INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
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2025
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IF 3.7
Jiarui Tao,Xiao Wang,Changhong Xia,Xingyuan Wang,Yifan Zhang,Jianan Liu,Juhong Zhang,Jun Wang,Jinghui Xi,Shang Wang
The soil-dwelling grubs of Holotrichia parallela inflict substantial damage to the root systems of key cereal crops, including maize and peanuts, thereby posing a serious threat to agricultural productivity. While the olfactory mechanisms through which above-ground insects utilize odorant receptors (ORs) and odorant co-receptors (Orco) to detect plant volatiles for host localization are well established, the role of OR-mediated chemoreception in the host-seeking behaviors of underground pests remains poorly understood. To address this gap, we systematically compared the volatile compounds released by rhizospheric and aerial plant tissues. Through antennal transcriptome analysis and sequence structure prediction, we identified an odorant receptor with biased olfactory expression— HparOR48 . Tissue expression analysis revealed that HparOR48 is predominantly expressed in the larval antennae across developmental stages. The functional characterization of HparOR48 was performed using the Xenopus laevis oocyte expression system, which demonstrated its broad responsiveness to phthalic acid, a root-emitted acidic volatile. Electroantennogram (EAG) assays further confirmed that phthalic acid elicited significant electrophysiological responses in the larval antennae. Moreover, behavioral assays utilizing a four-arm olfactometer indicated that phthalic acid acts as an attractant for the larvae. Our findings provide clear evidence that H. parallela larvae rely on olfactory recognition of rhizosphere-specific acidic volatiles to guide host-seeking behavior, thereby extending our understanding of chemical interactions between soil-dwelling pests and their host plants.
研究领域:
昆虫学
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植物-土壤互作
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化学生态学
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分子昆虫学
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农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL ECOLOGY
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2018
·Vol. 44
·期 10
·965-974
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IF 2.419
Zhang Hongfei,Li Weizheng,Luo Qianwen,Yang Lei,Gong Dongfeng,Teng Xiaohui,Guo Xianru,Yuan Guohui
The castor bean, Ricinus communis L., is a non-host plant for the large black chafer, Holotrichia parallela Motschulsky (Coleoptera: Scarabaeidae). In laboratory bioassays we found that this plant was no less attractive than the main host plant (peanut, Arachis hypogaea ) and three food plant species: velvetleaf ( Abutilon theophrasti ), the glossy privet ( Ligustrum lucidum ), and the Siberian elm ( Ulmus pumila ). In field trapping experiments a Soxhlet extract of castor bean leaves caught more beetles than the optimal sex lure blend [( R )-(−)-linalool and ( L )-isoleucine methyl ester blended in a ratio of 1:4], compared at equal doses (500 μl), and laboratory bioassays indicated that a castor bean plant could enhance the attractiveness of different blend ratios of sex lures. Olfactometer bioassays showed that males prefer volatiles emitted from different combinations of castor bean plant extracts and a signaling female over a female alone. In the presence of castor bean plants copulation rates of H. parallela were highest among all test environments both in laboratory bioassays (60%) and in field tests (70%). This study, combined with our previous observation of the feeding behavior of H. parallela adults on castor bean leaves, suggests that castor bean plants may provide an attractive but risky mating site for H. parallela beetles. The enhancement of male mate-location and copulation rate in the presence of castor bean plants can balance its paralytic effects on H. parallela after intake of potential toxins contained in its leaves.
研究领域:
昆虫学
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生态学
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化学生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CURRENT BIOLOGY
·
2024
·
IF 8.1
Yinliang Wang,Huanhuan Dong,Yafei Qu,Yuxin Zhou,Jianhui Qin,Kebin Li,Chen Luo,Bingzhong Ren,Yazhong Cao,Shuai Zhang,Jiao Yin,Walter S. Leal
Summary Animals have endogenous clocks that regulate their behavior and physiology. These clocks rely on environmental cues (time givers) that appear approximately every 24 h due to the Earth’s rotation; thus, most insects exhibit a circadian rhythm. One notable exception is the scarab beetle, Holotrichia parallela , a severe agricultural pest in China, Japan, South Korea, and India. Females emerge from the soil every other night, reach the canopy of host plants, evert an abdominal gland, and release a pheromone bouquet comprising l -isoleucine methyl ester (LIME) and l -linalool. To determine whether this circa’bi’dian rhythm affects the olfactory system, we aimed to identify H. parallela sex pheromone receptor(s) and study their expression patterns. We cloned 14 odorant receptors (ORs) and attempted de-orphanizing them in the Xenopus oocyte recording system. HparOR14 gave robust responses to LIME and smaller responses to l -linalool. Structural modeling, tissue expression profile, and RNAi treatment followed by physiological and behavioral studies support that HparOR14 is a sex pheromone receptor—the first of its kind discovered in Coleoptera. Examination of the HparOR14 transcript levels throughout the adult’s life showed that on sexually active days, gene expression was significantly higher in the scotophase than in the photophase. Additionally, the HparOR14 expression profile showed a circabidian rhythm synchronized with the previously identified pattern of sex pheromone emission. 48 h of electroantennogram recordings showed that responses to LIME were abolished on non-calling nights. In contrast, responses to the green leaf volatile (Z)-3-henexyl acetate remained almost constant throughout the recording period.
研究领域:
昆虫学
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分子生物学
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行为生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2020
·
IF 4.192
Xiao Wang,Shang Wang,Jiankun Yi,Yunshuo Li,Jianan Liu,Jun Wang,Jinghui Xi
Insects rely on olfaction to locate their host plants by antennae in complex chemical environments. Odorant receptor (OR) genes are thought to play a crucial role in the process. ORs function together with odorant coreceptors to determine the specificity and sensitivity of olfactory reception. The dark black chafer, Holotrichia parallela Motschulsky (Coleoptera: Scarabaeidae), is a destructive underground pest. To understand the molecular basis of H. parallela olfactory reception, an olfactory-biased expressed odorant receptor HparOR27 and HparOrco (HparOR40) were identified from antennal transcriptome analysis and prediction of the sequence structure. Tissue expression analysis showed that HparOR27 was mainly expressed in adult antennae throughout developmental stages. The functions of HparOR27 were analyzed using the Xenopus laevis oocyte expression system. HparOR27 was broadly responsive to three host plant volatiles, including hexanal, lauric acid, and tetradecane. Electroantennogram tests confirmed that three ligands were electrophysiologically active in antennae of female adults. A Y-tube olfactometer test indicated that hexanal was a repellent for adults of both sexes. Taken together, our data support the identification of odorant receptors and provide a molecular basis for eco-friendly pest control.
研究领域:
昆虫学
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分子生物学
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害虫管理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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