Bioengineered
·
2022
·
IF 6.832
Wei-Hao Wu,Xiao-Mei Ma,Jian-Qing Huang,Qin Lai,Fu-Neng Jiang,Cui-Yun Zou,Long-Tian Chen,Lian Yu
This study investigated whether CRISPR/Cas9 (D10A) nickase-mediated gene editing can correct the aberrant Hb Constant Spring mutation (Hb CS or HBA2: c.427 T > C) in fibroblasts. Vectors for repairing the α-globin-encoding gene, HBA2:c.427 T > C mutation, includingthe CRISPR/Cas9(D10A)-sg plasmid and donor with homology arms, were constructed and used to perform gene editing in patient-derived fibroblasts. We subsequently analyzed the genetic correction, the gene editing efficiency and off-target effect. Sequencing analysis and the BamHI assay showed that HB CS mutant cells were repaired with Hb CS point mutations, the editing efficiency was 4.18%~9.34% and no off-target effects were detected. The results indicate that the HB CS mutant gene is effectively repaired by the CRISPR/Cas9 (D10A)system, which may enable truly personalized therapy for precise repair of α-thalassemia.
研究领域:
分子生物学
·
遗传学
·
医学
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产品关联
DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Juan Liu,Wenju Weng,Chunxiao Yin,Guiling Luo,Hui Xie,Yanyan Niu,Xiaoyan Li,Guangjiu Li,Yaru Xi,Yutong Gong,Shuyao Zhang,Wei Sun
In this paper zinc-based metal organic framework material (ZIF-8) modified carbon ionic liquid electrode (CILE) was prepared and electrodeposited with gold nanoparticles to get a composite modified electrode, which was used as the sensing interface for the immobilization of hemoglobin (Hb) with Nafion to prepare a modified electrode (Nafion/Hb/Au/ZIF-8/CILE). Electrochemical behaviors of Hb on the modified electrode were checked by cyclic voltammetry with a pair of well-defined redox peaks appeared. The effects of pH and scan rate on direct electrochemistry of Hb were studied with electrochemical parameters calculated. The prepared electrochemical enzyme sensor showed good catalytic activity to the electroreduction of bromate and nitrite with the linear ranges from 0.5 to 10.0 mmol/L and from 0.1 to 0.8 mmol/L, respectively. The detection limits were calculated as 0.83 mmol/L and 0.03 mmol/L, and the water samples were analyzed to prove the practical application.
研究领域:
生物化学
·
分析化学
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产品关联
DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 97
·95199-95203
·
IF 3.289
Shi-Yu Lu,Sheng-Hui Liao,Shu-Juan Bao,Meng Jin,Bo Weng,Chang-Ming Li
The excellent selectivity and anti-interference ability of enzyme-based biosensors have attracted considerable attention by researchers. However, the short lifetime, difficult electron transfer, and easily leak from the supporting materials restrict the practical applications of enzyme. In this study, an enzyme–inorganic hybrid biosensor constructed from slack Hb–Mn3(PO4)2·3H2O nanosheets was prepared by a simple and effective in situ immobilization method. Designed as an H2O2 sensor, it exhibits a fast electron transfer rate constant (ks = 4.16 s−1), an ultra-wide linear range for H2O2 detection (20 to 56 100 μM), and long life-time (85% of initial response after 29 days), which may be attributed to the effective immobilization of the enzyme and the good biocompatibility of Mn3(PO4)2·3H2O. For the detection of a real sample, the range of recovery of our designed biosensor was around 98.2–102.6%, confirming that our proposed method presents a promising immobilization enzyme-based biosensor for use in practical applications.
研究领域:
生物化学
·
分析化学
·
材料科学
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DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Li Ding,Yiwen Xie,Xinrui Bai,Liang Qiu,Zharykbasov E,Z. Kakimova,Gang Wu,Jianlong Wang,Yuxia Song,Rong Wang
Here, we report a general ultrasound-assisted photodynamic strategy that overcomes this challenge by employing hypocrellin B (HB), originally a narrow-spectrum agent initially effective against Gram-positive bacteria, to eradicate Gram-negative bacteria. By applying exogenous ultrasound (US), we induce interfacial electron movement and leverage the cavitation effect to disrupt the lipid bilayer structure of the outer membrane (OM). Consequently, 200 μg mL −1 of HB@ZIF-8 (ZHB) achieves an almost 7-log reduction in Escherichia coli counts within 20 min, which is sixfold higher compared to the antibacterial activity exhibited by HB alone. Molecular dynamics simulations combined with 1.3-fold increase in fluorescence signals in OM permeability experiments confirm that the synergy of ultrasound and photodynamic treatment promotes OM permeability, facilitating rapid diffusion of ROS and HB into inner membrane and intracellular regions. Furthermore, the ZHB exhibited superior inhibition of bacterial proliferation, remarkably extending Shine Muscat grapes shelf life by up to 15 days. Therefore, the sonodynamic interfacial-assisted photosensitizer ZHB prepared in this work was found effective in enhancing the antimicrobial effect against foodborne pathogens.
研究领域:
纳米技术
·
抗菌治疗
·
生物物理学
·
食品科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2019
·
IF 3.218
Yue Sun,Siyu Li,Yifei Yang,Xuewei Feng,Wei Wang,Yutong Liu,Mengyuan Zhao,Zhiguang Zhang
A new kind of thermal responsive protein imprinted polymers (TPIPs) was prepared for the electrochemical biosensor. The TPIPs were synthesized on the surface of zinc oxide (ZnO) nanoflowers via electrochemically mediated atom transfer radical polymerization (eATRP), with bovine hemoglobin (Hb) as both catalyst and template. N-isopropyl acrylamide (NIPAM) and methacrylic acid (MAA) were used as temperature sensitive and assistant functional monomer , respectively. The biosensor fabricated by TPIPs and ZnO was successfully used for detecting Hb by differential pulse voltammetry (DPV). Under suitable conditions, the detection range of Hb was from 1.0 × 10 −13 to 1.0 × 10 −1 mg·L −1 with a low detection limit of 3.1 × 10 −14 mg·L −1 (S/N = 3). This biosensor was used for detecting bovine hemoglobin in real samples with the recoveries of 95–105%. The good temperature response, good selectivity and repeatability allowed the TPIPs to be a great importance for the polymer-modified electrodes and the sensitive detection of Hb.
研究领域:
生物传感器
·
电化学
·
纳米技术
·
高分子科学
·
分析化学
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DOI
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2021
·Vol. 25
·期 5
·1645-1655
·
IF 2.647
Bo Shuang,Sun Yue,Li Siyu,Zhou Yuxi,Feng Xuewei,Li Cui
A novel hemoglobin (Hb) imprinted polymers with CO 2 response (CHIPs) were prepared on the surface of Au electrode modified by zinc oxide (CHIPs/ZnO/Au) via electrochemically mediated atom transfer radical polymerization (eATRP). The imprinted polymers were synthesized with Hb both as catalyst and template, and N,N-diethylaminoethyl methacrylate (DEAEMA) as CO 2 -responsive functional monomer. The obtained CHIPs/ZnO/Au electrode was characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and electrochemical methods. The results showed that CHIPs/ZnO/Au electrode could give higher affinity for the target protein inside a CO 2 -treated aqueous medium compared to that under N 2 -treated conditions, which make it a good electrochemical biosensor in the presence of CO 2 . The biosensor could be successfully applied for the determination of Hb by differential pulse voltammetry (DPV) measurement. The linear response range of CHIPs/ZnO/Au for detecting Hb was 1.0×10 −12 ~1.0×10 −1 mg L −1 and the detection limit was 2.1×10 −13 mg L −1 ( S / N =3). Briefly, the work in this study introduced an easy and non-toxic method for Hb determination under specific conditions and also provided a progressive approach for designing protein imprinted polymers.
研究领域:
材料科学
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分析化学
·
生物传感器
·
电化学
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产品关联
DOI
高影响因子
Artificial Cells Nanomedicine and Biotechnology
·
2018
·
IF 3.026
Donglai Qi,Qian Li,Chen Chen,Xiang Wang
During the development of artificial red blood cell (RBC) substitutes, oxidation side reaction is one of the major factors that hinder the application of haemoglobin (Hb)-based oxygen carriers (HBOCs). In order to avoid oxidation toxicity, we designed and prepared natural Hb conjugated with ferulic acid (FA) via simple chemical modification. In addition, the thiol groups on Hb surface were increased via the reaction of Hb with 2-iminothiolane (2-IT) and then modified with FA for the study of anti-oxidant ability. It was showed that Hb modified with FA (FA-Hb) had similar oxygen-binding capacity to natural Hb. Moreover, the anti-oxidant ability of FA-Hb in vitro in different systems was superior to natural Hb and in proportion to the degree of modification of FA. The results indicate that FA-Hb might have the potential to serve as a novel oxygen carrier with the capacity to reduce oxidative side reaction.
研究领域:
生物化学
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医学工程
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产品关联
DOI
PubMed
高影响因子
MACROMOLECULAR CHEMISTRY AND PHYSICS
·
2023
·Vol. 224
·期 23
·2300304
·
IF 2.5
Mingzhao Li,Jia Wang,Anjun Qin,Ben Zhong Tang
Hyperbranched polymers have been widely applied in various fields because of their good solubility, high reactive end groups, and low viscosity, etc. Therefore, it is of great importance to develop new strategies to build multifunctional hyperbranched polymers with novel architectures. In this work, a C(sp 3 )─H activation-based polymerization of internal alkynes and bifunctional secondary amines is successfully established, and multifunctional hyperbranched polyamines (hb-PAs) with 100% E -isomers and high molecular weights ( M w up to 76 500) are prepared in good yields (up to 82%). The hb-PAs containing tetraphenylethylene moiety show unique aggregation-enhanced emission properties and can be used for explosive detection with a low limit of detection (9.14 × 10 −8 m ). Notably, the hb-PAs display high refractive indices (up to 1.7607) that can be further modulated to 1.7825 by the coordination of its peripheral ethynyl group with metallic cobalt. Moreover, the hyperbranched structure endows the hb-PAs with much better properties than their linear counterparts, such as higher photoluminescence quantum yields and refractive indices. In addition, the hb-PAs can be modified by a C(sp 3 )─H activation reaction of internal alkynes and alcohols. This work not only prepares a series of functional nitrogen-containing hyperbranched polymers but also promotes the application of C(sp 3 )─H activation in polymer chemistry.
研究领域:
高分子化学
·
材料科学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2025
·
IF 2.8
Yinshuo Xu,Yuanjie Shan,Yuta Nishio,Kenichiro Todoroki,Toshimasa Toyo'oka,Toufeng Jin,Jun Zhe Min
With the development of untargeted metabolomics, studies have shown that alterations in the serum levels of specific amino acids are strongly correlated with the development of early-stage colorectal cancer (CRC). However, the relationship between optically active free amino acids in human nails and the risk of CRC remains unclear. In this study, a highly sensitive UHPLC-MS/MS chiral separation method based on ( S )-(−)-DBD-Pro-COCl derivatization was established for the simultaneous detection of DL-Asp, DL-Kyn, DL-α-hydroxybutyric acid (DL-α-HB), and Cystamine in the fingernails of patients with CRC. The derivatization reagents were incubated with DL-amino acids, DL-α-HB and Cyst at 60 °C for 15 min to form diastereomers. Subsequently, an Imtakt cadenza CD-C 18 column (3.0 × 150 mm, 3.0 μm) was used for the separation of diastereomers under gradient elution of 10 mM HCOONH 4 in H 2 O as mobile phase A and 0.1 % HCOOH in CH 3 CN as mobile phase B. The degrees of separation (Rs) ranged from 1.76 to 3.21. R 2 was >0.9995, indicating good linearity; the limit of detection (LOD) was 25–100 fmol; the interday and intraday precision of DL-Asp and DL-α-HB detection were 0.85 %–4.91 %; and the average recovery ranged from 81.12 % to 110.2 %. However, during the determination process of fingernail samples, only DL-Asp and DL-α-HB were successfully detected, while DL-Kyn and Cyst were not detected. Therefore, this novel method was used to quantify DL-Asp and DL-α-HB in fingernail samples from 32 healthy volunteers (HVs) and 16 patients with CRC (CPs). The results revealed significant differences in D-Asp ( p < 0.05), L-Asp ( p < 0.01), and L-α-HB ( p < 0.05) levels between male HVs and CPs and in D-α-HB ( p < 0.05) levels between female HVs and CPs. In addition, the D/L-α-HB ratio ( p < 0.01) was significantly different between male HVs and CPs. These findings validate the use of DL-amino acids, DL-α-HB and Cyst from human fingernails as potential diagnostic biomarkers for CRC, providing a basis for the clinical application of human fingernail samples for the noninvasive diagnosis of CRC.
研究领域:
分析化学
·
癌症研究
·
代谢组学
·
临床诊断
·
手性分离
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DOI
PubMed
高影响因子
APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
·
2019
·Vol. 125
·期 9
·1-6
·
IF 1.784
Zhao Tian,Dong Ming,Zhao Yi
[Fe(HB(pz) 3 ) 2 ], a well-studied spin-crossover (SCO) complex, has been entrapped in NH 2 -MIL-101(Al) metal–organic framework (MOF) via gas diffusion method. [Fe(HB(pz) 3 ) 2 ] would sublimate when temperature exceeds 100 °C, especially at low pressure. Thus, in the selected condition, [Fe(HB(pz) 3 ) 2 ] molecules can be sublimated to a gas phase and diffused into the pores of NH 2 -MIL-101(Al), which yield [Fe(HB(pz) 3 ) 2 ]@MIL-101 hybrid materials. The identity of composites, with a loading of iron complex at ~ 8 wt%, was characterized by X-ray diffraction and spectroscopic attestation. The hybrid materials demonstrate a gentle spin transition curve from 300 to 400 K, which is different from the SCO behavior of [Fe(HB(pz) 3 ) 2 ]. And for the pure [Fe(HB(pz) 3 ) 2 ] sample at 400 K, only 60% of Fe(II) is in HS, while in hybrid samples, HS state is complete. This interesting phenomenon might indicate that the spin transition in the hybrid sample can be triggered at lower temperature. The composite samples were thoroughly studied by X-ray diffraction, IR spectroscopy, atom absorption spectroscopy, nitrogen physisorption, and magnetic measurements. Thereby, a novel MOF-based material with isolated SCO units is proposed, which demonstrate a salient ‘matrix-effect’ on spin-crossover behavior of [Fe(HB(pz) 3 ) 2 ].
研究领域:
材料科学
·
化学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Yuchu He,Cong Cong,Lei Li,Liyao Luo,Yaqian He,Zining Hao,Dawei Gao
Cells in the center of solid tumors have always been an abyss untouched by treatments because of their deep location and increased drug resistance. Herein, we designed a rational strategy for sequential intra-intercellular delivery of nanomedicine to deep sites of drug-resistant solid tumors. In our formulation, dopamine and hemoglobin were polymerized to form a smart nanocarrier (PDA/Hb). Subsequently, the doxorubicin and nitric oxide donor were connected on the surface of PDA/Hb to obtain D/N-PDA/Hb. Ultimately, the hyaluronic acid was combined with D/N-PDA/Hb to form D/N-PDA/Hb@HA. Concretely, acidic and neutral environments of tumor cells were treated as a switch to turn on or off the drug release of a nanodrug. Meanwhile, the generation of nitric oxide in situ was exploited to favor the lysosomal escape of nanocarriers and overcome the drug resistance of deep solid tumor cells. The results indicated that the nanodrug based on sequential intra-intercellular delivery showed exciting penetration efficiency and resistance reversal of solid tumors. Conventional nanodrug delivery was highly dependent on the enhanced permeability and retention (EPR) effect and limited by tumorous interstitial fluid pressure. Plenty of drugs stayed on the surface of solid tumors, and the infiltrated drugs were inefficient due to strict resistance. To conquer this dilemma, this work proposed a new mechanism reversing the EPR effect for drug delivery, leading to better penetration and resistance reversal of solid tumors.
研究领域:
癌症研究
·
纳米技术
·
药学
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMERS AND THE ENVIRONMENT
·
2024
·Vol. 32
·期 7
·3142-3158
·
IF 4.7
Mo Yanping,Cheng Zhiru,Xie Yuhui,Chu Junhan,Xie Shuangyu,Meng Yang,Wu Feng,Feng Dong,Mei Yi,Xie Delong
Flexible polyurethane foam (FPUF) has a widespread application across aerospace, furniture and vehicles, while its flammability always arouses severe safety concerns. Herein, a novel, bio-based flame retardant, i.e. polyol (PADEA) that contains phosphorus and nitrogen elements, was successfully synthesized by using phytic acid (a bio-organic acid) and diethanolamine as crude materials. Then, PADEA partially substituted the commercial polyether polyols in the foaming process, during which PADEA reacted in situ with isocyanate to construct an inherently flame-retardant FPUF. Due to the purposive designed P-N synergistic molecular structure, PADEA can serve as a highly efficient intumescent flame-retardant (IFR) system. When 40 php (ca. 21.51wt%) PADEA was added into the system, the limiting oxygen index (LOI) of the obtained FPUF reached up to 24.1%, achieving a 22.0% increase compared to that of primitive FPUF without PADEA. Moreover, the addition of PADEA can considerably reduce the total number of melt drips in the UL-94 HB rating test. And the cone calorimeter (CC) results demonstrated a 47.08% reduction in total heat release (THR) was achieved as 40 php PADEA was added. At the end, various characterization methods were carried out, revealing the flame-retardant mechanism of PADEA both in condense and gaseous phase. This work highlights a facile and green strategy for designing functional polyol to address the flame-retardant issues of high-performance FPUF. Graphical
研究领域:
高分子科学
·
材料工程
·
消防安全工程
·
绿色化学
·
化学合成
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DOI
高影响因子
危险品化学品经营许可证（带存储）