Jiao Zhou,Xinyue Lin,Shuangshuang Liu,Zhanbin Wang,Dongchao Liu,Yonghong Huo,Dehai Li
Inonotus hispidushas various health-promoting activities, such as anticancer effects and immune-stimulating activity. The commercialization of valuable plant triterpenoids faces major challenges, including low abundance in natural hosts and costly downstream purification procedures. In this work, orthogonal design was used to compound methyl jasmonate (MeJA), salicylic acid (SA), oleic acid, and Cu2+, and the effects of combinations on the total triterpenes biosynthesized were studied. The optimal combination was screened out and its effect on the activity of PAL, CAT, and SOD was studied. The optimal concentration of oleic acid was 2% when MeJA was 100 mol/L, and the total triterpenoid content and mycelia production were 3.918 g and 85.17 mg/g, respectively. MeJA treatment induced oxidative stress, and at the same time increased the activity of related defense enzymes. Oleic acid is thought to regulate cell permeability by recombining cell membranes. It promotes the material exchange process between cells and the environment without affecting cell growth. When oleic acid was used in combination with MeJA, a synergistic effect on triterpene production was observed. In conclusion, our findings provide a strategy for triterpenoid enrichment ofI. hispidus.Keywords:Inonotus hispidus;combination of elicitors;defense enzyme;triterpenoid
Xiaopeng Liu,Qiuyi Wang,Jie Wang,Lin Guo,Yanhai Chu,Changyang Ma,Wenyi Kang
A new polysaccharide (IHP-1aa) was isolated from the fruiting body of Inonotus hispidus by hot water extraction, ethanol precipitation and column chromatography . The molecular weight of IHP-1aa was 26.9 kDa. Structural analysis showed that IHP-1aa consisted of glucose (Glc), galactose (Gal), fucose (Fuc), mannose (Man) and contained a certain amount of 3- O -methylgalactose (3- O -Me-Gal). The structure was mainly composed of →6)- α / β -D-Glc p -(1→, →6)- α -D-Gal p -(1→, →6)-(3- O -Me)- α -D-Gal p -(1→, →6)- α- D-Man p -(1 → and →2, 6)- α -D-Gal p -(1 → as the main chain. Branched at O-2 with single β -L-Fuc p -(1 → 6)- α -D-Gal p -(1 → 6)- α -D-Glc p -(1 → as major the side chain. The results of SEM, XRD and AFM combined with Congo red indicated that IHP-1aa may be amorphous granular chain conformation. In addition, IHP-1aa stimulated macrophage function and improved phagocytic ability of RAW264.7, as well as promoted the secretion of NO , TNF-α and IL-6. IHP-1aa, a 3- O -methylgalactose-containing heteropolysaccharide, was isolated for the first time from the I. hispidus , which may be used as a potential immunomodulator in functional foods.
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