xinwen Bai,Xiuying Liu,Zhenhua Qin,Wei Zhang,Pingping Wang,Lijie Zhu,Zaixi Shu,Xuepeng Li
In this study, amine vapor-sensitive films with ratiometric fluorescence attributes were developed. The pH-sensitive fluorescein 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS) and its tetraphenylethylene derivative (TPB) were selected as ratiometric indicators and incorporated into a polyvinyl alcohol (PVA) matrix to produce HPTS/TPB-PVA films. The films responded well to amine vapors, and the interference of aromatic vapors did not substantially affect the fluorescence signals of the films. Under UV light at a wavelength of 365 nm, the fluorescence of the films changed from dark pink to light pink and finally to yellow when the freshness of the fish was visually checked during storage. In addition, the color difference values of the films showed a positive correlation with the total volatile basic nitrogen (TVB-N), ranging from 12.7 to 24.8 mg/100 g at 25 °C and 8.4 to 25.6 mg/100 g at 4 °C, respectively. This indicates that fluorescent films have good potential for quantifying fish freshness in the near future when connected to an automatic data processing system based on color differences.
研究领域:
食品科学
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生物传感
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材料科学
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产品关联
DOI
PubMed
高影响因子
LUMINESCENCE
·
2019
·Vol. 34
·期 6
·558-562
·
IF 1.691
Mingwei Guo,Weijie Li,Yanan Sun,Huimin Xu,Yiling Fan,Liyan Ma,Kunlun Huang,Zhiyi Yao
In this study, a rapid method for the detection of berberine hydrochloride (BRH) was developed based on a water-soluble pyrenyl probe, 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS). This method features low cost, good selectivity, high sensitivity and a fast response. The sensing mechanism of this probe is attributed to the formation of a complex between HPTS and BRH induced by electrostatic interaction and π–π stacking. To the best of our knowledge, this is the first fluorescent sensor for BRH based on organic materials that has low cost and a visual response. The detection limit of this method was as low as 1.24 μM and the linear response range is 2–50 μM. This method also allowed rapid detection of BRH real samples.
研究领域:
分析化学
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药物分析
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有机化学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2020
·
IF 3.594
Jincan He,Yuyuan Su,Zijun Sun,Runkun Zhang,Fuhai Wu,Yan Bai
In this paper, a fluorometric method based on chitosan-mediated “turn-on” strategy was proposed for rapid detection of perfluorooctane sulfonate (PFOS). The method can be performed by two steps of simple physical mix. The whole procedure takes no more than 25 min. In pH 5.50 Britton-Robinson buffer solution, a green fluorescent dye (trisodium-8-hydroxypyrene-1,3,6-trisulfonate, HPTS) reacted with protonated chitosan to form complex by electrostatic attraction, leading in the strong quenching of HPTS. In the presence of PFOS, the fluorescence of HPTS recovered resulting from the electrostatic and hydrophobic interactions between PFOS and chitosan. The spectral characteristics, the reaction mechanisms of fluorescence quenching and fluorescence recovery, and the analytical conditions were investigated. Under the optimum conditions, a fluorometric method based on the chitosan-mediated “turn-on” strategy was developed for detection of PFOS. The value of fluorescence restoration (Δ F ) was proportional to the concentration of PFOS in range of 0.0050–2.0 μmol/L with correlation coefficient of 0.9949. The limit of detection (LOD) was calculated to be 1.0 nmol/L. The method was applied to the determination of PFOS in environment water with recoveries of 90.8–102% and RSD of 2.8–5.9%. The fluorometric method based on chitosan-mediated “turn-on” strategy is simple, rapid, sensitive and economical, showing potentiality for detection of PFOS in environmental water samples.
研究领域:
分析化学
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环境科学
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产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 29
·期 14
·20593-20602
·
IF 4.223
Kong Shuai,Shen Chong,Meng Qin
The extensive application of chemically synthesized anionic surfactants would cause serious pollution of water and increase health risk to humans. However, the adverse impact of anionic surfactant on human cells has never been systematically demonstrated. In this paper, a series of fluorescent anionic surfactants containing a varying length of alkyl chain from C8 to C18 and a fixed hydrophilic head of 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS) were synthesized and utilized for visualizing the interaction between surfactants and cells. The obtained molecules exhibited blue fluorescence presenting a decreasing fluorescent intensity with the increasing length of alkyl chain from C8 to C18 while showed the same sequence of HPTS-C16>HPTS-C18>HPTS-C12>HPTS-C8 on either surface activity, cellular adsorption, or cytotoxicity. In opposite, HPTS which contained no hydrophobic chain and thus exhibited no surface activity showed no cellular adsorption and cytotoxicity. It seems that the ligand of the appropriate chain length (C16) onto the hydrophilic HPTS molecules could cause the largest surface activity, the most distinguished cellular adsorption as well as the most adverse cytotoxicity. As reflected by the dynamic fluorescent visualization, the surfactant molecules of HPTS-C16 initially bound with cell membrane and entered into the intracellular lumen before finally localized at the endoplasmic reticulum (ER) and damaged it into a swollen structure. It is most likely that the structure of hydrophobic chain could determine the surface activities of surfactants and hence affect their cellular uptake and cytotoxicity. This study could help us to understand the adverse impact of anionic surfactant on human cells and its correlation with the surface activities or, in another word, the hydrophobic chain length.
研究领域:
细胞生物学
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毒理学
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环境科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Yexin Shen,Ran Hong,Xin He,Cong Wang,Xiuyuan Wang,Shantao Li,Xiandong Zhu,Daxiang Gui
Convenient, rapid, and accurate detection of nitroaromatic organic toxins and harmful substances is of great significance in research. In the present study, two-dimensional layered rare-earth hydroxides (LYH) were used as ion-exchange matrix materials, and the anionic fluorescent dye molecules (HPTS) were successfully introduced into the LYH structures in situ via a simple and effective “plug-and-play” strategy, which gave the compounds ultra-sensitive fluorescence sensing detection of nitrobenzene, p-nitrotoluene and p-nitrophenol (Fluorescence response time < 1 sec , and the LOD for nitrobenzene, p-nitrophenol and p-nitrotoluene reached an impressive 349 ppb, 22 ppb and 98 ppb, respectively). Combined with theoretical calculations, we elucidated in detail the fluorescence quenching response mechanism of the LYH-HPTS towards nitroaromatic. Additionally, we also constructed fluorescent paper sensor, which effectively transformed the LYH-HPTS from theoretical detection to device application. The LYH-HPTS material is not only simple to synthesize, cost-effective and stable, but also has the features of fast response, excellent sensitivity and selectivity, and good reproducibility, which provides a new approach for the rapid and accurate detection of nitroaromatic.
研究领域:
分析化学
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材料科学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）