LANGMUIR
·
2018
·
IF 3.789
Fang Cheng,Chuanlei Zhu,Wei He,Jing Zhao,Jingping Qu
Two types of zwitterionic polymer-modified magnetic nanoparticles (NPs) are fabricated by conjugating pSBMA onto PEI-precoated NPs via either a one-step method (1S NPs) or two-step method (2S NPs). For both methods, divinyl sulfone is used as the linker molecule. Although 1S NPs were capable of resisting both IgG and BSA, 2S NPs exhibited specificity toward IgG adsorption in complex biological fluids, e.g., in a mixture of serums and IgG. The moderate interactions (Kd ≈ 1.2 μM) between IgG and 2S NPs are 3 orders of magnitude lower than IgG binding with protein A (Kd 10 nM). Through complementary characterizations and analyses, we rationalize that the surface developed herein with IgG specificity contains two key components: polyzwitterions with a short chain length and sulfone groups with a high density.
研究领域:
生物材料学
·
免疫学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2013
·
IF 4.612
Hong-Fei Tong,Dong-Qiang Lin,Dong Gao,Xiao-Ming Yuan,Shan-Jing Yao
Hydrophobic charge-induction chromatography (HCIC) is a novel downstream bioprocessing technology for antibody purification and it has several advantages over traditional purification processes. However, its separation selectivity still needs to be improved. In this work, sodium caprylate (NaCA) was used as the selective modifier to improve IgG purification from serum albumin containing feedstock with a typical HCIC resin, MEP HyperCel. The effects of NaCA on the adsorption equilibrium of bovine serum immunoglobulin G (IgG) and bovine serum albumin (BSA), as well as the dynamic binding and displacement behaviors were investigated. The binding and elution behaviors of these two proteins in the column were studied. It was found that adding 50–75 mM NaCA in the liquid phase could effectively reduce the adsorption of BSA on the MEP resin, but the same treatment has little influence on the adsorption of IgG. Moreover, the mechanism of the competitive binding between caprylate and MEP ligands on the surface of BSA is discussed. It was found that by controlling NaCA addition in the loading or washing buffer, the process efficiency of IgG purification from BSA containing feedstock can be improved, and the purity of IgG could reach to over 98%. The results indicated that caprylate could be a promising albumin-selective modifier to improve the separation efficiency of antibodies with the HCIC process.
研究领域:
生物技术
·
蛋白质纯化
·
抗体工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2020
·
IF 2.87
Jiaping Guo,Yameng Wang,Shanshan Niu,Hongping Li,Yongmei Tian,Songcheng Yu,Fei Yu,Yongjun Wu,Li-e Liu
The aim of this study is to establish a new method with high sensitivity, accuracy, and stability for the determination of human IgG and then expand it to analyze severe acute respiratory syndrome corona virus 2 (SARS-CoV-2)-specific IgM and IgG, which is of great significance for the screening and diagnosis of COVID-19. In this study, the magnetic Fe3O4 nanospheres coupled with mouse antihuman IgG (Ab1IgG) were used as an immune capture probe (Fe3O4@Ab1IgG) to capture and separate the target, and rabbit antihuman IgG (Ab2IgG) coupled with highly luminescent quantum dot nanobeads (QBs) as a fluorescence detection probe (QBs@Ab2IgG) was used to realize high sensitivity detection. After the formation of a sandwich immunocomplex, the fluorescence intensity of the precipitate after magnetic separation was measured at the excitation wavelength of 370 nm. Under optimal conditions, a wide linear range varying from 0.005 to 40 ng·mL–1 was obtained for the detection of human IgG with a lower limit of detection at 4 pg·mL–1 (S/N = 3). The recoveries of intra- and interassays were 90.0–101.9 and 96.0–106.6%, respectively, and the relative standard deviations were 6.3–10.2 and 2.6–10.5%, respectively. Furthermore, the proposed method was successfully demonstrated to detect human IgG in serum samples, and the detection results were not statistically different (P > 0.05) from commercial enzyme-linked immunosorbent assay kits. This method is sensitive, fast, and accurate, which could be expanded to detect the specific IgM and IgG antibodies against SARS-CoV-2.
研究领域:
生物化学
·
纳米技术
·
分析化学
·
免疫学
·
病毒学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2016
·Vol. 21
·期 3
·793-801
·
IF 2.327
Dong Sheying,Li Miao,Wei Wenbo,Liu Dan,Huang Tinglin
A substantial outstanding challenge in diagnostics and disease monitoring is the ability to assay rapidly and conveniently for protein biomarkers within complex biological media. Bi 2 Se 3 , as an important topological insulator (TI) material, was synthesized by a solvothermal method and characterized structurally. Subsequently, the composite of Bi 2 Se 3 and ionic liquid ([BMIm]BF 4 IL) was used as a sensing interface to cross-link goat anti-human immunoglobulin G (anti-IgG) via glutaraldehyde (GA) to fabricate an Bi 2 Se 3 /IL/GA/anti-IgG-carbon paste electrode (CPE). The nonspecific binding sites were enclosed with bovine serum albumin (BSA) to develop a label-free IgG immunosensor. The result showed that the proposed label-free IgG immunosensor exhibited high specificity with a detection limit of 0.8 ng mL −1 and linear range from 2 to 300, and 300 to 2200 ng mL −1 . Besides, the immunosensor exhibited high specificity for IgG detection, acceptable reproducibility, and stability. Thus, the strategy reported here paved a simple way to design a sensitive and cost-effective sensing platform for extension to other disease biomarkers.
研究领域:
生物医学工程
·
材料科学
·
诊断学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 28
·3411-3419
·
IF 3.1
Jiajia Wang,Wenhui Dong,Xiaomin Yang,Yanan Li,Baokang Jin
Electrochemical detection of human-IgG via biosensors is vital in clinical diagnostics, owing to their simple equipment, facile operation, high selectivity, economical, short diagnostic time, fast response, and easy miniaturization, but the need to improve sensitivity for protein detection is still a barrier limiting its wider practical applications. A hypersensitized electrochemical biosensor based on steric effects for IgG detection was developed in this work. The results indicate that IgG-modified sig-DNA attached to CdTe quantum dots (CdTe-sig-DNA) limited the ability of CdTe-sig-DNA or CdTe-sig-DNA-IgG conjugate to hybridize through the captured DNA strand (cap-DNA) immobilized on a chitosan/nitrogen-doped carbon nanocomposite (CS/N-C) modified glassy carbon electrode surface (GCE). The concentration of IgG based on CdTe concentration was detected by differential pulse anode stripping voltammetry (DPASV) on the electrode surface. The efficiency for hybridizing CdTe-sig-DNA with cap-DNA was found to be logarithmically inverse to the concentration of IgG attached. A highly sensitive and selective detection of IgG from 5 pM to 50 μM with a relatively low detection limit of 1.7 pM was achieved. Therefore, the steric hindrance effect of IgG limited the quantity of DNA that could be functionalized on CdTe QDs, significantly improving the signal, and providing a practical strategy for the clinical analysis of IgG.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2020
·Vol. 16
·期 26
·2000239
·
IF 11.459
Zhihe Guo,Yingchun Qin,Peizong Chen,Jinliang Hu,Yi Zhou,Xuyang Zhao,Zhiran Liu,Yiyan Fei,Xiaoshun Jiang,Xiang Wu
Whispering gallery mode (WGM) microresonators have been used as optical sensors in fundamental research and practical applications. The majority of WGM sensors are passive resonators that require complex systems, thereby limiting their practicality. Active resonators enable the remote excitation and collection of WGM-modulated fluorescence spectra, without requiring complex systems, and can be used as alternatives to passive microresonators. This paper demonstrates an active microresonator, which is a microdisk laser in a hyperboloid-drum (HD) shape. The HD microdisk lasers are a combination of a rhodamine B-doped photoresist and a silica microdisk. These HD microdisk lasers can be utilized for the detection of label-free biomolecules. The biomolecule concentration can be as low as 1 ag mL −1 , whereas the theoretical detection limit of the biosensor for human IgG in phosphate buffer saline is 9 ag mL −1 (0.06 a M ). Additionally, the biosensors are able to detect biomolecules in an artificial serum, with a theoretical detection limit of 9 ag mL −1 (0.06 a M ). These results are approximately four orders of magnitude more sensitive than those for the typical active WGM biosensors. The proposed HD microdisk laser biosensors show enormous detection potential for biomarkers in protein secretions or body fluids.
研究领域:
生物医学工程
·
光学传感
·
分子诊断
✓ 已验证
DOI
高影响因子
Biosensors-Basel
·
2022
·Vol. 12
·期 3
·141
·
IF 5.743
Yijian Huang,Ying Wang,Gaixia Xu,Xing Rao,Jiaxiong Zhang,Xun Wu,Changrui Liao,Yiping Wang
A compact surface plasmon resonance sensor based on an H-shaped optical fiber is proposed and demonstrated. The H-shaped optical fiber was fabricated experimentally by using hydrofluoric acid to controllably corrode the polarization-maintaining fiber. A satisfactory distance between the outer surface of the fiber and the core can be achieved, and then the surface plasmon resonance effect can be excited by coating a metal film of appropriate thickness on the surface of the fiber. This technology can realize the preparation of multiple samples at one time, compared to the traditional side-polishing technique. The H-shaped optical fiber obtained from corrosion exhibits a high surface quality and short lengths, down to only a few hundred microns. The effects of the proposed H-shaped optical fiber on spectral properties are induced by process parameters, including fiber remaining thickness, coating thickness and fiber length, and were investigated in detail. The prepared sensor was used for the specific detection of human IgG, and the minimum human IgG concentration that the sensor can distinguish is 3.4 μg/mL. Such a compact surface plasmon resonance fiber sensor has the advantages of an easy fabrication, good consistency and low cost, and is expected to be applied in the specific detection of biomarkers.Keywords:surface plasmon resonance;biosensor;polarization-maintaining fiber;chemical corrosion
研究领域:
光学传感
·
生物医学工程
·
光纤技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2024
·
IF 5.8
Jiayi Zhu,Chao Zhao,Binyun Xia,Ning Wang,Xi Chen,Xinyue Jing,Minxuan Chen,Xinrui Xu
Abnormal human immunoglobulin G (IgG) may induce the risk of immune system disorder, infectious diseases, tumors and so on. However, the current detection methods exhibit low sensitivity, which limits their practical application. In this work, an SPR optical fiber sensor (SPR-OFS) with high sensitivity is designed for label-free detection of human IgG. It is fabricated using a heterostructure optical fiber coated with Au film/AuNPs and the Ti3C2Tx MXene biofunctionalized with goat anti-human IgG by polydopamine (PDA). In the experiment, the optimal thickness of the Ti3C2Tx MXene was explored and determined to be about 93 nm by comprehensively considering the refractive index (RI) sensitivity and spectral bandwidth of the SPR sensor. When the largest figure of merit (FOM) is calculated to be 17.8279 RIU−1, its RI sensitivity was ultimately found to be 2804.5 nm per RIU. The SPR-OFS was employed to detect human IgG within the concentration range of 0–30 μg mL−1 and its sensitivity is demonstrated to be 1.7046 nm (μg mL−1)−1. The SPR-OFS was also proved to have excellent linearity, specificity and stability. The proposed sensor offers outstanding performance with simple fabrication, providing a cutting-edge bioanalytical platform with potential applications in clinical diagnosis.
研究领域:
生物传感技术
·
医学诊断
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2018
·
IF 2.746
Sizhu Tian,Jia Jiang,Shuang Zang,Kun Wang,Yong Yu,Xuwen Li,Hanqi Zhang,Aimin Yu,Ziwei Zhang
A novel electron spin resonance (ESR) spectroscopy method for determination of rabbit IgG was presented. Anti-rabbit IgG antibody was bound to Fe 3 O 4 nanoparticles (FeNPs). The FeNPs were used as probe to give ESR signal . After the optimization of the assay, a molar ratio 500:1 of antibody to FeNPs was selected. The sepharose beads were used as solid phase and used to separate the analyte from the sample solution. Two typical immunoassay formats, sandwich and competitive formats, were applied to the determination of IgG. The analytical sensitivities obtained by sandwich and competitive formats were 0.13 μg mL −1 and 0.56 μg mL −1 , respectively. The two formats were applied to the determination of the rabbit IgG in rabbit serum. The diluted sample was directly analyzed and other additional sample treatment was not required. The analytical results of the spiked samples indicated that the recoveries of the analytes were acceptable. Furthermore, the sample preparation time used in this method is shorter than most existing immunoassay methods .
研究领域:
分析化学
·
免疫学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2015
·
IF 2.729
Zhui Tu,Yang Xu,Jinheng Fu,Zhibing Huang,Yao Wang,Bin Liu,Yong Tao
This work aimed to evaluate novel affinity resin used to purify immunoglobulin G (IgG) with a variable domain of the heavy chain of the heavy-chain antibody (VHH) as an affinity ligand. The VHH, isolated from a naïve camelid single-domain phage display library, exhibits not only affinity to the fragment crystallizable (Fc) region of IgG but also high thermal stability . This anti-Fc VHH (AFV) was expressed as a soluble protein in Escherichia coli and purified using a simple heat treatment procedure. The effects of pH and NaCl concentrations on the capacity of AFV resin were also investigated. Results showed a robust property of the AFV resin. It could bind IgGs at various pH conditions (from 6.0 to 9.0) and NaCl concentrations. The static binding capacities of AFV resin ranged from 3.40 ± 0.53 mg/ml to 15.04 ± 0.37 mg/ml measured using rabbit, mouse, and human IgGs. The bound IgGs can be efficiently eluted at pH 5.0, which is conducive to acid-sensitive IgGs and prevents the aggregation of IgGs. After 10 purification cycles or a 7-day period of storage at 37 °C, recovery did not decrease. These findings suggested that VHHs from non-immunized library could also be robust and functional reagent as an affinity purification ligand.
研究领域:
生物技术
·
生物化学
·
分子生物学
·
蛋白质工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2022
·
IF 5.76
Axin Liang,Shanshan Tang,Miao Liu,Yue Yi,Bingteng Xie,Huipeng Hou,Aiqin Luo
Human IgG is one of the most important immunoglobulins in the human body. The present study described the fabrication of four kinds of layer-by-layer structures of copper metal-organic frameworks (Cu-MOFs) on the working electrode by electrodeposition, which were then applied as an electrochemical sensor for the sensitive determination of IgG in serum. First, MOFs synthesized using different deposition potentials are expected to have varied morphology and properties. Herein, four copper MOFs (Cu-MOFs) were electrosynthesized by a simple and direct reduction approach. The as-synthesized Cu-MOFs exhibit varied morphology and electrocatalytic behavior. Then, IgG was employed as a template in the electropolymerization of pyrrole-imprinted films on the surface of glassy carbon electrodes. Finally, the template protein was removed to form a molecularly imprinted film with the capability to qualitatively and quantitatively signaling of IgG. Under optimized conditions, the sensor for IgG exhibits a wide detection range of 0.01–10 ng mL −1 with a limit of detection (LOD) of 3 pg mL −1 (S/N = 3). Besides, other parameters including the selectivity, reproducibility (RSD 3.6%), and recovery rate (95.2–102.0%) are all satisfactory. The practicability of the sensor was verified by detecting IgG in human serum samples, which indicated that the sensor was suitable for potential clinical applications.
研究领域:
分析化学
·
生物传感器
·
材料科学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 109
·107732-107738
·
IF 3.289
Zhifen Deng,Kai Hu,Liangliang Bi,Hang Yuan,Yanlong Chen,Shengnan Zhao,Huifang Du,Xuesheng Yuan,Yanjie Huang,Shusheng Zhang
Selective removal of highly abundant proteins prior to analysis is an important issue in proteomics. In this study, a core–shell structured magnetic nanocomposite of Fe3O4@SiO2@MAsp-HEMA was found to be an effective and selective adsorbent to remove highly abundant IgG from the urine of patients with proteinuria. This novel magnetic adsorbent was prepared by coating a poly(N-methacryloyl-L-aspartic acid-hydroxyethyl methacrylate) shell on Fe3O4@SiO2 nanoparticles via a coupling agent of KH-570 using the mini-emulsion polymerization method. The as-synthesized nanoparticle was characterized by X-ray diffraction, elemental analysis, thermal gravimetric analysis, infrared spectroscopy, magnetic hysteresis loop curves, scanning electronic microscopy and transmission electron microscopy. A kinetic adsorption experiment was performed to investigate the speed of adsorption and the time to reach the adsorption balance. 1D SDS-PAGE analysis and capillary electrophoresis were used to validate its selective adsorption ability. The resulting Fe3O4@SiO2@MAsp-HEMA showed a uniform spherical shape at nanoscale dimensions of about 200–300 nm, removing efficiency above 80%, and maximum adsorption capacity of 18.08 μg mg−1 at 15 ± 0.5 °C. The binding between IgG and the ligand was confirmed by isothermal titration calorimetry. The hydrogen bonding force was demonstrated as one of the major interactions between the functional groups of the as-synthesized magnetic nanoparticles and IgG by a molecular docking experiment.
研究领域:
蛋白质组学
·
纳米技术
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）