LWT-FOOD SCIENCE AND TECHNOLOGY
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2024
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IF 6
Yun Zhang,Yibing Zhang,Ruihua Lun,Qingshan Fu,Yuqiao Chang,Jiansen Du,Yi Zhang,Junping Yu
Conventional methods for Staphylococcus aureus ( S. aureus ) detection using mammalian immunoglobulins could yield false positive results, owing to the presence of the protein G-producing Streptococcus . In this study, because canine IgM can bind to protein A but not to protein G, we firstly designed a colorimetric platform based on canine IgM to identify S. aureus . Magnetic beads (MBs) functionalized with canine IgM acted as captors to enrich S. aureus , relying on the binding interaction between the Fc region of canine IgM and protein A in the bacterial cell wall, while horseradish peroxidase (HRP)-labeled rabbit anti- S. aureus IgG served as a tracer in the sandwich-type immunoassay, catalyzing the colorimetric reaction. Under optimal conditions, the proposed strategy achieved a satisfactory analytical performance with a broad linear range from 1.6 × 10 3 CFU/mL to 1.0 × 10 5 CFU/mL, a colorimetric minimum resolution of as low as 1.4 × 10 2 CFU/mL in 100 μL phosphate-buffered saline (PBS), and good selectivity in differentiating target from protein G-producing Streptococcus . Moreover, the proposed approach successfully identified S. aureus in various application scenarios (except for samples containing IgG), can be completed in less than 90 min, and was not dependent on any specialized equipment.
研究领域:
微生物学
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免疫学
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分析化学
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2024
·
IF 6.1
Yongwei Zhang,Ziyue Li,Aolin Zhu,Qian Zhang,Hao Zhou,Xuelei Zhou,Tingwei Liu,Chunyan Liu,Hefei Zha,Xin Zhang,Hui Zhao,Jiutong Li,Guodong Lü,Xinxia Li
Brucellosis is a bacterial infectious disease caused mainly by Brucella. Transmission is mainly by contact with infected domestic or wild animals or their excreta. Clinical diagnosis of brucellosis is usually based on qualitative total antibody tests, which make it difficult to differentiate between acute and pre-existing infections. In this study, for the simultaneous detection of anti-brucellosis IgG and IgM, we innovatively developed a surface-enhanced Raman scattering (SERS)-lateral flow immunoassay (LFIA) detection system, and evaluated its performance and diagnostic effect by using clinical serum samples. The key design of this biosensor involves the preparation of two immunoprobes using two Raman spheres (R-Sphere) with distinct signals conjugated to mouse anti-human IgG and IgM, respectively. In this design, brucellosis-specific antigens are embedded in the T-line, allowing simultaneous capture of anti-brucellosis IgG and IgM in a specific binding reaction. A portable Raman instrument is used to detect the characteristic signal intensities generated by IgM and IgG on the T-line, which can then be used to analyze the target IgM and IgG antibodies. Based on the analysis of 40 clinical samples, the method has a sensitivity and specificity of 100%, and the detection time is only 15 min. The advantages of this technology are fast speed, convenient use, high sensitivity, and it can distinguish the disease course to achieve precise treatment. These features make it an ideal solution for large-scale brucellosis testing in remote and nomadic areas, where it can play a crucial role in improving public health protection.
研究领域:
医学诊断
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生物传感技术
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传染病研究
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产品关联
DOI
PubMed
高影响因子
Frontiers in Immunology
·
2019
·
IF 4.716
Wu Liting,Kong Linghe,Yang Yanjian,Bian Xia,Wu Siwei,Li Bingxi,Yin Xiaoxue,Mu Liangliang,Li Jun,Ye Jianmin
Teleost B cells have phagocytic activities for ingesting particulate antigens, such as bacteria, in addition to the functional secretion of immunoglobulins (Igs). In the present study, the phagocytic activities of IgM+ B cells under various differentiational conditions residing in peripheral blood leukocytes were investigated in a teleost fish Nile tilapia (Oreochromis niloticus). The IgM+ B cells were recognized as IgMlo or IgMhi subsets based on their membrane IgM (mIgM) levels. The mIgM, secreted IgM (sIgM), major histocompatibility complex class II and reactive oxygen species were detected. Expressions of transcription factors (Pax5 and Blimp-1) and B cell signaling molecules (CD79a, CD79b, BLNK, and LYN) suggested that IgMlo B cells were resembling as plasma-like cells and IgMhi resembling as naïve/mature B cells, respectively. Analysis of phagocytic activities demonstrated that both IgMlo and IgMhi B cells have a similar phagocytic ability (phagocytosis percentage); however, the phagocytic capacity [phagocytic index and the mean fluorescence intensity (MFI)] of IgMhi B cells was significantly higher than that of IgMlo B cells. Taken together, the results indicated that B cell differentiation may cause the decrease of phagocytic capacity but not phagocytic ability of phagocytic IgM+ B cells in teleost. The finding may provide an evolutionary evidence for understanding the greater specialization of the B cell in more sophisticated adaptive humoral immunity, by decreasing phagocytic activity in order to contribute its function more specifically into antibody-secreting.
研究领域:
免疫学
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海洋生物学
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细胞生物学
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进化生物学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2022
·
IF 6.064
Ju Jian,Zhang Xinyu,Li Lin,Regmi Sagar,Yang Guoqiang,Tang Shixing
Understanding the dynamic changes in antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for evaluating the effectiveness of the vaccine and the stage for the recovery of the COVID-19 disease. A rapid and accurate method for the detection of SARS-CoV-2-specific antibodies is still urgently needed. Here, we developed a novel fluorescent lateral flow immunoassay (LFA) platform for the detection of SARS-CoV-2-specific IgM and IgG by the aggregation-induced emission carbon dots conjugated with the SARS-CoV-2 spike protein (SSP). The aggregation-induced emission carbon dots (AIE-CDs) are one of the best prospect fluorescent probe materials for exhibiting high emission efficiency in both aggregate and solid states. The AIE-CDs were synthesized and displayed dual fluorescence emission, which provides a new perspective for the design of a high sensitivity testing system. In this work, the novel LFA platform adopted the AIE carbon dots, which are used to detect SARS-CoV-2-specific IgM and IgG conveniently. Furthermore, this sensor had a low LOD of 100 pg/ml. Therefore, this newly developed strategy has potential applications in the areas of public health for the advancement of clinical research.
研究领域:
医学诊断
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生物化学
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纳米技术
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公共卫生
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产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2019
·
IF 3.298
Liting Wu,Yanjian Yang,Linghe Kong,Xia Bian,Zheng Guo,Shengli Fu,Fang Liang,Bingxi Li,Jianmin Ye
In teleost fish, IgM + B cells play important roles in innate and adaptive immunity. Different IgM + B cells are detected in teleost, named IgM lo and IgM hi B cell subsets , according to the distinct expression levels of membrane IgM (mIgM). However, the study on the heterogeneity in IgM + B cell subsets remains poorly understood. In this study, the comparative transcriptomic profiles of IgM − , IgM lo and IgM hi from peripheral blood of Nile tilapia ( Oreochromis niloticus ) were carried out by using RNA-sequencing technique. A total of 6045 and 5470 differentially expressed genes (DEGs) were detected in IgM lo and IgM hi cells, respectively, as compared with IgM − lymphocytes, whereas 3835 genes were differentially expressed when IgM lo compared to IgM hi cells. Analysis of the KEGG database indicated that the DEGs were enriched in immune system categories and signaling transduction and interaction in IgM − vs IgM hi , IgM − vs IgM lo and IgM lo vs IgM hi . Comparatively, in IgM lo vs IgM hi , GO enrichment analysis indicated that the DEGs enriched in nucleic acid binding transcription factor activity. Analysis of crucial transcription factors for B cell differentiation indicated that IgM lo and IgM hi cell clusters belonged to the different B cell subsets. The data generated in this study may provide insights into understanding the heterogeneity of IgM + cells in teleost, and suggest that IgM + B cells play a crucial role in innate immunity .
研究领域:
免疫学
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转录组学
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鱼类生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
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2022
·
IF 5.76
Zixuan Liu,Zheng-Zhi Yin,Guojun Zheng,Hongyu Zhang,Min Zhou,Shan Li,Yong Kong
Detection of immunoglobulins (Igs) is of clinical significance for early diagnosis and timely treatment of diseases. Herein, a dual-template molecularly imprinted (DTMI) electrochemical biosensor was developed for IgG-IgM combined assay. In this DTMI electrochemical biosensor, Prussian blue (PB) and thionine (TH) decorated on graphene oxide (GO) and multi-walled carbon nanotubes (MWCNTs), respectively, were utilized as the dual-signal probes, and Au nanoparticles (AuNPs) were used for Igs anchoring and signal amplification. Polypyrrole (PPy) was electrodeposited on the biosensor surface and acted as the molecularly imprinted polymers (MIPs). After the removal of the IgG and IgM templates, the resultant DTMI electrochemical biosensor was used for IgG-IgM combined assay, and the concentrations of IgG and IgM could be indicated by the changes in the peak currents of PB (Δ I PB ) and TH (Δ I TH ), respectively. The DTMI electrochemical biosensor displayed a wide linear range and a low limit of detection (LOD) for both IgG (28.80 pg mL −1 ) and IgM (0.58 pg mL −1 ). Finally, the developed DTMI biosensor was used for IgG-IgM combined assay in clinical serum samples, and the results were comparable to those obtained by conventional immunoturbidimetry, implying its great potential in clinical diagnosis.
研究领域:
分析化学
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生物传感器
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电化学
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纳米技术
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临床诊断
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产品关联
DOI
PubMed
高影响因子
Frontiers in Immunology
·
2022
·
IF 8.786
Wu Liting,Yang Yanjian,Gao Along,Li Jun,Ye Jianmin
Plasma cells are terminally differentiated antibody-secreting B lymphocytes that contribute to humoral immunity by producing large numbers of antibodies. Increasing evidence suggests that teleost fish B cells share certain characteristics with mammalian B1 B cells, including antibody-secreting, phagocytic, and antigen-presenting capacities. However, the difference between mature B cells and plasma cells remains unclear. In this study, we found that, based on their light-scattering characteristics, tilapia anterior kidney (AK) leukocytes can be categorized into two IgM+ B-cell subsets: the lymphoid (L) gate and granulocyte–monocyte/macrophage (G-M) subsets. G-M gate cells are more numerous than L-gate cells and have higher mean fluorescence, but lower forward scatter and side scatter. We analyzed the morphological and ultrastructural features of sorted IgM+ cells and found that L-gate IgM+ cells have a high nucleus–cytoplasm ratio and lymphocyte-like morphology, whereas G-M gate IgM+ cells have a small nucleus, more abundant endoplasmic reticulum, and a larger number of mitochondria, and have a plasma cell-like or macrophage-like morphology. To further characterize the cell types, we examined the specific patterns of expression of B-cell- and T-cell-related genes. We found that B-cell-specific genes were expressed by both L-gate and G-M gate IgM+ cells, and that G-M gate IgM+ cells secreted extremely high levels of IgM. However, T-cell-related genes were highly expressed only in L-gate IgM– cells. These results suggest that G-M gate IgM+ cells are similar to plasma-like cells, with high antibody-secreting capacity. Given that G-M gate cells include the granulocyte, monocyte, and macrophage cell types, but not B cells, monocyte/macrophage markers were used to investigate the cell types further. A macrophage receptor with a collagenous structure was frequently observed, and macrophage-expressed gene-1 was highly expressed, in the G-M gate IgM+ cells. Phagocytic capacity, as determined by ingestion of beads or bacteria, was significantly higher in G-M gate IgM+ cells than in L-gate IgM+ cells, as was antigen-processing capacity. Our findings show that tilapia AK leukocytes can be divided into two IgM+ B-cell subsets and that G-M gate IgM+ cells resemble plasma-like cells, having high antibody-secreting, phagocytic, and antigen-presenting capacities. Thus, this study increases our understanding of the functions of teleost fish plasma-like cells.
研究领域:
免疫学
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鱼类生物学
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细胞生物学
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DOI
PubMed
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 47
·28041-28048
·
IF 3.119
Caixia Liu,Baiping Mao,Vanessa Martinez,Xiaojian Chen,Yanan Li,Lingyun He,Sian Chen,Xiaoling Guo,Xian Shen,Xiandan Bao,Haifa Shen,Stefania Lenna,Pinyi Qian,Lingzhi Wu,Chao Li
The outbreak of new coronavirus disease (COVID-19) has quickly spread all over the world. Real time reverse transcriptase polymerase chain reaction (rRT-PCR) for nucleic acid detection has become the standard method for clinical diagnosis of COVID-19 infection. But these rRT-PCR tests have many inherent limitations, and carry a high false negative rate. It is an urgent to develop a method to accurately identify the vast infected patients and asymptomatic viral carriers from the population. In this article, we present the principle and procedure of developing a colloidal gold immunochromatographic assay (GICA) for rapid detection of COVID-19-specific antibodies. The detection kit can be used to detect immunoglobulin M (IgM) and IgG of COVID-19 in human blood samples within 15 minutes, and to identify different stages of viral infection. Test results can be digitalized using an office scanner and a FiJi software with appropriate confidence interval (CI) setting. Based on analysis from 375 samples, we calculated that overall sensitivity and specificity of the assay were 95.85% and 97.47%, respectively. Compared with rRT-PCR, this assay has many advantages including convenience and rapid detection. The detection kit can be widely used in hospitals, clinics and laboratories for rapid screening of both symptomatic and asymptomatic COVID-19 carriers in large scale.
研究领域:
医学
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病毒学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 3
·1-10
·
IF 5.3
Wang Jing,Zhang Liang,Yan Guanrong,Cheng Linfeng,Zhang Fanglin,Wu Jialin,Lei Yingfeng,An Qunxing,Qi Honglan,Zhang Chengxiao,Gao Qiang
An enzyme immunoassay was developed based on the coulometric measurement of immunoglobulin M (IgM) against Hantaan viruses (HTNV) by using virus-like particles (VLPs) as recognition molecules. The surface functionalization of screen-printed carbon electrodes (SPCEs) was achieved through paste-exfoliated graphene that was modified with a COOH group and a thionine mediator through supramolecular-covalent scaffolds, on SPCEs by using the binder contained in the ink. After the covalent immobilization of the antibody, the sensor was used for the sandwich enzyme immunoassay of IgM against HTNV. By using HTNV VLPs as the second recognization molecules, the resulting sensor efficiently monitored the reaction of IgM against HTNV and anti-IgM antibody with high specificity. By attaching HTNV nucleocapsid protein antibody conjugate with horseradish peroxidase (HRP) onto VLPs, the signal response of the assay was derived from the coulometric measurement of H 2 O 2 reduction mediated by thionine on the electrode surface after the application of a potential (− 0.2 V vs. Ag/AgCl). The ratio of charges measured before or after H 2 O 2 addition was used to quantify IgM because these charges could be used as background charges or total charges, respectively. The ratio exhibited good agreement with IgM concentration within a range 0.1 to 1000 pg mL −1 , and a detection limit of 0.06 pg mL −1 was obtained. The assay demonstrated high sensitivity and specificity toward HTNV-specific IgM in serum. Graphical abstract
研究领域:
生物医学工程
·
分析化学
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DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Qiaoge Bayin,Lei Huang,Chunhui Ren,Yusheng Fu,Xing Ma,Jinhong Guo
Since December 2019, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has caused millions of deaths and seriously threatened the safety of human life; indeed, this situation is worsening and many people are infected with the new coronavirus every day. Therefore, it is very important to understand patients’ degree of infection and infection history through antibody testing. Such information is useful also for the government and hospitals to formulate reasonable prevention policies and treatment plans. In this paper, we develop a lateral flow immunoassay (LFIA) method based on superparamagnetic nanoparticles (SMNPs) and a giant magnetoresistance (GMR) sensing system for the simultaneously quantitative detection of anti -SARS-CoV-2 immunoglobulin M (IgM) and G (IgG). A simple and time-effective co-precipitation method was utilized to prepare the SMNPs, which have good dispersibility and magnetic property, with an average diameter of 68 nm. The Internet of Medical Things-supported GMR could transmit medical data to a smartphone through the Bluetooth protocol, making patient information available for medical staff. The proposed GMR system, based on SMNP-supported LFIA, has an outstanding advantage in cost-effectiveness and time-efficiency, and is easy to operate. We believe that the suggested GMR based LFIA system will be very useful for medical staff to analyze and to preserve as a record of infection in COVID-19 patients.
研究领域:
医学诊断
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生物传感技术
·
传染病研究
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产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2026
·
IF 4.2
Liwen Zhu,Chanchan Lin,Jing Cen,Wa Gao,Yueshuang Ji,Jing Xu,Hao Feng,Zhen Xu,Carolina Tafalla,Jun Zou,Junya Wang
A monoclonal antibody against native grass carp IgM showed cross-reactivity with multiple cyprinid species. • Bacterial and viral infections induced expansion and local aggregation of splenic IgM + B cells in two cyprinid species. • Bacterial and viral infections promoted the proliferation of splenic IgM + B cells in two cyprinid species. Humoral immunity is a central component of host defense in teleost fish, yet the dynamics of IgM + B cells during infection remain poorly understood. In this study, we generated and characterized a monoclonal antibody (mAb) against native IgM protein purified from grass carp ( Ctenopharyngodon idella ) sera. This mAb specifically recognized the recombinant grass carp IgM heavy chain expressed in the HEK293 cells and showed cross-reactivity with IgM molecules from multiple cyprinid species, including rare minnow, zebrafish, black carp, and common carp, but not with IgM from largemouth bass. Flow cytometric analysis showed that IgM + B cells were abundant in the spleen and head kidney of grass carp, accounting for 35.40 ± 3.15% and 27.63 ± 3.97% of total leukocytes, respectively. Using this antibody, we further investigated IgM + B-cell responses in grass carp and rare minnow after infection with Aeromonas hydrophila or grass carp reovirus genotype II. Both bacterial and viral infections significantly enhanced the proliferation and local aggregation of splenic IgM + B cells. Moreover, EdU + proliferating cells were preferentially enriched within localized IgM + B-cell clusters in the spleens of infected fish. Taken together, our work provides a useful antibody tool for studying B-cell responses and reveals that bacterial and viral infections induce proliferation and localized aggregation of splenic IgM + B cells in teleost fish, highlighting infection-induced remodeling of splenic IgM + B-cell responses.
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DOI
高影响因子
ACS Omega
·
2020
·
IF 2.87
Jiaping Guo,Yameng Wang,Shanshan Niu,Hongping Li,Yongmei Tian,Songcheng Yu,Fei Yu,Yongjun Wu,Li-e Liu
The aim of this study is to establish a new method with high sensitivity, accuracy, and stability for the determination of human IgG and then expand it to analyze severe acute respiratory syndrome corona virus 2 (SARS-CoV-2)-specific IgM and IgG, which is of great significance for the screening and diagnosis of COVID-19. In this study, the magnetic Fe3O4 nanospheres coupled with mouse antihuman IgG (Ab1IgG) were used as an immune capture probe (Fe3O4@Ab1IgG) to capture and separate the target, and rabbit antihuman IgG (Ab2IgG) coupled with highly luminescent quantum dot nanobeads (QBs) as a fluorescence detection probe (QBs@Ab2IgG) was used to realize high sensitivity detection. After the formation of a sandwich immunocomplex, the fluorescence intensity of the precipitate after magnetic separation was measured at the excitation wavelength of 370 nm. Under optimal conditions, a wide linear range varying from 0.005 to 40 ng·mL–1 was obtained for the detection of human IgG with a lower limit of detection at 4 pg·mL–1 (S/N = 3). The recoveries of intra- and interassays were 90.0–101.9 and 96.0–106.6%, respectively, and the relative standard deviations were 6.3–10.2 and 2.6–10.5%, respectively. Furthermore, the proposed method was successfully demonstrated to detect human IgG in serum samples, and the detection results were not statistically different (P > 0.05) from commercial enzyme-linked immunosorbent assay kits. This method is sensitive, fast, and accurate, which could be expanded to detect the specific IgM and IgG antibodies against SARS-CoV-2.
研究领域:
生物化学
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纳米技术
·
分析化学
·
免疫学
·
病毒学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）