Silicon-based optical biosensors have garnered significant interest since their inception. They are, however, susceptible to temperature fluctuations in a controlled environment. In order to address this issue, we provide a solution including the utilization of a silicon-on-insulator (SOI) cascaded micro-ring resonators (CMRR) biosensor that is designed to be insensitive to temperature changes. It consists of two ring resonators exposed to the reference solution and analyte, respectively, their radius and waveguide width are designed to meet the temperature - insensitivity criterion of the CMRR sensor. The results of experiments indicate that by real-time monitoring of the envelope peak drift in the transmission spectrum of the sensor, the bulk refractive index (RI) sensitivity is 2940.71 nm/RIU, the limit of detection (LOD) is 3.1×10 −5 RIU, and the temperature sensitivity is less than 40 pm/℃. Furthermore, the sensor was applied for HSA detection, with a calculated LOD of 18.09 ng/mL. The effect of temperature on HSA detection is much smaller than that of a CMRR sensor without athermal design. The sensor demonstrates high sensitivity and temperature insensitivity, and the absence of negative thermo-optic coefficient materials or supplementary temperature control mechanisms simplifies its practical applications. It can be made by a large array to detect different type protein molecules for high throughput multiparameter analysis.
Xue Liu,Shuang Qi,Jingyao Mao,Yuteng Zhang,Bin Dong,Bo Song,Lingling Ji
In this study, an amphiphilic organic fluorescent molecule (denoted by BCPB) was employed as a ratiometric fluorescent probe for the detection of human serum albumin (HSA). In an aqueous solution, the micellar assemblies of BCPB showed a strong excimer emission at 660 nm. The presence of HSA can induce the disassembly of BCPB aggregates and reassemble with HSA to form BCPB-HSA complexes, resulting in a ratio change in the fluorescence emission of BCPB at 540 nm (monomer) and 660 nm (aggregate). Accordingly, the fluorescence color also undergoes a visually recognizable change from red to green. As a ratiometric fluorescence probe of HSA, BCPB has a fast response (<5 s), low limit of detection (8.3 nmol L −1 , S / N = 3), good selectivity, and strong anti-interference ability. Finally, BCPB was loaded into the hydrogel to prepare a portable hydrogel kit, which successfully realized the in situ visual detection of HSA in an aqueous solution and human serum.
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