Nanoscale
·
2024
·
IF 5.8
Jiayi Zhu,Chao Zhao,Binyun Xia,Ning Wang,Xi Chen,Xinyue Jing,Minxuan Chen,Xinrui Xu
Abnormal human immunoglobulin G (IgG) may induce the risk of immune system disorder, infectious diseases, tumors and so on. However, the current detection methods exhibit low sensitivity, which limits their practical application. In this work, an SPR optical fiber sensor (SPR-OFS) with high sensitivity is designed for label-free detection of human IgG. It is fabricated using a heterostructure optical fiber coated with Au film/AuNPs and the Ti3C2Tx MXene biofunctionalized with goat anti-human IgG by polydopamine (PDA). In the experiment, the optimal thickness of the Ti3C2Tx MXene was explored and determined to be about 93 nm by comprehensively considering the refractive index (RI) sensitivity and spectral bandwidth of the SPR sensor. When the largest figure of merit (FOM) is calculated to be 17.8279 RIU−1, its RI sensitivity was ultimately found to be 2804.5 nm per RIU. The SPR-OFS was employed to detect human IgG within the concentration range of 0–30 μg mL−1 and its sensitivity is demonstrated to be 1.7046 nm (μg mL−1)−1. The SPR-OFS was also proved to have excellent linearity, specificity and stability. The proposed sensor offers outstanding performance with simple fabrication, providing a cutting-edge bioanalytical platform with potential applications in clinical diagnosis.
研究领域:
生物传感技术
·
医学诊断
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 28
·3411-3419
·
IF 3.1
Jiajia Wang,Wenhui Dong,Xiaomin Yang,Yanan Li,Baokang Jin
Electrochemical detection of human-IgG via biosensors is vital in clinical diagnostics, owing to their simple equipment, facile operation, high selectivity, economical, short diagnostic time, fast response, and easy miniaturization, but the need to improve sensitivity for protein detection is still a barrier limiting its wider practical applications. A hypersensitized electrochemical biosensor based on steric effects for IgG detection was developed in this work. The results indicate that IgG-modified sig-DNA attached to CdTe quantum dots (CdTe-sig-DNA) limited the ability of CdTe-sig-DNA or CdTe-sig-DNA-IgG conjugate to hybridize through the captured DNA strand (cap-DNA) immobilized on a chitosan/nitrogen-doped carbon nanocomposite (CS/N-C) modified glassy carbon electrode surface (GCE). The concentration of IgG based on CdTe concentration was detected by differential pulse anode stripping voltammetry (DPASV) on the electrode surface. The efficiency for hybridizing CdTe-sig-DNA with cap-DNA was found to be logarithmically inverse to the concentration of IgG attached. A highly sensitive and selective detection of IgG from 5 pM to 50 μM with a relatively low detection limit of 1.7 pM was achieved. Therefore, the steric hindrance effect of IgG limited the quantity of DNA that could be functionalized on CdTe QDs, significantly improving the signal, and providing a practical strategy for the clinical analysis of IgG.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS
·
2025
·Vol. 25
·期 5
·1630
·
IF 3.5
Qiang Xu,Huiting Yin,Mei Cui,Renliang Huang,Rongxin Su
A fiber-reinforced SPR sensor based on silver-nucleated gold-shell bimetallic nanoparticles and graphene oxide was developed and applied to human IgG detection. The refractive index (RI) sensitivity of the Ag@Au/GO fiber SPR sensor is as high as 4715.9 nm/RIU in the RI range of 1.333–1.365. Staphylococcus aureus protein A (SPA) can specifically recognize and bind to the fragment crystallizable (Fc) of the antibody; it facilitates the highly targeted immobilization of the antibody. SPA and rabbit anti-human IgG were immobilized on the surface of the Ag@Au/GO fiber SPR sensor for the detection of different concentrations of human IgG with a sensitivity of 0.53 nm/μg/mL and detection limits of 0.037 μg/mL. This biosensor based on the mixed structure of GO and Ag@Au combined the common advantages of the two materials. Therefore, our study provides a simple platform for biological analysis and has a good application prospect.
研究领域:
生物传感技术
·
材料科学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2015
·
IF 2.219
Chunxiang Zhang,Guangyu Shen,Youming Shen,Xiangyang Zhang
In this work, a novel thiol aromatic aldehyde was synthesized. It can be used as a substrate to directly immobilize antibodies on a gold electrode, for which no additional chemical cross-linker is required. It was also applied as a linker to prepare Fe 3 O 4 @Au/PAMAM/Ab 2 –horseradish peroxidase bioconjugates , which introduced multiple enzymes onto a sensing interface owing to the high surface-to-volume ratio of Fe 3 O 4 @Au nanoparticles and many functional groups of the poly(amidoamine) dendrimer (PAMAM). The introduced multiple enzymes greatly improved the detection signal. Under optimal conditions, the proposed electrochemical immunosensor exhibited desirable performance for detection of IgG in the range 0.005–50 ng ml −1 with a detection limit of 3 pg ml −1 based on a signal-to-noise ratio of 3. It has great potential application in the area of clinical analysis.
研究领域:
生物医学工程
·
分析化学
·
纳米技术
✓ 已验证
DOI
高影响因子
OPTICS EXPRESS
·
2020
·Vol. 28
·期 8
·12233-12242
·
IF 3.669
Xiaoyu Shi,Kun Ge,Jun-Hua Tong,Tianrui Zhai
Low-cost and miniaturized biosensors are key factors leading to the possibility of portable and integrated biomedical system, which play an important role in clinical medicine and life sciences. Random lasers with simple structures provide opportunities for detecting biomolecules. Here, low-cost biosensors on fiber facet for label-free detecting biomolecules are demonstrated based on a plasmonic random laser. The random laser is achieved resorting to a self-assembled plasmonic scattering structure of Ag nanoparticles and polymer film on fiber facet. Refractive index sensitivity and near-surface sensitivity of the biosensor are systematically studied. Furthermore, the biosensor is used to detect IgG through specific binding to protein A, exhibiting the detecting limit of 0.68 nM. It is believed that this work may promote the applications of a plasmonic random laser bio-probe in portable or integrated medical diagnostic platforms, and provide fundamental understanding for the life science.
研究领域:
生物医学工程
·
纳米技术
·
光学传感
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Qi Wang,Bo-Tao Wang
A graphene oxide/silver coated polymer cladding silica fiber optical surface plasmon resonance (SPR) human IgG detection biosensor with a high sensitivity and a low limit of detection is presented. Due to the graphene oxide (GO), the intensity of the confined electric field surrounding the sensing layer can be enhanced, the sensitivity of refractive index (RI) of the graphene oxide/silver SPR sensor reached 3311 nm/RIU in the RI range of 1.3334–1.3731 compared with 2875 nm/RIU in the range of 1.3328–1.3739 of the silver film SPR sensor. Afterwards, SPR optical fiber biosensor was developed based on graphene oxide/silver coated optical fiber when staphylococcal protein A and goat anti-human IgG were immobilized on the surface of the graphene oxide film. The sensor can be used to detect different concentrations of human IgG, a high sensitivity of 0.4985 nm/(μg/mL) and a low limit of detection 0.04 μg/mL were observed. Therefore, the graphene oxide/silver film SPR human IgG detection biosensor with high sensitivity and low limit of detection shows a great promise for the future biochemical and biomedical application.
研究领域:
生物传感技术
·
医学检测
·
光学工程
✓ 已验证
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 24
·10850-10856
·
IF 3.591
Xiaoyan Chen,Lin Song,Guanyang Zhu,Jing Ma,Ajing Xu,Wenya Zhao,Yingying Gu,Yarui An,Yuqing Miao
In this work, a unique zirconium dioxide solid superacid (ZrO2/SO42−) was utilized for the fabrication of an IgG electrochemical immunosensor. The treated ZrO2 formed a SO bond on the surface, which has the electron-attracting induction effect and can further enhance the quantity and stability of immobilized biomolecules. In addition, using a platinum nanoparticle–chitosan–reduced graphene oxide (PtNP–CHIT–rGO) nanocomposite as a substrate modification material not only improves the conductivity of the electrode, but also increases the effective surface area of the immunosensing platform. The proposed label-free immunosensor has a lower detection limit of 11.0 pg mL−1 and a wide linear range of 0.01–10.0 ng mL−1 and 10.0–4000.0 ng mL−1. Importantly, the immunosensor exhibits good selectivity, excellent stability and reproducibility, and is expected to be applied to the detection of other biomolecules.
研究领域:
生物化学
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2019
·Vol. 43
·期 36
·14220-14223
·
IF 3.069
Kai-Xin Xie,Shu-Shu Jia,Jin-Hua Zhang,Huan Wang,Qiang Wang
The enhancement of surface plasmon-coupled emission (SPCE) by CNT assistance was investigated with the factor of 12 and 65 as compared to normal SPCE and free space emission, respectively. This strategy designed as an immunosensor to detect IgG with a detection limit of 0.0025 ng mL−1 provides a universal way to improve the detection sensitivity in fluorescence-based sensing platforms.
研究领域:
纳米技术
·
光学
·
分析化学
·
生物传感器
✓ 已验证
产品关联
DOI
高影响因子
OPTICS AND LASER TECHNOLOGY
·
2018
·
IF 2.503
Qi Wang,Botao Wang
A sensitivity enhanced surface plasmon resonance (SPR) immunosensor based on Graphene oxide (GO) composite and staphylococcal protein A (SPA) co-modified photonic crystal fiber (PCF) is developed for human IgG detection. The PCF spliced between two section of multimode fibers (MMFs) is sputtered with gold film, which was then modified with graphene oxide and SPA for further immunosensing. Graphene oxide with abundant functional groups has a large surface area for more biomolecules immobilization and can enhance the interaction between Au film and external medium, leading to the sensitivity increased greatly. SPA can specifically bind the Fc region of the antibodies and has a high degree of orientation for capturing antibodies. Experimental results indicated that the refractive index (RI) sensitivity of graphene oxide modified Au-PCF SPR sensor reaches 4649.8 nm/RIU, which is about 1888 nm/RIU higher than that without graphene oxide film and the human IgG detection limit reaches as low as 10 ng/mL. Such a sensitivity enhanced immunosensor based on graphene oxide and SPA co-modified PCF shows a great promise in biosensing.
研究领域:
生物传感
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Chemistry
·
2024
·
IF 3.8
Zhang Lizhong,He Mingyi,Xu Yang,Guo Cuixia,Zhou Chongqi,Guan Tian
An ultra-sensitive phase plasmonic sensor combined with weak value amplification is proposed for the detection of IgG, as a model analyte. Phase detection is accomplished by self-interference between the p-polarization and the s-polarization of the light. With the principles of weak value amplification, a phase compensator is used to modulate the coupling strength and enhance the refractive index sensitivity of the system. On a simple Au-coated prism-coupled surface plasmon resonance (SPR) structure, the scheme, called WMSPR, achieves a refractive index sensitivity of 4.737 × 104 nm/RIU, which is about three times higher than that of the conventional phase-based approach. The proposed WMSPR biosensor gives great characteristics with a high resolution of 6.333 × 10−8 RIU and a low limit of detection (LOD) of 5.3 ng/mL. The results yield a great scope to promote the optimization of other SPR biosensors for high sensitivity.
研究领域:
光学
·
生物传感
·
纳米技术
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Ling-Xin Kong,Yan-Xin Zhang,Wei-Gang Zhang,Yun-Shan Zhang,Tie-Yi Yan,Peng-Cheng Geng,Biao Wang
Lab-on-tip technology opens a new door for optical fiber surface plasmon resonance (SPR) sensors. In this paper, we first demonstrate an ultra-compact reflective SPR microtip probe on the flat fiber end in experiment, which has a length of tens of microns and a diameter of several microns. Simulations and experiments show that this new fiber SPR microtip probe coated with a nano-goldfilm has excellent refractive index (RI) sensing characteristic and great potential for biochemical detection at single cell level. Based on the high RI sensitivity, a bio-probe assembled with Chitosan/Polysodium Styrene Sulfonate (Cs/PSS) nano-polyelectrolyte film and the sheep anti-human IgG-HRP is prepared to recognize human IgG specifically. Due to the high surface sensitivity, the SPR microtip probe exhibits an ultra-low detection limit for human IgG. Due to the high bulk sensitivity and high thermo-optical coefficient (TOC) of polydimethylsiloxane (PDMS), a reference temperature probe based on SPR microtip is proposed, which is used to compensate for the temperature cross-sensitivity of the bio-probe. This new SPR microtip probe can be used not only in optical fiber sensor, but also in Tip-Enhanced Near-Field Raman Microscopy and Scanning Near-Field Optical Microscope.
研究领域:
光纤传感器
·
表面等离子体共振(SPR)技术
·
生化传感
·
纳米技术
·
显微镜
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）