Sustainable polyhydroxybutyrate (PHB) production from biowastes by Halomonas sp. WZQ-1 under non-sterile conditions
Polyhydroxyalkanoates (PHA) are promising candidates for replacing petroleum-derived plastics; however, their high production costs limit their commercialisation. In this study, we successfully isolated an efficient PHA-producing strain from a salt lake, which was subsequently identified as Halomonas sp. WZQ-1. Notably, Halomonas sp. WZQ-1 could serve as a promising cell-factory platform for polyhydroxybutyrate (PHB) production, achieving a comparatively high PHB productivity (7.64 ± 0.4 g L −1 ) under moderate salt stress (60 g L −1 NaCl). We further realised semi-continuous PHB production in a bench-scale fermenter at a steady state by irregularly replenishing the organic substrate. The maximum PHB concentration reached 12.13 g L −1 . Finally, we realised the non-sterile conversion of typical biowastes (e.g. pomelo and cantaloupe residues) to PHB using Halomonas sp. WZQ-1. Encouragingly, 4.36 g L −1 PHB was directly obtained from the hydrolysate of pomelo residues with a characteristic melting temperature of 174.0 °C. Life cycle assessment was employed to systematically evaluate the environmental sustainability and potential challenges of biowaste-driven PHB biorefineries. Overall, our findings could serve as a pivotal step toward the commercialisation of PHB and provide a valuable reference for PHB biorefineries.
危险品化学品经营许可证（带存储）