Synthesis of a Novel l-Methyl-Methionine–ICG-Der-02 Fluorescent Probe for In Vivo Near Infrared Imaging of Tumors
Purpose A novel near infrared fluorescent probe, l -methyl-methionine (Met)–ICG-Der-02, was synthesized and characterized for in vivo imaging of tumors and early diagnosis of cancers. Method Met was conjugated with ICG-Der-02 dye through the amide bond function by ethyl-3-(3-dimethyllaminopropyl) carbodiimide hydrochloride/ N -hydroxysuccinimide catalysis chemistry. Met–ICG-Der-02 probe uptake was evaluated on PC3, MDA-MB-231, and human embryonic lung fibroblast cell lines. The dynamics of Met–ICG-Der-02 was investigated in athymic nude mice prior to evaluation of the probe targeting capability in prostate and breast cancer models. Results Met–ICG-Der-02 was successfully synthesized. Cell experiments demonstrated excellent cellular uptake of Met–ICG-Der-02 on cancer cell lines without cytotoxicity. Optical imaging showed a distinguishable fluorescence signal in the tumor area at 2 h while maximal tumor-to-normal tissue contrast ratio was at 12 h Met–ICG-Der-02 post-injection. Additionally, dynamic study of the probe indicated intestinal and liver–kidney clearance pathways. Conclusion Met–ICG-Der-02 probe is a promising optical imaging agent for tumor diagnosis, especially in their early stage.
危险品化学品经营许可证（带存储）