INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2016
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IF 3.138
Hong Niu,Dan Song,Haibo Mu,Wuxia Zhang,Feifei Sun,Jinyou Duan
Mushroom Inonotus obliquus ( I. obliquus ), a folk medicine, has been widely used to treat several human malicious tumors since 16th century. In this study, three homogenous biomolecules (designated IOA1, IOA2 and IOA3) were prepared from the alkali extract of I. obliquus . Their molecular weights were measured to be 6.1 × 10 4 , 2.9 × 10 4 and 3.5 × 10 4 g/mol respectively and all of them were characterized as lignin–carbohydrate complexes mainly comprised lignin as well as −25% carbohydrates. Antioxidant assays indicated that all of them exhibited pronounced reductive power and strong scavenging activities on DPPH and hydroxyl radicals in vitro . Immunological tests showed that they could also significantly stimulate nitric oxide production and phagocytic activity in RAW 264.7 macrophages. These results implied that the lignin–carbohydrate complexes extracted from I. obliquus might be used as novel natural antioxidants or immunostimulants in functional foods or pharmaceutical candidates.
研究领域:
药理学
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生物化学
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产品关联
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2024
·
IF 2.3
Hao Xie,Wenwen Li,Linghe Zhang,Nurmamat Eliyas
A novel polysaccharide, Inonotus obliquus polysaccharide (IOP), was extracted using a microwave extraction method and subsequently subjected to modifications through sulfation, carboxymethylation, phosphorylation, and acetylation. Its physical and chemical properties were analyzed using various chemical techniques, including high-pressure liquid chromatography, ultraviolet light, Fourier-transform infrared spectroscopy, X-ray diffraction, Congo red test, and scanning electron microscopy. The antioxidant capacity was assessed using DPPH, ABTS, and hydroxyl radical assays, as well as by measuring the reducing power. Additionally, hypoglycemic activity was evaluated through α-glucosidase and α-amylase assays. The results indicated that the chemical modifications effectively altered the physical and chemical properties, as well as the biological activities of IOP. Compared to the unmodified IOP, the derivatives exhibited reduced sugar content, uronic acid content, and molecular weight, while demonstrating varying levels of antioxidant and hypoglycemic capabilities. Notably, the carboxymethylated IOP (IOP−C) displayed lower molecular weight, higher ABTS free radical scavenging rate, greater reducing ability, and increased α-amylase inhibition rate. Therefore, IOP−C shows promise as a potential edible antioxidant and hypoglycemic agent. Graphical
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Fungal Biology
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2022
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IF 2.91
Rong Zhou,Hanbing Yang,Ting Lu,Yanxia Zhao,Weifa Zheng
Production of hispidin polyphenols in Inonotus obliquus is a stress-induced response triggered by environmental factors. As one of the important environmental factors, ultraviolet (UV) radiation plays regulatory roles in fungal growth and development . However, whether UV radiation regulates the formation of hispidin polyphenols remains to be established. In this study, we cultivated I. obliquus on solid medium and imposed intermittent UV radiation. We showed that UV exposure inhibited the growth of mycelia but increased the production of polyphenols. Further bioassays revealed that UV radiation also increased the catalytic activities of phenylalanine ammonia-lyase (PAL) and chalcone isomerase (CHI), up-regulated expression of genes related to redox, transcriptional regulation, and metabolism. In addition, the total extracts from the UV-irradiated group were more capable of scavenging DPPH and ABTS + free radicals, especially at the later stage of culture. Thus, UV radiation, acting as one of the environmental factors, stimulated the accumulation of polyphenols in I. obliquus by regulating the activities of enzymes and the expression of genes related to growth and metabolism, and can be tentatively used as a feasible strategy to enhance the production of polyphenols in I. obliquus .
研究领域:
真菌学
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药理学
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环境生物学
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DOI
PubMed
高影响因子
Journal of Saudi Chemical Society
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2023
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IF 5.6
Ruilin Hao,Yafeng Li,Shuhua Shan,Haihua Xu,Junyan Li,Zhuoyu Li,Rongshan Li
Polyphenols containing styrene pyranone skeleton are unique to porous fungi . Inonotus obliquus ( IO ) is a medicinal and edible porous fungus. Twelve phenolic compounds containing four styryl pyranone polyphenols from IO were isolated and identified in this work. The antioxidant ability of the isolates was characterized utilizing the ferric reducing antioxidant power (FRAP) assay and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) radical scavenging test. Styrylpyranone polyphenols, especially methylinoscavin B, could capture free radicals better than other phenolic compounds, and quantum chemical calculations also confirmed this evaluation. The density functional theory (DFT) calculation data showed that Styrylpyranone polyphenols, especially methylinoscavin B, have a lower energy gap, higher softness and higher electronic chemical potential than other phenolic compounds. The bond dissociation energy values of the bond in C7ʹ O–H of the methylinoscavin B molecule are less than those in C11 and C6ʹ O–H when reacting with ∙OOH (selected as a representative free radical). On the basis of calculations, hydrogen atom transfer (HAT) is supposed to be a preferred mechanism over single electron transfer (SET) when phenolic compounds react with free radicals. Moreover, after treatment with final concentrations of 0.5, 1, 2, 3, 4 and 5 μM phelligridin E (PHE), the activity of SOD1 increased by 70.15%, 11.36%, 145.45%, 172.73%, 205.05% and 275.23%, respectively. The molecular dynamics simulation (MD) study of PHE employed SOD1 (PDB ID: 6FN8). The MD results showed that the hydrogen bonds between ASP147 of SOD1 and PHE promote GLU223-ARG224 to form a stable C coil after combining with PHE. The formation of the C Coil enhanced the stability of the electrostatic loop (EL) of SOD1 and the rate of diffusion of the superoxide anion to the active site. Styrylpyrone polyphenols of Inonotus obliquus origin have the potential to be a source of vigorous free radical scavengers and antioxidant enzyme activators.
研究领域:
生物化学
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计算化学
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药理学
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产品关联
DOI
高影响因子
CHEMISTRY & BIODIVERSITY
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2025
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IF 2.5
Hao Xie,Nurmamat Eliyas
To address the low extraction yield and structural degradation issues of conventional methods, this study optimized microwave-assisted extraction (61°C, 64 min, 30:1 mL/g ratio) to obtain Inonotus obliquus polysaccharides (IOP), followed by DEAE-52 and Sephadex G-100 purification to isolate two fractions: neutral IOP-1 (12.67 kDa) and acidic IOP-2 (26.13 kDa). Monosaccharide analysis revealed both as heteropolysaccharides containing mannose, glucose, and galactose with distinct compositional ratios. Structural characterization showed IOP-1 primarily contains 1,4-Glcp, 1,3,6-Glcp, and 1,3-Manp linkages, while IOP-2 features 1,3-Glcp, 1,4-Glcp, 1,4,6-Glcp, and 1,4-Galp linkages. Multiple antioxidant assays demonstrated superior free radical scavenging capacity in both polysaccharides, with IOP-1 exhibiting exceptional activity exceeding typical polysaccharides performance. These findings highlight IOP-1's potential as a novel natural antioxidant for pharmaceutical and food applications. Graphical
研究领域:
食品科学
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药学
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生物化学
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天然产物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
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2023
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IF 7.7
Caili Guo,Fangcong Hua,Peiying Chen,Xiaoyin Wang,Xiaohong Hou,Jianhua Qu,Qi Hu
Herbal medicine is used widely, and the residues increase rapidly. Biochar preparation from the residues is a potential way to treat wastes with wastes. Inonotus obliquus (IO) is a natural porous fungus and is famous for its hypoglycemic effect. The residues of Inonotus obliquus (IOR) are potential raw materials for biochar because of their porous structure. P-IORBC and Zn-IORBC, activated by H 3 PO 4 and ZnCl 2 respectively, were prepared from IOR. The distinct properties of P-IORBC and Zn-IORBC were shown in the study. P-IORBC was optimized by response surface method (RSM), and the optimized parameters of P-IORBC preparation were 166 min (pyrolysis time), 374 °C (pyrolysis temperature), and 3.91 (the mass ratio of H 3 PO 4 to IOR). The actual methylene blue (MB) adsorption (951.56 mg/g) was near the predicted value (971.00 mg/g), indicating the reliability of the response surface model. Zn-IORBC has a larger pore volume and pore size, while P-IORBC has a larger specific surface area. The specific surface areas of Zn-IORBC and P-IORBC were 1676.78 and 2014.51 m 2 /g, respectively. Compared to Zn-IORBC, the pyrolysis temperature of P-IORBC was reduced from 547 °C to 374 °C. The tetracycline (TC) adsorption for P-IORBC and Zn-IORBC was 1515.46 mg/g and 1033.66 mg/g. P-IORBC was more effective at the adsorption of cationic dyes than Zn-IORBC. In general, P-IORBC had more potential due to its high yield and environmentally friendly characteristics. The data provides a reference for biochar preparation from residues of herbal medicine.
研究领域:
环境科学
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生物技术
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2024
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IF 8.5
Lixiang Fang,Junchen Li,Xiaoxiao Chen,Xiangqun Xu
Lignocellulose constitutes the primary component of dietary fiber. We assessed how fermenting bamboo shoot residue with the medicinal white-rot fungus Inonotus obliquus affected the yield, composition, and functional attributes of dietary fiber by altering bamboo shoot residue lignocellulose's spatial structure and composition. I. obliquus secretes lignocellulolytic enzymes, which effectively enhance the degradation of holocellulose and lignin by 87.8% and 25.5%, respectively. Fermentation led to a more porous structure and reduced crystallinity. The yield of soluble dietary fiber increased from 5.1 g/100 g raw BSR to 7.1 g/100 g 9-day-fermented bamboo shoot residue. The total soluble sugar content of dietary fiber significantly increased from 9.2% to 13.8%, which improved the hydration, oil holding capacity, in vitro cholesterol, sodium cholate, and nitrite adsorption properties of dietary fiber from bamboo shoot residue. These findings confirm that I. obliquus biotransformation is promising for enhancing dietary fiber yield and quality.
研究领域:
食品科学
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微生物学
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生物技术
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产品关联
DOI
PubMed
高影响因子
MOLECULES
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2021
·Vol. 26
·期 9
·2467
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IF 4.412
Yueqi Wang,Liping Guo,Chunming Liu,Yuchi Zhang,Sainan Li
Extracts of the fungusInonotus obliquusexhibit cytotoxic properties against different cancers; hence, this fungal species has been extensively studied. This study aimed to extract total triterpenoids fromInonotus obliquususing ionic liquids (ILs) and separate potential lactate dehydrogenase (LDH) inhibitors via ultrafiltration (UF)-high-speed countercurrent chromatography (HSCCC). Total triterpenoids fromInonotus obliquuswere extracted by performing a single-factor experiment and employing a central composite design via ultrasonic-assisted extraction (UAE) and heat-assisted extraction (HAE). The extract was composed of 1-butyl-3-methylimidazolium bromide as the IL and methanol as the dispersant. Ultrafiltration-liquid chromatography (UF-LC) was used to rapidly scan the LDH inhibitors and betulin and lanosterol were identified as potential inhibitors. To obtain these target compounds, betulin and lanosterol with the purities of 95.9% and 97.8% were isolated from HSCCC within 120 min. Their structures were identified using several techniques, among which IL-HAE was fast and effective. This study reports the extraction of triterpenoids fromInonotus obliquusby IL for the first time. Collectively, the findings demonstrate that UF-LC is an effective tool for screening potential LDH inhibitors from crude extracts ofI. obliquusand may help to identify bioactive substances against myocardial infarction, whereas high-purity compounds can be separated via UF-HSCCC.Keywords:Inonotus obliquus;ultrafiltration;liquid chromatography;ultrasonic-assisted extraction;lactate dehydrogenase inhibitor;myocardial infarction
研究领域:
药理学
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分析化学
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中药学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
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2021
·Vol. 46
·期 3
·e13932
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IF 2.72
Lin-song Luo,Yu Wang,Li-jun Dai,Fang-xia He,Jiu-liang Zhang,Qing Zhou
The purpose of this study was to explore the hypouricemic effect in hyperuricemia mice of triterpenoid acids from Inonotus obliquus (TAIO), and decipher of the underlying xanthine oxidase inhibitory mechanism. Measurement of xanthine oxidase (XO) inhibitory activity was assayed. Organ indexes and serum biochemical indicators were measured in potassium oxonate-induced hyperuricemia mice. Studies showed that TAIO had the strong inhibitory effect on XO activity, and its inhibition type was mixed and reversible. In vivo, TAIO decreased efficiently uric acid level, hepatic XO, serum blood urea nitrogen activities in hyperuricemia mice. Indicating that TAIO may ameliorate kidney damage and relieve inflammation in hyperuricemic mice, and had the inhibitory effect on XO activity. Furthermore, eight triterpenoids were identified by Ultra performance liquid chromatography electrospray quadrupole time of flight mass spectrometry. These findings proved that triterpenoids from Inonotus obliquus would have potential biological characteristics and effect on controlling hyperuricemia and gout as an active supplement. Practical applications There are a large amount of evidence indicating that hyperuricemia and gout are related to the hypertension and obesity. And gout and hyperuricemia are also possible connection with cardiovascular disease and metabolic syndrome. Currently, xanthine oxidase is the target of many kinds of chemical drugs at present, but the therapeutic drugs used in clinical medicine will produce more or less side effects. Therefore, the aim of this study was to explore the material basis of effective substances for reducing uric acid in Inonotus obliquus and to evaluate its effect. This study can provide a promising application of Inonotus obliquus in the fields of functional foods or medicines for gout and hyperuricemia.
研究领域:
药理学
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药物化学
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营养保健品
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Pharmacology
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2023
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IF 5.6
Sun Runbin,Jin Dandan,Fei Fei,Xu Zhi,Cao Bei,Li Juan
Introduction: Polysaccharides from Grifola frondosa (Dicks.) Gray (HSH) and Inonotus obliquus (Fr.) Pilat (BHR) showed noticeable effects on dextran sulfate sodium (DSS)-induced colitis, but their systemic modulation effects have not been fully revealed. This study aimed to investigate the regulation of the gut microbiota and systemic metabolism by HSH and BHR in DSS-induced colitis.Methods: C57BL/6J mice were given DSS (2.5%) in water and were treated with HSH and BHR (200 mg/kg/day) by gavage. Body weight and colon length were recorded, and H&E and AB-PAS staining of the colon were conducted to evaluate the model and the protective effect of the polysaccharides. Additionally, an LC-QTOF/MS-based untargeted metabolomic platform was used to identify the metabolites in the serum, colon tissue, gut contents, and faeces and investigate differential metabolites and metabolic pathways. 16S rDNA gene sequencing was used to measure the composition of bacterial communities.Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively). HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc. HSH and BHR increased the Chao1 index by 64.25% and 60.25%, respectively, and they increased the Shannon index by 13.02% and 10.23%, respectively. They both reversed the increase in the abundances of g_Odoribacter, g_Clostridium, g_AF12, g_Parabacteroides and g_Turicibacter and reversed the decrease in the abundance of g_unclassified_Bacteria induced by DSS. Specifically, HSH reversed the reductions in g_unclassified_Lactobacillales and g_Ruminococcus, and BHR reversed the decreases in g_unidentified_Coriobacteriaceae and g_unclassified_Firmicutes.Discussion: These results suggested that HSH and BHR may ameliorate DSS-induced colitis by global modulation of systemic metabolism and the gut microbiota. Targeting the gut microbiota may be a potentially effective strategy to modulate systemic metabolism and treat colitis.
研究领域:
药理学
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微生物学
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代谢组学
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胃肠病学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）