MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 7
·1-11
·
IF 6.232
Mao Xiaoxia,Liu Shaowei,Su Benyue,Wang Dejin,Huang Zhan,Li Jian,Zhang Yuanguang
A luminescent metal-organic framework of type Eu(III)-MOF has been fabricated for visual and on-site fluorometric determination of hydrogen peroxide (H 2 O 2 ) via a tablet computer. The maximum excitation and emission peaks of type Eu(III)-MOF were found at λex = 290 nm and λem = 615 nm, respectively. The average length of Eu-MOF is 1.21 ± 0.07 μm. In the presence of the target H 2 O 2 , Fe 2+ is transmitted into Fe 3+ via Fenton reaction, leading to a fluorescence quenching of Eu-MOF. Therefore, visible color change occurred from bright red into colorless. Interestingly, by means of tablet computer’s digital camera and ImageJ software, fluorescent signals were captured and transduced into digital parameters, resulting in a linear relationship between fluorescence intensity and the concentration of H 2 O 2 . As a result, the determination of H 2 O 2 without the aid of complicated instruments is achieved in the range 2.0 μM to 0.2 mM with a detection limit of 1.02 μM. Our approach has been successfully applied to quantify H 2 O 2 in serum, urine, and waste water with good recovery and precision (< 2.5% RSD). Besides, our assay has been exploited for visual detection of H 2 O 2 released from HepG2 cells with the advantages of portability and accuracy. Moreover, the strategy displays acceptable selectivity and stability. Hence, our assay provides an alternative practical method for on-site determination of H 2 O 2 without the need for instruments.
Schematic representation of the synthesis procedure of a luminescent Eu-MOF, which has been successfully applied for on-site detection of H 2 O 2 via Fenton reaction and imaging analysis technique. The method exhibits handheld and accuracy for H 2 O 2 determination, holding the potential for biochemical and clinical applications in remote regions.
研究领域:
分析化学
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生物化学
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材料科学
·
临床诊断
✓ 已验证
DOI
PubMed
高影响因子
FOOD CONTROL
·
2022
·
IF 6.652
Gaozhen Li,Fukai Guan,Shancang Zhao,Huihui Xu,Jiashuai Sun,Jingcheng Huang,Qingqing Yang,Yanyin Guo,Xia Sun,Yemin Guo
As an organochlorine pesticide, procymidone has become an increasing serious problem in vegetables in recent years. In order to solve a series of problems such as low sensitivity of the immunological methods, a time-resolved fluorescent immunoassay strip (TRFIAS) was developed with low limit of detection (LOD). Based on the principle of antigen-antibody immune competition, TRFIAS could detect procymidone in vegetables. After the targets being added, the samples were incubated at 37°C for 10 min. TRFIAS was observed under a UV lamp of 365 nm. The results were processed by ImageJ software to complete the qualitative and quantitative analysis of TRFIAS. Under the optimal working conditions, a linear range of TRFIAS was 5–250 ng/mL with a LOD of 0.33 ng/mL and a visual limit of detection (VDL) of 50 ng/mL. And the TRFIAS had good specificity, precision and accuracy. Comparing the results of TRFIAS with those of ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), the two assays were in good agreement. Therefore, TRFIAS has the advantages of high sensitivity, high specificity, simple operation and relatively short assay time. Meanwhile, it shows absolutely great advantages in both quantitative and qualitative analysis.
研究领域:
农业化学
·
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Rui Jin,Deshuai Kong,Xu Yan,Xu Zhao,Hongxia Li,Fangmeng Liu,Peng Sun,Yuehe Lin,Geyu Lu
Precise on-site profiling of organophosphate pesticides (OPs) is of significant importance for monitoring pollution and estimating poisoning. Herein, we designed a simple and convenient portable kit based on Ag+-responsive hydrogels for accurate detection of OPs. The newly developed hydrogels employed o-phenylenediamine (OPD) and silicon quantum dots (SiQDs) as indicator, which possessed ratiometric response. In this sensor, OPs as inhibitor of acetylcholinesterase prevented the generation of thiocholine, which blocked the formation of metal–polymer with Ag+, further triggered the oxidation of OPD to yield yellow 2,3-diaminophenazine (DAP) with fluorescence emission at 557 nm. The fluorescence intensity of SiQDs (444 nm) was quenched by DAP through inner filter effect (IFE) process, emerging a typical ratiometric response. Interestingly, the ratiometric signal of kit, which was recorded by smartphone’s camera, can be transduced by ImageJ software into the hue parameter that was linearly proportional to the concentration of OPs. The simplicity of portable kit combined with smartphone operation, which possessed high sensitivity (detection limit <10 ng mL–1) and rapid sample-to-answer detection time (45 min) in agricultural sample, indicating that the methodology offered a new sight for portable monitoring of food safety and human health.
研究领域:
分析化学
·
环境科学
·
材料科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Siyang Deng,Junmei Liu,Dong Han,Xinting Yang,Huan Liu,Chunhui Zhang,Christophe Blecker
A novel ratiometric fluorescent nanoprobe (Rh6G@UIO-66-NH 2 ) was fabricated for efficient nitrite (NO 2 – ) detection in the present study. When NO 2 – was introduced, it interacted with the amino groups on the surface of Rh6G@UIO-66-NH 2 , forming diazonium salts that led to the quenching of blue fluorescence. With this strategy, a good linear relationship between NO 2 – concentration and the fluorescent intensity ratio of the nanoprobe in the range of 1–100 μM was established, with a detection limit of 0.021 μM. This dual-readout nanosensor was applied to analyze the concentration of NO 2 – in real meat samples, achieving satisfactory recovery rates of 94.72–104.52%, highlighting the practical potential of this method. Furthermore, a portable Gel/Rh6G@UIO-66-NH 2 hydrogel test kit was constructed for on-spot dual-mode detection of NO 2 – . This kit allows for convenient colorimetric analysis and fluorometric detection when used in conjunction with a smartphone. All the photos taken with the portable kit was converted into digital information using ImageJ software. It provides colorimetric and fluorescent visual detection of NO 2 – over a range of 0.1–1.5 mM, achieving a direct quantitative tool for NO 2 – identification. This methodology presents a promising strategy for NO 2 – detection and expands the application prospects for on-spot monitoring of food safety assessment.
研究领域:
分析化学
·
食品科学
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）