JOURNAL OF BIOMATERIALS APPLICATIONS
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2024
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IF 2.3
Chao Han,Dingsong Lu,Suoping Yang,Chong Liu,Feng Guo,Kai Zhang,Peng Li
The design and construction of a new and excellent synthetic graft is of great significance in the field of bone defect repair and reconstruction. In this study, a dopamine modified chitosan hydrogel doped with Cu ions with a mild photothermal effect was designed to provide a better microenvironment to advance the bone repair via promote the angiogenesis and osteogenesis. Characterizations showed the successful synthesis of the material while it also presented excellent biocompatibility and mild photothermal effect under the irradiation of near-infrared light. Further, it could enhance the angiogenesis of HUVECs cells through promoting the ability of migration and tube formation and enhance the osteogenic differentiation of MC3T3-E1 cells via increasing the content of vital osteogenic factors including Runx2, Col-1, OPN, OCN, OSX, etc. The in vivo experiment also testified that it could promote the bone defect repair in rat models. These results indicate the multifunctional hydrogel is an ideal material for the treatment of bone defects and has good clinical application potential.
研究领域:
生物材料
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组织工程
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再生医学
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生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Kairui Duan,Jiajun Hu,Yueming Zhao,Mian Lin,Mengdie Xu,Tingting Li,Bae Hoon Lee
Nonclinical toxicity testing in rodent and nonrodent models often fails to predict human pharmacological responses, and the reliance on poorly characterized biomaterials hinders clinical translation in drug development. To address these limitations, we engineered a human serum albumin methacryloyl (HSAMA) cryogel system incorporating key liver extracellular matrix (ECM) components, collagen I (C) and fibronectin (F), to mimic the liver's architectural complexity. By co-culturing HepG2 and HUVECs within the HSAMA-CF cryogel, we created a three-dimensional, large-pore scaffold that facilitates critical hepatocyte-endothelial cell interactions essential for liver function and drug metabolism. Over extended culture periods, the HSAMA-CF cryogel significantly enhanced liver-specific functions, including elevated albumin production, increased urea secretion, and upregulated expression of hepatic functional genes. Notably, the co-culture model successfully detected multiple hepatotoxic phenotypes—such as elevated transaminase levels, hepatocyte damage, and steatosis—following exposure to known hepatotoxins like fialuridine and acetaminophen. This HSAMA-CF co-culture system may represent a robust in vitro platform for liver toxicity assessment. By closely mimicking human liver physiology and drug responses, it might offer a promising alternative to traditional animal models, potentially improving drug safety evaluations, reducing reliance on preclinical animal testing, and minimizing risks associated with clinical drug development.
研究领域:
生物医学工程
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药理学
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毒理学
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组织工程
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药物研发
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细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
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2024
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IF 6.1
Shubin Shi,Mengyue Hu,Xu Peng,Can Cheng,Shaoxiong Feng,Xinyun Pu,Xixun Yu
Due to the increasing aging population and the advancements in transcatheter aortic valve replacement (TAVR), the use of bioprosthetic heart valves (BHVs) in patients diagnosed with valvular disease has increased substantially. Commercially available glutaraldehyde (GA) cross-linked biological valves suffer from reduced durability due to a combination of factors, including the high cell toxicity of GA, subacute thrombus, inflammation and calcification. In this study, the oxidized chondroitin sulfate (OCS), a natural polysaccharide derivative, was used to replace GA to cross-link decellularized bovine pericardium (DBP), carrying out the first crosslinking of DBP to obtain OCS-BP. Subsequently, in situ the zwitterion radical copolymerization system was introduced to perform double cross-linking to obtain double crosslinked BHVs with biomimetic modification (P(APM/MPC)-OCS-BP). P(APM/MPC)-OCS-BP presented enhanced mechanical properties, collagen stability and enzymatic degradation resistance owing to double crosslinking. The ex vivo AV-shunt assay and coagulation factors test suggested that P(APM/MPC)-OCS-BP exhibited excellent anticoagulant and antithrombotic properties due to the introduction of P(APM/MPC). P(APM/MPC)-OCS-BP also showed good HUVECs-cytocompatibility due to the substantial reduction of its residual aldehyde group. The subcutaneous implantation also demonstrated that P(APM/MPC)-OCS-BP showed a weak inflammatory response due to the anti-inflammatory effect of OCS. Finally, in vivo and in vitro results revealed that P(APM/MPC)-OCS-BP exhibited an excellent anti-calcification property. In a word, this simply cooperative crosslinking strategy provides a novel solution to obtain BHVs with good mechanical properties, HUVECs-cytocompatibility, anti-coagulation, anti-inflammatory and anti-calcification. It might be a promising alternative to GA-fixed BP and exhibited a good prospect in the clinical applications.
研究领域:
心血管工程
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生物材料科学
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组织工程
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医疗器械
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）