SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Jingjie Diao,Jianhua Zu,Gang Han,Yi Xue,Xiaohan Pan,Mingjie Jin,Siyu Liu,Qing Tang
The preparation of cost-effective, environmentally friendly, and highly selective adsorbents is crucial for capturing 90 Sr from radionuclide-contaminated wastewater due to its long half-life and chemical toxicity. In this study, a novel IDA-type chelating resin (PS-IDA) was synthesized by chloroacetylating polystyrene (PS) and introducing iminodiacetic acid (IDA) onto it. Batch adsorption experiments were conducted to investigate the adsorption of Sr 2+ on PS-IDA, including pH, contact time, initial concentration of Sr 2+ , and competing ions. The results indicated that pH 10 was the optimal condition for maximum Sr 2+ ion adsorption. According to isotherm and kinetic data, the adsorption was well-fitted to the Freundlich model and pseudo-second-order kinetic model. Notably, PS-IDA exhibited a high removal rate at pH 10, removing 98.0% and 99.6% of non-radioactive Sr 2+ and 90 Sr, respectively. In simulative groundwater, PS-IDA demonstrated remarkable selectivity for trace levels of 90 Sr ( 90 Sr: Na + : K + : Cs + : Mg 2+ molar ratio = 1: 6.6 × 10 15 : 3.8 × 10 14 : 2.5 × 10 14 : 1.5 × 10 13 ), indicating its potential for selective capture of 90 Sr from contaminated groundwater. Furthermore, PS-IDA resin with a suitable diameter and improved mechanical strength can be used for large-scale remediation of 90 Sr-contaminated wastewater by column chromatography method. Finally, the adsorption mechanism was explained based on experimental data and characterizations which included ion exchange between Sr 2+ in the solution with H + on the resin and then the formation of chelates between adsorbed Sr 2+ and O, N atoms from IDA.
研究领域:
环境科学
·
放射化学
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DOI
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Xiaoxiao Wu,Suleiman A. Haruna,Nazhi Lv,Fangling Jiang,Yu Xia,Qingguo Cai,Qingmin Chen,Xiaomei Chen,Tianhui Jiao,Quansheng Chen
The identification of white tea mainly relies on sensory evaluation and mass spectrometry methods, which have drawbacks such as strong subjectivity and complex analysis. Thus, this study established a novel approach based on colorimetric indicator displacement assay (IDA) sensor to achieve rapid and accurate white tea identification. Cost-effective dyes and phenylboronic acids with different substituents were selected as the indicator and receptor, respectively. A color-signal-responsive IDA sensor array was constructed to enable the rapid and sensitive identification of white tea. Additionally, three supervised pattern recognition algorithms were utilized to process image data. The results demonstrate that combining the IDA sensor with the random forest algorithm accurately differentiates white tea samples by origin and type, achieving a 100% recognition accuracy in both training set (n = 84) and prediction set (n = 56) when ntree = 1000. This work offers an economical and efficient method for the rapid identification of white tea products.
研究领域:
食品科学
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分析化学
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机器学习
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DOI
高影响因子
Legal Medicine
·
2023
·
IF 1.5
Shan Zhang,Shunqin Chen,Faze Zhu,Aimin Wang,Bing Xia,Jie Wang,Jiang Huang,Yubo Liu,Peng Luo
Toxic alkaloids are typically found in herbal medicines and have strong pharmacological effects and a broad therapeutic spectrum. On the other hand, toxic alkaloids exert toxicological activities in vivo ; as such they have a narrow therapeutic window and can induce poisoning due to incorrect dose or misuse. In this view, there is an urgent need to develop a rapid and sensitive assay to detect these toxic alkaloids. This study developed a method for determining five common toxic alkaloids in blood, including brucine, strychnine, aconitine, mesaconitine, and hypaconitine using ultra-high liquid chromatography-tandem quadrupole/linear ion trap mass spectrometry (QTRAP UPLC–MS/MS). The analytes in this investigation were extracted with ether and detected using multiple reaction monitoring (MRM)-information-dependent acquisition (IDA)-enhanced product ion (EPI) scanning modes. SKF 525A served as the internal standard (IS). The approach demonstrated excellent linearity, with a correlation coefficient (R) > 0.9964, and satisfactory sensitivity, with the limit of detection (LOD) of 0.31 ∼ 3.26 ng/mL and a limit of quantification (LOQ) of 1.13 ∼ 11.52 ng/mL. The extraction recovery (ER) was 78.8 ∼ 116.2%, the matrix effect (ME) was −12.3 ∼ 21.2%, and the method accuracy was 0.8 ∼ 12.8%. In addition, the intra-day precision and the inter-day precision (RSD) were 0.7% ∼ 7.4% and 0.4% ∼ 13.5%, respectively. The developed approach is sensitive and efficient, and offer significant application prospect in clinical monitoring and forensic detection of poisoning.
研究领域:
分析化学
·
法医学
·
临床毒理学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2015
·Vol. 3
·期 18
·9789-9798
·
IF 7.443
Rong He,Weiming Li,Dayi Deng,Wensen Chen,He Li,Chaohai Wei,Youwen Tang
In order to develop a highly efficient method for direct removal of lead from acidic wastewater (pH 2.0), we introduce a novel highly ordered Pb2+ imprinted mesoporous silica (PbIMS) SBA-15 combining co-condensation and functionalized iminodiacetic acid (IDA). Fourier transform infrared spectroscopy (FTIR) and energy-dispersive X-ray (EDX) analysis provided the components of PbIMS. The solid-state 13C NMR spectra demonstrated the successful embedment of IDA in the mesoporous silica. Furthermore, X-ray photoelectron spectroscopy (XPS) analysis confirmed that Pb2+ was coordinated by the N atom and carboxyl O atom of IDA. The results of transmission electron microscopy (TEM), small angle X-ray diffraction (XRD) and N2 adsorption–desorption measurement confirmed that the PbIMS preserved a highly ordered hexagonal P6mm mesostructure. The Brunauer–Emmett–Teller (BET) surface area was 762 m2 g−1. The saturated adsorption capacity of PbIMS toward lead reached up to 103 mg g−1 at pH 2.0, which was greater even than that of the simply modified SBA-15 by IDA at pH 4.5. The values of selectivity coefficients of PbIMS for six competing metal ions ranged from 67.3 to 12.7. The adsorption of Pb2+ reached equilibrium within 7 min. The adsorption efficiency of PbIMS was above 93% after six extraction–stripping cycles. The investigation conducted with real samples of strongly acidic wastewater demonstrated the ability of PbIMS to efficiently remove lead below the current China regulatory standard.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
Materials Research Express
·
2022
·Vol. 9
·期 4
·045304
·
IF 2.025
Xiangyu Wang,Jing Zhou,Rui Meng,Yu Zhao,Weihong Guo
This paper investigates the modification of dopamine (IDA) and uses it as a crosslinking agent to enhance the mechanical strength and hydrophobicity of room-temperature-vulcanized (RTV) silicone rubber. IDA was successfully synthesized with dopamine and 3-isocyanatopropyltriethoxysilane. The structure of IDA was verified by Fourier transform infrared spectroscopy and 1H nuclear magnetic resonance spectroscopy. IDA was introduced into the hydroxyl-terminated polydimethylsiloxane (PDMS) system to prepare RTV silicone rubber, and dibutyltin dilaurate (DBTDL) was used as the catalyst. A combination of the scanning electron microscope, thermal gravimetric analysis, universal testing machine, and contact angle was used to analyze the morphology and properties of RTV silicone rubber. The results demonstrated that the addition of IDA significantly enhanced the thermal stability and mechanical strength of RTV silicone rubber. The mechanical strength of the novel RTV silicone rubber increased by 221%. Moreover, the IDA modified RTV silicone rubber had better hydrophobicity as compared to the PDMS polymer prepared with the traditional crosslinking agent.
研究领域:
材料科学
·
有机化学
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产品关联
DOI
高影响因子
ZEITSCHRIFT FUR ANORGANISCHE UND ALLGEMEINE CHEMIE
·
2024
·
IF 1.1
Zhiwei Zhang,Yilin Ma,Encheng Liu,Junjie Cheng,Fengfeng Chen,Bin Ling,Ningyu Di,Junkuo Gao
Curing agents for epoxy resin (EP) are one of the most important branches in the polymer materials field due to their enormous potential in many fields, but constructing them in a well-controlled curing temperature remains challenging. Herein, a novel curing agent is constructed by the zirconium metal-organic framework (UiO-66) and [P 4442 ] 2 [IDA] through an impregnation method. [P 4442 ] 2 [IDA]@UiO-66 is utilized as a functional nanofiller and curing agent to construct the [P 4442 ] 2 [IDA]@UiO-66/EP nanocomposites. The chemical structures of [P 4442 ] 2 [IDA]@UiO-66 were investigated by XRD, SEM, DSC, and TG characterizations. The various [P 4442 ] 2 [IDA]@UiO-66 with different loading amounts of [P 4442 ] 2 [IDA] were used to investigate the curing behaviors of the EP-51. The DSC results confirm that the curing temperature of the [P 4442 ] 2 [IDA]@UiO-66/EP is higher than that of [P 4442 ] 2 [IDA]/EP. In addition, the kinetic parameters and the activation energy of the curing reaction were acquired according to the fitting of Kissinger equation and Ozawa equation. The drying time of [P 4442 ] 2 [IDA]@UiO-66/EP system at 40 °C was enhanced 17 times compare to that of [P 4442 ] 2 [IDA]/EP system. This study opens new avenues for the rational design of EP nanocomposites by employing ionic liquids@MOFs composite materials as curing agent for wide engineering applications.
研究领域:
材料科学
·
化学工程
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Huiyan Jia,Wenxuan Yuan,Zhengyu Ren,Jingming Ning,Yong-Quan Xu,Yujie Wang,Wei-Wei Deng
Herein, a new indicator-displacement array (IDA) sensor was developed for the quality evaluation of black tea fermentation. On the principle of the reversible covalent binding of phenylboronic acid and catechol, phenylboronic acids were selected as acceptors for targeted binding to polyphenols. Pyrocatechol violet and alizarin red S were used as indicators of the reaction. The IDA sensors have sensitive differential responses to fermented tea samples, achieving an assessment of the fermentation degree with accuracies of 80.39–88.00% by support vector machine (SVM). In addition, the key polyphenol components of the fermentation process were accurately predicted by the IDA and SVM regression with ratio of prediction to deviation values of 1.55–1.72, 2.03–2.21, and 2.03–2.08 for total polyphenols, total catechins, and epigallocatechin-3-O-gallate, respectively. In conclusion, the developed IDA sensor is capable of the in-situ quality monitoring of black tea fermentation, with the advantages being cost-effectiveness, sensitivity, and rapidity.
研究领域:
食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2020
·
IF 3.004
Qiuyao Du,Yunfeng Zhang,Jifen Wang,Bingjie Liu
An ultra-performance liquid chromatography tandem triple quadrupole compound linear ion trap mass spectrometry (UPLC-Q-TRAP/MS) method was developed and validated for the detection of hypolipidemic drugs in fingerprints. 13 hypolipidemic drugs were well separated by the gradient elution of 0.01% formic acid in water and methanol at a flow rate of 0.4 mL/min within 11 min. The analytes were detected in positive (ESI + ) and negative (ESI - ) modes and scanned using scheduled multiple reaction monitoring-information dependent acquisition-enhanced product ion (SMRM-IDA-EPI) for best selectivity and sensitivity. The calibration curves showed good linearity in the range of 0.050–50.000 ng/patch with coefficients ( r 2 ) higher than 0.9904 for all analytes. Meantime, the LODs and LLOQs were in ranges of 0.001–0.034 and 0.003–0.050 ng/patch. The accuracies, intra-day and inter-day precision ranged from −13.3 to 0.3%, 1.1–10.4% and 3.7–14.5%, respectively. The recoveries ranged from 79.9 to 114.8%, while the absolute and relative matrix effects were in the range of 83.0–107.2% and 2.2–9.7%. By comparing the non-spiked fingerprints from healthy volunteers with the fingerprints obtained from patients, demonstrated that the method was competent for determination and quantitation of hypolipidemic drugs in fingerprints.
研究领域:
分析化学
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药物分析
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质谱分析
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产品关联
DOI
PubMed
高影响因子
Journal of Orthopaedic Translation
·
2026
·
IF 9.8
Yimin Pi,Haodong Zhu,Jian Xie,Furui Tang,Zhuoya Wang,Li Liu,Yuchun Zhong,Zhidong He,Tian Liu,Jiacheng Zheng,Yuwen Tang,Jun Luo,Cihua Zheng
Inflammatory bowel disease (IBD) is a chronic inflammatory disorder frequently accompanied by extraintestinal complications such as bone loss, yet effective treatment options remain elusive. In this study, we investigated whether hesperetin mitigates colitis-associated bone loss by modulating the “gut–bone axis.” Using a DSS-induced mouse model of chronic colitis, we found that hesperetin significantly reduced intestinal inflammation, restored mucosal barrier integrity, and suppressed serum inflammatory cytokines. Furthermore, hesperetin effectively alleviated DSS-induced bone microstructure damage, promoted the expression of ALP, Runx2, OCN, and inhibited NFATc1 and CTSK. Fecal microbiota transplantation experiments further confirmed that the intestinal microbiota from hesperetin-treated group attenuated intestinal inflammation and bone loss. 16S rRNA sequencing and metabolomics revealed that hesperetin enriched Akkermansia muciniphila ( A. muciniphila ) and increased the tryptophan metabolite 3-indoleacrylic acid (IDA). Supplementation with A. muciniphila or IDA alone simulated the therapeutic benefits of hesperetin. Furthermore, RNA-seq revealed that hesperetin regulated bone metabolism-related gene expression by ECM-receptor interaction and PI3K-AKT signaling, while inhibiting the NOD-like receptor pathway. In conclusion, hesperetin mitigates intestinal inflammation and bone homeostasis imbalance by reshaping the gut microbiota, enriching A. muciniphila, and promoting IDA production, thus providing new insights into potential therapies for IBD-related bone loss. Download: Download high-res image (369KB) Download: Download full-size image
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DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 7
·1056
·
IF 5.561
Xiaoji Cao,Xupin Lin,Congcong Wu,Minghua Zhang,Mingwei Wang
Lotus seed epicarp, a byproduct of lotus, is commonly discarded directly or burned in the cropland, resulting in waste of resources and environmental pollution. In this work, a green ultrasonic-assisted extraction method with ethyl lactate as the extraction solvent was established to extract alkaloids from lotus seed epicarp. The extraction conditions were optimized by response surface methodology. Under the optimal extraction conditions, the extraction of alkaloids from 1 g lotus seed epicarp was accomplished with only 10 mL of extraction solvent within 15 min. Combined with ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry with information-dependent acquisition mode, a total of 42 alkaloids were annotated in the lotus seed epicarp extracts. Among them, 39 alkaloids were reported in lotus seed epicarp for the first time. According to quantitative analysis, the distributions and trends of alkaloids in the lotus seed epicarp were found to be similar to those of lotus leaves. The five growth stages of lotus seed epicarp could be successfully distinguished based on the ten representative alkaloids. This study demonstrates that ultrasonic-assisted extraction with ethyl lactate as extractant solvent was efficient in the extraction of alkaloids from lotus seed epicarp, which is a potential renewable resource of bioactive ingredients.Keywords:lotus seed epicarp;alkaloids;bio-based solvents;pseudo-targeted profile;UPLC-QTOF MS
研究领域:
药学化学
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分析化学
·
环境科学
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DOI
PubMed
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 19
·11524-11534
·
IF 3.119
Kai Zeng,En-Jie Sun,Ze-Wen Liu,Junhui Guo,Chengqing Yuan,Ying Yang,Hao Xie
Magnetic nanoparticles (MNPs) chelating with metal ions can specifically interact with poly-histidine peptides and facilitate immobilization and purification of proteins with poly-histidine tags. Fabrication of MNPs is generally complicated and time consuming. In this paper, we report the preparation of Ni(II) ion chelated MNPs (Ni-MNPs) in two stages for protein immobilization and purification. In the first stage, organic ligands including pentadentate tris (carboxymethyl) ethylenediamine (TED) and tridentate iminodiacetic acid (IDA) and inorganic Fe3O4–SiO2 MNPs were synthesized separately. In the next stage, ligands were grafted to the surface of MNPs and MNPs with a TED or IDA modified surface were acquired, followed by chelating with Ni(II) ions. The Ni(II) ion chelated forms of MNPs (Ni-MNPs) were characterized including morphology, surface charge, structure, size distribution and magnetic response. Taking a his-tagged glycoside hydrolase DspB (Dispersin B) as the protein representative, specific interactions were confirmed between DspB and Ni-MNPs. Purification of his-tagged DspB was achieved with Ni-MNPs that exhibited better performance in terms of purity and activity of DspB than commercial Ni-NTA. Ni-MNPs as enzyme carriers for DspB also exhibited good compatibility and reasonable reusability as well as improved performance in various conditions.
研究领域:
生物化学
·
纳米生物学
·
蛋白质工程
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产品关联
DOI
PubMed
高影响因子
ChemistrySelect
·
2023
·Vol. 8
·期 10
·e202204406
·
IF 2.1
Chibin Pu,Zhongxi Huang,Lihua Huang,Qian Shen,Changmin Yu
Graphical We firstly reported the direct detection and quantification of His-tagged protein based on intramolecular rigidification induced emission in dilute solution, achieved the real-time and fast fluorescent “On” response of His-tagged proteins in protein solution. Our probe could be used in fluorescent labeling of proteins with simple procedure, high quality and low background interference. Histidine tag (His-tag) is a representative epitope tag, which has been widely used in the purification or immobilization of recombinant proteins. In this study, a novel probe CTPE-IDA-Ni 2+ based on intramolecular rigidification induced emission for fluorescence detecting His-tagged proteins is successfully developed and experimentally demonstrated. This detecting system is based on TPE derivative incorporated IDA-Ni 2+ moiety for real-time detection of His-tagged proteins. In the presence of His-tagged proteins, the IDA-Ni 2+ -His-tag complex was formed, which restricted the rotation of two phenyl rings linked with TPE and subsequently resulting in the fluorescence turn on. Results have shown that our probe could directly detect His-tagged proteins with good selectivity and fast response within 10 min. In addition, we have proved that this system could be used in fluorescent labeling of His-tagged protein instead of traditional Western blot assay with simple procedure, high quality and low background interference, as well as without aggregation-caused quenching (ACQ) problems like some organic dyes.
研究领域:
分析化学
·
生物化学
·
分子生物学
·
蛋白质工程
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）