JOURNAL OF RARE EARTHS
·
2022
·
IF 4.632
Haining Song,Haifeng Zhou,Qianqian Zhuang,Zexin Li,Fenglei Sun,Zhenlei Yuan,Youxin Lou,Guangjun Zhou,Yujun Zhao
Oxidizing ClO − and IO 4 − exist widely in environment and are closely related to the health of organisms. Accordingly, fast, sensitive, and direct detection of the two species is significant. Using IFE in UCNPs @PAA and Fe(II)-phenanthroline system, an elegant ratiometric fluorescent nanosensor , without noble metal nanoparticle , was designed for the detection of ClO − and IO 4 − . Fe(II)-phenanthroline complex is used as fluorescent absorber, which can quench green light of UCNPs with gradually varied extent depending on the concentration of Fe(II). The linear zone extends to 800 and 120 μmol/L while the detection limit is 1.30 and 0.58 μmol/L for NaClO and NaIO 4 , respectively. Finally, the nanosensor was successfully applied to detect NaClO and NaIO 4 spiked in milk, spring water, and tap water with good recoveries.
研究领域:
分析化学
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环境科学
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生物医学
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高影响因子
3 Biotech
·
2021
·Vol. 12
·期 1
·1-12
·
IF 2.406
Zhang Dong,Ma Zhi,Chen Hanchi,Ma Weilin,Zhou Jiawei,Wang Qing,Min Chan,Lu Yuele,Chen Xiaolong
A novel strain with antifungal activity against Sclerotinia sclerotiorum was isolated from soil, and identified as Streptomyces sp. ZJUT-IFE-354 using morphological and 16S rDNA sequence analysis. The bioactive metabolite produced by strain ZJUT-IFE-354 was identified and characterized as valinomycin by spectroscopic and chemical methods. The yield of valinomycin was 191.26 mg/L from the culture of Streptomyces sp. ZJUT-IFE-354, which was the highest yield to our knowledge. The in vitro antifungal activity of valinomycin against S. sclerotiorum was investigated as 0.056 ± 0.012 (EC 50 ) and 0.121 ± 0.023 μg/mL (EC 95 ), respectively, which was approximately 10.696- and 30.960-fold more active than that of carbendazim. The results from scanning electron microscopy, cell membrane permeability, and D -sorbitol and ergosterol assay indicated that valinomycin exerted the antifungal activity probably by increasing permeability of fungal cell membrane, leading to mycelial electrolyte leakage, and eventually resulting in the death of S. sclerotiorum . Thus, valinomycin may be a promising antifungal agent to control S. sclerotiorum .
研究领域:
微生物学
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药理学
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植物病理学
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PubMed
高影响因子
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
·
2022
·
IF 3.141
Dong Zhang,Yingling Bao,Zhi Ma,Jiawei Zhou,Hanchi Chen,Yuele Lu,Linjiang Zhu,Xiaolong Chen
Valinomycin is a cyclodepsipeptide antibiotic with a broad spectrum of biological activities, such as antiviral, antitumor, and antifungal activities. However, the low yield of valinomycin often limits its applications in medicine, agriculture, and industry. In our previous report, Streptomyces sp. ZJUT-IFE-354 was identified as a high-yielding strain of valinomycin. In this study, Plackett–Burman design (PBD) and response surface methodology (RSM) were used to optimize components of medium. The optimal medium contained 31 g/L glucose, 22 g/L soybean meal, and 1.6 g/L K2HPO4·3H2O, which could generate 262.47 ± 4.28 mg/L of valinomycin. Then, the culture conditions were optimized by a one-factor-at-a-time (OFAT) approach. The optimal conditions for the strain included a seed age of 24 h, an inoculum size of 8% (v/v), an incubation temperature of 28 °C, an initial pH of 7.2, an elicitor of 0.1% Bacillus cereus feeding at 24 h cultivation, and the feeding of 0.6% L-valine at 36 h cultivation. The final valinomycin production increased to 457.23 ± 9.52 mg/L, which was the highest yield ever reported. It highlights that RSM and OFAT may be efficient methods to enhance valinomycin production by Streptomyces sp. ZJUT-IFE-354.
研究领域:
微生物学
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生物技术
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药学
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DOI
PubMed
高影响因子
Materials Horizons
·
2018
·Vol. 6
·期 2
·375-384
·
IF 13.183
Daoqing Fan,Erkang Wang,Shaojun Dong
DNA computing has shown impressive developments and exhibited amazing power over the past few decades. However, most current DNA logic devices can only produce single-modal output that is monochromic or even invisible, which greatly limits their reliability and practicality, integration capability and computing complexity. Emission-tunable upconversion nanoparticles (UCNPs) are ideal multicolor outputs for DNA computing, however UC luminescent (UCL) DNA logic systems have rarely been reported. Here, for the first time, we report “DNA-Unlocked Inner-Filter-Effect” (DU-IFE) between oxidized 3,3′,5,5′-tetramethylbenzidine (OxTMB) and upconversion nanoparticles (UCNPs) that subtly matches a “lock–key” strategy. Based on the DU-IFE, we construct the first ever UCL DNA logic library. Superior to previous DNA logic systems, this logic library can generate dual-modal (visual and UCL) multicolor label-free outputs, in which RGB (red, green, blue) colorful UCL ones are also visual under harmless NIR-laser. By selectively unlocking the IFE “lock” with different DNA “keys”, diverse basic, advanced and concatenated logic circuits, a logic-controlled molecular RGB-signal-light, triple-parallel-arrays are successfully constructed. All of the above logic operations demonstrate the strengthened reliability and practicality, powerful integration capability and improved computing complexity of this UCL logic library. Moreover, we fabricate an inspiring and useful biosensor with RGB colorful results based on the DU-IFE (a DNA caliper), providing a vivid prototype for potential biological applications.
研究领域:
生物化学
·
分子生物学
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纳米技术
·
生物医学工程
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DOI
高影响因子
Journal of Advanced Research
·
2025
·
IF 13
Mai Luo,Jihai Cai,Sijia Pu,Ting Wang,W. Sijia,Mengwei Wang,Shuangpeng Wang,Songnan Qu,Liang Zou,Peng Li,Jinchao Wei
Introduction Pesticide residues pose a significant threat to human health. Inner filter effect (IFE) stands out for its ability to enable the rapid and cost-effective establishment of fluorescent detection systems. Nevertheless, the practical implementation is often hindered by issues such as low spectral overlap and interference from impurities, which can compromise its effectiveness. Objectives To develop novel AIEE-type CDs and apply them to improve the efficiency of IFE-based strategies. With the aid of AIEE-type CDs, we aim to develop a multi-channel detection platform that provides more reliable fluorescent probes for real sample and imaging applications. Methods Preparation of N, S-CDs was achieved using a novel combination of sources. The activation mechanism of Supra-CDs’ AIEE characteristics was verified. In the methodological experiments, detection performance of Supra-CDs was tested to confirm their improvement in the IFE-based strategy, practical applicability for real sample detection, and imaging capability. Results In this work, p-PA and thiourea were used for the first time to prepare N, S-CDs. After adding water, the fluorescence intensity of Supra-CDs increased by nearly 360 % compared to N, S-CDs. Spectral overlap rates of the target and probe in the controllable IFE-based system improved from 1.80 % to 33.11 %, indicating superior analytical performance in aqueous solutions compared to ethanol. The fluorescent method exhibited a linear range of 0.5–100 μM, with a detection limit of 0.5 μM. Furthermore, a smartphone-based system has been proposed for the colorimetric analysis of methyl paraoxon, offering a more convenient and efficient alternative. Additionally, Supra-CD demonstrated exceptional capabilities in cell imaging, specifically targeting intracellular lipids within HepG2 cells. Conclusion This study unveils a new type of Supra-CDs that exhibit AIEE property. It also introduces a creative fluorescent sensor with tunable IFE. In summary, this research delivers important insights into AIEE-type Supra-CDs and affirms their potential to inspire broader applications.
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高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-9
·
IF 5.479
Wang Weiwei,Wang Yan,Pan Hong,Cheddah Soumia,Yan Chao
A fluorometric method is described for the determination of mucin 1 (MUC1). It is based on the specific binding of MUC1 by anti-MUC1 aptamers and by exploiting the inner filter effect (IFE) exerted by gold nanoparticles (AuNPs) on the blue fluorescence of carbon dots (CDs). When CDs are mixed with AuNPs, their fluorescence is reduced due to an IFE. The IFE efficiency can be modulated by the adsorption and the aggregation state of AuNPs. The latter can be induced by addition of salt, thereby allowing the fluorescence of the CDs to recover. The aptamer is adsorbed on the AuNPs and protects the AuNPs from salt-induced aggregation which is accompanied by a color change from red to blue. If aptamer is added to a mixture of CDs and AuNPs in presence of salt, the aggregation of the AuNPs is inhibited. Thus, the blue fluorescence of the CDs (best measured at excitation/emission wavelengths of 365/448 nm) is reduced. If, however, the aptamers bind MUC1, the aptamers will be released from the surface of the AuNPs. This decreases the salt tolerance of AuNPs and leads to the recovery of the blue fluorescence. The fluorescence intensity increases with the concentration of MUC1. The method has a linear response in the 5.3 to 200 ng·mL −1 MUC1 concentration range and a lower detection limit of 5.3 ng·mL −1 . The method displays excellent selectivity towards MUC1 against other proteins.
Schematic illustration of (I) the influence of IFE of free AuNPs and NaCl aggregated AuNPs and (II) the influence of specific interaction between MUC1 and aptamers on the NaCl-induced aggregation of AuNPs and the IFE efficiency.
研究领域:
分析化学
·
生物传感器
·
纳米技术
·
癌症诊断
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Shasha Feng,Jingjing Lv,Fubin Pei,Xuchu Lv,Yi Wu,Qingli Hao,Yuehua Zhang,Zhaoyang Tong,Wu Lei
A novel method for turn-off sensing 1,1-diamino-2,2-dinitroethylene (FOX-7) in aqueous medium was first proposed based on the inner filter effect (IFE) of FOX-7 on the fluorescence of molybdenum disulfide quantum dots (MoS 2 QDs). Water-soluble MoS 2 QDs as the fluorophore were prepared by the simple hydrothermal method . The morphology, structure, composition and optical properties of the prepared MoS 2 QDs were characterized by Transmission electron microscopy (TEM), Fourier transform infrared (FT-IR), X-ray photoelectron spectroscopy (XPS), UV–vis absorption and photoluminescence spectra. The results showed that the MoS 2 QDs had good water dispersibility and emitted strong photoluminescence with a particle size of 2 nm. Under the optimal experimental conditions, the fluorescence signal of MoS 2 QDs was quenched in the concentrations range of FOX-7 (0.5–100 μM) and the limit of detection (LOD) of the sensor was 0.19 μM. The method had been applied to analyze the real water samples with good selectivity and stability. Moreover, the quenching mechanism was studied systematically by the Fourier transform infrared (FT-IR), UV–vis absorption spectra, fluorescence lifetime , and Stern-Volmer equation, which had been proved to be static quenching. The fluorescence quenching mechanism is mainly IFE and electron transfer.
研究领域:
分析化学
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材料科学
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环境科学
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PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Yuxin He,Hanting Wang,Yuting Yan,Xinhui Jiang,Hecun Zou,Zhengwei Zhang
Gefitinib, a highly significant antitumor drug, is now commonly employed in clinical settings as a first-line treatment for patients with advanced or metastatic non-small cell lung cancer, colon cancer, and breast cancer. Herein, a convenient, rapid, and accurate fluorescence method based on nitrogen-doped carbon dots (NCDs) was designed for ultrasensitive detection of gefitinib. The NCDs were easily synthesized through one-pot hydrothermal process using p -phenylenediamine and D-glutamic acid as the precursors. The sensing strategy relied on the fluorescence of NCDs at 345 nm, which was selectively reduced by gefitinib based on the inner filter effect (IFE). With a broad linear range of 0.025–30 μg/mL and a low limit of detection of 5.5 ng/mL, the probe was successfully applied to the detection of gefitinib in human serum samples, demonstrating strong practicality, affordability, and high accuracy. The proposed sensor is simple in design, fast in detection and cost-effective, and exhibits promising application in drug real-time analysis.
研究领域:
分析化学
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药物分析
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生物医学工程
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Ruichao Chen,Dezhi Wang,Pujian Zhuang,Sisi Chen,Keren Tang,Fang Wu,Dongmei Shi,Jianming Lan,Jinghua Chen
On the basis of inner filter effect (IFE) mechanism, a convenient “off-on” upconversion luminescence (UCL) nanosensor for nitrite (NO 2 − ) and sparfloxacin (SPX) detection was constructed. This nanosensor used upconversion nanoparticles (UCNPs) as luminescent probes and p-aminobenzoic acid (PABA) and N-(1-naphthyl) ethylenediamine hydrochloride (NEDC) as specific recognition elements. Under acidic condition, NO 2 − can react with PABA to generate diazo compounds, which further react with NEDC to generate azo compound 1 (AZO1), and SPX can compete with PABA to bind NO 2 − to generate azo compound 2 (AZO2). Due to the significant overlap between the UCL emission spectrum of UCNPs at 545 nm and the UV–Vis absorption spectrum of AZO1, the resulting IFE effect effectively quenches UCL. Therefore, the attenuation based on UCL intensity can achieve the detection of NO 2 − , while UCL recovery can realize the detection of SPX. The linear range for NO 2 − detection is 0.05–10 μM with a limit of detection (LOD) of 0.0459 μM, while the linear range for SPX detection is 0.05–0.75 μM with a LOD of 0.0193 μM. The proposed nanosensor with “One-stone-two-birds” strategy provides a new approach for fast detection of NO 2 − and SPX.
研究领域:
分析化学
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纳米技术
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环境监测
·
食品安全
·
生物传感
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DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Wanyue Pang,Ruoyu Cao,Shuaijie Guo,Shuo Wang,Xuelian Hu,Yiwei Tang,Xiuying Liu
A versatile fluorescent molecularly imprinted nanosensor (MIPs@O-CDs) for profiling ciprofloxacin (CIP) was innovatively developed using a controllable post-imprinting modification strategy. High-affinity molecularly imprinted polymers (MIPs) as recognition elements granted nanosensor favorable anti-interference. Bright orange-emission carbon dots (O-CDs) as signal transducers demonstrated prominent reverse fluorescence response to CIP due to inner filter effect, ameliorating detection sensitivity and accuracy. Benefiting from inherent advantages, MIPs@O-CDs accomplished selectivity and ultrasensitive ratiometric fluorescence detection of CIP with a linear range of 0.025–6.000 μg mL −1 , a detection line (6.858 ng mL −1 ), a rapid response time (1.5 min) and a distinguishable color transformation (orange-pink-purple). Additionally, a portable optosensing platform has been triumphantly established by integrating MIPs@O-CDs and smartphone-assisted detectors, which achieved precise on-site determination of CIP in milk with satisfactory recoveries (88.185–93.267 %) and a relative standard deviation below 3.960 %. Accordingly, MIPs@O-CDs-based platform demonstrated portability, feasibility, selectivity, and sensitivity, presenting a powerful tool for real-time food safety assessment.
研究领域:
分析化学
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纳米技术
·
生物传感器
·
食品安全
·
荧光光谱学
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材料科学
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PubMed
高影响因子
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
·
2021
·Vol. 38
·期 11
·2100201
·
IF 3.31
Jinxia Xu,Yueyan Zang,Fanyong Yan,Jingru Sun,Yuyang Zhang,Chunhui Yi
Carbon dots (CDs) are emerging photoluminescent materials with excellent optical properties. However, the lack of active sites in primitive CDs has limited their development applications. Herein, functionalized carbon dots (Z-CDs) are successfully prepared by surface modification of CDs with mono (6-amino-6-deoxy) cyclodextrin (β-CD). The introduction of β-CD increases the spatial potential resistance between CDs, which effectively reduces the self-quenching effect. Moreover, the conjugated domains of Z-CDs are expanded, which improves the optical properties with a quantum yield of 48.74%. Z-CDs are able to be used in the sequential detection of morin and Al 3+ , and the fluorescence mechanisms are confirmed to be internal filtration effect and fluorescence resonance energy transfer, respectively. The limits of detection are 0.817 and 0.231 × 10 −6 m . This study not only provides an idea to solve the problem of self-quenching of CDs but also enriches the detection means of flavonoids and ions, which is expected to be applied to biosensing and environmental monitoring.
研究领域:
材料科学
·
分析化学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Jiankang Li,Liuquan Lin,Sen Zhou,Xiaoming Yang
Room-temperature phosphorescence carbon dots (CDs) show the highlighted advantages in chemical sensing and bioimaging owing to its long lifetime, however, acquiring the efficient phosphorescence of carbon dots is still encountering difficulty due to the spin prohibition. Here, we synthesized one type of CDs through one-step hydrothermal method. To be specific, the CDs displayed the blue fluorescence in aqueous, and emitted the bright green phosphorescence when being coated on the filter paper. Importantly, introducing coumarin to the CDs resulted in the distinct quenching of both its fluorescence and phosphorescence. Based on this phenomenon, we successfully established a dual-channel detection of coumarin. The fluorescence detection channel exhibited a linear range of 5–350 μM with a detection limit of 0.196 μM, while the phosphorescence detection channel achieved a linear range of 50–1000 μM with a detection limit of 0.268 μM. The further investigation revealed that IFE and SQE were mainly responsible for the fluorescence quenching of CDs, while the phosphorescence quenching was originated from the increased energy gap between the singlet and triplet states (ΔE ST ) of CDs, thus obstructing the ISC process. Moreover, we employed the CDs as the phosphorescent ink for anti-counterfeiting.
研究领域:
材料科学
·
分析化学
·
生物化学
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高影响因子
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