FOOD HYDROCOLLOIDS
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2021
·
IF 9.147
Yu-Xiao Wang,Ting Zhang,Jun-Yi Yin,Xiao-Jun Huang,Jun-Qiao Wang,Jie-Lun Hu,Shao-Ping Nie
A homogeneous β-glucan (JHMP-70) was obtained from Hypsizygus marmoreus by alkali extraction and ethanol precipitation, and had an apparent molecular weight of 394 kDa. Its fine structure was investigated by FT-IR, methylation combined with GC-MS and 1D and 2D NMR analysis. Results indicated JHMP-70 was a branched β-(1 → 6)-glucan substituted at O -3 by the non-reducing β-Glc p and/or β-(1 → 3)-linked β-Glc p side chains at a ratio of 1:1. SEM observation revealed that there were micro-spherical shapes intertwined with randomly distributed fibrous filaments. Rheological measurements showed that its flow behavior was highly dependent on the concentration. Newtonian behavior was evident at low concentration, whereas pseudoplastic behavior was observed at high concentration. Besides, the addition of salt ions (Na + or Ca 2+ ) significantly decreased the apparent viscosity of JHMP-70 solution. The low viscosity of JHMP-70 was probably attributed to its structural features, including main chain structure, branching degree and side chain length. This work can provide a basis theory to expand the application of the polysaccharides from H. marmoreus in food industry, because their lower viscosity and superior fluidity are desired in beverage products to impart stability against phase separation without compromising other sensorial properties.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2025
·
IF 9
Yuan Guo,Yajie Zhang,Dianpeng Zhang,Yu Liu,Qiong Yu,Shuang Song,Caige Lu,Jianping Xu,Shouxian Wang
The rapid accumulation of spent mushroom substrate (SMS) presents a critical environmental challenge globally, but efficient and cost-effective recycling approaches remain limited. Here, to advance valorization of SMS, a proof-of-concept study was conducted in which Hypsizygus marmoreus SMS ( Hm SMS) was recycled in the cultivation of Pleurotus ostreatus . Impacts of the Hm SMS-derived bioactive metabolites were further examined systematically. Results showed that integration of 10 %–30 % Hm SMS shortened the harvest time by 2–6 days, significantly improved fruiting body differentiation and productivity of P. ostreatus . The Hm SMS-derived water-soluble and volatile metabolites significantly enhanced P. ostreatus growth, albeit through different metabolic pathways: the former act by reprogramming of the amino acid-related metabolisms, whereas the latter act by upregulating purine metabolism, nucleotide metabolism, and the biosynthesis of phenylalanine, tyrosine and tryptophan. Chemical profiles of SMS were comprehensively investigated using a combined multi-omics approach. The Water-soluble metabolites within Hm SMS comprised primarily lipids and lipid-like molecules (26.8 %; LC–MS) and organic acids and derivatives (31.9 %; GC–MS). Volatile metabolites in Hm SMS were primarily lipids and lipid-like molecules (33.6 %; GC–MS). Five of the examined water-soluble metabolites (fulvic acid, dl-arabinose, erythritol, l-valine and l-isoleucine) significantly promoted P. ostreatus productivity. These findings highlight the untapped potential of SMS-derived bioactive metabolites and imply their potential high-value valorization in forestry and agricultural practices.
研究领域:
真菌学
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可持续农业
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代谢组学
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废弃物回收利用
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真菌生物技术
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DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 14
·3393
·
IF 4.2
Shaoxiong Zhou,Zheng Xiao,Junzheng Sun,Longxiang Li,Yingying Wei,Mengjie Yang,Yanrong Yang,Junchen Chen,Pufu Lai
Hypsizygus marmoreushas abundant proteins and is a potential source for the development of bioactive peptides. However, currently, the research on the bioactive components ofH. marmoreusmainly focuses on polysaccharides, and there is no relevant research on the preparation of bioactive peptides. In this article, an ultrasound-assisted extraction method was used to extract proteins fromH. marmoreus, and then, four peptides with different molecular weight ranges were prepared through protease hydrolysis and molecular classification. The antioxidant and antibacterial activities were also studied. Under the optimal conditions, the extraction rate ofH. marmoreusproteins was 53.6%. Trypsin exhibited the highest hydrolysis rate ofH. marmoreusproteins. The optimal parameters for enzymatic hydrolysis were a substrate concentration of 3.7%, enzyme addition of 5700 U/g, pH value of 7, extraction temperature of 55 °C, and time of 3.3 h. Under these conditions, the peptide yield was 59.7%. The four types ofH. marmoreuspeptides were prepared by molecular weight grading. Among them, peptides with low molecular weight (<1 kDa) had stronger antioxidant and antibacterial activities. This study provides a theoretical basis for the efficient preparation ofH. marmoreuspeptides and the development of antioxidant and antibacterial peptide products.
研究领域:
食品科学
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生物化学
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药理学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2026
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IF 13.2
Yunhe Qu,Ziyan Sun,Xiushi Jia,Yuanning Wang,Xing Yang,Yunmei Ma,Kevin H. Mayo,Lin Sun,Dongfang Shi,Yifa Zhou
A ∼ 20 kDa heteroglucan composed of glucose (Glc) and glucuronic acid (GlcA) was isolated from Hypsizygus marmoreus . Preliminary analysis through methylation analysis and nuclear magnetic resonance (NMR) showed the backbone consisted of β-1,6-linked d -glucopyranosyl (Glc p ) residues, with branches at O-3 positions containing t-β-D-Glc p residues. The linkages of GlcA was carried out using partial acid and glycosidase hydrolysis. HILIC-FLR/ESI-MS n , methylation and NMR of the partial acid and glycosidase hydrolysis products revealed Glc p A was in the forms of t-β-D-Glc p A and β-1,4-D-Glc p A attached to the C-3 position of part of the Glc in the main chain. Furthermore, the β-glucan exhibited structural heterogeneity, comprising three distinct domains: (a) unsubstituted β-1,6-glucan; (b) β-1,6-heteroglucan substituted with GlcA (1–2 sugar residues); and (c) β-1,6-heteroglucan substituted with Glc (single sugar residue). The proposed heteropolysaccharide structure provides critical insights into the structural diversity of mushroom-derived β-1,6-glucans, thereby facilitating future investigations into their structure-activity relationships.
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DOI
PubMed
高影响因子
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