Marine Drugs
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2022
·Vol. 20
·期 5
·284
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IF 6.085
Yuting Zhu,Shanhao Han,Jianhui Li,Hongwei Gao,Bo Dong
Marine ascidian is becoming one of the main sources of an antitumor drug that has shown high bioactivity and extensive application in cancer treatment.Halocynthia roretzi, an edible marine sea squirt, has been demonstrated to have various kinds of biological activities, such as anti-diabetic, anti-hypertension, and enhancing immunity. In this study, we reported that aqueous extracts from the edible parts ofH. roretzipresented significantly inhibiting the efficiency on HepG-2 cell viability. The separate mixed compound exhibited strong effects of inhibitory proliferation and induced apoptosis via the generation of ROS along with the concurrent loss of mitochondrial membrane potential on tumor cells. Furthermore, we found that there existed a significantly synergistic effect of the ascidian-extracted compound mixture with the anti-cancer drug doxorubicin. In the presence of the extracts fromH. roretzi, the dose of doxorubicin at the cellular level could be reduced by a half dose. The extracts were further divided by semipreparative-HPLC and the active ingredients were identified as a mixture of fatty amide, which was composed of hexadecanamide, stearamide, and erucamide by UHPLC-MS/MS. Our results suggest that the potential toxicity of ascidianH. roretziin tumor cells, and the compounds extracted fromH. roretzicould be potentially utilized on functional nutraceuticals or as an adjunct in combination with chemotherapy.Keywords:ascidian;Halocynthia roretzi;anti-tumor;drug combination;doxorubicin
研究领域:
海洋生物学
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肿瘤学
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药理学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2022
·Vol. 13
·期 11
·6008-6021
·
IF 6.317
Rui Ma,Qiqi Chen,Yanping Dai,Yan Huang,Qing Hou,Yuting Huang,Kai Zhong,Yina Huang,Hong Gao,Qian Bu
Ocean life contains a wealth of bioactive peptides that could be utilized in nutraceuticals and pharmaceuticals. This study aimed to obtain neuroprotective antioxidant peptides in sea squirt (Halocynthia roretzi) through protamex enzymolysis. Fraction F4 (ultrafiltration generated four fractions) had a lower molecular weight (<500 Dalton (Da)) with greater 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical scavenging activities (94.24 ± 2.50% and 91.80 ± 1.19%). After gel filtration, six peptides, including Phe-Gly-Phe (FGF), Leu-Gly-Phe (LGF), Leu-Phe-VAL (LFV), Val-Phe-Leu (VFL), Trp-Leu-Pro (WLP), and Ile-Ser-Trp (ISW), were identified and sequenced by liquid chromatography-mass spectrometry (LC-MS/MS). Peptides WLP and ISW showed higher oxygen radical absorbance capacity (ORAC) values (2.72 ± 0.47 and 1.93 ± 0.01 μmol L−1 of Trolox equivalent (TE) per μmol L−1 of peptide) than glutathione (GSH). Additionally, WLP effectively increased cell viability, dramatically attenuated 6-Hydroxydopamine (6-OHDA)-induced cell apoptosis and decreased reactive oxygen species (ROS) levels to nearly two-fold, and significantly boosted glutathione peroxidase (GSH-Px) activity in PC12 cells. Transcriptome sequencing revealed differential expression of genes associated with various oxidative stress pathways after WLP treatment, such as glutathione metabolism. These results suggest that the Halocynthia roretzi-derived tripeptide WLP could alleviate neurodegenerative diseases associated with oxidative stress.
研究领域:
海洋生物学
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药理学
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神经科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Materials Today Bio
·
2024
·
IF 8.7
Cuicui Ding,Kuan Cheng,Yue Wang,Yifan Yi,Xiaohong Chen,Jingyi Li,Kaiwen Liang,Min Zhang
Recently, biomacromolecules have received considerable attention in hemostatic materials. Collagen, an ideal candidate for hemostatic sponges due to its involvement in the clotting process, has been facing challenges in extraction from raw materials, which is time-consuming, expensive, and limited by cultural and religious restrictions associated with traditional livestock and poultry sources. To address these issues, this study explored a new shortcut method that using wild Halocynthia roretzi (HR), a marine fouling organism, as a raw material for developing HR collagen fiber sponge (HRCFs), which employed urea to disrupt hydrogen bonds between collagen fiber aggregates. This method simplifies traditional complex manufacturing processes while utilized marine waste, thus achieving dual green in terms of raw materials and manufacturing processes. FTIR results confirmed that the natural triple-helical structure of collagen was preserved. HRCFs exhibit a blood absorption ratio of 2000–3500 %, attributed to their microporous structure, as demonstrated by kinetic studies following a capillary model. Remarkably, the cytotoxicity and hemolysis ratio of HRCFs are negligible. Furthermore, during in vivo hemostasis tests using rabbit ear and kidney models, HRCFs significantly reduce blood loss and shorten hemostasis time compared to commercial gelatin sponge and gauze, benefiting from the capillary effect and collagen's coagulation activity. This study provides new insights into the design of collagen-based hemostatic biomaterials, especially in terms of both raw material and green manufacturing processes.
研究领域:
生物医学工程
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海洋生物学
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材料科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Cuicui Ding,Yifan Yi,Kuan Cheng,Yue Wang,Shaoyun Wang,Min Zhang
The extraction of collagen for packaging films typically requires a time-consuming process and the use of substantial chemicals. Herein, we present a full life cycle green preparation method for rapidly producing collagen-based food packaging films using Halocynthia roretzi (HR), a collagen-rich marine organism, as raw material. We first prepared the micro/nano-sized collagen fibers from HR tissue by utilizing urea and sonication as effective hydrogen-bond breakers. Subsequently, the collagen fiber was rapidly fabricated into a film through vacuum filtration. The resulting collagen fiber film (CFF) exhibited a uniform and dense surface, along with good tensile properties , water resistance, and biodegradability. In addition, the deposition of chitosan (CS) on the surface of CFF resulted in a remarkable preservation effect for both strawberries and pork. This full life cycle preparation method for collagen-based films provides a promising and innovative approach to the sustainable preparation of food packaging films.
研究领域:
生物材料学
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海洋生物学
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食品科学
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产品关联
DOI
PubMed
高影响因子
ENERGY & FUELS
·
2024
·
IF 5.2
Yanyan Niu,Xin Fu,Yifei Zhu,Fanjun Meng,Qinglin Ma,Cui Song,Qian Zhang,Yuju Che
As a “green” displacing agent, nanocellulose is enriched, nontoxic, biodegradable, and environmentally friendly; therefore, the development and potential application of nanocellulose in oilfield chemistry have attracted widespread attention in recent years. In this study, a thermothickening copolymer synthesized in our laboratory called hydroxypropyl methyl cellulose-based graft (PHAD) copolymer was chosen to mix with Halocynthia roretzi Drasche-based cellulose nanocrystals (TCNCs), and anionic surfactant sodium dodecyl sulfate (SDS) was added to improve the stability of the displacing agent. After the optimal SDS concentration was confirmed, PHAD/TCNCs/SDS dispersions containing 0.05–0.15 wt % TCNCs can be stable for a long time. Hence, the rheological performance of dispersions containing 0.05–0.15 wt % TCNCs was investigated systematically, and the results revealed that the PHAD/TCNCs/SDS dispersions had outstanding thermothickening ability, salt resistance, shear resistance, and viscoelastic property in a simulated oil reservoir circumstance, and the interaction mechanism among PHAD, TCNCs, and SDS was analyzed. Finally, a core flooding test was conducted, and the obtained data showed that the oil recovery of dispersion was 21.5%, which is higher than the control dispersion without TCNCs, confirming that the PHAD/TCNCs/SDS dispersions have significant practical application prospects.
研究领域:
石油化学
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材料科学
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环境科学
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产品关联
DOI
高影响因子
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