RSC Advances
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2021
·Vol. 11
·期 17
·10264-10271
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IF 3.361
Xinyu Wang,Zhuo Li,Jiaojiao Nie,Liangqiang Wu,Weihong Chen,Shaolong Qi,Hai Xu,Jianshi Du,Yaming Shan,Qingbiao Yang
Copper is an essential element in living systems and plays an important role in human physiology; therefore, methods to detect the concentration of copper ions in living organisms are important. Herein, we report a highly water-soluble naphthalimide-based fluorescent probe that can be used for the detection of Cu2+. The probe, BNQ, has high selectivity and sensitivity. The fluorescence intensity of the probe at 520 nm was visible to the naked eye under a UV lamp; upon the gradual addition of Cu2+, there was a colour change from green to nearly colourless. Furthermore, the detection limit of BNQ for Cu2+ was 45.5 nM. The detection mechanism was investigated using a Job's plot and density functional theory (DFT) calculations. In addition, owing to great biocompatibility, we were able to successfully use BNQ to detect Cu2+ in living HeLa cells with low toxicity.
研究领域:
生物化学
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分析化学
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细胞生物学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2021
·
IF 7.46
Yinghua Guo,Xinru Wang,Yan Feng,Xiao Luo,Xiangming Meng,Xuhong Qian,Youjun Yang
Autophagy is a physiological process governing proteostasis and implicated in many diseases. Enhanced ROS levels was known to be associated with lysosomal autophagy and therefore a lyso-specific ROS probe could find applications in autophagy related studies. Following the probe-design principle of bridging-group modification, we previously developed a ROS probe capable of detect a broader range of reactive species than the existing probes. Additional merits of the probe include ratiometry-capability, minimal probe-product spectral crosstalk, resistance toward autoxidation at lysosomal acidity, and structural simplicity. It is the aim of this manuscript to render this probe lyso-specificity and examine its potentials to monitor autophagy-related ROS generation in HeLa cells treated by H 2 O 2 or 6-OHDA.
研究领域:
细胞生物学
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生物化学
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医学研究
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2018
·
IF 5.667
Linlin Hu,Xing Wei,Jie Meng,Xiaoyan Wang,Xuwei Chen,Jianhua Wang
The development of fluorescent probes for detection of glutathione (GSH) has attracted extensive attentions due to its close association with cellular functions and diseases. Herein, a fluorescence enhancement sensing system based on acetaldehyde-modified-cystine (AMC) is reported for the detection of thiols, providing detection limits of 36 and 52 μmol L −1 for GSH and cysteine (Cys) respectively. Importantly, the fluorescence response of AMC is highly selective toward thiols with respect to the coexisting species in real biological matrixes. Considering the fact that GSH levels in cancer cells is significantly higher than those of Cys and other reducing species, the fluorescence enhancing response of AMC in cancer cells is dominated by GSH. Therefore, this provides the possibility for selective intracellular GSH imaging in living cancer cells, i.e., HeLa cells in the present study.
研究领域:
生物化学
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医学诊断
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细胞生物学
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DOI
高影响因子
JOURNAL OF LUMINESCENCE
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2014
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IF 2.367
Xing-Pei Ye,Shao-bo Sun,Ying-dong Li,Li-hua Zhi,Wei-na Wu,Yuan Wang
A new o -aminophenol-based fluorogenic chemosensor methyl 3,5-bis(( E )-(2-hydroxyphenylimino)methyl)-4-hydroxybenzoate 1 have been synthesized by Schiff base condensation of methyl 3,5-diformyl-4-hydroxybenzoate with o -aminophenol, which exhibits high selectivity and sensitivity toward Al 3+ . Fluorescence titration studies of receptors 1 with different metal cations in CH 3 OH medium showed highly selective and sensitive towards Al 3+ ions even in the presence of other commonly coexisting metal ions. The detection limit of Al 3+ ions is at the parts per billion level. Interestingly, the Al(III) complex of 1 offered a large Stokes shift (>120 nm), which can miximize the selfquenching effect. In addition, possible utilization of this receptor as bio-imaging fluorescent probe to detect Al 3+ in human cervical HeLa cancer cell lines was also investigated by confocal fluorescence microscopy .
研究领域:
生物化学
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分析化学
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细胞生物学
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DOI
高影响因子
Chemistry Central Journal
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2018
·Vol. 12
·期 1
·1-7
·
IF 2.284
Jin Guofan,Xiao Fuyan,Liu Ruijiang
(Z,Z’)-1,1′-(4-ortho-Caboranyldimethyl)-bis(2-methoxyphenylethan-1-oxime) intermediate 3 was synthesized by a three-step reaction with a final treatment with base to give a new series of ortho-carboranyl biphenyloxime derivatives (4–8). Compounds 7 and 8 showed high solubility and the in vitro study results revealed high levels of accumulation in HeLa cells with higher cytotoxicity and boron uptake compared to l-boronphenylalanine.
研究领域:
药理学
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肿瘤学
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产品关联
DOI
PubMed
高影响因子
INORGANIC CHEMISTRY
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2015
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IF 4.762
Jing Liu,Jian Liu,Weisheng Liu,Haoli Zhang,Zhengyin Yang,Baodui Wang,Fengjuan Chen,Haotai Chen
The combination of different fluorescent species into one nanostructure to develop fluorescent nanoparticles with multiple emission signatures by a single wavelength excitation has become a very popular research area in the field of multiplex bioanalysis, diagnostics, and multicolor imaging. However, these novel hybrids must be elaborately designed to ensure that the unique properties of each component are conveyed, i.e., fluorescent species and nanoparticles, and are maximized without serious interactions with each other. Herein, a first triple-fluorescence dumbbell nanoprobe with large Stokes shift based on incorporating fluorescein isothiocyanate (FITC) and lanthanide complexes onto Au–Fe3O4 NPs was synthesized. This hybrid displays well-resolved triple fluorescence emission, with FITC at 515 nm, Tb(III) complex at 545 nm, and Eu(III) complex at 616 nm under a single-excitation wavelength and is used for highly selective and sensitive colorimetric detection of Cu2+ with a detection limit of 30 nM. Under different Cu2+ concentrations, this hybrid exhibited distinguishable multiple colors under UV light, and the color could change in the presence of different concentrations of Cu2+. This sensor for ratio/multianalyte microscopic imaging of Cu2+ in HeLa cells and BHK cells was also demonstrated. Target molecules, such as folic acid, can be covalently attached to the fluorescent nanoparticle surface to serve as an effective probe for simultaneous multicolor imaging folate receptor-overexpressing HeLa cell lines in vitro.
研究领域:
生物分析
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诊断学
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成像技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
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2018
·Vol. 107
·期 6
·1786-1791
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IF 3.373
Yujing You,Chenhao Tang,Gang Zhang,Zhiqiang Jiang,Zhongda Lv
Cell growth and release from the hydrogel scaffolds are critical steps in cell culture and cell therapies. Herein a double network hydrogel with one thermosensitive P(AEtMA-Cl-DEAEA) network and the other polyacrylamide network is reported. The diad hydrogel is elastic and maintains its structural integrity under large deformation. BSA is adsorbed onto the hydrogel at body temperature and subsequently released from the hydrogel at lower temperature. Hela cells proliferate on the hydrogel scaffolds at 37°C. When the scaffold is incubated at low temperature (20°C), 32.6% ± 6.4% of its population are detached from the scaffolds. The detached cells remain active in cell culture. This hydrogel with excellent mechanical properties and unique biological function has promising biomedical applications. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1786–1791, 2019.
研究领域:
生物材料学
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细胞生物学
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组织工程学
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2021
·Vol. 413
·期 15
·3945-3953
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IF 4.157
Yang Jin,Chen Weixing,Chen Xinyu,Zhang Xi,Zhou Hongwei,Du Haotian,Wang Mingcheng,Ma Yiting,Jin Xilang
Novel spherical polymer nanoparticles were synthesized by hyperbranched polyethylenimine (hPEI) and 6-hydroxy-2-naphthaldehyde (HNA) via Schiff base reaction (one-pot reaction), which had great advantages in water solubility and green synthesis. Meanwhile, probe PEI-HNA could quickly detect Cu 2+ in the range of 0–60 μM in 30 s with the detection limit of 243 nM. The fluorescence of PEI-HNA-Cu 2+ could be recovered by the addition of S 2− in 50 s with the detection limit of 227 nM. Based on the excellent optical properties, PEI-HNA has been used in the bioimaging of living cells with excellent cell penetrability and low toxicity. More importantly, PEI-HNA has been doped into filter paper, hydrogel, and nanofibrous film to prepare solid-phase sensors, displaying rapid response and excellent sensitivity. Moreover, the low-cost and simple preparation of these sensors offers great potential and possibilities for industrialization, which could help accelerate the development of sensors in environmental and biological fields.
研究领域:
分析化学
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生物医学工程
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材料科学
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产品关联
DOI
PubMed
高影响因子
Scientific Reports
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2018
·Vol. 8
·期 1
·1-12
·
IF 4.122
Farooq Muhammad U.,Novosad Valentyn,Rozhkova Elena A.,Wali Hussain,Ali Asghar,Fateh Ahmed A.,Neogi Purnima B.,Neogi Arup,Wang Zhiming
Colloidal gold nanoparticles (AuNPs) are of interest as non-toxic carriers for drug delivery owing to their advanced properties, such as extensive surface-to-volume ratio and possibilities for tailoring their charge, hydrophilicity and functionality through surface chemistries. To date, various biocompatible polymers have been used for surface decoration of AuNPs to enhance their stability, payloads capacity and cellular uptake. This study describes a facile one-step method to synthesize stable AuNPs loaded with combination of two anticancer therapeutics, -bleomycin and doxorubicin. Anticancer activities, cytotoxicity, uptake and intracellular localization of the AuNPs were demonstrated in HeLa cells. We show that the therapeutic efficacy of the nanohybrid drug was strongly enhanced by the active targeting by the nanoscale delivery system to HeLa cells with a significant decrease of the half-maximal effective drug concentration, through blockage of HeLa cancer cell cycle. These results provide rationale for further progress of AuNPs-assisted combination chemotherapy using two drugs at optimized effective concentrations which act via different mechanisms thus decreasing possibilities of development of the cancer drug resistance, reduction of systemic drug toxicity and improvement of outcomes of chemotherapy.
研究领域:
纳米医学
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肿瘤学
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药理学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2016
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IF 3.902
Pan Zhang,Hui Yang,Guojun Wang,Weijun Tong,Changyou Gao
The poly(allylamine hydrochloride)/trisodium citrate aggregates were fabricated and further covalently crosslinked via the coupling reaction of carboxylic sites on trisodium citrate with the amine groups on polyamine , onto which poly- L- lysine and hyaluronic acid were sequentially assembled, forming stable microspheres. The pH sensitive dye and pH insensitive dye were further labeled to enable the microspheres with pH sensing property. Moreover, these microspheres could be specifically targeted to HeLa tumor cells, since hyaluronic acid can specifically recognize and bind to CD44, a receptor overexpressed on many tumor cells. Quantitative pH measurement by confocal laser scanning microscopy demonstrated that the microspheres were internalized into HeLa cells, and accumulated in acidic compartments. By contrast, only a few microspheres were adhered on the NIH 3T3 cells surface. The microspheres with combined pH sensing property and targeting ability can enhance the insight understanding of the targeted drug vehicles trafficking after cellular internalization.
研究领域:
生物材料学
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肿瘤生物学
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药物递送系统
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL TECHNOLOGY
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2021
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IF 3.247
Xiaoxiang Wang,Tao Lin,Wei Wu,Haisuo Wu,Dongdong Yan
The current investigation reports the synthesis of N-CDs using glucosamine, ascorbic acid, and ethylenediamine precursors by a simple hydrothermal technique. The formation of N-CDs was proved by various characterisation techniques such as X-ray Photoelectron Spectroscopy (XPS), X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), and Fourier-Transform Infrared Spectrophotometer (FT-IR). The optical properties were investigated by fluorescence and UV-vis spectrophotometer. Also, N-CDs showed high selectivity in detecting the MTZ compared to several other analytes. However, the metronidazole serves as an antibiotic against several microbial diseases but also a genotoxic, carcinogenic to the human when used in excessive dosage. The synthesised N-CDs showed high selectivity in detecting the MTZ compared to several other analytes. Besides, the cytotoxicity of the N-CDs was studied to evaluate its toxicity against the HeLa cancer cells. It showed 65.6% cell viability and 34.3% toxicity against the cancerous cells, and similarly 71% of cells viability against H9C2 cells. Thus, the current investigation explores the promising selective sensing of N-CDs against MTZ, along with that, it proved its cytotoxicity against HeLa cancerous cells and non-toxicity against H9C2 cells. The synthesised CDs can be better MTZ sensors and anti-cancer agents on further development at the industrial scale.
研究领域:
材料科学
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分析化学
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毒理学
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DOI
高影响因子
TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY
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2025
·
IF 1.1
Haiyan Peng,Min Wu,Chao Yan,Jian Wan,Hongmao Zhang,Shumao Ding
Carbon nanotubes (CNTs) are ubiquitous in the environment and are widely used in industry and medicine, but their unspecified safety has raised concerns. This study aims to explore the potential hazards of carbon nanotubes. This study exposed different concentrations of single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) to HeLa cells. The results showed that exposure to SWCNTs and MWCNTs resulted in slow growth and decreased survival of HeLa cells. The oxidative stress and autophagy of the cells were intensified, the cell division was abnormal, and the number of S-phase cells was reduced. After 100 μg/ml MWCNTs exposure, the proportion of apoptotic cells increased, and the transcript levels of apoptosis-related genes like Caspase-3 and Bcl-2 were upregulated. In addition, each group’s differentially expressed proteins (DEPs) were mainly related to the regulation of protein catabolic process, glycogen metabolic process, nucleotide synthesis and processing, response to reactive oxygen species, and regulation of cell division and apoptosis. These findings suggest that CNTs induce toxic effects through oxidative stress, exacerbating autophagy and apoptosis, and reducing cell viability. Therefore, the harmful effects of CNTs on organisms should be comprehensively considered in their production and utilization.
研究领域:
毒理学
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细胞生物学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）