DESIGNED MONOMERS AND POLYMERS
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2023
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IF 1.6
Hongfu Zhou,Wenjing Xie,Anran Guo,Bin Chen,Sanming Hu,Min Zheng,Houqiang Yu,Hongan Tian,Ling Li
Several vascular embolization materials are commonly used in clinical practice, however, having application defects of varying degrees, such as poor intraoperative imaging and easy recanalization of embolized blood vessels, they are challenging for application during Transcatheter arterial embolization (TAE). Thus, an intraoperative visible vascular embolization material with good embolization effect and biocompatibility can improve transcatheter arterial embolization clinical efficacy to some extent. Our study aimed to synthesize a novel vascular embolization material that can achieve complete embolization of arterial trunks and peripheral vessels, namely poly (N-isopropyl acrylamide)-co-acrylic acid nanogel (NIPAM-co-AA). Iohexol 200 mg/mL was co-assembled with 7 wt% NIPAM-co-AA nanogel to create an intelligent thermosensitive radiopaque nanogel (INCA), which achieves a good intraoperative imaging effect and is convenient for transcatheter arterial bolus injection due to its good fluidity and temperature-sensitive sol-gel phase transition. The normal rabbit kidney embolism model further confirmed that INCA could effectively use Digital subtraction angiography (DSA) to achieve intraoperative imaging, and real-time monitoring of the embolization process could avoid mis-embolization and leakage. Meanwhile, in a 42-day study, INCA demonstrated an excellent embolization effect on the right renal artery of New Zealand white rabbits, with no vascular recanalization and ischemic necrosis and calcification remaining. As a result, this radiopaque thermosensitive nanogel has the potential to be an intelligent thermosensitive medical vascular embolization material, providing dual benefits in TAE intraoperative imaging and long-term postoperative embolization while effectively addressing the shortcomings and challenges of commonly used clinical vascular embolization agents.
研究领域:
医学影像学
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介入放射学
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生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2024
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IF 11
Honglang Liu,Yongqing Wu,Yanxin Li,Xin-an Xie,Pan Li,Bing Du,Lu Li
In this paper, the effects of different concentration (0.05–0.5 M) of metal ions (Na + , K + , Ca 2+ and Al 3+ ) on the gel properties and microstructure of Inca peanut albumin (IPA) were investigated. Results showed that the formation of IPA gel network was mainly in the cooling stage. The hardness, water holding capacity (WHC) and energy storage modulus of the gels increase when the NaCl and KCl concentration are lower than 0.1 M. With the increase of CaCl 2 concentration, the hardness and energy storage modulus of the gels first increased and then decreased, but the WHC did not change significantly. Moreover, 0.1 M AlCl 3 increased the WHC, surface hydrophobicity and Zeta potential of the gels, but the average particle size decreased. The above results indicate that different metal ions have different effects on the properties of IPA gels. Metal ions induced the interconversion of the IPA secondary structure, in which the increase in the content of β-sheet structure might be one of the reasons for the enhanced gel properties. NaCl, KCl and CaCl 2 induced the gels to form a tighter and more homogeneous fine-chain structure, whereas AlCl 3 induced the IPA gel network to form a "sheet-like" structure. In conclusion, the gels' microstructure and properties were improved by the low concentration of NaCl and KCl (<0.1 M), and the gels' distinctive lamellar structure created by 0.1 M Al 3+ was better suited to improving their WHC. These results will provide a valuable basis for the application of IPA gels in food colloids .
研究领域:
食品科学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Microbiology
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2024
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IF 4
Zhou Haibo,Lu Zhaoxin,Liu Xinmei,Bie Xiaomei,Cui Xinping,Wang Zuwei,Sun Xiaojie,Yang Jun
Objectives: The purpose of this study was to determine the structural features and transferability of the multidrug-resistance (MDR) plasmid, and resistance phenotypes for the tested antimicrobials in foodborne Vibrio parahaemolyticus.Methods: Plasmids were isolated from a V. parahaemolyticus strain of seafood origin, then sequenced using the Illumina NovaSeq 6000 and PacBio Sequel II sequencing platforms to obtain the complete genome data. Characterization of the MDR plasmid pVP52-1, including determination of antimicrobial resistance genes (ARGs), plasmid incompatibility groups, and transferability, was carried out.Results: V. parahaemolyticus strain NJIFDCVp52 contained two circular chromosomes and two circular plasmids (pVP52-1 and pVP52-2). Plasmid typing indicated that pVP52-1 belonged to the incompatibility group IncA/C2 and the sequence type pST3. pVP52-1 carried 12 different ARGs, an IS110-composite transposon consisting of aac(6′)-Ib-cr, qnrVC1, aac(6′)-Ib, dfrA14, and the IS26-mphA-IS6100 unit flanked by inverted sequences of IS5075 and IS4321. pVP52-2 carried no ARGs. A plasmid elimination assay showed that only pVP52-1 and its ARGs were lost, the loss of resistance to several antimicrobials, causing a change from the ampicillin-ampicillin/sulbactam-cefazolin-cefoxitin-ceftazidime-cefotaxime-imipenem-trimethoprim/sulfamethoxazole resistance pattern to the ampicillin resistance pattern. In accordance, a conjugation transfer assay showed that only pVP52-1 and its ARGs were horizontally transferred, leading to increased antimicrobial resistance in Escherichia coli strain EC600, causing a change from the ampicillin-nalidixic acid resistance pattern to the ampicillin-ampicillin/sulbactam-cefazolin-cefoxitin-ceftazidime-cefotaxime-imipenem-nalidixic acid-chloramphenicol-tetracycline-trimethoprim/sulfamethoxazole-azithromycin resistance pattern. Further transferability experiments revealed that pVP52-1 could be transferred to other enterobacterial strains of E. coli and Salmonella.Discussion: This study emphasizes the urgent need for continued surveillance of resistance plasmids and changes in antimicrobial resistance profiles among the V. parahaemolyticus population.
研究领域:
微生物学
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分子生物学
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食品安全
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抗菌素耐药性
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）