ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2021
·Vol. 413
·期 28
·7001-7007
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IF 4.157
Sang Fuming,Yin Suyao,Pan Jianxin,Zhang Zhizhou
A novelty aptasensor for ultrasensitive detection of Hg 2+ is developed, exploiting the combination of plasmonic properties of gold nanoparticles (AuNPs) and exonuclease III (Exo III)-assisted target recycling for signal amplification. In the presence of Hg 2+ , a DNA duplex can be formed due to the strong coordination of Hg 2+ and T bases of single-stranded DNA (ssDNA) probe. Exo III digests the DNA duplex from the 3′ to 5′ direction, resulting in the releasing of Hg 2+ . Then, the released Hg 2+ binds with another ssDNA probe through T-Hg 2+ -T coordination. After Exo III-assisted Hg 2+ cycles, numerous ssDNA probes are exhausted, which promotes poly(diallyldimethylammonium chloride) (PDDA)-induced AuNP aggregation, leading to an obvious color change and aggregation-induced plasmon red shift of AuNPs (from 520 to 610 nm). Therefore, this biosensor is ultrasensitive, which is applicable to the detection of trace level of Hg 2+ with a linear range from 5 pM to 0.6 nM and an ultralow detection limit of 0.2 pM. Furthermore, it enables visual detection of Hg 2+ as low as 50 pM by the naked eye. More importantly, the assay can be applied to the reliable determination of spiked Hg 2+ in sea water samples with good recovery.
研究领域:
生物传感
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环境科学
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纳米技术
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DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 7
·2338-2342
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IF 1.938
Ke Ma,Xing Li,Bin Xu,Wenjing Tian
A fluorescent turn-on approach for the sensitive and selective sensing of Hg2+ based on an aggregation-induced emission fluorescent molecule (DSA-T2) containing 9,10-distyrylanthracene as a fluorophore and thymine as a Hg2+ receptor, was developed. Under optimum conditions, a linear relationship (R2 = 0.9894) was obtained between the fluorescent intensity and the concentration of Hg2+ from 7 × 10−7 mol L−1 to 1 × 10−5 mol L−1. The theoretical detection limit of Hg2+ was evaluated to be 3.4 × 10−7 mol L−1. And the selectivity towards Hg2+ was good compared to other metal ions.
研究领域:
分析化学
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环境科学
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材料科学
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DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2019
·Vol. 412
·期 4
·881-894
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IF 3.286
Xiao Li,Sun Qi,Zhao Qiang,Cheng Xinjian
The detection of heavy metals such as Hg 2+ and Ag + is important and urgent. In this work, – NO 2 /– NH 2 /C=S boron dipyrromethene small molecular derivatives were synthesized at first. Then they were incorporated into polymer chains. The macromolecular fluorescent probes were obtained via Sonogashira reaction using the small molecular probes as building blocks. The as-prepared small-molecule fluorescent probe BO3 exhibits high sensing performance for Hg 2+ . By introducing it into macromolecules, the sensing ability still remains, and even more, the recognition performance is improved. The macromolecular fluorescent probes P1 , P2 , and P3 also have high recognition ability for Ag + with a binding ratio of 2:1 (metal ion to probe ratio). Through the study of the sensing mechanism and the recycling experiments, it is found that the probes responded by the photo-induced electron transfer mechanism and can be recycled and reused. At the same time, BO3 , P2 , and P3 show excellent recognition performance for Hg 2+ in living cells and zebrafish. Living cell imaging experiments indicated that these fluorescent probes had good cell membrane permeability and low cytotoxicity, and could realize bioimaging of Hg 2+ . Therefore, the application value of these fluorescent probes could be enlarged.
Graphical abstract
研究领域:
化学
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生物医学
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材料科学
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DOI
PubMed
高影响因子
ANALYST
·
2018
·Vol. 143
·期 23
·5771-5778
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IF 3.864
Xiaolei Song,Yu Wang,Su Liu,Xue Zhang,Haiwang Wang,Jingfeng Wang,Jiadong Huang
In the present work, a simple, rapid, isothermal, and ultrasensitive homogeneous electrochemical biosensing platform for target Hg2+ detection was developed on the basis of an exonuclease III (Exo III)-aided target recycling amplification strategy. In the assay, a label-free hairpin probe (HP1) was ingeniously designed, containing a protruding DNA fragment at the 3′-termini as the recognition unit for target Hg2+. Also, the DNA fragment in the loop region and 5′-termini (Helper) could be used when a secondary target analog is introduced, but it is caged in the stem region of HP1 when without such a target. The produced secondary target Helper opened the methylene blue (MB)-labeled hairpin probe (HP2) and triggered the Exo III cleavage process, accompanied with the secondary target recycling. This accordingly resulted in the autonomous reduction of the electroactive material MB on the electrode, inducing a distinct decrease in the electrochemical signal. The current developed homogeneous strategy provides a means for the ultrasensitive electrochemical detection of Hg2+ down to the 227 pM level, with high selectivity. It could be further used as a general autocatalytic and homogeneous strategy toward the detection of a wide spectrum of analytes and may be associated with more analytical techniques.
研究领域:
分析化学
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环境科学
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生物化学
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Tingting Liu,Yuhao Li,JingJing Gu,Lei Zhang,Feiyue Qian,BinRong Li,Xuedong Wang
Modulation of the nanozyme's catalytic activity is crucial for its real applications in detecting target analytes. Herein, we fabricated the nanocomposite (NSC/Co 6 Ni 3 S 8 ) of N, S co-doped carbon and Co 6 Ni 3 S 8 by a facile sol-gel approach. Compared to NSC/Ni 9 S 8 , NSC/Co 6 Ni 3 S 8 with bimetallic active sites displayed better enzyme-mimetic activity. This nanocomposite could catalyze O 2 to form ·O 2 − and oxidize colorless 3, 3′, 5, 5′-tetramethylbenzidine (TMB) into blue oxTMB. The other two free radicals (h + and ·OH) played minor roles during the catalytic reaction. Hg 2+ could integrate with S 2− to form HgS and the surface charges of O 2 were transferred to Hg 2+ to promote O 2 adsorption. DFT theoretical calculations highlight that the main reasons for the enhancing effect of Hg 2+ on color development results from electron transfer and increased adsorption energy of O 2 molecules onto the surface of NSC/Co 6 Ni 3 S 8 . By employing the oxidase-like activity of NSC/Co 6 Ni 3 S 8 and Hg 2+ -triggered promoting effect, a colorimetric sensing platform was established for Hg 2+ assay with a linear range of 10–200 μg/L and detection limit of 3 μg/L. Through integration with a smartphone-based APP “Thing Identify” software, a visual colorimetric assay for Hg 2+ was constructed with a detection limit of 5 μg/L. Compared to the data detected by the mercury vapor meter, the relative recoveries of 92.4–108.1% evidenced the higher accuracy of this smartphone-based visual detection. Overall, the NSC/Co 6 Ni 3 S 8 -based colorimetric assay is convenient, rapid, and visual, and can be applied for routine monitoring of Hg 2+ in real-world waters under outdoor conditions.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2017
·
IF 5.401
Zhi-Qiang Zhou,Ren Yan,Jie Zhao,Li-Yun Yang,Ji-Lei Chen,Yan-Jun Hu,Feng-Lei Jiang,Yi Liu
The pollution of Hg 2+ is one of the most serious problems to human health due to their high toxicity, mobility, difficulty in degradation and ability of accumulation in ecological systems. Thus, it is of great importance to develop highly selective and sensitive probe for detecting Hg 2+ in aquatic ecosystems. Here we propose a new turn-on strategy for specific recognition and detection of Hg 2+ . Mn-doped ZnSe quantum dots (QDs) with small size (2.1 nm) were prepared and adopted as probe, the small size of doped QDs makes the replacement of Mn 2+ by Hg 2+ accessible, which eliminates the fluorescence quenching effect of superficial Mn 2+ on Mn-doped ZnSe QDs. Different from traditional semiconductor nanocrystals based turn-off probe for detecting Hg 2+ , we realize a turn-on strategy for the selective detection of Hg 2+ by Hg 2+ -Mn 2+ replacement using semiconductor nanocrystals (Mn-doped ZnSe QDs). This turn-on mechanism was verified by fluorescence spectra, ICP-AES and XPS. In the absence of Hg 2+ , Mn-doped ZnSe QDs possessed low fluorescence intensity. With the addition of Hg 2+ , the fluorescence intensity around 600 nm increased dramatically. Our Mn-doped ZnSe QDs exhibits excellent selectivity and sensitivity for Hg 2+ . The limit of detection is 7.0 nM, which is lower than the mercury toxic level defined by the U.S. Environmental Protection Agency (10 nM). The result of Hg 2+ detection in real samples indicates the feasibility and sensitivity of our probe for application in environmental samples.
研究领域:
环境科学
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分析化学
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DOI
高影响因子
RSC Advances
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2024
·Vol. 14
·期 20
·13808-13816
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IF 3.9
Min Yang,Jian Wang,Xuan Xue,Hechun Jiang
It is imperative to develop a rapid detection method for Hg2+ due to its harm to human health and the ecological environment. In this research, CuO/Au@Cu3(BTC)2 was synthesized through reducing HAuCl4 by CuxO@Cu3(BTC)2, which was obtained by reducing Cu3(BTC)2 with hydrazine hydrate. The oxidase-mimic activity of CuO/Au@Cu3(BTC)2 can be enhanced by Hg2+ through forming a Au–Hg alloy. Therefore, a colorimetric method was designed for Hg2+ detection with a linear relationship in the 0.05–25 μM range and a limit of detection of 9.7 nM. This strategy exhibited a strong selectivity to Hg2+ and was applied in a real water sample with reliable recoveries. This work provides a possibility for the rapid detection of Hg2+.
研究领域:
环境科学
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分析化学
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DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
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2024
·
IF 2.6
Zhao Weiqin,Huang Chun,Guo Xiyu,Zhu Youyu,Li Yuangang,Duan Yingfeng,Gao Jie
Mercury ions (Hg 2+ ) can cause damage to human health, and thus, the study of the detection of Hg 2+ is extraordinarily important in daily life. This work reported a fluorescence biosensor for the detection of Hg 2+ . The key point of this strategy was that the fluorescence of carbon quantum dots made from pomegranate peel (P-CQDs) was quenched by hemin, and restored after G-quadruplex binding with hemin. The presence of Hg 2+ caused thymine (T)-rich DNA fragments to form T-Hg 2+ -T mismatches, and this change allowed the release of G-quadruplex. G-quadruplex could change the fluorescence of hemin/P-CQDs. P-CQDs exhibited excellent properties through characterization analysis, such as transmission electron microscope, X-ray photoelectron spectroscopy and Fourier transform infrared. This proposed fluorescence detection strategy established the linear ranges of Hg 2+ from 1 nM to 50 nM. In conclusion, this simple biosensor had the advantages of strong sensitivity, high selectivity, and low cost for Hg 2+ detection in environmental water samples.
研究领域:
环境科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
·
IF 6.953
Die Wang,Luminita Marin,Xinjian Cheng
The detection of heavy metals, such as Hg 2+ and Fe 3+ , is of great significance. In this work, fluorescent small-molecule BODIPY (BY-3) bearing C C group was synthesized firstly. And then, the chitosan-based polymer sensor CY-1 was synthesized through the spontaneous NH 2 /C≡C click reaction. The synthesized CY-1 can effectively bind and recognize Hg 2+ /Hg + by the –C=N groups formed in the click reaction. Moreover, the macromolecular sensors CS-1 and CS-2 were synthesized by incorporating another recognition sites to CY-1. These synthesized macromolecular sensors can not only recognize Hg 2+ /Hg + , but also effectively recognize Fe 3+ /Fe 2+ . All of them exhibited significant quenching effect, visible to the naked eye under UV irradiation. The detection limit of CY-1 for Hg 2+ was 1.51 × 10 −6 mol/L, and the detection limit of CS-2 for Fe 3+ was 2.30 × 10 −6 mol/L. The BODIPY-chitosan sensors synthesized in this work have the functions of removing heavy metal ions besides the identifying ability. The maximum adsorption capacity of 1 g chitosan to Hg 2+ was 108 mg as the best one. This article provides a new method to prepare macromolecular sensors for the detection and removal of heavy metal ions. As a useful natural polymer, chitosan's application scope was enlarged.
研究领域:
化学传感器
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环境科学
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材料科学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2023
·
IF 8.4
Jiaojiao Yang,Lijun Feng,Jing Liu,Sheng Li,Na Li,Xinfeng Zhang
Mercury (Hg) is an important environmental pollutant because of its high toxicity. With the rapid industrialization, on-site monitoring Hg 2+ contamination becomes increasingly important. In this work, a facile colorimetric sensing system based on DNA-mediated charge neutralization of AuNPs as well as smartphone data acquisition was developed for on-site monitoring of Hg 2+ . The designed both-end block dsDNA system (probe DNA and block DNA) can interact with the replacement DNA and formed T-Hg 2+ -T mismatched dsDNA in the presence of Hg 2+ , thus releasing the long block DNA as well as preventing the ThT-induced neutralization of AuNPs. The color change for the AuNPs neutralization can be pictured and analyzed by a portable smartphone. This on-site Hg 2+ monitoring system was quite simple and sensitive with a low limit of detection (2.8 nM), lower than the maximum permitted amount of Hg 2+ in drinking water regulated by EPA. The accuracy of the proposed method was verified through analysis of two certified water samples, four real water samples and seven cosmetic samples, and the results agreed well with the certified values or those by atomic fluorescence spectrometry . Overall, this facile and simple sensing system is an appealing tool for on-site monitoring of Hg 2+ in water and cosmetic samples.
研究领域:
环境科学
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分析化学
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生物传感
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2020
·
IF 3.594
Baoqin Hao,Xiangfeng Bu,Jiawei Wu,Yanru Ding,Lixia Zhang,Bing Zhao,Yuan Tian
We conducted a related study on the determination of Hg 2+ in water by surface-enhanced Raman scattering (SERS). It is based on acriflavine as a signal probe with the assistance of aggregation agent MgSO 4 for the SERS investigation. Moreover, the density functional theory (DFT) calculations of acriflavine were performed for interpreting the assignments of the vibrational normal modes. The interaction of acriflavine with AgNPs was inhibited because the citrate reduced Hg 2+ to Hg and formed Ag amalgam on the AgNPs surface. The formation of Ag-Hg alloys reduced the SERS intensity of acriflavine. This sensing system displayed excellent sensitivity and a detection limit as low as 1.4 mol L − 1 . The relationship between the SERS intensity and Hg 2+ concentration was found in the range of 0.1–8.0 × 10 −7 mol L − 1 . This method for detecting Hg 2+ is inexpensive, facile and sensitive.
研究领域:
环境科学
·
分析化学
✓ 已验证
DOI
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Xiang Long,Jing Hu,Hongxiang Liu,Shaogui Wu
Ultraviolet-emitting fluorescent carbon dots (U-CDs) were successfully synthesized via a simple one-step hydrothermal method using benzene-1,3,5-tricarboxylic acid as the carbon source and o-phenylenediamine (OPD) as the nitrogen source. The U-CDs had an average particle size of 6.53 nm and were rich in functional groups such as amino, hydroxyl, carboxyl, and carbonyl moieties. Their fluorescence excitation and emission wavelengths were observed at 325 and 365 nm, respectively, with an impressive photoluminescence quantum yield of approximately 20%. The U-CDs also exhibited excellent water solubility, salt resistance, and photobleaching resistance. By harnessing these unique properties, a bifunctional fluorescent probe was developed to detect Hg2+ concentration and temperature. The probe showed a strong linear response to Hg2+ in the range of 0–42 μmol/L and to temperature between 30 and 60 °C, demonstrating high sensitivity and a low detection limit. These characteristics make the probe ideal for analyzing real samples. This work not only expands the family of carbon dots but also introduces a simple and cost-effective method to synthesize multifunctional U-CDs, advancing their application in temperature and metal ion sensors.
研究领域:
材料科学
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分析化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）