Molecular Medicine Reports
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2015
·Vol. 12
·期 5
·6656-6662
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IF 1.554
Chengfei Zheng,Hui Niu,Ming Li,Hongkun Zhang,Zhenggang Yang,Lu Tian,Ziheng Wu,Donglin Li,Xudong Chen
Aortic aneurysm is a cardiovascular condition with a serious risk of mortality and the dismal prognosis of any type of major cardiovascular disease. The present study found that tissues from aortic aneurysm patients and hypoxic aortic smooth muscle cells showed aberrant high expression of the cyclic RNA hsa‑circ‑000595, as demonstrated by polymerase chain reaction array screening. Knockdown of hsa‑circ‑000595 resulted in a decreased apoptotic rate of human aortic smooth muscle cells. Furthermore, it was determined that miR‑19a is a target of hsa‑circ‑000595. The results of the present study laid an epigenetic foundation for exploring the underlying mechanisms of the development of aortic aneurysm.
研究领域:
心血管疾病
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分子生物学
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表观遗传学
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DOI
PubMed
高影响因子
iScience
·
2023
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IF 5.8
Shuai Chen,Peng Song,Yu Wang,Zeng Wang,Jiaming Xue,Yicheng Jiang,Yan Zhou,Jie Zhao,Liming Tang
Circular RNA (circRNA) is a special category of non-coding RNA that has garnered increasing attention in the exploration of lipid metabolism. However, the functional regulation mechanisms of circRNAs in obesity diseases remain unclear. By whole transcriptome sequencing, a total of 164 circular RNAs were found to exhibit differential expression between lean and obese individuals. RT-qPCR was used to detect significant expression of circMAPK9 in obese individuals, and it was closely related to BMI. Western blot, triglyceride detection, and Oil Red O staining were employed to investigate the role of circMAPK9/hsa-miR-1322/FTO in adipogenesis. In adipocytes, the connection between hsa-miR-1322 and circMAPK9 was verified using fluorescence in situ hybridization, luciferase reporter assay, and RNA immunoprecipitation. It was found that circMAPK9 competed for binding hsa-miR-1322 in the cytoplasm, weakening the inhibitory effect on FTO and promoting adipogenesis. Our study revealed the regulatory mechanism and important role of circMAPK9 in the process of adipogenesis.
研究领域:
分子生物学
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基因组学
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代谢
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DOI
PubMed
高影响因子
TALANTA
·
2017
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IF 4.162
Shanshan Huang,Fangfang Li,Caiyun Liao,Baozhan Zheng,Juan Du,Dan Xiao
A simple fluorescent probe HBI-GR based on the combination of the fluorophore (p-HBI) in green fluorescent protein (GFP) and Guanine riboside (GR) for HSA was successfully synthesized. HBI-GR showed an obvious fluorescence enhancement toward HSA without interference from other proteins, amino acids, anions and commonly existing metal ions . HBI-GR exhibited high sensitivity towards HSA with a good linear relationship between the fluorescence intensity of HBI-GR and HSA concentration from 0 to 0.06 mg mL −1 . The limit of detection, based on a signal-to-noise ratio of 3, was 15.09 ng mL −1 , which was much lower than that of most other reported probes. HBI-GR was almost non-fluorescent because of the bond twisting in the exited state of chromophore HBI. After binding to the hydrophobic pocket of HSA, it showed an obvious fluorescence enhancement due to the rigidifying of the flexible chromophore HBI by the hydrophobic environment. The resulting HBI-GR/HSA system also showed a satisfactory sensing ability toward trypsin through decreased fluorescence intensity with the detection limit of 0.0282 ng mL −1 . The fluorescence decreasing process was occurred as the lysine and arginine amino acids residues of HSA were cleaved by trypsin, which led to further exposure of HBI-GR to the PBS buffer phase and a concomitant decrease of the HBI-GR fluorescence intensity. Moreover, the probe HBI-GR was successfully used to detect HSA in healthy human urine and human blood serum samples. The practical application of the HBI-GR/HSA system for trypsin detection in healthy human urine also achieved satisfactory result.
研究领域:
生物化学
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分析化学
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DOI
PubMed
高影响因子
Molecular Genetics & Genomic Medicine
·
2020
·Vol. 8
·期 5
·e1195
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IF 1.995
Fei Cui,Zhe-xue Hao,Jin Li,Ya-lei Zhang,Xu-kai Li,Jian-xing He
Background This study is aimed to unravel the genetic factors associated with microRNA (miRNA) expression in regulating sex-determining region Y-box 2 ( SOX2 )-mediated cisplatin resistance in small-cell lung cancer (SCLC). Methods The relevance of SOX2 expression in SCLC was analyzed in a panel of SCLC cells by quantitative real-time PCR (qPCR) and western blot (WB). We selected DMS114 cell line, in which SOX2 was amplified via lentiviral vector-mediated transfection of the SOX2 genes and tested for the half-maximal inhibitory concentration (IC 50 ) by MTS assay. High-throughput sequencing and screening of differentially expressed miRNAs between SOX2 -overexpressing and normal control cells were performed. Finally, miRanda software was used to verify the miRNAs bound with SOX2 and qPCR was used to identify the expression of miRNAs which were binding with SOX2 . Results Cisplatin-resistant SOX2 -overexpressing DMS114 cell lines were successfully developed, showing a statistically significant increase in SOX2 expression by qPCR and WB. Our results showed a typically higher IC 50 value in SOX2 -overexpressing cells compared with the negative controls. The high-throughput sequencing analysis revealed that 68 miRNAs were upregulated and 24 miRNAs were downregulated in the SOX2 -overexpressing cells. The 24 downregulated miRNAs were further verified. Of them, a cancer-related miRNA, hsa-miR-340-5p, showed a higher binding affinity with SOX2 in network regulation mapping, which was also found to be markedly downregulated under qPCR analysis. Conclusion We demonstrated that downregulated expression of hsa-miR-340-5p may affect cisplatin resistance by mediating SOX2 expression in SCLC cells, which may provide a potential target for the therapy of chemoresistant SCLCs.
研究领域:
肿瘤学
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分子生物学
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医学遗传学
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DOI
PubMed
高影响因子
REACTIVE & FUNCTIONAL POLYMERS
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2024
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IF 4.5
Huan Liu,Xiao-jie Zhang,Yu-xin Ding,Shao-hui Xiong,Zi-en Fu
12-hydroxystearic acid (HSA) was introduced into the thermoset benzoxazine, we expected that the stable ordered structures could be introduced into the thermoset three-dimensional network through the self-assembly of HSA and the polymerization-induced phase separation of benzoxazine monomers. To better understand the structural effects attributed to the introduction of HSA, some model compounds possessing the chemical structure-like HSA were also involved into the study. HSA and benzoxazine (Ba-bz) mixed with different weight ratios, an isotropic solution could be formed after the mixture melted, indicating that HSA molecules in Ba-bz monomers could assemble into ordered structures like in other organic solvents. The study found that the HSA molecules did not aggregate together in the form of individual molecule which completely distinguished from the model compounds. A conjunction of observation of macroscopic structure picture and characterization of microstructure of the samples, the results showed that HSA molecules spontaneously assembled into the fibrillar networks (SAFINs) in Ba-bz. Nanometric spherical micelles consist of HSA could be formed in the molten isotropic solution of HSA and Ba-bz and aggregate into tubes upon cooling. Due to the high concentration of HSA, the nanometric spherical micelles were easier to self-assembly into the large scale fibrillar tubes. During the polymerization, the fibrillar tubes could be stacked further under the action of the polymerization-induced and the rapid formation of the cross-linking network of polybenzoxazine stabilized the tubes. The tubular structure of HSA aggregation was filled with the polybenzoxazine, contributing to the formation of the interpenetrating structure.
研究领域:
材料科学
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生物化学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2024
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IF 8
Zhaoyu Wang,Mingyu Li,Xin Chen,Kaihao Zhao,Jiamei Gu,Jian-Jun He,Qiu-Shun Li,Wen-Fei Dong
Silicon-based optical biosensors have garnered significant interest since their inception. They are, however, susceptible to temperature fluctuations in a controlled environment. In order to address this issue, we provide a solution including the utilization of a silicon-on-insulator (SOI) cascaded micro-ring resonators (CMRR) biosensor that is designed to be insensitive to temperature changes. It consists of two ring resonators exposed to the reference solution and analyte, respectively, their radius and waveguide width are designed to meet the temperature - insensitivity criterion of the CMRR sensor. The results of experiments indicate that by real-time monitoring of the envelope peak drift in the transmission spectrum of the sensor, the bulk refractive index (RI) sensitivity is 2940.71 nm/RIU, the limit of detection (LOD) is 3.1×10 −5 RIU, and the temperature sensitivity is less than 40 pm/℃. Furthermore, the sensor was applied for HSA detection, with a calculated LOD of 18.09 ng/mL. The effect of temperature on HSA detection is much smaller than that of a CMRR sensor without athermal design. The sensor demonstrates high sensitivity and temperature insensitivity, and the absence of negative thermo-optic coefficient materials or supplementary temperature control mechanisms simplifies its practical applications. It can be made by a large array to detect different type protein molecules for high throughput multiparameter analysis.
研究领域:
生物医学工程
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光学传感
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分子检测
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DOI
高影响因子
JOURNAL OF MOLECULAR NEUROSCIENCE
·
2025
·Vol. 76
·期 1
·4
·
IF 2.7
Wu Zhixuan,Wang Dianyi,Yao Jing,Zhang Yue,Liu Jiayu
Background Migraine is a complex neurological disorder. While acupuncture is effective in alleviating migraines, its molecular mechanisms remain unclear. This study aims to explore these mechanisms. Methods We used bioinformatics to identify differentially expressed microRNAs (miRNAs) in migraine patients after acupuncture. Subsequently, luciferase reporter assays were utilized to verify the interaction between miRNAs and their regulated target gene mRNAs. RT-qPCR and Western blot analyzed key miRNAs’ regulatory effects on target genes. We also employed Transwell migration assays and a nitroglycerin (NTG)-induced migraine mouse model to systematically evaluate acupuncture’s efficacy and mechanism. Results Bioinformatics analysis revealed a significant upregulation of Hsa-miR-550a-3-5p in the peripheral blood of migraine patients after acupuncture treatment. Hsa-miR-550a-3-5p could directly inhibit the mRNA and protein expression of platelet and endothelial cell adhesion molecule 1 (PECAM1), reducing nitroglycerin-induced human brain microvascular endothelial cells (HBMECs). Following the downregulation of PECAM1 by hsa-miR-550a-3-5p, the transendothelial migration capacity of monocytes-macrophages was significantly reduced, and reducing the secretion of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6. Furthermore, it alleviates inflammation by regulating M2 macrophage polarization. In NTG-induced mice, acupuncture relieved migraine-like behaviors, an effect abolished by a miR-550a-3-5p inhibitor. Conclusion This study is the first to reveal that after acupuncture treatment, hsa-miR-550a-3-5p can be upregulated to target and inhibit PECAM1, reducing the transendothelial migration of inflammatory cells and the release of inflammatory cytokines, thereby alleviating migraine symptoms. This discovery provides scientific evidence for the efficacy of acupuncture and offers a new target for developing miRNA-based precision treatment strategies.
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DOI
PubMed
高影响因子
Clinical & Translational Oncology
·
2021
·Vol. 23
·期 9
·1866-1873
·
IF 3.405
Bai Z. M.,Li X. F.,Yang Y.,Yang Y. F.,Lv D. R.,Tang L. L.
Purpose The aim of the present study was to elucidate the functional role of hsa-miR-328-3p/STAT3 pathway in the effects of propofol on gastric cancer proliferation. Methods Bioinformatics was used to analyze the molecular expression differences of hsa-miR-328-3p/STAT3 axis in stomach adenocarcinoma ( n = 435) and normal samples ( n = 41) from TCGA database. The expression of the above molecules in gastric cancer cells SGC-7901 and normal gastric mucosal cells GES-1 was verified via qPCR. The dual-luciferase assay was carried out to confirm the interaction between hsa-miR-328-3p and STAT3. Subsequently, the cell proliferation and the expression of the above molecules in SGC-7901 and GES-1 cells were evaluated after 10 μM propofol treatment. Finally, we analyzed whether propofol still inhibited the proliferation of gastric cancer by suppressing STAT3 pathway after hsa-miR-328-3p down-regulation. Results Compared with normal samples, the expression of hsa-miR-328-3p was significantly down-regulated in stomach adenocarcinoma samples, while the expression of STAT3 and downstream target genes (MMP2, CCND1 and COX2) was up-regulated. The results were consistent with those in GES-1 and SGC-7901 cell lines. Meanwhile, we found that hsa-miR-328-3p can bind to the 3′-UTR of the potential target gene STAT3. Furthermore, propofol significantly inhibited the proliferation of gastric cancer cell line SGC-7901, where hsa-miR-328-3p was up-regulated and the expression of STAT3 and downstream proliferation-related target genes were down-regulated. However, the growth inhibition of propofol on SGC-7901 cell was significantly reversed after the inhibition of hsa-miR-328-3p. Conclusions To sum up, propofol suppressed the STAT3 pathway via up-regulating hsa-miR-328-3p to inhibit gastric cancer proliferation.
研究领域:
肿瘤学
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分子生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
XENOBIOTICA
·
2025
·
IF 1.2
Junling Zhu,Rilian Lai,Qin Zheng,Shuying Huang,Shenghua Hu,Zhangfei Xu,Huixia Sun
1. To study the effects of calycosin on palmitic acid-induced HepG2 cells, as well as the potential mechanisms of action.2. Potential targets of calycosin for the alleviation of insulin resistance were predicted by network pharmacology. Glucose concentration in the culture medium was determined by the GOD-POD method. The model of insulin resistance was established by palmitic acid-induced HepG2 cells. Effects of palmitic acid and calycosin on HepG2 cell activity were determined using an MTT assay kit. The expression levels of AKT1 and FOXO3a were detected by western blot.The expression level of hsa-miR-324-3p was detected by RT-qPCR. Dual luciferase reporter assay to detect targeting of AKT1 by hsa-miR-324-3p.3. AKT1 was predicted and validated as a potential target of calycosin for treatment of insulin resistance. The model of insulin resistance was successfully established by palmitic acid-induced HepG2 cells. Up-regulation of AKT1 expression inhibits FOXO3a entry into the nucleus. Calycosian was demonstrated to concentration-dependently increase the sensitivity of insulin resistance cells to insulin. The hsa-miR-324-3p was proven to exist in insulin-resistant cells. Hsa-miR-324-3p was found to target AKT1 involved in the alleviation of insulin resistance.4. Calycosin inhibits FOXO3a nuclear translocation by regulating the hsa-miR-324-3p/AKT pathway, thus alleviating insulin resistance.
研究领域:
药理学
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分子生物学
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内分泌学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Inflammation Research
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2024
·
IF 4.2
Liu Yang,Yan Xu,Jian Pan,Renjie Li,Chao Lan,Dongshan Zhang
Background Acute kidney injury (AKI) is associated with higher perioperative mortality and morbidity, as well as increased medical expenses. The molecular mechanisms underlying ischemia-reperfusion (I/R)-induced AKI remain unclear.Methods and Results We applied an RT-qPCR assay to measure the expression of mmu-lncRNA129814, hsa-lncRNA582795, and miRNA-494-5p, immunoblotting to detect IL-1α and cleaved caspase-3 expression, and TUNEL staining and flow cytometry (FCM) to evaluate apoptosis. The experiments were conducted using BUMPT and HK-2 cells, as well as C57BL/6J mice. Mechanistically, mmu-lncRNA129814 could sponge miRNA-494-5p and upregulate IL-1α expression to promote cell apoptosis. Furthermore, knockdown of mmu-lncRNA129814 ameliorated I/R-induced progression of AKI by targeting the miRNA-494-5p/IL-1α pathways. Interestingly, hsa-lncRNA582795, a homolog of mmu-lncRNA129814, also promoted I/R-stimulated HK-2 cell apoptosis and AKI progression by regulating the miRNA-494-5p/IL-1α axis. Finally, we found that patients with I/R-induced AKI exhibited significantly elevated plasma and urinary levels of hsa-lncRNA582795 compared to those who underwent ischemia-reperfusion without developing AKI. Spearman’s test demonstrated a significant correlation between serum creatinine and plasma hsa-lncRNA582795 in I/R patients. Plasma hsa-lncRNA582795 showed high sensitivity but low specificity (86.7%) compared to urinary hsa-lncRNA582795.Conclusion The mmu-lncRNA129814/hsa-lncRNA582795/miRNA-494-5p/IL-1α axis was found to modulate the progression of ischemic AKI, and hsa-lncRNA582795 could act as a diagnosis biomarker and potential therapy target of I/R-induced AKI.
研究领域:
肾病学
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分子生物学
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医学遗传学
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DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
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2022
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IF 4.446
Pengli Xiao,Shengbo Sang,Qiang Zhang,Yang Ge,Yongqiang Cheng,Dong Zhao
Currently, mechanical biosensors were greatly applied in detecting various biomarkers. However, the sensitivity of the mechanical biosensor needs to be further improved to be expected to achieve higher performance for the single molecule detection. Herein, we proposed a membrane-type biosensor based on magnetic, surface stress and electric coupling enhancement (MSEC-MBios) to detect the human serum albumin (HSA). By introducing the magnetic probes, the membrane deformation was amplified under the magnetic field, realizing the coupling of the magnetic and the traditional electromechanical mechanical biosensor system. By magnetic sensitization, the detection limit of the MSEC-MBios was reduced to 10.2 ng/mL in the linear range of 0–50 μg/mL, which was an order of magnitude lower than that of the biosensor without magnetic field. In addition, the MSEC-MBios was also proved excellent stability, selectivity, and specificity, showing potential in developing new biomolecules detection methods.
研究领域:
生物医学工程
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生物传感器
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纳米技术
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分析化学
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产品关联
DOI
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
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2024
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IF 3.5
Wenhua Tong,Shuqin Wang,Haoran Xu,Zongwei Qiao,Liming Zhao,Ying Yang
Pyrazine is a kind of compound containing pyrazine ring structure, which has attracted the attention of researchers because of its special aroma and healthcare function. It is currently used in spices, pharmaceuticals, food additives, and other industries. Human serum albumin (HSA) is a transport and storage protein in the human circulatory system. Previous research reported that pyrazine can interact with albumin, but the interaction between pyrazine and HSA has not been reported. Consequently, this study is based on the multispectral method and molecular dynamics (MD) simulation, exploring the interaction between different pyrazines and HSA. The results show that the four pyrazines mainly quench the endogenous fluorescence of HSA through the static quenching mechanism. Molecular docking indicates that hydrophobic forces play a major role in the binding of pyrazines with HSA. At the same time, synchronous fluorescence spectroscopy, circular dichroism (CD), three-dimensional fluorescence spectroscopy, and resonance light scattering (RLS) showed that each pyrazine compound can change the conformation of HSA. MD simulation results show that the combination of four pyrazines and HSA can enhance the stability of HSA. Among them, 2,3,5,6-tetramethylpyrazine (TTMP) and 2,3,5-trimethylpyrazine (TMTP) binding is the most stable and the most unstable combination with HSA, respectively. In addition, the four pyrazines could affect the physiological activity of HSA. This study is of great significance to explore the mechanism of action of pyrazine compounds in vivo and can provide useful information for the application of pyrazine compounds in food.
研究领域:
药化化学
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生物物理学
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分子生物学
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食品科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）