Nanoscale Research Letters
·
2021
·Vol. 16
·期 1
·1-16
·
IF 4.703
Zhou Lin,Huang Zhi,Yang Shanyi,Wei Jiarui,Xu Yan,Hu Lin,Guo Xinrong,Yuan Limin,Yuan Zexuan,Yang Xiaoping,Tao Xiaojun,Zhang Qiufang
Hydrophilic polyethylene glycol monomethyl ether (mPEG) was grafted onto Icariin (ICA) by succinic anhydride to form a polyethylene glycol-Icariin (mPEG-ICA) polymer. The structure of the polymer was characterized by Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy (NMR). mPEG-ICA nanoparticles loaded with ICA were prepared by physical embedding of ICA by dialysis. The particle size was determined to be (220 ± 13.7) nm, and the ζ potential was (2.30 ± 1.33) mV by dynamic light scattering (DLS). Under a transmission electron microscope (TEM), the nanoparticles were spherical, and the morphology was regular. In the medium with pH 7.4, the drug release rate of mPEG-ICA nanoparticles reached (52.80 ± 1.70)% within 72 h. At pH 6.8, the cumulative drug release of nanoparticles reached (75.66 ± 0.17)% within 48 h. Treatment of the nanoparticles with LPS-treated H9c2 cells maintained cell viability, reduced LDH release and exerted antiapoptotic effects. Moreover, ICA-loaded mPEG-ICA nanoparticles significantly decreased the mRNA expression of the myocardial inflammatory cytokines TNF-α, IL-1β and IL-6M. In conclusion, ICA-loaded mPEG-ICA nanoparticles protected against LPS-induced H9c2 cell injury.
研究领域:
药学
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纳米技术
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细胞生物学
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炎症研究
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Zhanpeng Tian,Shuangyi Li,Shumin Zou,Heping Li,Zhiming Zou,Xiuqing Zhang,Qun Tang
A new dual-functional Fe-ICA complex nanocrystal featuring intrinsic antioxidant and colorimetric ammonia-sensitive functions was designed and then combined with cellulose nanocrystal (CNC) to synergistically fortify potato starch (PS) matrix for developing advanced smart active packaging films. The influence of different Fe-ICA: CNC weight ratios on the structural, physical performances, and functional features of PS film was fully studied. Results revealed that the optimized film (PS/Fe-ICA 2 /CNC 4 ) demonstrated significantly improved mechanical strength (8.26 MPa), ductility (27.11 %), hydrophobicity (94.6°), and water/oxygen barrier performances, due to the synergistic effect of well-dispersed Fe-ICA/CNC. PS/Fe-ICA/CNC film was also endowed with potent UV-screening, antioxidant efficiency, colorimetric ammonia-sensitivity, long-term color stability, and biocompatibility. Besides, the PS/Fe-ICA/CNC film presented clear color alteration in response to shrimp spoilage, affirming its applicability for visually monitoring shrimp freshness. This work offered a new ammonia-sensitive smart packaging strategy by combining functional Fe-based complex and CNC through synergistic effects for freshness monitoring.
研究领域:
食品科学
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生物材料
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功能包装
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食品安全
·
纳米复合材料
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分子传感
·
可持续材料
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产品关联
DOI
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2019
·
IF 2.398
Zijian Chen,Kai Zhou,Wuzu Ha,Peihong Chen,Huijun Fu,Yudong Shen,Yuanming Sun,Zhenlin Xu
Melatonin (MT) is a hormone secreted by the pineal gland which regulates sleep rhythm and is widely used in health foods. However, MT is sometimes added illegally and excessively in certain health foods, which might cause undesirable side-effects. In this work, an easy-to-use immunochromatographic assay (ICA) based on mouse monoclonal antibody (mAb) for rapid detection of MT was developed for the purpose of safety. Under the optimal conditions, the developed ICA exhibited a visual limit of detection (VLOD) of 10,000 ng/mL and a LOD of 50 ng/mL (strip reader) with the linear range from 50 ng/mL to 5000 ng/mL. Good correlation was obtained between the results of ICA and standard liquid chromatography (LC) in matrix test and blind samples test. The developed ICA can be applied for rapid detection of samples within 30 min, and is potentially an excellent tool for screening of MT in health foods.
研究领域:
分析化学
·
食品科学
·
生物技术
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Xi Mei,Gan Zhang,Tingting Liu,Hong Hu,Xiaocui Lai,Wenyao Chen,Yumeng Wang,Juan Peng,Weihua Lai
Immunochromatographic assay (ICA) has attracted widespread attention owing to its advantages of economy, simplicity, and rapidity. However, the synthesis of immunoprobes is still limited by complicated design ideas and multistep operations from preparing nanoparticles to conjugating monoclonal antibodies (mAb) onto nanoparticles. Inspired by the biomineralization of zeolitic imidazolate framework-8 (ZIF-8), we proposed a strategy for the rapid synthesis of an integrated immunoprobe (ZIF-8@QDs-mAb), achieving a one-step integration with strong fluorescent signal output capability and specific recognition ability. In addition, different fluorescent colors of ZIF-8@QDs-mAb were generated by doping red and green quantum dots (QDs) in various ratios. With a smart detection platform, the developed ZIF-8@QDs-mAb-based multiplex ICA (ZIF-8@QDs-mAb-mICA) achieved the on-site quantitative detection of enrofloxacin , sulfamethazine , and kanamycin in milk within 15 min, with the limit of detection (LOD) of 0.052, 0.186 and 0.216 ng mL −1 , which were 5.69, 2.20 and 4.40 times higher than that of gold nanoparticles-based mICA, respectively. The quantitative detection of alpha-fetoprotein and human chorionic gonadotropin was also achieved with LOD of 0.516 ng mL −1 and 0.225 mIU mL −1 , respectively, which verified the universality of the strategy. This work provides a novel idea for the design of an efficient integrated immunoprobe and has broad application prospects in ICA.
研究领域:
分析化学
·
纳米技术
·
生物医学工程
·
食品安全
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Xiaoming Li,Jiaqi Hu,De Zhang,Xiubin Zhang,Zhetao Wang,Yufeng Wang,Qiang Chen,Pei Liang
Surface-enhanced Raman spectroscopy (SERS) can quickly identify molecular fingerprints and has been widely used in the field of rapid detection. However, the non-uniformity inherent in SERS substrate signals, coupled with the finite nature of the detection object, significantly hampers the advancement of SERS. Nowadays, the existing mature immunochromatographic assay (ICA) method is usually combined with SERS technology to address the defects of SERS detection. Nevertheless, the porous structure of the strip will also affect the signal uniformity during detection. Obviously, a method using SERS-ICA is needed to effectively solve signal fluctuations, improve detection accuracy, and has certain versatility. This paper introduces an internal standard method combining deep learning to predict and process Raman data. Based on the signal fluctuation of single-antigen SERS-ICA test strip, the double-antigen SERS-ICA test strip was constructed. The full spectrum Raman data of double-antigen SERS-ICA test strip was normalized by the sum of two characteristic peaks of internal standard molecules, and then processed by deep learning algorithm. The Relative Standard Deviation (RSD) of Raman data of bisphenol A was compared before and after internal standard normalization of double-antigen SERS-ICA test strip. The RSD processed by this method was increased by 3.8 times. After normalization, the prediction accuracy of Root Mean Square Error (RMSE) is improved by 2.66 times, and the prediction accuracy of R-square (R 2 ) is increased from 0.961 to 0.994. The results showed that RMSE and R 2 were used to comprehensively predict the collected data of double-antigen SERS-ICA test strip, which could effectively improve the prediction accuracy. The internal standard algorithm can effectively solve the challenges of uneven hot spots and poor signal reproducibility on the test strip to a certain extent, so as to improve the semi-quantitative accuracy.
研究领域:
分析化学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
MICROBIAL PATHOGENESIS
·
2026
·
IF 3.9
Jingyan Wei,Shixin Li,Shuaiqi Ma,Peihang Jiang,Asma Riaz,Fengying Feng,Hongxin Niu,Jian He
Staphylococcus aureus is a major human pathogen mainly involved in chronic biofilm-related infections, especially at epithelial surfaces and wound sites, owing to its ability to form antibiotic-resistant biofilms and release virulence factors. Conventional antibiotics often fail to eradicate established biofilms and may contribute to the emergence of drug resistance, underscoring the urgent need for alternative biofilm-inhibiting and antivirulence strategies. Herein, we synthesized several dicyclic peptides, including cyclo(L-Phe, L-Hyp) and its stereoisomers cyclo(L-Phe, D-Hyp) and cyclo(D-Phe, L-Hyp), to evaluate their ability to inhibit S. aureus biofilm formation. All cyclic dipeptides exhibited a minimum inhibitory concentration (MIC) of 1 mg/mL. Biofilm inhibition was assessed via crystal violet staining and confocal laser scanning microscopy. At 0.5 mg/mL (1/2 MIC), the compounds exhibited superior inhibition of 24 h biofilm formation compared with vancomycin, with cyclo(L-Phe, D-Hyp) showing the most profound inhibitory activity. qRT-PCR revealed that at 0.25 mg/mL (1/4 MIC), cyclo(L-Phe, D-Hyp) significantly downregulated the expression of the Agr -quorum-sensing system ( RNAIII : −75.7%; hla : −61.7%; psm-α : −73.3%), the ica operon ( icaA : −71%; icaD : −76.7%), and sarA (−69.3%) ( P < 0.0001). Notably, cyclo(L-Phe, D-Hyp) showed low cytotoxicity (CC 50 = 5.13 ± 0.27 mg/mL) and negligible hemolysis (<1 %) at twice its MIC, indicating a favorable safety margin for antimicrobial use. These cyclo dipeptides can be formulated for topical delivery to sites such as skin, mucosa, or open wounds, providing a practical approach for localized treatment of biofilm-associated infections. These findings identify cyclo(L-Phe, D-Hyp) as a promising lead for the development of topical anti-infective agents targeting chronic S. aureus biofilm-associated infections.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Xiaozhou Li,Tianyue Yang,Youtao Song,Jinghai Zhu,Deli Wang,Wei Li
Here we describe a surface-enhanced Raman scattering (SERS)-based immunochromatographic assay (ICA) method for the dual detection of two pyrethroid pesticides cypermethrin and esfenvalerate. In the proposed ICA-SERS methods, antibody conjugated gold nanoparticles (AuNPs) were used as the SERS substrate while fluorescence tags labeled on AuNPs were used as the Raman reporters for SERS. Through the immuno-specific combination of antigen and antibody, Raman reporter labeled AuNPs were immobilized on the test line of the ICA strip and facilitated the SERS measurement. And by immobilizing two test lines designed for the detection of two pesticides, a simultaneous dual detection was accomplished. The LODs of the ICA-SERS system was 2.3 × 10 −4 and 2.6 × 10 -5 ng/mL for cypermethrin and esfenvalerate, respectively. The sensitivity was 3 to 4 times higher than ELISA and fluorescent-based ICA methods using the same indirect competitive immunoassay methods. For the three types of samples (tap water, river water, and milk), the recoveries ranged from 94.9% to 112%, with RSD values ranged from 1.3% to 13.5%. The research reveals that this ICA-SERS method possesses high specificity, sensitivity, and reproducibility, and can be reliably used to detect dual pesticides in real samples. An R Shiny-based application 2plex-speclysis was built and used for all the data analyses in this paper. The application was designed to incorporate all the analyses including spectral preprocessing, performance evaluation, and PCA analysis, and make the spectral analysis accessible for people without programming experience.
研究领域:
分析化学
·
环境科学
·
食品科学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 9.8
Qingdan Ye,Wangyu Nan,Li Zheng,Yishu Luo,Qianmin Dong,Yufeng Wang,Pei Liang
The accurate detection of pesticide residues in complex food matrices is significantly hindered by interference from sample components. To address this challenge, we developed a novel surface-enhanced Raman spectroscopy-based immunochromatographic assay (SERS-ICA) for the ultrasensitive and on-site detection of carbofuran. The method employs antibody-conjugated Au@Ag core-shell nanoparticles (Au@Ag NPs) as SERS probes, introducing the Raman reporter molecule 4-mercaptobenzoic acid (4MBA) with stable and easily identifiable characteristic peaks. A competitive immunoassay format was established, leveraging antigen-antibody specificity for selective carbofuran recognition. To maximize detection reliability, we systematically evaluated signal-averaging strategies for SERS-ICA by comparing Raman signals collected at 1, 2, 4, 8, and 16 test-line positions. We identified an eight-point averaging scheme that balances precision (R 2 = 0.9934) and practicality (average recovery = 99.39%). The assay demonstrates remarkable sensitivity with a Raman detection limit of 1 pg/kg (10 −6 mg/kg) and a visual cutoff of 10 μg/kg (10 −5 mg/kg). Validation with three agriculturally relevant vegetables (celery, cowpea, and cucumber) showed excellent performance, with linear correlations (R 2 > 0.99) and near-quantitative recoveries (99.09–99.39%). Blind spike-recovery tests (95.47–101.65%) further confirmed the robustness of the method for real-world applications. This work establishes SERS-ICA as a next-generation platform for field-deployable, matrix-resistant pesticide monitoring with unmatched sensitivity.
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 7
·期 2
·513-520
·
IF 1.938
Yun Xie,Huafang Chang,Kang Zhao,Jianguo Li,Hong Yang,Liyun Mei,Shouming Xu,Anping Deng
In this study, we present a novel immunochromatographic assay (ICA) based on surface-enhanced Raman scattering (SERS) for the sensitive and quantitative determination of clenbuterol in urine samples. The principle of this new ICA is similar to that based on colloidal gold particles, but using AuMBA@Ag–Ab [e.g. the polyclonal antibody of clenbuterol labelled Au@Ag core–shell nanoparticles (NPs) sandwiched with a Raman reporter (4-mercaptobenzoic acid, MBA)] as a probe. The clenbuterol in solution and the clenbuterol–ovalbumin conjugate previously coated on the test line of the ICA strip competed for limited antibody binding sites on the probe. The specific Raman scattering intensity of MBA on the test line was measured for the quantitative detection of clenbuterol. This proposed assay was completed within 15 min. The IC50 and limit of detection (LOD) values of the assay for clenbuterol were found to be 0.02 ng mL−1 and 0.24 pg mL−1, respectively. The relative standard deviations (RSD) of the signal obtained from 10 points along the middle part of the test line were within 4.3–8.7%. There was no cross-reactivity (CR) of the assay with ractopamine or phenylethanolamine A, and only 19% CR with salbutamol. The recoveries of clenbuterol from spiked swine urine samples were in the range of 93.8–112.4% with RSD values in range of 5.2–11.5% (n = 9). The proposed SERS-based ICA was demonstrated to be a rapid, simple and ultrasensitive analytical method for detecting clenbuterol in urine samples and could also be applied for the determination of other target analytes in different matrices.
研究领域:
分析化学
·
食品安全
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 12
·1-9
·
IF 6.232
Su Zixian,Zhao Guangying,Dou Wenchao
Aflatoxin M1 (AFM1) residues in milk pose a major threat to human health, so there is an urgent need for a simple, rapid, and sensitive method for the determination of trace AFM1 in milk. In this study, a competitive immunochromatographic assay (ICA), using visual (PEI/PSS) 4 red silica nanoparticles (SiNPs) as signal amplification probes, was used for the highly sensitive detection of AFM1. The (PEI/PSS) 4 red SiNPs were used to label AFM1 monoclonal antibody (mAb) to prepare ICA for the detection of AFM1. After exploring the optimal conditions of mAb and immunoprobe dosage conditions, the lowest visual detection limit (VDL) of AFM1 in phosphate-buffered saline with Tween 20 (PBST, 10 mM, pH 7.4, containing 1% BSA, 3% sucrose, 1% trehalose, and 0.5% Tween 20) can reach 0.1 pg/mL. The intuitive visually visible value of AFM1 in both PBST and milk was 10 pg/mL. The results showed that the immunochromatographic system based on high chroma color (PEI/PSS) 4 red SiNPs has high sensitivity and broad application prospects for the detection of trace AFM1 residues in milk. The high chroma (PEI/PSS) 4 red SiNPs are expected to be a convenient biomarker for improving the sensitivity of immune chromatography bands.
The schematic diagram shows the detection principle. In this work, in the competitive experiment, (PEI/PSS) 4 red SiNPs were selected as visual labeling materials, and the specific antibody combined with the labeled material was selected as an immune probe. The AFM1-BSA antigen coupled with the macromolecular BSA was fixed on the T line of the nitrocellulose (NC) membrane. The AFM1 in sample solution competes with AFM1-BSA for the specific binding site of immune probe. The detection sensitivity of this method for AFM1 is obtained by judging the change of the red signal intensity produced by the positive sample, compared with the color at the T line of the negative sample.
研究领域:
食品安全
·
分析化学
·
纳米技术
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Mingxin Li,Hong Yang,Shuqun Li,Chunwei Liu,Kang Zhao,Jianguo Li,Danni Jiang,Lulu Sun,Hong Wang,Anping Deng
3-Amino-5-methylmorpholino-2-oxazolidinone (AMOZ) is the metabolite of furaltadone (FTD), one kind of nitrofuran antibiotics which have been banned as veterinary drugs due to the potential harmful effects on human health. AMOZ is stable in tissue matrices, and can be used as an indicator for illegal usage of FTD. In this study, a novel ultrasensitive competitive immunochromatographic assay (ICA) based on surface-enhanced Raman scattering (SERS) for direct detection of AMOZ in tissue and urine samples was developed. Monoclonal antibody (mAb) against AMOZ and Raman reporter 4-mercaptobenzoic acid (MBA) dual labelled colloidal GNPs ( e.g. Ab-Au-MBA) was prepared, characterized and used as an immunoprobe. The specific Raman scattering intensity of MBA on the test line of the ICA strip was measured for quantitative determination of AMOZ. The assay was completed within 15 min. The values of IC 50 and limit of detection (LOD) of the assay for AMOZ were 0.04 ng mL −1 and 0.28 pg mL −1 , respectively. There was no cross-reactivity (CR) of the assay with other three nitrofurans and five compounds. The ICA strips could be used for 7 weeks without loss of activity. The tissue and urine samples were spiked with AMOZ and the recoveries of AMOZ were in range of 87.8–105.9% with the relative standard deviations (RSD) of 1.4–9.0% ( n = 3). The results demonstrated that the proposed SERS-based ICA was able to directly detect AMOZ with high sensitivity, specificity, stability, precision and accuracy, and might be as a powerful method for the analysis of other analytes in different matrices.
研究领域:
分析化学
·
兽医学
·
食品安全检测
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产品关联
DOI
高影响因子
Nanomaterials
·
2024
·Vol. 14
·期 12
·1046
·
IF 4.4
Xiangyang Li,Hean Qian,Jin Tao,Mingshuo Cao,Meng Wang,Wenlei Zhai
Extensive use of pesticides in agricultural production has been causing serious health threats to humans and animals. Among them, phorate is a highly toxic organophosphorus insecticide that has been widely used in planting. Due to its harmful effects on human and animal health, it has been restricted for use in many countries. Analytical methods for the rapid and sensitive detection of phorate residues in agricultural products are urgently needed. In this study, a new method was developed by combining surface-enhanced Raman spectroscopy (SERS) and immunochromatography assay (ICA). Hybrid magnetic Fe3O4@Au@DTNB-Ab nanoprobes were prepared by modifying and growing Au nanoseeds on an Fe3O4core. SERS activity of the nanoprobe was optimized by adjusting the concentration of the Au precursor. A rapid and sensitive assay was established by replacing the traditional colloidal gold-based ICA with hybrid SERS nanoprobes for SERS-ICA. After optimizing parameters including coating antibody concentrations and the composition and pH of the buffer solution, the limit of detection (LOD) for phorate could reach 1 ng/mL, with a linear range of 5~100 ng/mL. This LOD is remarkably lower than the maximum residue limit in vegetables and fruits set by the Chinese government. The feasibility of this method was further examined by conducting a spiking test with celery as the real sample. The result demonstrated that this method could serve as a promising platform for rapid and sensitive detection of phorate in agricultural products.
研究领域:
分析化学
·
环境科学
·
纳米技术
·
农业科学
·
食品安全
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）