RSC Advances
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2021
·Vol. 11
·期 17
·10264-10271
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IF 3.361
Xinyu Wang,Zhuo Li,Jiaojiao Nie,Liangqiang Wu,Weihong Chen,Shaolong Qi,Hai Xu,Jianshi Du,Yaming Shan,Qingbiao Yang
Copper is an essential element in living systems and plays an important role in human physiology; therefore, methods to detect the concentration of copper ions in living organisms are important. Herein, we report a highly water-soluble naphthalimide-based fluorescent probe that can be used for the detection of Cu2+. The probe, BNQ, has high selectivity and sensitivity. The fluorescence intensity of the probe at 520 nm was visible to the naked eye under a UV lamp; upon the gradual addition of Cu2+, there was a colour change from green to nearly colourless. Furthermore, the detection limit of BNQ for Cu2+ was 45.5 nM. The detection mechanism was investigated using a Job's plot and density functional theory (DFT) calculations. In addition, owing to great biocompatibility, we were able to successfully use BNQ to detect Cu2+ in living HeLa cells with low toxicity.
研究领域:
生物化学
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分析化学
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细胞生物学
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DOI
PubMed
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
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2018
·Vol. 107
·期 6
·1786-1791
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IF 3.373
Yujing You,Chenhao Tang,Gang Zhang,Zhiqiang Jiang,Zhongda Lv
Cell growth and release from the hydrogel scaffolds are critical steps in cell culture and cell therapies. Herein a double network hydrogel with one thermosensitive P(AEtMA-Cl-DEAEA) network and the other polyacrylamide network is reported. The diad hydrogel is elastic and maintains its structural integrity under large deformation. BSA is adsorbed onto the hydrogel at body temperature and subsequently released from the hydrogel at lower temperature. Hela cells proliferate on the hydrogel scaffolds at 37°C. When the scaffold is incubated at low temperature (20°C), 32.6% ± 6.4% of its population are detached from the scaffolds. The detached cells remain active in cell culture. This hydrogel with excellent mechanical properties and unique biological function has promising biomedical applications. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1786–1791, 2019.
研究领域:
生物材料学
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细胞生物学
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组织工程学
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DOI
PubMed
高影响因子
RSC Advances
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2016
·Vol. 6
·期 41
·35124-35134
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IF 3.289
Zhirong Bao,Mingyang He,Hong Quan,Dazhen Jiang,Yanhong Zheng,Wenjing Qin,Yunfeng Zhou,Feng Ren,Mingxiong Guo,Changzhong Jiang
Chemoradiotherapy is a well-established treatment paradigm in oncology to consistently improve local tumor control compared to the sole administration of chemotherapy or radiotherapy. Despite its importance, few agents have been identified to further improve the therapeutic ratio and reduce the incidence of complications. Advances in nanomedicine have offered innovative strategies to improve chemoradiotherapy. In the present study, we evaluated the efficacy and safety of FePt nanoparticles (NPs) in chemoradiotherapy using HeLa cells and HEK293T cells. FexPt100−x NPs at different compositions (x = 26, 53 and 77) were synthesized and characterized by means of X-ray diffraction, transmission electron microscopy, and Fourier transform infrared spectroscopy. After ligand exchange, the cytotoxicity of FexPt100−x NPs was evaluated by MTT assay in HEK293T cells while the composition effects of FePt NPs on the cytotoxicity and the potential application of FePt NPs in combination with X-ray radiation at clinically relevant MV energies were investigated in HeLa cells in vitro. Besides, the cellular uptake of NPs was measured indirectly by atomic absorption spectroscopy and transmission electron microscopy. The results indicated that FexPt100−x NPs inhibited the growth of HeLa cells in a concentration- and composition-dependent manner after 24 h incubation, with low cytotoxicity to HEK293T cells at the given concentrations (0–20 μg mL−1). Furthermore, the combination of FePt NPs and radiotherapy resulted in a marked inhibition of HeLa cells, in contrast with that of the individual FePt NPs treated group or the radiation alone group. Moreover, Fe53Pt47 NPs, exhibiting significant cytotoxicity and enhanced radiosensitization effects on HeLa cells without damage to HEK293T cells, might theoretically satisfy the ultimate goal of personalized chemoradiotherapy. Our present work exhibited high therapeutic efficacy of FePt NPs in combination with radiotherapy without apparent cytotoxicity, suggesting the potential of FePt NPs as a promising nanoprobe in improving the outcome of tumor chemoradiotherapy.
研究领域:
肿瘤学
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纳米医学
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放射生物学
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DOI
高影响因子
Asian Pacific Journal of Tropical Medicine
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2015
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IF 1.062
Chong Shi,Guo-Bin Zhang,Shu-Wang Yin
Objective To explore the effect of bortezomib on migration and invasion of cervical carcinoma HeLa cell and specific molecular mechanism. Methods The effect of bortezomib on the viability of HeLa cell was measured by MTT assay. The effect of bortezomib on cell migration and invasion was measured by Transwell assay and invasion experiment respectively. The activation of Akt/mTOR signaling pathway and expression level of MMP2, MMP9 were assayed by western blot. Results MTT assay indicated bortezomib (2.5 μM, 5 μM, 10 μM) could inhibit HeLa cell viability, and the inhibitory rate was highest at 48 h. Transwell assay and invasion experiment results showed that bortezomib inhibited HeLa cell migration and invasion. Western blotting assays presented bortezomib could suppress the phosphorylation of Akt and mTOR, and down-regulate the expression of MMP2 and MMP9. Conclusions These results suggested bortezomib could inhibit migration and invasion in cervical carcinoma HeLa cell, which might be related to Akt/mTOR signal pathway.
研究领域:
肿瘤学
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细胞生物学
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药理学
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2021
·Vol. 413
·期 15
·3945-3953
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IF 4.157
Yang Jin,Chen Weixing,Chen Xinyu,Zhang Xi,Zhou Hongwei,Du Haotian,Wang Mingcheng,Ma Yiting,Jin Xilang
Novel spherical polymer nanoparticles were synthesized by hyperbranched polyethylenimine (hPEI) and 6-hydroxy-2-naphthaldehyde (HNA) via Schiff base reaction (one-pot reaction), which had great advantages in water solubility and green synthesis. Meanwhile, probe PEI-HNA could quickly detect Cu 2+ in the range of 0–60 μM in 30 s with the detection limit of 243 nM. The fluorescence of PEI-HNA-Cu 2+ could be recovered by the addition of S 2− in 50 s with the detection limit of 227 nM. Based on the excellent optical properties, PEI-HNA has been used in the bioimaging of living cells with excellent cell penetrability and low toxicity. More importantly, PEI-HNA has been doped into filter paper, hydrogel, and nanofibrous film to prepare solid-phase sensors, displaying rapid response and excellent sensitivity. Moreover, the low-cost and simple preparation of these sensors offers great potential and possibilities for industrialization, which could help accelerate the development of sensors in environmental and biological fields.
研究领域:
分析化学
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生物医学工程
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材料科学
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DOI
PubMed
高影响因子
Scientific Reports
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2018
·Vol. 8
·期 1
·1-12
·
IF 4.122
Farooq Muhammad U.,Novosad Valentyn,Rozhkova Elena A.,Wali Hussain,Ali Asghar,Fateh Ahmed A.,Neogi Purnima B.,Neogi Arup,Wang Zhiming
Colloidal gold nanoparticles (AuNPs) are of interest as non-toxic carriers for drug delivery owing to their advanced properties, such as extensive surface-to-volume ratio and possibilities for tailoring their charge, hydrophilicity and functionality through surface chemistries. To date, various biocompatible polymers have been used for surface decoration of AuNPs to enhance their stability, payloads capacity and cellular uptake. This study describes a facile one-step method to synthesize stable AuNPs loaded with combination of two anticancer therapeutics, -bleomycin and doxorubicin. Anticancer activities, cytotoxicity, uptake and intracellular localization of the AuNPs were demonstrated in HeLa cells. We show that the therapeutic efficacy of the nanohybrid drug was strongly enhanced by the active targeting by the nanoscale delivery system to HeLa cells with a significant decrease of the half-maximal effective drug concentration, through blockage of HeLa cancer cell cycle. These results provide rationale for further progress of AuNPs-assisted combination chemotherapy using two drugs at optimized effective concentrations which act via different mechanisms thus decreasing possibilities of development of the cancer drug resistance, reduction of systemic drug toxicity and improvement of outcomes of chemotherapy.
研究领域:
纳米医学
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肿瘤学
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药理学
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DOI
PubMed
高影响因子
TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY
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2025
·
IF 1.1
Haiyan Peng,Min Wu,Chao Yan,Jian Wan,Hongmao Zhang,Shumao Ding
Carbon nanotubes (CNTs) are ubiquitous in the environment and are widely used in industry and medicine, but their unspecified safety has raised concerns. This study aims to explore the potential hazards of carbon nanotubes. This study exposed different concentrations of single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) to HeLa cells. The results showed that exposure to SWCNTs and MWCNTs resulted in slow growth and decreased survival of HeLa cells. The oxidative stress and autophagy of the cells were intensified, the cell division was abnormal, and the number of S-phase cells was reduced. After 100 μg/ml MWCNTs exposure, the proportion of apoptotic cells increased, and the transcript levels of apoptosis-related genes like Caspase-3 and Bcl-2 were upregulated. In addition, each group’s differentially expressed proteins (DEPs) were mainly related to the regulation of protein catabolic process, glycogen metabolic process, nucleotide synthesis and processing, response to reactive oxygen species, and regulation of cell division and apoptosis. These findings suggest that CNTs induce toxic effects through oxidative stress, exacerbating autophagy and apoptosis, and reducing cell viability. Therefore, the harmful effects of CNTs on organisms should be comprehensively considered in their production and utilization.
研究领域:
毒理学
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细胞生物学
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DOI
高影响因子
Bioactive Materials
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2017
·
IF 0
Tianmu Hu,Anwar Saeed Ahmed Qahtan,Lei Lei,Zhixin Lei,Dapeng Zhao,Hemin Nie
In order to improve the release pattern of chemotherapy drug and reduce the possibility of drug resistance, poly(ethylene glycol amine) (PEG)-modified alginate microparticles (ALG-PEG MPs) were developed then two different mechanisms were employed to load doxorubicin (Dox): 1) forming Dox/ALG-PEG complex by electrostatic attractions between unsaturated functional groups in Dox and ALG-PEG; 2) forming Dox-ALG-PEG complex through EDC-reaction between the amino and carboxyl groups in Dox and ALG, respectively. Additionally, tuftsin (TFT), a natural immunomodulation peptide, was conjugated to MPs in order to enhance the efficiency of cellular uptake. It was found that the Dox-ALG-PEG-TFT MPs exhibited a significantly slower release of Dox than Dox/ALG-PEG-TFT MPs in neutral medium, suggesting the role of covalent bonding in prolonging Dox retention. Besides, the release of Dox from these MPs was pH-sensitive, and the release rate was observably increased at pH 6.5 compared to the case at pH 7.4. Compared with Dox/ALG-PEG MPs and Dox-ALG-PEG MPs, their counterparts further conjugated with TFT more efficiently inhibited the growth of HeLa cells over a period of 48 h, implying the effectiveness of TFT in enhancing cellular uptake of MPs. Over a period of 48 h, Dox-ALG-PEG-TFT MPs inhibited the growth of HeLa cells less efficiently than Dox/ALG-PEG-TFT MPs but the difference was not significant ( p > 0.05). In consideration of the prolonged and sustained release of Dox, Dox-ALG-PEG-TFT MPs possess the advantages for long-term treatment.
研究领域:
药学
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肿瘤学
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生物材料学
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DOI
PubMed
PHARMACEUTICAL RESEARCH
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2013
·Vol. 31
·期 4
·847-860
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IF 4.742
Tang Lan,Li Ye,Chen Wei-Ying,Zeng Shan,Dong Ling-Na,Peng Xiao-Juan,Jiang Wen,Hu Ming,Liu Zhong-Qiu
Purpose UDP-glucuronosyltransferases (UGTs) are responsible for the formation of glucuronides of polyphenolic flavonoids. This study investigated the UGT1A9-mediated glucuronidation of luteolin and the kinetics of luteolin glucuronide efflux. Method HeLa cells overexpressing UGT1A9 (HeLa-UGT1A9) were used to determine the kinetics of breast cancer resistance protein (BCRP)-mediated transport of luteolin glucuronides. Human UGT isoforms were used to determine glucuronidation rates. Results UGT1A9 was found to catalyze the production of four luteolin glucuronides, including three known monoglucuronides and a novel 3′, 4′-diglucuronide. Ko143, a potent specific inhibitor of BCRP, significantly inhibited efflux of luteolin monoglucuronides from HeLa1A9 cells and increased their intracellular levels in a dose-dependent manner. The formation of luteolin diglucuronide was observed when intracellular concentration of total monoglucuronides went above 0.07 nM. Conclusions Intracellular accumulation of diglucuronide was detected at high monoglucuronide concentrations (>0.07 nM). Diglucuronide production is speculated to be a compensatory pathway for luteolin disposition.
研究领域:
药理学
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分子生物学
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DOI
PubMed
高影响因子
TALANTA
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2018
·
IF 4.244
Yayun Hong,Qiliang Zhan,Chenlu Pu,Qianying Sheng,Hongli Zhao,Minbo Lan
In this work, hollow magnetic macro/mesoporous TiO 2 nanoparticles (denoted as Fe 3 O 4 @H- f TiO 2 ) were synthesized by a facile “hydrothermal etching assisted crystallization” route to improve the phosphopeptide enrichment efficiency. The porous nanostructure of TiO 2 shell and large hollow space endowed the Fe 3 O 4 @H- f TiO 2 with a high surface area (144.71 m 2 g −1 ) and a large pore volume (0.52 cm 3 g −1 ), which could provide more affinity sites for phosphopeptide enrichment. Besides, the large pore size of TiO 2 nanosheets and large hollow space could effectively prevent the “shadow effect”, thereby facilitating the diffusion and release of phosphopeptides. Compared with the hollow magnetic mesoporous TiO 2 with small and deep pores (denoted as Fe 3 O 4 @H-mTiO 2 ) and solid magnetic macro/mesoporous TiO 2 , the Fe 3 O 4 @H- f TiO 2 nanoparticles showed a better selectivity (molar ratio of α -casein/BSA up to 1:10000) and a higher sensitivity (0.2 fmol/μL α -casein) for phosphopeptide enrichment. Furthermore, 1485 unique phosphopeptides derived from 660 phosphoproteins were identified from HeLa cell extracts after enrichment with Fe 3 O 4 @H- f TiO 2 nanoparticles, further demonstrating that the Fe 3 O 4 @H- f TiO 2 nanoparticles had a high-efficiency performance for phosphopeptide enrichment. Taken together, the Fe 3 O 4 @H- f TiO 2 nanoparticles will have unique advantages in phosphoproteomics analysis.
研究领域:
生物化学
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分析化学
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蛋白质组学
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产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
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2014
·
IF 4.086
Xiaobo Wang,Jing Ju,Juan Li,Jiying Li,Qiuhui Qian,Chun Mao,Jian Shen
It is highly desirable to develop simple and rapid method to detect cancer cells. In this case, a simple method for electrochemical detecting cell numbers based cytosensor was investigated. Thioctic acid (TA) monolayer, assembled on a high conductive Au/ITO substrate to stable immobilized of Arg-Gly-Asp-Ser tetrapeptide (RGDS) by chemical bonding, was first used for construction of electrochemical cytosensor. Moreover the cytotoxicity assay also showed no cytotoxicity of TA modified electrode . The proposed cytosensor showed good performance for detection of cancer cells with a broad detection range of 3.0 × 10 2 to 1.0 × 10 7 cells/mL, and a low detection limit of 300 cells/mL. The results were obtained that can be attributed to the combination of the advantages of high conductive and biocompatible property of the modified electrode we suggested. This work demonstrated a simple strategy to prepare an electrochemical cytosensor with the property of low cost, good biocompatible, broad detection range, low detection limit, good repeatability which may have potential large-scale application for improving cancer diagnosis and treatment.
研究领域:
生物医学工程
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癌症诊断
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DOI
高影响因子
COLLOID AND POLYMER SCIENCE
·
2019
·Vol. 297
·期 2
·249-260
·
IF 1.906
Zou Hongyun,Tang Dongyan,Wang Ning,Jia Shuyue,Sun Zhaojie,Yang Xu,Peng Jing
Molybdenum oxide, as one of the important transition metal semiconductor materials with photothermal property, has received increasing attention in the field of catalysis, sensing, energy storage, and clinical biomedicine. To improve its certain limitations such as poor biocompatibility and easy agglomeration during application as biomaterials and promote its performance as photothermal agent in biomedical fields, polyethylene glycol (PEG)–modified molybdenum oxide was synthesized and the synthesis conditions during the hydrothermal process, including the pH values and the amount of PEG added, were adjusted. Owing to the reduction function of PEG, as-obtained PEG-MoO x showed good photostability and colloidal stability, which were beneficial to its application in long-term clinical photothermal therapy. In addition, cell experiments indicated that PEG-MoO x particles exhibited low cytotoxicity and ability of photothermal killing for HeLa cells. With the increase PEG-MoO x particles in solution, the photothermal ablation ability of PEG-MoO x particles for the HeLa cells was enhanced under an 808-nm NIR laser irradiation. Hence, PEG-MoO x particles have great potential in the photothermal treatment of diseases.
研究领域:
生物医学工程
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材料科学
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癌症治疗
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）