SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Fangjun Huo,Jin Kang,Caixia Yin,Jianbin Chao,Yongbin Zhang
A turn on fluorescent probe for cyanide has been rationally constructed based on an intramolecular charge transfer (ICT) mechanism. Upon treatment with cyanide, probe 1 exhibited a strong fluorescence emission based on blocking ICT. The emission showed good linearity with the cyanide concentration in the range of 0–1.8 μM, and the detection limit measured to be as low as 0.05 μM. The NMR experiments confirmed that the ratiometric response of probe 1 to cyanide is due to the electrophilic addition reaction, which results in the inhibition of the ICT process in the probe. Furthermore, the qualitative determination of exogenous cyanide in HepG2 cells demonstrated that the probe can be employed to detect exogenous cyanide in real biological samples.
研究领域:
分析化学
·
生物医学
·
分子生物学
✓ 已验证
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2022
·
IF 6.064
Liu Xina,Zhu Meiqing,Xu Chenyang,Fan Fugang,Chen Panpan,Wang Yi,Li Dongyang
As an inorganic small molecule pollutant, the toxicity and potential carcinogenicity of hydrazine (N2H4) are of increasing concern. In this work, A water-soluble fluorescent probe (OCYB) based on the intramolecular charge transfer (ICT) mechanism for the detection of hydrazine was designed and synthesized. Taking the advantage of 4-bromobutyryl as the recognition group, the high selectivity of OCYB to N2H4 was confirmed by steady-state fluorescence spectroscopy. The limit of detection (LOD) was calculated to be 78 nM in the DMSO-HEPES (pH 7.4) system. The detection mechanism was verified by NMR, HRMS and density functional theory (DFT) calculations. In addition, OCYB exhibits strong anti-interference ability and an “Off-On” fluorescence enhancement effect. Importantly, OCYB can be used to effectively monitor the fluorescence distribution of N2H4 in environmental water samples and organisms.
研究领域:
分析化学
·
环境科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Jianjun Du,Quanyong Gu,Jingyao Chen,Jiangli Fan,Xiaojun Peng
The quantitative recognition of proteins receives great importance in the fields of biology and biomedicine. Though the ratiometric fluorescent probe is a good choice for the quantitative recognition which is less susceptible to the environmental and instrumental interference, very few examples have been reported in the protein identification. In this work, a novel and interesting probe, MY, was developed for the quantitative recognition of serum albumin based on the intramolecular charge transfer (ICT) mechanism. By the structure regulation, the distribution between ICT and twisted ICT state was well controlled. The MY showed a fast, sensitive, and ratiometric response to the serum albumin. Besides, the accurate recognition of serum albumin in practical samples exhibited good recoveries compared with the bromocresol green-based standard method.
研究领域:
生物化学
·
分析化学
·
医学检测
✓ 已验证
产品关联
DOI
高影响因子
TETRAHEDRON
·
2019
·
IF 2.379
Qinghua Hu,Qiuxiang Huang,Yu Mao,Xinlin Liu,Fangrong Tan,Yuyuan Wang,Qiang Yin,Xumeng Wu,Hongqing Wang
In view of the few reports of the near-infrared emissive probe for fluorine ions , we herein designed and synthesized a new easy-to-get colorimetric and near-infrared emissive fluorescent probe ( IS-NR-F ) with a large Stokes shift (>127 nm). Based on specific F − triggered desilylation reaction induced enhanced ICT strategy involving the donor phenolate anion and the acceptor malononitrile , the probe exhibited dual colorimetric and fluorescent turn-on responses, and provided excellent selectivity for fluoride ions. The fluorescent response at 665 nm displayed very good linear relationship in the wide concentration range and deduced a low detection limit of 0.09 ppm. The detection mechanism was confirmed by 1 H NMR, ESI-MS, and TLC calculation. Moreover, probe IS-NR-F has been successfully employed to detect F − in tap water, toothpaste samples, and fluorescent imaging of F − in HeLa cells.
研究领域:
分析化学
·
生物医学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 11
·5606
·
IF 4.9
Rong Wang,Kaiyue Song,Zhaoqin Wei,Yue Sun,Xiaoxia Sun,Yu Hu
The chiral H8-BINOL derivatives R-1and R-2were efficiently synthesized via a Suzuki coupling reaction, and they can be used as novel dialdehyde fluorescent probes for the enantioselective recognition of R/S-2-amino-1-phenylethanol. In addition, R-1is much more effective than R-2. Scanning electron microscope images and X-ray analyses show that R-1can form supramolecular vesicles through the self-assembly effect of the π-π force and strong hydrogen bonding. As determined via analysis, the fluorescence of the probe was significantly enhanced by mixing a small amount of S-2-amino-1-phenylethanol into R-1, with a redshift of 38 nm, whereas no significant fluorescence response was observed in R-2-amino-1-phenylethanol. The enantioselective identification of S-2-amino-1-phenylethanol by the probe R-1was further investigated through nuclear magnetic titration and fluorescence kinetic experiments and DFT calculations. The results showed that this mechanism was not only a simple reactive probe but also realized object recognition through an ICT mechanism. As the intramolecular hydrogen bond activated the carbonyl group on the probe R-1, the carbonyl carbon atom became positively charged. As a strong nucleophile, the amino group of S-2-amino-1-phenylethanol first transferred the amino electrons to a carbonyl carbocation, resulting in a significantly enhanced fluorescence of the probe R-1and a 38 nm redshift. Similarly, S-2-amino-1-phenylethanol alone caused severe damage to the self-assembled vesicle structure of the probe molecule itself due to its spatial structure, which made R-1highly enantioselective towards it.
研究领域:
超分子化学
·
有机化学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yinghong Zhou,Yanting Li,Lixue Yang,Xiaoxia Sun,Yu Hu,Yue Sun
An A-D-A π-conjugated fluorescent probe CBTOP, synthesized via Suzuki coupling and Knoevenagel condensation, serves as a specific tool for chlortetracycline (CTC) analysis. It emits fluorescence over 495–622 nm (blue-to-red). Mechanical grinding triggers its microstructure transformation from columnar to coral-like aggregates, identifiable by fluorescence intensity and color. Under varying water contents, CBTOP self-assembles into nanostructures (sheets, vesicles) via π-π stacking and hydrogen bonding. It specifically recognizes CTC with significant fluorescence quenching and emission blue shift, driven by the ICT mechanism (spectroscopically confirmed). With low cytotoxicity, it enables CTC fluorescent imaging in biological systems, offering a novel specific detection strategy with promising applications.
研究领域:
分析化学
·
生物医学工程
·
分子影像学
·
药物分析
·
纳米生物技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）