Advanced Healthcare Materials
·
2024
·
IF 10
Shuairu Zhu,Hongwei Xu,Wanying Guo,Mei Yang,Huiling Tan,Shaosong Hou,Jie Yao,Han Luo,Yongchao Yao,Jingxiang Zhao,Yonggang Wei,Xuping Sun,Binwu Ying
The versatile element composition and multifunctional properties of biodegradable silicates have attracted significant attention in cancer therapeutics. However, their application as nanozymes is often limited by suboptimal catalytic efficiency and insufficient intratumoral retention. In this study, the hydrothermal synthesis of iron silicate (FeSi) nanosheets are reported exhibiting exceptional peroxidase (POD)-like activity (136.7 U mg −1 ), outperforming most reported iron-based nanozymes. Density functional theory calculations revealed that the introduction of Si into the catalyst enhances H 2 O 2 adsorption and dissociation of Fe sites, leading to superior POD performance. Furthermore, the FeSi nanosheets are modified with Indocyanine Green (ICG) to facilitate targeted aggregation-potentiated therapy. The integration of ICG improved tumor penetration and retention of the FeSi nanosheets, significantly increasing their reactive oxygen species production and bolstering therapeutic efficacy.
研究领域:
生物医学工程
·
癌症治疗
·
纳米医学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE RESEARCH
·
2021
·
IF 2.104
Xinyan He,Jun Hong,Shengping Liu,Defeng Xu,Hang Hu
In the present work, hydroxyethyl starch-new indocyanine green (HES-IR-820) conjugates were developed for enhanced cancer photodynamic therapy . HES-IR-820 conjugates were prepared by the condensation reaction between IR-820 and amino groups modified HES. HES-IR-820 conjugates with IR-820 loading content of 2.0% (HES-IR-820 2.0 ) and 3.2% (HES-IR-820 3.2 ) were prepared and characterized by 1 H NMR, FT-IR, HPLC, and UV–Vis. HES-IR-820 2.0 and HES-IR-820 3.2 are monomolecular nanosized particles with hydrodynamic diameters of around 10 nm. HES-IR-820 2.0 and HES-IR-820 3.2 exhibit significantly enhanced stability in pH 7.4 PBS buffer and pH 7.4 PBS buffer containing 10% fetal bovine serum as compared to free IR-820. HES-IR-820 2.0 and HES-IR-820 3.2 show limited drug release in pH 7.4 and pH 5.0 PBS buffer. HES-IR-820 2.0 and HES-IR-820 3.2 exhibit enhanced singlet oxygen generation under 808 nm laser irradiation and reduced cellular uptake amount as compared to free IR-820. The cellular uptake pathway study reveals that the lipid raft-mediated endocytosis and macropinocytosis are involved in the cellular uptake of HES-IR-820 2.0 and HES-IR-820 3.2 . Compared to free IR-820, HES-IR-820 2.0 and HES-IR-820 3.2 show reduced cytotoxicity, enhanced in vitro antitumor effect under 0.3 W/cm 2 808 nm laser irradiation, and similar in vitro antitumor effect under 0.6 W/cm 2 808 nm laser irradiation. HES-IR-820 conjugates show significant potential for cancer photodynamic therapy.
研究领域:
肿瘤学
·
药剂学
·
生物医学工程
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Xu Cheng,Yingran Xu,Yong Zhang,Chaochao Jia,Bing Wei,Ting Hu,Rupei Tang,Conghu Li
Nanoscale hydroxyapatite (nHA) is considered as a promising drug carrier or therapeutic agent against malignant tumors. But the strong agglomeration tendency and lack of active groups seriously hamper their usage in vivo. To address these issues, we fabricated an organic–inorganic hybrid nanosystem composed of poly(acrylic acid) (PAA), nHA, and indocyanine green (ICG), and further modified with glucose to give a targeting nanosystem (GA@HAP/ICG-NPs). These hybrid nanoparticles (∼90 nm) showed excellent storage and physiological stability assisted by PAA and had a sustained drug release in an acidic tumor environment. In vitro cell experiments confirmed that glucose-attached particles significantly promoted cellular uptake and increased intracellular ICG and Ca2+ concentrations by glucose transporter 1 (GLUT1)-mediated endocytosis. Subsequently, the excessive Ca2+ induced cell or organelle damage and ICG triggered photothermal and photodynamic effects (PTT/PDT) under laser irradiation, resulting in enhanced cell toxicity and apoptosis. In vivo tests revealed that the hybrid nanosystem possessed good hemocompatibility and biosafety, facilitating in vivo circulation and usage. NIR imaging further showed that tumor tissues had more drug accumulation, resulting in the highest tumor growth inhibition (87.89%). Overall, the glucose-targeted hybrid nanosystem was an effective platform for collaborative therapy and expected to be further used in clinical trials.
研究领域:
纳米医学
·
肿瘤学
·
生物材料学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2020
·Vol. 21
·期 13
·4789
·
IF 4.556
Yiyu Wang,Chunqing Niu,Sisi Fan,Yuwei Li,Xiang Li,Yujun Dai,Jian Shi,Xinyu Wang
Photothermal therapy possesses great advantages for the treatment of drug-resistant tumors. Herein, Near Infrared (NIR)-triggered photothermal nanoparticles were developed through loading indocyanine green (ICG), a kind of NIR dye, into amino group-modified silica nanoparticles (SiO2-NH2NPs). SiO2-NH2NPs were prepared with immobilization of the amino groups into the framework of silica nanoparticles (SiO2NPs) by employing (3-aminopropyl)-triethoxysilane (APTES). Before and after the modification of the amino group, the particle sizes of SiO2NPs showed similar value, around 100 nm. ICG was further adsorbed into SiO2-NH2NPs by electrostatic attraction to enable SiO2-NH2@ICG NPs as a kind of photothermal agent. The loading rate of ICG to SiO2-NH2was greatly increased compared to unmodified SiO2, and the stability of ICG was also improved. Moreover, the SiO2-NH2@ICG NPs exhibited efficient photothermal effects due to ICG transforming laser power into local heat through the connected ICG, when NIR laser irradiation turned on for a couple of minutes. Finally, the in vitro antitumor efficacy of SiO2-NH2@ICG NPs was investigated by recording cell proliferation rate and further chronicled the apoptotic morphology evidence by a Calcein-AM/PI fluorescent staining assay, indicating the efficient photothermal targeted therapy for the HepG2 tumor cells.Keywords:mesoporous silica nanoparticles;indocyanine green;NIR-triggered;photothermal effect;tumor cells
研究领域:
纳米技术
·
癌症治疗
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材料科学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2021
·
IF 5.455
Sheng Wu,Renlu Han,Yuyan Yan,Wei Chen,Wenqing Gao,Yafei Hou,Keqi Tang
Recently, catalytic therapy on the basis of Fenton reaction represents a very promising paradigm for potential cancer therapy, which has inspired great research interest and got fast progress. Nevertheless, it remains challenging to fabricate nanocatalytic systems that can precisely perform catalytic activity in tumor tissues without causing toxicity to adjacent normal tissues. Herein, we designed and fabricated a porous CeO 2 nanorods that can selectively perform therapeutic activity in the weakly acidic tumor microenvironment via converting H 2 O 2 into •OH to kill cancer cells, meanwhile, the normal tissues are protected by decomposing H 2 O 2 into O 2 in the physiological environment. Specifically, benefiting from the porous structure, such CeO 2 nanorods could be loaded with Indocyanine green (ICG) that could both contribute itself to generate singlet oxygen ( 1 O 2 ) and convert near-infrared (NIR) light to hyperthermia. Once exposed to NIR light, on the one hand, the catalytic activity of CeO 2 was significantly enhanced by photothermal effect of ICG for highly efficient tumor catalytic therapy; on the other hand, the generated hyperthermia and 1 O 2 by ICG can further synergistically enhance the therapy efficiency via directing killing cancer cells. The current study provides a proof-of-concept demonstration of tumor-specific therapy with minimal non-targeted toxicity.
研究领域:
癌症治疗
·
纳米技术
·
材料科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
Contrast Media & Molecular Imaging
·
2019
·
IF 1.984
Yuxiao Xia,Li Zhang,Yanhong Zhao,Xiangdong Liu,Liang Cai,Lin Liu,Yue Chen,Wei Zhang
Objective. This work evaluated the potential of 68Ga-labelledNOTA-ICG (1,4,7-triazacyclononane-1,4,7-triacetic acid indocyanine green) for liver reserve imaging. Methods. To determine the optimal conditions for generating 68Ga-NOTA-ICG, various reaction parameters were implemented. Quality control analysis was performed using different chromatography techniques. The in vitro and in vivo stability was also measured at specific time points. The radioactivity ratio between n-octanol and water was determined to evaluate the water solubility of 68Ga-NOTA-ICG. The plasma-protein binding rate of the labelled compound was determined by the methanol method. The biodistribution and imaging findings were evaluated in normal animals at different time points after injection. A preliminary imaging evaluation was performed using an animal model of hepatic ischaemia-reperfusion injury, which was confirmed by pathology. Results. 68Ga-NOTA-ICG was prepared with very high radiochemical purity (>98%) by reacting at 90°C for 10 min at pH = 3.5∼4.0, with excellent stability in vivo and in vitro (>95% 3 h postpreparation). The in vitro plasma-protein binding rate of 68Ga-NOTA-ICG was 13.01 ± 0.7%, and it showed strong water solubility . We found that in addition to excretion through the biliary tract and intestines, 68Ga-NOTA-ICG can be excreted through the urinary tract. The image quality of 68Ga-NOTA-ICG was very high; imaging agent retained in the area of liver injury could clearly be observed. Conclusion. This is the first report on a 68Ga-labelled NOTA-ICG fragment for liver reserve function studies. This complex has promise as a candidate agent for liver reserve imaging.
研究领域:
医学影像学
·
核医学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
Science China-Materials
·
2021
·Vol. 65
·期 4
·1076-1086
·
IF 8.273
Fan Shuaikang,Wang Shilin,Wang Xiaobin,Wan Xinyi,Fang Zhou,Pi Xiaodong,Ye Zhizhen,Peng Xinsheng
Proton conduction controlled by light is important for advanced applications in sensors and information processing devices. We herein report a metal-organic framework (MOF) thin film by introducing indocyanine green dye into HSB-W5 (HSB = hydrogenated Schiff base) matrix (ICG@HSB-W5) to construct a series of basic logic gates (NOT, NAND, and NOR) on account of its good photothermal conversion ability and photo-controlled proton conductivity. The proton conductivity of ICG@HSB-W5 thin film is 25 times higher than that of the pristine HSB-W5 thin film and responses efficiently to lasers. In addition, the change of proton conductivity is very large with an ON/OFF ratio above 1000 and enough to be distinguished to build logic gates. In this logic gate system, 808- and 405-nm lasers are used as inputs and the proton conductivity is used as output signal with different thresholds. The electrical output signal is convenient and available to be received and detected. It offers a new route for the design of molecular logic gates based on MOF and facilitates the construction of proton-based logic gate or more complicated logic devices.
研究领域:
材料科学
·
纳米科技
·
物理化学
·
分子器件
·
生物传感器
✓ 已验证
DOI
高影响因子
Discover Nano
·
2025
·Vol. 20
·期 1
·1-17
·
IF 4.5
Hu Dongmei,Wu Qing,Yang Yujun,Wang Yan,Li Yanhao,Chen Haixiang,Tang Liang,Mao Xiang,Wang Zhenyu
Metal-based nanoenzymes with excellent biocompatibility and stable chemical properties are an effective antimicrobial agent against bacterial resistance due to their radical-mediated catalysis. In this work, due to the pH of most bacterial infection sites being close to neutral, targeting the problem of Fe 3 O 4 @Ag difficulty in maintaining the catalytic activity of nanoenzymes in neutral environments, we prepare a novel multifunctional Fe 3 O 4 @Ag@ indocyanine green/adenosine triphosphate peroxidase nanoenzymes for synergistic antibacterial activity. ICG (Indocyanine Green) and ATP (Adenosine triphosphate) are adsorbed on the surface of Fe 3 O 4 @Ag through electrostatic adsorption to form its structure. The cell viability remained above 90%, indicating its good biocompatibility. By complexing ATP with nanoenzymes to participate in single electron transfer and binding with Fe (II), ATP promotes the sudden release of hydroxyl radical (·OH) from the system, successfully transferring Fe 3 O 4 @Ag the peroxidase activity of nanoenzymes extends to neutral pH. By utilizing ICG as a photosensitizer and a sonosensitizer, under the combined treatment of near-infrared light and ultrasound, the photodynamic therapy (PDT)/photothermal therapy (PTT)/sonodynamic therapy (SDT) functions can be achieved, achieving multifunctional synergistic antibacterial effects. In a neutral environment, its bactericidal efficiency against Gram negative ( Escherichia coli ) and Gram positive ( Staphylococcus aureus ) is 99.9% and 99.7%, respectively, providing a new multi-mode synergistic antibacterial strategy for bacterial infections. Graphical abstract Metal based nanoenzymes with excellent biocompatibility and stable chemical properties are an effective antimicrobial agent against bacterial resistance due to their radical mediated catalysis. The prepared multifunctional Fe 3 O 4 @Ag@ICG/ATP exhibit peroxidase nanoenzymes perform ance for synergistic antibacterial activity. ICG and ATP are adsorbed on the surface of Fe 3 O 4 @Ag through electrostatic adsorption to form its structure
研究领域:
生物医学工程
·
纳米医学
·
抗菌材料研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2025
·
IF 5.5
Suyao Yin,Denver P. Linklater,Zheyu Li,Shuai He,Zechang Li,Shuai Xiang,Zenglian Zong,Dongmei Li,Liping Wen,Xu Shi,Saulius Juodkazis,Kai Sun,Elena P. Ivanova,Lei Jiang
Phototherapy treatment, as an alternative to antibiotic therapy, promises to be a noninvasive strategy to treat bacterial infections with a low likelihood of inducing bacterial resistance. However, the prolonged high temperature induced by photothermal therapy (PTT) and the limited reactive oxygen species (ROS) yield generated by photodynamic therapy (PDT) still limit clinical application. Here, we overcome the limitations of current PTT and PDT technologies through the effect of the red-shifted local surface plasmon resonance (LSPR) and oxygen vacancies to synthesize an indocyanine green-decorated gold nanoparticle/manganese dioxide (APIM) nanocomposite, improving photothermal stability and ROS generation. Under 808 nm laser irradiation, APIM nanocomposites achieved antibacterial efficiencies of up to 99.9% against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli and 99% against tetracycline-resistant E. coli EIEC. Furthermore, the near-infrared (NIR)-mediated APIM nanocomposite could effectively treat S. aureus-infected wound in vivo without marked toxicity. Overall, synergistic PTT and PDT utilizing the APIM nanocomposite may provide a promising treatment for skin wound infection.
研究领域:
医学
·
纳米生物学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
You Li,Wei Liu,Ying Li,Tao Peng,Jing Wang,Tao Zou
Human serum albumin (HSA)-indocyanine green (ICG)-paclitaxel (PTX) nanoparticles have been prepared for tumor photothermal-photodynamic-chemotherapy, which was inevitably internalized by normal cells. Here, hyaluronic acid (HA)-HSA-ICG-PTX nanoparticles were designed for active targeting mediated tumor multimodal therapy. HA as a ligand imparted the characteristics of the nanoparticles to specifically target the CD44 receptors overexpressed tumor cells, while enhancing the stability of the nanoparticles. HSA-ICG-PTX@HA nanoparticles were selectively internalized by CD44 overexpressed cervical and breast cancer (Hela and MCF-7) cells, rather than normal (293) cells lacking the receptor. Moreover, compared with HSA-ICG-PTX nanoparticles, HSA-ICG-PTX@HA nanoparticles exhibited improved tumor cell uptake and cytotoxicity, as well as enhanced photothermal effects and tumor suppressor effects. Therefore, tumor-targeting, human serum protein-based nanoparticles entirely consisting of FDA-approved compounds were demonstrated for fluorescent-photoacoustic-photothermal imaging mediated multimodal tumor therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano-Micro Letters
·
2018
·Vol. 10
·期 4
·1-15
·
IF 7.381
Xue Peng,Yang Ruihao,Sun Lihong,Li Qian,Zhang Lei,Xu Zhigang,Kang Yuejun
Indocyanine green (ICG) is capable of inducing a photothermal effect and the production of cytotoxic reactive oxygen species for cancer therapy. However, the major challenge in applying ICG molecules for antitumor therapy is associated with their instability in aqueous conditions and rapid clearance from blood circulation, which causes insufficient bioavailability at the tumor site. Herein, we conjugated ICG molecules with Prussian blue nanoparticles enclosing a Fe 3 O 4 nanocore, which was facilitated by cationic polyethyleneimine via electrostatic adsorption. The nanocarrier-loaded ICG formed stable aggregates that enhanced cellular uptake and prevented fluorescence quenching. Moreover, the strong superparamagnetism of the Fe 3 O 4 core in the obtained nanocomposites further improved cellular internalization of the drugs guided by a localized magnetic field. The therapeutic efficacy of this nanoplatform was evaluated using tumor models established in nude mice, which demonstrated remarkable tumor ablation in vivo due to strong photothermal/photodynamic effects. This study provides promising evidence that this multifunctional nanoagent might function as an efficient mediator for combining photothermal and photodynamic cancer therapy.
研究领域:
纳米技术
·
癌症治疗
·
生物医学工程
·
材料科学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Guanghui Hou,Junmin Qian,Min Guo,Weijun Xu,Jinlei Wang,Yaping Wang,Aili Suo
It is still a huge challenge for concurrent highly efficient loading of chemotherapeutic agent and photosensitizer into single nanocarrier via stimuli-responsive linkages due to their different physicochemical properties and pharmacokinetics . Herein, based on the discovery of unique cisplatin-hydrazide and cisplatin-indocyanine green (ICG) coordination reactions, a multifunctional coordination nanoprodrug, cisplatin/ICG co-loaded hydrazided hyaluronan/bovine serum albumin (HBCI) nanoparticles , was developed by a desolvation-dual coordination process. The nanoprodrug exhibited ultrahigh drug loading efficiency and glutathione/NIR light dual-responsive drug release behavior. In vitro cellular studies demonstrated efficient internalization and apoptosis-inducing ability of the nanoprodrug in HepG2 cells. In vivo results confirmed the efficacious tumor accumulation and biosafety of HBCI nanoprodrug and synergistic effect of HBCI-based combined photodynamic chemotherapy on inhibiting tumor growth. Overall, this work not only provides a novel dual coordination approach for highly efficient loading of cisplatin and ICG but also verifies the therapeutic potential of HBCI nanoprodrug in combating hepatocellular carcinoma.
研究领域:
药学
·
肿瘤学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）